항진균성 펩티드에 의한 표고버섯 푸른곰팡이병의 억제

이형진 ( Hyoung-jin Lee ),윤영배 ( Yeong-bae Yun ),허정훈 ( Jeong-hoon Huh ),김영기 ( Young-kee Kim ) 한국응용생명화학회 2017 Journal of Applied Biological Chemistry (J. Appl. Vol.60 No.2

원목과 톱밥배지에서 푸른곰팡이의 오염과 성장은 표고버섯의 생장을 심하게 저해할 수 있다. 표고버섯 재배에서 푸른곰팡이 병의 방제를 위하여 농약과 항생제와 같은 화학 약품의 사용은 일반적으로 허용되지 않는다. 본 연구에서는 푸른곰팡이병의 방제를 위하여 느타리버섯에서 갈반병을 일으키는 세균성 병원균을 분리하고 이들의 펩티드 독소를 분리하였다. Pseudomonastolaasii 균주들은 항진균 활성을 갖는 톨라신 및 이와 구조적유사체인 다양한 펩티드 독소를 분비한다. 이러한 펩티드들은 표고버섯의 생장에는 영향을 주지 않는다는 것이 알려져 있다. 펩티드 독소들을 Trichoderma harzianum H1 푸른곰팡이에 처리하였을 때, 푸른곰팡이의 성장을 저해하였다. 펩티드를 분비하는 20 종의 P. tolaasii 균주 중에서 강, 중, 약의 항진균 활성을 가진 균주의 수는 각각 8, 5, 7 종으로 나타났다. 표고버섯을 재배하는 동안에, 펩티드 독소를 함유한 세균 배양액에서 세균을 제거한 배양원액을 톱밥배지 표면에 자란 푸른곰팡이 균사에 분무하였다. 배양원액은 푸른곰팡이의 성장은 억제할 수 있는 반면, 표고버섯 생장과 생산에는 영향을 주지 않았다. 펩티드 독소를 함유하는 배양원액은 곰팡이의 흰색 균사체를 노란색의 마른 딱지로 변화시켰고, 이것은 펩티드 독소가 강력한 항진균 활성과 살균 활성을 갖고 있음을 보여준다. Contamination and growth of Trichoderma, a green mold, on the oak log and wooden chip or sawdust media can severely inhibit the growth of oak mushroom. Chemicals including pesticides and antibiotics are generally not allowed for the control of green mold disease during mushroom cultivation. In this study, bacterial pathogens causing blotch disease on the oyster mushrooms were isolated and their peptide toxins were purified for the control of green mold disease. Strains of Pseudomonas tolaasii secret various peptide toxins, tolaasin and its structural analogues, having antifungal activities. These peptides have shown no effects on the growth of oak mushrooms. When the peptide toxins were applied to the green mold, Trichoderma harzianum H1, they inhibited the growth of green molds. Among the 20 strains of peptide-forming P. tolaasii, strong, moderate, and weak antifungal activities were measured from 8, 5, and 7 strains, respectively. During oak mushroom cultivation, bacterial culture supernatants containing the peptide toxins were sprayed on the aerial mycelia of green molds grown on the surface of sawdust media. The culture supernatants were able to suppress the fungal growth of green molds while no effect was observed on the mushroom growth and production. They changed the color of molds from white aerial mycelium into yellowish dried scab, representing the powerful anti-fungal and sterilization activities of peptide toxins.

