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자궁경부암 수술 후 림프절 전이가 없는 경우 미세전이 발견을 위한 면역 조직 화학적 검사의 임상적 의의
김정식,이해혁,남계현,김희경,배동한 대한부인종양학회 2006 Journal of Gynecologic Oncology Vol.16 No.3
OBJECTIVE: The purpose of this study was to identify, by immunohistochemistry, possible micrometastasis in the pelvic lymph nodes previously considered free by conventional histopathological examination, and to assess their influence on the survival of patients with cervical cancer. METHODS: This retrospective study included patients(n=51) operated on between February 2001 and May 2004 for cervical cancer without histopathologic lymph node involvement. Lymph nodes(n=282) from 51 patients with histologically node-negative cervical cancer were evaluated for micrometastasis. These were submitted to immunohistochemical study using AE1/AE3 anti-cytokeratin monoclonal antibodies to identify neoplastic epithelial cells. RESULTS: Lymph node micrometastases were immunohistochemically detected in 3 of the 51 patients(5.9%), comprising 3 of 282(1.1%) pelvic lymph nodes examined. One patient(Ib2) had adenocarcinoma and others(Ib1,Ib2), squamous cell carcinoma. All of them had negative lymphovascular space invasion. In three patients, there were recurrences(66.7%,2/3), and one patient(Ib1) died from the pelvic recurrence. CONCLUSIONS: We recommend an immunohistochemical examination for lymph node micrometastases in cervical cancer patients with histologically negative nodes. Micrometastasis could provide important information for further treatment strategies and follow up. Its clinical significance in cervical cancer warrants further study. 목적: 자궁 경부암으로 수술후 조직학적 검사에서 골반 임파절 전이가 없는 것으로 보고된 경우, 면역조직화학적인 방법으로 미세전이의 유무를 확인후 이에 대한 의미와 예후와의 관계를 분석하고자 하였다. 연구 방법: 2001년 2월부터 2004년 5월 까지 본원에서 자궁경부암으로 근치적 자궁적출술 및 골반 임파절 절제술을 받은 79명의 환자들을 대상으로 하였다. 이들중 병리조직학적 검사에서 골반 임파절 전이가 없는 것으로 보고된51명의 골반 임파절들을 면역 조직화학적으로 검사하였으며, 총 282개의 골반 임파절 슬라이드를 anti-cytokeratin antibodies AE1/AE3 (DakoCytomation,California,USA)로 염색하였다. 결과: 자궁경부암 병기별 환자들의 분포는 1a1: 7 명,Ia2: 4명,Ib1: 29명,Ib2: 5명,IIa: 5명,IIb: 1명이며,환자들의 평균나이는 46.3세(27-64세)였다. 면역 조직화학적인 방법으로 검사한 임파절들에서 미세전이는 51명중에서 3명(5.9%),전체 임파절 282개중 3개(1.1%)에서 발견되었다. 한명(Ib2)은 조직학적 유형으로 선암이었고, 나머지 2명(Ib1,Ib2)은 편평세포암이었다. 미세전이가 있는 3명 모두 임파혈관 침윤(lymph-vascular space invasion)은 없었다. 이들 3명중 2명에서만 재발이 되었고, 한 명(Ib1)은 재발로 인하여 사망하였다. 결론: 자궁 경부암 환자에서 수술 후 미세전이 유무가 향후 재발 요인 및 예후에 대한 예측인자로서 가능성을 보이며, 수술 후 항암 방사선 요법등의 치료 방침 설정 시에도 도움을 줄 것이라고 사료된다.
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o,p'-DDD가 흰쥐 뇌하수체 전엽의 ACTH분비의 활성도에 미치는 영향에 관한 면역조직화학적 연구
기근홍,최종범,임성철,박동수,이병래,전호종,서재홍,박규호 朝鮮大學校 附設 醫學硏究所 1989 The Medical Journal of Chosun University Vol.14 No.2
It has been well known that the toxicity of o,p'-DDD on the zona fasciculata in adrenal gland. The toxicity revealed adrenostatic effect on the cortisol biosynthesis by mitochodrial destruction. So, o,p'-DDD was used for unoperable adrenocortical carcinoma and its metastasis. The secretory activity of cortisol in adrenal cortex depended on ACTH secretion in the adenohypophysis. o,p'-DDD was dissolved in corn oil and administered orally for 28 days by 75 ㎎ / ㎏, 150㎎ / ㎏ and 300 ㎎ / ㎏ to investigate the adrenostatic effect on the cortisol biosynthesis of o,p'-DDD by assesment of ACTH secretory activity in anterior lobe of pituitary gland of rats. The results obtained were that the secretory activity of ACTH and the number of ACTH secretory cells were increased according to the dosage of o,p'-DDD, especially 300㎎ / ㎏ administrated group. Therefore, the suppression of cortisol biosynthesis of o,p'-DDD was confirmed by increased secretory activity of ACTH and the number of ACTH secretory cells.
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단세포군 항체를 이용한 사람태아의 OXYTOCIN과 VASOPRESSIN 분비세포에 관한 면역조직화학적 연구
정주현,장인엽,김종중,문정석,장신요,조사선,양희영 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.15 No.1
The study was to investigate the ontogeny of oxytocinergic and vasopressinergic neurons in the supraoptic and paraventhcular nuclei of the human fetal hypothalamus. The tissues of fetal hypothalami were fixd with 10% neutral buffered formalin and Bouin's solution, embedded in paraplast, and cut serially in coronal planes. Representive sections were stained with cresyl violet for the contour of both nuclei, and monoclonal antibodies to oxytocin and vasopressin were used for immunohistochemistry. The results observed under the light microscope were summarized as follows: 1. The oxytocinergic and vasopressinergic neurons were observed in both supraoptic and paraventnc ular nuclei, in addition, arcuate, suprachiasmatic. acessory supraoptic, Penfornical and penventncular nuclei. 2. In the fetal hvopthalami, oxytocinergic and vasopressinergic neurons were firstly observed at 19 th week of gestation. 3. The oxytocinergic and vasopressinergic neurons were mostly oval and round-shaped. 4. The ratio of the oxytocinergic and vasopressinergic neurons in the supraoptic nuclei to paraventricular nuclei were 19.8:1 and 65:1 respectively.
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소의 시상하부내에서 Vasopressin과 Oxytion분비세포의 분포에 관한 면역조직화학적 연구
유명철,김종중,장인엽,문정석,정주현,김흥중,조사선 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1
This study was done to observe the distributions of Oxytocinergic and Vasopressinergic neurons in the hypothalamus of the cow. For the immunohistochemical staining, sections were reacted with the following sequence of solutions: monoclonal anti-vasopressin and anti-oxytocin, biotinylated anti-mouse IgG, avidin-biotin peroxidase complex, and Ni-DAB. The results observed under light microscope were summarized as follows. Our immunohistochemical investigations reveal both vasopressinergic and oxytocinergic neurons in the supraoptic nucleus(SON), paraventricular nucleus(PVN), accessory supraoptic nucleus (ASN), periventricular nucleus(PN), suprachiasmatic nucleus(SCN), and arcuate nucleus(AN), but these two types of neurons were mainly distributed in the PVN and SON and the ratio of the number of vasopressinergic neurons to that of oxytocinergic neurons was 1:1.30 in the PVN, and 1:1.00 in the SON. These two types of neurons were round, oval and spindle-shaped and cell size was 30-55um.
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