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Immunogenicity of the nanovaccine containing intimin recombinant protein in the BALB/c mice
Zahra Sadat Hosseini,Jafar Amani,Fahimeh Baghbani Arani,Shahram Nazarian,Mohammad Javad Motamedi,Fatemeh Shafighian 대한백신학회 2018 Clinical and Experimental Vaccine Research Vol.7 No.1
Purpose: Escherichia coli O157:H7 is one of the most important pathogens which create hemorrhagic colitis and hemolytic uremic syndrome in human. It is one of the most prevalent causes of diarrhea leading to death of many people every year. The first diagnosed gene in the locus of enterocyte effacement pathogenicity island is eae gene. The product of this gene is a binding protein called intimin belonging to the group of external membrane proteins regarded as a good stimulants of the immune system. Chitosan with its lipophilic property is an environmentally friendly agent able to return to the environment. Materials and Methods: Intimin recombinant protein was expressed in pET28a vector with eae gene and purification was performed using Ni-NTA and finally the recombinant protein was approved through western blotting. This protein was encapsulated using chitosan nanoparticles and the size of nanoparticles was measured by Zetasizer. Intimin encapsulated was prescribed for three sessions among three groups of oral, injection, and oral-injection using Chitosan nanoparticles. Challenge was performed for all three groups with 108E. coli O157:H7 bacteria. Results: Intimin produced by chitosan nanoparticles improves immunological responses through the adjuvant nature of chitosan nanoparticles. Chitosan may be used as a carrier for transportation of the prescribed vaccine. Among the mice, encapsulated intimin could be able to provide suitable titers of IgG and IgA by the aid of chitosan nanoparticles. Results of mice challenge showed that decreased the bacterial shedding significantly. Conclusion: Results showed that the chitosan nanovaccine with intimin protein may be used as a suitable candidate vaccine against E. coli O157:H7.
Mohammad Reza Safaee-Ardakani,Ashrafalsadat Hatamian-Zarmi,Seyedeh Mahdieh Sadat,Zahra Beagom Mokhtari-Hosseini,Bahman Ebrahimi-Hosseinzadeh,Hamid Kooshki,Jamal Rashidiani 한국섬유공학회 2019 Fibers and polymers Vol.20 No.12
The use of natural polymers and electrospinning as a new method of wound dressing production is one of thethings that can revolutionize the medical world. Due to the importance of wound healing and characteristics such as antiinflammatoryand antimicrobial properties, it is possible to use natural compounds such as fungi and metabolites derivedfrom them to produce wound dressing. In this study, schizophyllan (SPG) as an extracellular polysaccharide was extractedfrom Iranian Schizophyllum commune (NCBI MG761830) and then the silver nanoparticles (AgNPs) were produced by thein-situ method in 1.5 % SPG solution. Afterward, they were combined with polyvinyl alcohol 10 % (PVA) polymer tostrengthen the fiber structure. We investigated the properties of nanofibers containing PVA/SPG-AgNPs and PVA/SPG20 %. The physicochemical properties of two fibers were investigated by SEM, TEM, FTIR, contact angle, water uptake,nanoparticle release, and biological test (antibacterial, and MTT). The diameter of the nanofiber-containing the AgNPs wasabout 169 nm and the other nanofiber was about 212 nm. The highest inhibition of the growth of the bacterium by PVA/SPGAgNPsagainst E. coli and S. aureus was about 88.34 % and 64.7 %, respectively. The silver ion release from PVA/SPGAgNPsnanofibers was 21 μg/ml after fifth day. Both nanofibers had no toxic effect on L929 fibroblast cells.