익산 미륵사지 녹유연목와의 제작기술과 의미

송현경 백제학회 2018 백제학보 Vol.0 No.26

본고에서는 미륵사지 녹유연목와의 형식별 특징과 제작기법을 검토하고 출토 양상을 살펴보았다. 녹유연목와는 일찍부터 연판의 형태와 테두리 문양을 기준으로 A형과 B형으로 분류되었고, A형이 절대다수를 차지한다. A형은 문양면을 만드는 내범과 외범이 결합된 와범으로 제작되었으며, B형은 내범만을 이용해 제작하였다. 외범의 형태는 자방 둘레 문양으로 볼 때 원형임을 확인하였다. 녹유연목와 제작과정 중 소성에 관한 부분은 재벌구이가 이루어졌을 가능성도 배제할 수 없다. 녹유는 문양면과 테두리에만 시유하였으며 백제에서 6세기부터 다양한 기종에 시유되었던 녹유가 7세기 대에 기와에 시유된 것은 수당에서 유행한 녹유기와의 영향으로 생각된다. 녹유연목와는 금당과 목탑지보다 회랑과 강당, 승방지에서 주로 출토되는 양상은 미륵사식수막새 2단계인 F-3형, G형과 유사하다. 2단계 기와는 서원 석탑 주변 성토층에서 B형과 G형이 함께 발견된 것에 근거하여 639년 이후에 주로 제작된 것으로 추정되는데, 녹유연목와 역시 이와 비슷한 시기에 제작되었을 것으로 추정된다. 동아시아에서 연목와와 녹유의 양상을 살펴보았을 때, 백제 연목와는 대통사식수막새와 마찬가지로 중국 남조의 영향을 받아 제작되었지만 성립과정에서 내재화면서 중국과 달리 주연부가 없는 형태가 유행한 것으로 보인다. 그리고 주변국에 영향을 주었는데 일본 최초의 가람인 아스카데라의 Ⅰ·Ⅱ형식 연목와에서 잘 나타나고 있다. 녹유 기와의 경우 경주의 월성, 안압지, 사천왕사지 등지에서도 출토되고 일본의 平安宮유적에서도 확인된다. 외범 역시 7세기 후반 이후 신라와 일본 고대 사원 유적에서 확인되고 있다. 이는 백제의 기와 제작 기술이 백제 멸망 이후에도 신라와 일본에 지속적으로 영향을 미쳤음을 보여준다고 할 수 있다. The main discussion of this article is focused on the formal characteristics, manufacturing technique, and excavations of the green-glazed Rafter-end tiles excavated at the Mireuksa Temple site. Green-glazed Rafter-end tiles are largely divided into two types, Type A and Type B, according to the shapes of the lotus petals and the edge, of which Type A constitute the great majority. The Type A tiles were made using a tile mold consisting of an “inner mold” to make the part of the tile bearing the ornamental design, and an “outer mold”, while the Type B tiles were made using only an inner mold. The outer mold must have been circular considering the design surrounding the ovary. Studies also suggest that the tiles were baked in a double-firing process. Green glaze was applied only to the ornamental face and the edge. It is believed that the use of green glaze, which began to be applied widely to the ceramic vessels of Baekje in the sixth century and to roof tiles in the seventh century, was influenced by the popularity of green-glazed tiles in Sui and Tang China. The excavation circumstances of the green-glazed Rafter-end tiles discovered at the sites of the galleries, lecture hall and dormitory rather than the sites of the central dharma hall and wooden pagodas are similar to those of Type F-3 and Type G convex Rafter-end tiles, which belong to the “second-stage tiles” of the Mireuksa Style. These second-stage tiles of Type B and Type G are presumed to have been manufactured after 639, as they were discovered in the solum around the stone pagoda in the Western Sanctuary. It suggests that the green-glazed Rafter-end tiles were also made during the same period. Considering the aspects of the Rafter-end tiles and green glaze used in East Asia, it seems that the Rafter-end tiles of Baekje were produced under the influence of the Southern Dynasties of China, as suggested by the convex Rafter-end tiles unearthed at the Daetongsa Temple site. But the tiles began to acquire their own unique form once the local tile makers started manufacturing them, resulting in the popularity of the lotus design without the edge ornament unlike the Chinese tiles. The Rafter-end tiles of Type I and Type II discovered at the Asuka-dera, the earliest Buddhist temple in Japan, show the influence of the Baekje tiles. Green-glazed tiles have been discovered at various archaeological sites, including the Wolseong, Wolji pond and Sacheonwangsa Temple sites in Gyeongju and the Heian Palace site in Japan. Outer tile molds have also been found at several temple sites of Silla and Japan that were founded after the late seventh century. This suggests that the tile manufacturing technology of Baekje continued to influence Silla and Japan even after the fall of the Baekje kingdom in 660.

