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세균액 및 세균단백질 추출물이 배양 세포에 미치는 영향
유영대,임미경 대한치과보존학회 2000 Restorative Dentistry & Endodontics Vol.25 No.4
Dental pulp infection is most commonly caused by extensive dental caries, and some bacterial species invade root canals; bacterial components and products are thought to be associated with the pathogenesis of periapical periodontitis. A principle driving force behind pulpal disease response appears to lie in the host immune system's to bacteria and their products. We examined the production of interleukin 1β (IL-1β) and tumor necrosis factor α(TNF-α) from human peripheral mononuclear cells, lymphocytes and monocytes stimulated by heat-killed Acitnobacillus actinomycetemcomitans (ATCC 29523), Porphyromonas gingivalis (ATCC 33277) and Prevotella intermedia (ATCC 25611), and also by their sonicated bacterial extracts(SBE), respectively. The effects of three strains of heat-killed bacteria and their SBEs on the morphology of cultured blood cell lines HL-60 (KCLB 10240) and J774A.1 (KCLB 40067) were observed under the inverted microscope. Ultrastructural changes of J774A.1 exposed to heat- killed P. intermedia and its SBE were investigated using transmission electron microscopy. Production of IL-1β was reduced in human peripheral mononuclear cells after stimulation by sonic bacterial extracts of A. actinomycetemcomitans, P. gingivalis, and P. intermedia. Heat- killed and Sonic extract of P. gingivalis inhibited the production of TNF-α in peripheral mononuclear cells. Production of TNF-α was inhibited in peripheral monocytes after stimulation by sonic extracts of A. actinomycetemcomitans, P. gingivalis, and P. intermedia. HL-60 and J 774A.1 cells showed granular degeneration after treatment with heat- killed and sonic extracts of A. actinomycetemcomitans, P. gingivalis, and P. intermedia Chromatin margination and shrinkage were observed in 774A.1 treated with heat-killed P. intermedia. Cell wall structure and organelles were destroyed and vacuoles were formed in cytoplasm in J774A.1 treated with P. intermedia sonic extract. These results suggest that A. actinomycetemcomitans, P. gingivalis and P. intermedia may have an important role in the formation and progression of pulpal diseases via both modulation of production of IL-1β and TNF-α from blood mononuclear cells and cytopathic effects.
( Yu Jin Hong ),( Eung Gu Lee ),( Bo Mi Gil ),( Yong Hyun Kim ),( Sun Im ),( Hye Seon Kang ) 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.-
Ojbectives This study aimed to investigate whether thoracic skeletal muscle mass index at the diagnosis of aspiration pneumonia is a predictor for the recurrence of aspiration pneumonia and to explore predicting factors for the recurrence of aspiration pneumonia in stroke patients with dysphagia disorder. Methods We retrospectively reviewed the data of patients with aspiration pneumonia who diagnosed with dysphagia disorder due to stroke from Jan. 2014 to Jul. 2020 in The Catholic University of Korea Bucheon St. Mary’s Hospital. Aspiration pneumonia was defined based on clinical signs of symptoms suggestive of pneumonia and radiologic findings of pneumonic infiltrations in the dependent portion of lung. We measured thoracic muscle volume by using the cross-sectional area (CSA) of the erector spinae muscle (ESMCSA , cm2) at the 12th vertebral region. CT scans at the time of diagnosis of aspiration pneumonia were used for analysis and respective CSA were divided by height squared (m2) to yield the muscle index at T12 (T12MI, cm2/m2) to normalize for stature. Multivariate logistic regression models were performed to investigate relationships between clinical parameters and recurrence of aspiration pneumonia. Results During study period, a total of 268 stroke patients with dysphagia disorder and developed aspiration pneumonia were analyzed. Mean T12MI of patients with and without recurrence of aspiration pneumonia was 708.1±229.9 cm2/m2 and 622.3±184.1 cm2/m2, respsectively (P=0.001). Multivariate logistic regression revelaed that lower T12MI (P=0.038) and older age (P=0.007) were the independent predictor of recurrence of aspiration pneumonia in stroke patients with dysphagia disorder. Conclusion Low thoracic muscle index at the diagnosis of aspiration pneumonia predicts recurrence of aspiration pneumonia in stroke patients with dysphagia disorder.
