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Kim, Ok-Tae,Bang, Kyong-Hwan,Shin, Yu-Su,Lee, Min-Jeong,Jung, Su-Jin,Hyun, Dong-Yun,Kim, Young-Chang,Seong, Nak-Sul,Cha, Seon-Woo,Hwang, Baik Springer 2007 Plant cell reports Vol.26 No.11
<P>Transformed root ('hairy root') cultures have been shown to be a good model for the study of many secondary metabolites. However, economically important compounds such as asiaticoside and madecassoside are produced in insignificant amounts in the root of Centella asiatica (L.) Urban. To overcome this problem, C. asiatica was transformed using Agrobacterium rhizogenes strain R1000 that harbors pCAMBIA1302 encoding the hygromycin phosphotransferase (hpt) and green fluorescence protein (mgfp5) genes and the hairy culture was coupled with elicitation technique. Hairy roots were obtained at a frequency of up to 14.1% from a tissue junction between the leaf and petiole. Abundant hairy roots were observed when co-cultivation of the plant with A. rhizogenes was done for 7 days (36.1%). Transformation was confirmed by PCR and Southern blot analyses. Five weeks after inoculation, no asiaticoside was detected in the hairy root samples. However, when 0.1 mM methyl jasmonate (MJ) was applied as an elicitor to the culture medium for 3 weeks, a large quantity of asiaticoside was generated (7.12 mg/g, dry wt). In the case of gene expression, 12 h after MJ treatment the expression of the CabAS (C. asiatica putative beta-amyrin synthase) gene in the hairy roots is significantly different from that of the control and this level of transcripts was maintained for 14 days. Our results showed that production of C. asiatica hairy roots could be optimized and the resulting cultures could be elicited with MJ treatment for enhanced production of asiaticoside.</P>
Park, Ok-Jin,Han, Ji Young,Baik, Jung Eun,Jeon, Jun Ho,Kang, Seok-Seong,Yun, Cheol-Heui,Oh, Jong-Won,Seo, Ho Seong,Han, Seung Hyun Society for Leukocyte Biology 2013 Journal of leukocyte biology Vol.94 No.6
<P><I>E. faecalis</I> lipoteichoic acid induces chemokine expression via TLR2/CD14/MyD88 and PAFR/JAK/STAT1 signaling pathways, without induction of IFN-β in murine macrophages.</P>
Im, Jintaek,Baik, Jung Eun,Kim, Kyoung Whun,Kang, Seok-Seong,Jeon, Jun Ho,Park, Ok-Jin,Kim, Hyun Young,Kum, Kee-Yeon,Yun, Cheol-Heui,Han, Seung Hyun Oxford University Press 2015 International immunology Vol.27 No.8
<P>LTA from <I>E. faecalis</I> inhibits LPS-induced IL-8 in human periodontal cells</P><P>Periodontitis is caused by multi-bacterial infection and <I>Aggregatibacter actinomycetemcomitans</I> and <I>Enterococcus faecalis</I> are closely associated with inflammatory periodontal diseases. Although lipopolysaccharide (LPS) of <I>A. actinomycetemcomitans</I> (Aa.LPS) and lipoteichoic acid of <I>E. faecalis</I> (Ef.LTA) are considered to be major virulence factors evoking inflammatory responses, their combinatorial effect on the induction of chemokines has not been investigated. In this study, we investigated the interaction between Aa.LPS and Ef.LTA on IL-8 expression in human periodontal ligament (PDL) cells. Aa.LPS, but not Ef.LTA, substantially induced IL-8 expression at the protein and mRNA levels. Interestingly, Ef.LTA suppressed Aa.LPS-induced IL-8 expression without affecting the binding of Aa.LPS to Toll-like receptor (TLR) 4. Ef.LTA reduced Aa.LPS-induced phosphorylation of mitogen-activated protein kinases, including ERK, JNK and p38 kinase. Furthermore, Ef.LTA inhibited the Aa.LPS-induced transcriptional activities of the activating protein 1, CCAAT/enhancer-binding protein and nuclear factor-kappa B transcription factors, all of which are known to regulate IL-8 gene expression. Ef.LTA augmented the expression of IL-1 receptor–associated kinase-M (IRAK-M), a negative regulator of TLR intracellular signaling pathways, in the presence of Aa.LPS at both the mRNA and protein levels. Small interfering RNA silencing IRAK-M reversed the attenuation of Aa.LPS-induced IL-8 expression by Ef.LTA. Collectively, these results suggest that Ef.LTA down-regulates Aa.LPS-induced IL-8 expression in human PDL cells through up-regulation of the negative regulator IRAK-M.</P>
Yoo, Nam-Hee,Kim, Ok-Tae,Kim, Jung-Bong,Kim, Sun-Hee,Kim, Young-Chang,Bang, Kyong-Hwan,Hyun, Dong-Yun,Cha, Seon-Woo,Kim, Min-Young,Hwang, Baik The Korean Society of Plant Biotechnology 2011 Plant biotechnology reports Vol.5 No.3
In order to produce centellosides from whole plant cultures of Centella asiatica (L.) Urban, we evaluated the synergistic effects of thidiazuron (TDZ) and methyl jasmonate (MJ) on whole plant growth and centelloside production. After 4 weeks of treatment with 0.025 mg/L of TDZ coupled with 0.1 mM MJ, the production of made-cassoside and asiaticoside from whole plant cultures was estimated to be 2.40- and 2.44-fold, respectively, above that of MJ elicitation alone. When whole plants were treated with a growth regulator and an elicitor, the growth of whole plants, as compared to the controls, did not differ. Additionally, total phytosyterol content in the leaves of whole plants co-treated with MJ and TDZ was 1.08-fold greater than those of MJ alone. These results demonstrate that combined treatments not only stimulate the accumulation of centellosides in the leaves but also inhibit the reduction of phytosterol levels caused by MJ elicitation.
간경변증 쥐 모델에서 혈청 Hyaluronic acid와 간섬유화의 정량적 측정치 간의 상관관계
김문영 ( Moon Young Kim ),백순구 ( Soon Koo Baik ),장윤옥 ( Yoon Ok Jang ),석기태 ( Ki Tae Suk ),김재우 ( Jae Woo Kim ),김현수 ( Hyun Soo Kim ),조미연 ( Mi Yun Cho ),최선주 ( Sun Joo Choi ),엄순호 ( Soon Ho Um ),한광협 ( Kwang Hyu 대한간학회 2008 Clinical and Molecular Hepatology(대한간학회지) Vol.14 No.2