Development and mechanical properties of bagasse fiber reinforced composites

Cao, Yong,Goda, Koichi,Shibata, Shinichi The Korean Society for Composite Materials 2007 Advanced composite materials Vol.16 No.4

Environment-friendly composites reinforced with bagasse fiber (BF), a kind of natural fiber as the remains from squeezed sugarcane, were fabricated by injection molding and press molding. As appropriate matrices for injection molding and press molding, polypropylene (PP) and polycaprolactone-cornstarch (PCL-C) were selected, as a typical recyclable resin and biodegradable resin, respectively. The mechanical properties of BF/PP composites were investigated in view of fiber mass fraction and injection molding conditions. And the mechanical properties and the biodegradation of BF/PCL composites were also evaluated. In the case of injection molding, the flexural modulus increased with an increase in fiber mass fraction, and the mechanical properties decreased with an increase in cylinder temperature due to the thermal degradation of BF. The optimum conditions increasing the flexural properties and the impact strength were $90^{\circ}C$ mold temperature, 30 s injection interval, and in the range of 165 to $185^{\circ}C$ cylinder temperature. On the other hand, as to BF/PCL-C fully-green composites, both the flexural properties and the impact strength increased with an increase in fiber mass fraction. It is considered that the BF compressed during preparation could result in the enhancement in mechanical properties. The results of the biodegradability test showed the addition of BF caused the acceleration of weight loss, which increased further with increasing fiber content. This reveals that the addition and the quantities of BF could promote the biodegradation of fully-green composites.

Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii

( Song Hee Lee ),( Hwa Jin Jung ),( Seung-beom Hong ),( Jong In Choi ),( Jae-san Ryu ) 한국균학회 2020 Mycobiology Vol.48 No.4

In Pleurotus sp., green mold, which is considered a major epidemic, is caused by several Trichoderma species. To develop a rapid molecular marker specific for Trichoderma spp. that potentially cause green mold, eleven Trichoderma species were collected from mushroom farms and the Korean Agricultural Culture Collection (KACC). A dominant fungal isolate from a green mold-infected substrate was identified as Trichoderma pleuroticola based on the sequences of its internal transcribed spacer (ITS) and translation elongation factor 1- α (tef1) genes. In artificial inoculation tests, all Trichoderma spp., including T. atroviride, T. cf. virens, T. citrinoviride, T. harzianum, T. koningii, T. longibrachiatum, T. pleurotum, and T. pleuroticola, showed pathogenicity to some extent, and the observed symptoms were soaked mycelia with a red-brown pigment and retarded mycelium regeneration. A molecular marker was developed for the rapid detection of wide range of Trichoderma spp. based on the DNA sequence alignment of the ITS1 and ITS2 regions of Trichoderma spp. The developed primer set detected only Trichoderma spp., and no cross reactivity with edible mushrooms was observed. The detection limits for the PCR assay of T. harzianum (KACC40558), T. pleurotum (KACC44537), and T. pleuroticola (CAF-TP3) were found to be 500, 50, and 5 fg, respectively, and the detection limit for the pathogen-to-host ratio was approximately 1:10,000 (wt/wt).