유미희(Mi-Hee Yu),임효권(Hyo-Gwon Im),임남경(Nam-Kyung Im),황은영(Eun-Young Hwang),최준혁(Jun-Hyeok Choi),이은주(Eun-Ju Lee),김종부(Jong-Boo Kim),이인선(In-Seon Lee),서화정(Hwa-Jeong Seo) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.4
본 연구에서는 김치에서 분리한 유산균이 ACE 억제효과와 본태성 고혈압쥐의 혈압에 미치는 영향을 조사하였다. 김치에서 분리한 유산균의 배양액 및 lysozyme법, sonication법, solvent extract 법으로 균체를 추출하여 ACE 억제효과를 살펴본 결과, 배양액 보다는 균체를 이용하여 추출한 추출물에서 공시균주에 비해 ACE 억제효과가 높은 것으로 확인되었다. 균체 추출시료 중 LG 7, 8, 42가 전반적으로 높은 ACE 억제효과를 나타내어 이들의 균체를 본태성 고혈압쥐(SHR)에게 4주간 투여한 결과, WKY 대조군(I)이 4주간 81 g 정도의 체중 증가를 보인 반면, SHR 대조군(V)은 52 g 정도의 체중 증가를 보여 정상혈압 쥐에 비해 고혈압 쥐의 체중의 증가가 낮은 것으로 나타났으며, 유산균의 장기투여가 고혈압 쥐의 체중 및 장기무게에 유의적인 영향을 주지 않았다. 혈청 중 총 콜레스테롤 농도와 중성지질 농도는 SHR 대조군(V)에 비해 유산균 투여군(X, XI)에서 약간 감소하는 경향을 보였으나 유의적인 차는 아니었으며, SHR에 LG 42 유산균을 섭취시킴으로서 총 콜레스테롤과 중성지질의 농도는 감소하고, HDL 콜레스테롤의 농도는 증가하는 것으로 나타났다. 유산균 투여 후 4주째의 최종 혈압을 비교한 결과, LG 42를 투여함에 따라 기준혈압에 비해 농도 의존적(1×10sup{7}, 1×10sup{9} cfu/mL)으로 각각 12, 27% 감소하였다. This study was conducted to evaluate the anti-hypertensive effect of Lactobacillus sp. isolated from Kimchi by examining its effects on renal angiotensin-converting enzyme (ACE) inhibitory activity, lipid components and blood pressure using the spontaneously hypertensive rat (SHR) system. Most Lactobacillus sp. extracts (lysozyme, sonication and ethyl acetate extracts) showed higher capacities for the inhibition of ACE activity than those of cultured media. Particularly, LG 7, 8 and 42 of Lactobacillus sp. showed the strongest inhibitory activity among the Lactobacillus sp. extracts. The concentrations of total cholesterol and triglycerides in the serum were lower in the Lactobacillus sp. administration groups than in the control group, but these differences were not significant. The HDL-cholesterol concentrations of the LG 42 administration groups (IX, X) were significantly higher than that of the control group. At 4 weeks, the systolic blood pressure (SBP) in the LG 42 Lactobacillus sp. (1×109 cfu/mL) group (XI) was about 27% lower than that of the control group (V). No adverse effects were observed on the liver and there was no difference in the aspartate aminotransferase (AST) and alanine aminotransferase (ALT) values among groups. The results of this study suggest that long term consumption of LG 42 Lactobacillus sp. may be beneficial to the prevention of high blood pressure.
이직의도가 있는 임상간호사의 소진경험에 관한 현상학적 연구
김증임 ( Jeung Im Kim ),손행미 ( Haeng Mi Son ),박인희 ( In Hee Park ),신희진 ( Hee Jin Shin ),박지현 ( Ji Hyun Park ),조미옥 ( Mi Ock Cho ),김성의 ( Seong Ui Kim ),유미옥 ( Mi Ock Yu ) 여성건강간호학회 2015 여성건강간호학회지 Vol.21 No.4
This study was aimed to understand the meaning and essentials of the experience of burnout for hospital nurses with turnover intention. Methods: The design was a qualitative research of phenomenological study. Participants: Seven hospital nurses who had worked over three years and had experiences of turnover intention in a hospital with over 400 beds were included. Results: Nine meaningful themes related to burnout experiences and four theme clusters of 1) battery warning sounds almost out; 2) the player who hit the drum and double-headed drum; 3) the target flying arrow without a break; and 4) the pendulum swaying to turn over. Registered nurses (RNs) felt burnout with an overload of work and by the thought that it was illegal action for registered nurses to receive insufficient rewards for their work. RNs also experienced there were no problem solving strategies to verbal violence by patient and medical team. Conclusion: The findings show that burnout experiences for those who had turnover intention was developed from the insight that insufficient training to do work independently with over-load for nurses was not ethical. It suggests that it is necessary to rethink training systems for nursing and hospitals to relieve turnover intention.
Studies on Biological Effects of Gamma-ray in Sorghum bicolor M.
Seung Bin Im,Jaihyunk Ryu,Yu-Mi Lee,Soon-Jae Kwon,Dong Sub Kim,Jin-Baek Kim,Sang Hoon Kim,Hee-bong Lee,Si-Yong Kang 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
This study was conducted to determine the optimal dose of gamma-ray for mutation breeding in sorghum (Sorghum bicolor M.). Gamma-rays irradiated to dry seeds with various doses (0 to 1000 Gy) at Korea Atomic Energy Research Institute. Lethal dosage (LD50) was approximately 256 Gy. Significant decreases in growth characteristics (plant height, tiller number and fresh weight) were observed by dose of increased over 300 Gy. Reduction doses (RD50) was approximately 363 Gy in plant height. We also conducted comet assay to observe nucleus DNA damage due to gamma irradiation. In comet assay, a clear difference was identified over 100 Gy treatments. With increasing doses of gamma-ray in the range of 100 to 400 Gy, the rate of head DNA was decreased significantly from 93.52% to 67.57%. The most of the Sorghum cells were severely damaged in the integrity of DNA by gamma-ray. These data provide valuable information when the optimal dose should be chosen for purpose of mutation breeding program of Sorghum.