볏짚배지에 탄산칼슘의 처리가 느타리버섯에 미치는 영향

전창성 외 한국버섯학회 2004 한국버섯학회지 Vol.2 No.2

This study was carried out to investigate effect of CaCO₃ treatment on cultivation of oyster mushroom for suppression of green mold disease and for promotion of mycelial growth to stabilize mushroom production in field and laboratory experiment. Treatment of CaCO₃ in PDA media promoted mycelial growth of mushroom and suppressed that of green mold. Addition of CaCO₃ in rice straw substrate increased mushroom mycelial growth compared with control. In that case, growth of green mold increased up to treated 0.6% CaCO₃ but decreased in treatment beyond 0.8% CaCO₃. There were some differences on effect of CaCO₃ treatment according to green mold species. Trichoderma longibrachiatum was effected but T. virens was not effected by treated CaCO₃. Differences among mushroom strains by treated CaCO₃ were not shown. It is confirmed that treatment of CaCO₃ can promote mushroom mycelial growth but it's not clear in the field. In the result of field test, treatment of CaCO₃ in rice straw substrates tended to increase yield and decrease incidence of disease compared with non-treatment. These results suggest that CaCO₃ treatment on cultivation of oyster mushroom can be applied to take preventive steps against of green mold disease. 느타리버섯재배에서 심각한 피해를 주고 있는 푸른곰팡이병을 억제하고 버섯균사의 생장을 촉진하여 버섯의 안정생산을 도모하기 위한 탄산칼슘의 처리효과를 실내 및 실외시험을 실시한 결과. PDA배지에서의 CaCO₃의 처리는 버섯균의 균사생장을 촉진하고, 병원균은 억제되었으며, 볏짚배지상의 CaCO₃의 처리에서는 버섯균은 무처리에 비하여 균사생장이 증가되었으며, 병원균 대체적으로 0.6%의 처리까지는 증가되나 0.8%처리부터는 감소되는 경향을 나타냈다. 컬럼 내의 볏짚배지에서의 Trichoderma longibrachiatum의 접종구에서는 CaCO₃의 처리효과가 있었으나 T. virens의 접종구에서는 CaCO₃의 효과가 없었다. CaCO₃의 처리에 따른 느타리버섯 품종간의 균사생장의 차이는 없었다. CaCO₃는 처리에 따라 느타리버섯의 균사생장은 촉진하고 푸른곰팡이병원균과 붉은빵곰팡이균에 대해 약간의 억제 능력은 인정되었다. 포장시험 결과 수량성은 무처리구에 비하여 높으며, 발병율은 낮은 경향이나 절대적인 방제효과는 없었다.

볏짚배지에 탄산칼슘의 처리가 느타리버섯에 미치는 영향

전창성,공원식,장갑열,유영복,도은수,천세철 한국버섯학회 2004 한국버섯학회지 Vol.2 No.2

This study was carried out to investigate effect of CaCO3 treatment on cultivation of oyster mushroom for suppression of green mold disease and for promotion of mycelial growth to stabilize mushroom production in field and laboratory experiment. Treatment of CaCO3 in PDA media promoted mycelial growth of mushroom and suppressed that of green mold. Addition of CaCO3 in rice straw substrate increased mushroom mycelial growth compared with control. In that case, growth of green mold increased up to treated 0.6% CaCO3 but decreased in treatment beyond 0.8% CaCO3. There were some differences on effect of CaCO3 treatment according to green mold species. Trichoderma longibrachiatum was effected but T. virens was not effected by treated CaCO3. Differences among mushroom strains by treated CaCO3 were not shown. It is confirmed that treatment of CaCO3 can promote mushroom mycelial growth but it's not clear in the field. In the result of field test, treatment of CaCO3 in rice straw substrates tended to increase yield and decrease incidence of disease compared with non-treatment. These results suggest that CaCO3 treatment on cultivation of oyster mushroom can be applied to take preventive steps against of green mold disease.

Control of Citrus Green and Blue Molds by Chinese Propolis

Shuzhen Yang,Litao Peng,Yunjiang Cheng,Siyi Pan,Feng Chen 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.5

Green and blue molds, caused by Penicillium digitatum and Penicillium italicum, respectively, are economically important postharvest diseases of citrus fruits. In this study, Chinese propolis ethyl acetate extract (PEAE) was evaluated to control P. digitatum and P. italicum on postharvest citrus fruits. The results indicated PEAE strongly inhibited mycelia growth and induced hyphae prominent abnormal morphological alterations. Also, PEAE had strong detrimental effect on spore germination of the tested pathogens in a concentrationdependent manner. For in vivo tests, PEAE could both reduce decay caused by P. digitatum and P. italicum respectively in wound-inoculated fruit and naturally infected fruit; meanwhile, no negative influences on the overall quality of the citrus fruits were observed with PEAE treatment. Therefore, PEAE could be used as a natural antifungal agent to control citrus blue and green mold.

Highlighting the Microbial Community of Kuflu Cheese, an Artisanal Turkish Mold-Ripened Variety, by High-Throughput Sequencing

Talha Demirci 한국축산식품학회 2024 한국축산식품학회지 Vol.44 No.2

Kuflu cheese, a popular variety of traditional Turkish mold-ripened cheeses, is characterized by its semi-hard texture and blue-green color. It is important to elucidate the microbiota of Kuflu cheese produced from raw milk to standardize and sustain its sensory properties. This study aimed to examine the bacteria, yeasts, and filamentous mold communities in Kuflu cheese using high-throughput amplicon sequencing based on 16S and ITS2 regions. Lactococcus, Streptococcus, and Staphylococcus were the most dominant bacterial genera while Bifidobacterium genus was found to be remarkably high in some Kuflu cheese samples. Penicillium genus dominated the filamentous mold biota while the yeasts with the highest relative abundances were detected as Debaryomyces, Pichia, and Candida. The genera Virgibacillus and Paraliobacillus, which were not previously reported for mold-ripened cheeses, were detected at high relative abundances in some Kuflu cheese samples. None of the genera that include important food pathogens like Salmonella, Campylobacter, Listeria were detected in the samples. This is the first experiment in which the microbiota of Kuflu cheeses were evaluated with a metagenomic approach. This study provided an opportunity to evaluate Kuflu cheese, which was previously examined for fungal composition, in terms of both pathogenic and beneficial bacteria.

일본의 도래계 사원과 백제 유민의 동향 2

이병호 한국고대학회 2013 先史와 古代 Vol.39 No.-

This article discusses recent archaeological research on early Buddhist temples related to the immigrants from the Korean peninsula located in the Asuka, Kawachi, Yamashiro, Kyūshū regions in order to understand their influence on Japanese Buddhist temples after the fall of the Baekje kingdom. The second section deals with the seventh century sites in the Asuka region. Of particular importance is the type of roof tiles made with bamboo-patterned molds excavated from the Asukadera’s southeastern meditation precinct, which was established by Dojou, who was from an immigrant clan. A particular attention is given to their relation with the roof tiles discovered in the regional mountain fortresses in Baekje. In addition, it is pointed out that the layout of palace, workshops, and ponds in the Asuka region is similar to those in the Iksan and Buyeo areas. Baekje-related features are also found in Kawaradera. They include clay sculptures and green-glazed artifacts. Moreover, Kawaradera was founded by transforming a palace complex into a Buddhist temple, and in its plan the monks’ quarters were located in the north, east, and west sides of the lecture hall. It is notable that their parallels can be found in Baekje. In the case of Hinokumadera, the most important immigrants-related temple in Asuka, the roof tile foundation and roof tiles decorated with flame design discovered at the site are comparable to the finds in Baekje. Considering that the date of the temple is presumed as the latter half of the seventh century, it is likely that the Baekje immigrants participated in the construction of the temple. The third section examines the temples in the Kawachi, Yamashiro, Katsuragi, and Kyūshū regions. In the case of the Kawachi region, the temple plans and roof-end tiles found in Shindou Haichi, Sairinji(clan temple of Wanni’s descendants), and Senshouji show their close connection to the Baekje temples. The Yamashiro region is known to have been linked more closely with Silla and Gorguyeo than Baekje. In this context, it is notable that the roof tile foundation, which is one of the major Baekje-related feature, was discovered in the octagonal pagoda site in Kadagihara Haichi associated with Hatauchi and also in Komadera, whose establishment is believed to have been related to certain Goguyeo clans. This suggests a possibility that the transmission and distribution of the roof tile foundation was more complicated that previously assumed. The Baekje immigrants’ contribution to the region’s Buddhist culture is tangible in Katsuragi, where clay plaques with Buddhist images were found in the Nikoji Haichi site. In the case of Asatsuma Haichi, the wooden slips from Asukaike site serve as significant soucres for the history of the Baekje immigrants. In the Kyūshū area, gilt-bronze Buddhist images and roof-end tiles were found in the Korean-style mountain fortresses, and they provide more detailed information on the Baekje immigrants after the fall of the Baekje kingdom. 이 글은 백제 멸망 이후 일본의 고대사원에 영향을 미친 백제 유민의 동향과 관련하여 飛鳥・河內・山背・九州 지역의 도래계 사원에 관한 최근의 고고학적 연구 성과를 정리한 것이다. Ⅱ장에서는 7세기대 飛鳥 지역의 도래계 사원에 대해 검토하였다. 도래계 씨족 출신인 道昭가 개창한 飛鳥寺의 東南禪院에서는 竹狀模骨을 이용한 기와가 확인되는데 이러한 기와와 백제 지방산성에서 출토된 기와의 관련성을 검토하였다. 또 飛鳥 지역의 왕궁과 공방, 연못 등의 배치가 익산이나 부여 지역의 그것과 유사하다고 보았다. 川原寺에 대해서는 왕궁이 사원으로 바뀐 점이나 삼면 승방을 가진 가람배치, 소조상, 녹유제품 등이 백제와 연관된 것으로 생각된다. 飛鳥의 대표적인 도래계 사원인 檜隈寺의 경우 와적기단을 비롯하여 火炎文瓦當 등이 백제 지역에서 출토된 유적・유물들과 연관되며, 시기적으로 7세기 후반대에 속하기 때문에 백제 유민의 활동과 연관될 수 있다고 보았다. Ⅲ장에서는 河內・山背・葛城・九州 지역의 도래계 사원에 대해 검토하였다. 河內 지역의 경우 新堂廢寺를 비롯하여 王仁의 후예 씨족 사원인 西琳寺, 善正寺에서 출토된 와당들이 백제의 가람배치나 와당과 연관될 가능성을 검토하였다. 山背 지역의 경우 백제보다는 신라나 고구려와의 관련성이 더 높은 지역이다. 秦氏와 관련이 깊은 樫原廢寺의 팔각형 목탑지와 고구려계 씨족과 관련이 깊은 高麗寺에서는 백제 사원과 관련이 깊은 와적기단이 발견되었다. 따라서 이러한 와적기단의 도입 과정이나 그 계통, 확산 과정이 매우 복잡한 경로를 통해 이루어졌을 가능성을 언급하였다. 葛城 지역의 경우 二光寺廢寺에서 출토된 전불을 통해 백제 유민의 구체적인 활동 모습을 추정할 수 있고, 朝妻廢寺의 경우 飛鳥池遺蹟에서 출토된 목간을 통해 도래계 씨족들의 활동 모습을 추정할 수 있다. 九州 지역의 경우 조선식산성 내부에서 출토된 금동불이나 와당과 같은 자료의 검토를 통해 백제 멸망 이후 유민의 활동에 대한 보다 더 구체적인 추정이 가능하다는 점을 언급하였다.

일본의 도래계 사원과 백제 유민의 동향 2 -비조(飛鳥), 기타 지역의 고고학 성과를 중심으로-

( Byong Ho Lee ) 한국고대학회 2013 先史와 古代 Vol.39 No.-

이 글은 백제 멸망 이후 일본의 고대사원에 영향을 미친 백제 유민의 동향과 관련하여 飛鳥 河內山背九州 지역의 도래계 사원에 관한 최근의 고고학적 연구 성과를 정리한 것이다. Ⅱ장에서는 7세기대 飛鳥 지역의 도래계 사원에 대해 검토하였다. 도래계 씨족 출신인 道昭가 개창한 飛鳥寺의 東南禪院에서는 竹狀模骨을 이용한 기와가 확인되는데 이러한 기와 와 백제 지방산성에서 출토된 기와의 관련성을 검토하였다. 또 飛鳥 지역의 왕궁과 공방, 연못 등의 배치가 익산이나 부여 지역의 그것과 유사하다고 보았다. 川原寺에 대해서는 왕궁 이 사원으로 바뀐 점이나 삼면 승방을 가진 가람배치, 소조상, 녹유제품 등이 백제와 연관된 것으로 생각된다. 飛鳥의 대표적인 도래계 사원인 檜외寺의 경우 와적기단을 비롯하여 火炎文 瓦當 등이 백제 지역에서 출토된 유적 유물들과 연관되며, 시기적으로 7세기 후반대에 속하기 때문에 백제 유민의 활동과 연관될 수 있다고 보았다. Ⅲ장에서는 河內山背葛城九州 지역 의 도래계 사원에 대해 검토하였다. 河內 지역의 경우 新堂廢寺를 비롯하여 王仁의 후예 씨족 사원인 西琳寺, 善正寺에서 출토된 와당들이 백제의 가람배치나 와당과 연관될 가능성을 검토 하였다. 山背 지역의 경우 백제보다는 신라나 고구려와의 관련성이 더 높은 지역이다. 秦氏와 관련이 깊은 견原廢寺의 팔각형 목탑지와 고구려계 씨족과 관련이 깊은 高麗寺에서는 백제 사원과 관련이 깊은 와적기단이 발견되었다. 따라서 이러한 와적기단의 도입 과정이나 그 계통, 확산 과정이 매우 복잡한 경로를 통해 이루어졌을 가능성을 언급하였다. 葛城 지역의 경우 二光寺廢寺에서 출토된 전불을 통해 백제 유민의 구체적인 활동 모습을 추정할 수 있고, 朝妻廢寺의 경우 飛鳥池遺蹟에서 출토된 목간을 통해 도래계 씨족들의 활동 모습을 추정할 수 있다. 九州 지역의 경우 조선식산성 내부에서 출토된 금동불이나 와당과 같은 자료의 검토를 통해 백제 멸망 이후 유민의 활동에 대한 보다 더 구체적인 추정이 가능하다는 점을 언급하였다. This article discusses recent archaeological research on early Buddhist temples related to the immigrants from the Korean peninsula located in the Asuka, Kawachi, Yamashiro, Kyushu regions in order to understand their influence on Japanese Buddhist temples after the fall of the Baekje kingdom. The second section deals with the seventh century sites in the Asuka region. Of particular importance is the type of roof tiles made with bamboo-patterned molds excavated from the Asukadera`s southeastern meditation precinct, which was established by Dojou, who was from an immigrant clan. A particular attention is given to their relation with the roof tiles discovered in the regional mountain fortresses in Baekje. In addition, it is pointed out that the layout of palace, workshops, and ponds in the Asuka region is similar to those in the Iksan and Buyeo areas. Baekje-related features are also found in Kawaradera. They include clay sculptures and green-glazed artifacts. Moreover, Kawaradera was founded by transforming a palace complex into a Buddhist temple, and in its plan the monks` quarters were located in the north, east, and west sides of the lecture hall. It is notable that their parallels can be found in Baekje. In the case of Hinokumadera, the most important immigrants-related temple in Asuka, the roof tile foundation and roof tiles decorated with flame design discovered at the site are comparable to the finds in Baekje. Considering that the date of the temple is presumed as the latter half of the seventh century, it is likely that the Baekje immigrants participated in the construction of the temple. The third section examines the temples in the Kawachi, Yamashiro, Katsuragi, and Kyushu regions. In the case of the Kawachi region, the temple plans and roof-end tiles found in Shindou Haichi, Sairinji(clan temple of Wanni`s descendants), and Senshouji show their close connection to the Baekje temples. The Yamashiro region is known to have been linked more closely with Silla and Gorguyeo than Baekje. In this context, it is notable that the roof tile foundation, which is one of the major Baekje-related feature, was discovered in the octagonal pagoda site in Kadagihara Haichi associated with Hatauchi and also in Komadera, whose establishment is believed to have been related to certain Goguyeo clans. This suggests a possibility that the transmission and distribution of the roof tile foundation was more complicated that previously assumed. The Baekje immigrants` contribution to the region`s Buddhist culture is tangible in Katsuragi, where clay plaques with Buddhist images were found in the Nikoji Haichi site. In the case of Asatsuma Haichi, the wooden slips from Asukaike site serve as significant soucres for the history of the Baekje immigrants. In the Kyushu area, gilt-bronze Buddhist images and roof-end tiles were found in the Korean-style mountain fortresses, and they provide more detailed information on the Baekje immigrants after the fall of the Baekje kingdom.