Effect of ER signal peptides (KDEL) on expression and function of anti-virus monoclonal antibody in plant

Da-Young Park,Yangkang So,Kyung-Jin Lee,Zhe Lu,Eun-Yi Oh,Kwang-Wok Min,Seul-Ki Lee,Jung-Hwan Lee,Mi-Hyun Ahn,Mira Song,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering has led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current antibody expression systems. In this study, the expression levels of mAbP SO57 with or without ER-signal peptides (Lys-Asp-Gly-Leu;KDEL) in transgenic tobacco plants were analysed in transgenic plant. PCR and Reverse Transcription-PCR analyses showed existence of heavy and light chain genes of mAb with or without KEDL and their transcription in plant, respectively. Western blot showed that the expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb have similar binding activity to the FcγRI receptor. High-performance liquid chromatography analysis showed that the mAbP SO57 with KDEL had glycan profile with both oligomannose and golgi type, whereas the mAbP SO57 without KDEL had only golgi type glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest that the potential of mAbP SO57 for rabies immunotherapy is regardless of plant specific glycan structures.

林地利用에 따른 溪流水의 水質變化

具瀟瑛,鄭源玉,金弘洙,朴晋源,麻鎬燮 경상대학교 농과대학 부속연습림 2001 演習林硏究報告 Vol.- No.11

Table 2. Evaluation of stream water quality in three stands by Drinking water quality standard <원문참조> 임지이용에 따른 수질변화 및 오염정도를 구명하여 향후 비점오염원 관리를 위한 기초자료를 제공하고자 경남 진주시 정평리에 위치한 3개 임분(소나무, 밤나무Ⅰ, 반나무Ⅱ임분)에서 2000년 7월부터 9월까지 계류수의 수질변화를 분석하여 얻은 결과는 다음과 같다. 1. 세 임분의 계류수 pH는 소나무 임분(pH6.59)>밤나무 Ⅱ임분(ph 6.53)>밤나무 Ⅰ임분(pH 6.47)계류수 순으로 나타났다. 2. 전기전도도는 밤나무 Ⅰ임분>소나무 임분>밤나무 Ⅱ임분 계류수의 순으로 높게 나타났다. 3. 양이온은 세 임분의 계류수 모두 Ca^2+, Na^+, Mg^2+, K^+, NH_^+의 순으로 높게 나타났다. 4. 음이온은 소나무 임분과 밤나무 Ⅱ임분 계류수에서는 SO_4^2, Cl, NO_3의 순으로 높게 나타났고, 밤나무 Ⅰ임분 계류수에서는 NO_3, SO_4^2, Cl 의 순으로 높게 나타나 차이를 보였다. 5. 소나무, 밤나무 Ⅱ임분 계류수는 pH, NH_4+, NO_3, Cl, SO_4^2이 먹는 물 수질기준 범위내에 있었다. 그러나 밤나무 Ⅰ임분 계류수에서 5항목 중 NO_3는 먹는 물 수질기준을 넘어서는 범위에 있어 임지이용에 따른 하류수질의 오염이 우려되었다. 따라서 이러한 상류지역에서 오염원 차단을 위한 수질정화시설 등의 대책이 마련되어야 할 것으로 사료된다. This study was carried out to clarify the change characteristics of stream water quality by land-use of forest from July to September, 2000 in three stands(Pinus densiflora, Castanea crenata(Ⅰ), Castanea crenata(Ⅱ) stand) of Jeongpyeong-ri, Jinju-si, Gyeongnam. The mean pH of rainfall results in acid rain of 5.3. The pH of stream water in three stands was hugh in order of Pinus densiflora (pH 6.59), Castanea crenata(Ⅱ)(pH 6.53) and Castanea crenata(Ⅰ) stand(pH 6.47). The electrical conductivity of stream water was high in order of Castanea crenata(Ⅰ), Pinus densiflora and Castanea crenata(Ⅱ) stand. Cations of three stand in stream water high in order of Ca^2+, Na^+, Mg^2+, K^+ and NH_4^+. But anions of stream water in Pinus densiflora stand and Castanea crenata(Ⅱ) stand were high in order of SO_4^2, Cl and NO_3. In Castanea crenata(Ⅰ) stand cations of stream water were high in order of NO_3, SO_^2 and Cl. The level of pH, NH_4^+, NO_3, Cl and SO_4^2 of stream water in Pinus densiflora stand and Castanea crenata(Ⅱ) stand reached within the level of domestic use standard for drinking water But the level of NO_3 of stream water in Castanea crenata(Ⅰ) stand was higher than that of domestic use standard. Therefore, non-point sources like forest watersheds which are fertilizer application lands should be taken to the appropriate mitigation measures.

Effect of ER signal peptides (KDEL) on expression and function of monoclonal antibody in plant

Da-Young Park,Eun-Yi Oh,Zhe Lu,Yangkang So,Mi-Hyun Ahn,Mira Song,Kyung-Jin Lee,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current animal expression systems. In this study, the expression level of mAbP SO57 between with and without Lys-Asp-Glu-Leu (KDEL) was compared in transgenic plant. PCR and PT-PCR analyse showed stable gene insertion transcription of heavy and light chain genes of mAbP SO57 with or without KDEL in plant, respectively. We validated expression of mAbP SO57 by western blot. Western blot showed the significantly higher expression level of mAbP SO57 with KDEL compared to without KDEL. Flow cytometry (FACS) analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb (mAbM) evidenced similar binding activity to the FcγRI receptor (CD64). High performance liquid chromatography (HPLC) analysed, glycosylation patterns of mAbP SO57 with or without KDEL. The mAbP SO57 with KDEL had glycan profile with both oligomannose type (47.6%) and golgi type (52.4%), while the mAbP SO57 without KDEL had only golgi type (100%) glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest the potential of mAbP SO57 for rabies immunotherapy, regardless of plant specific glycan structures.

SOD2와 NDPK2 유전자 집적에 의한 페튜니아의 아황산가스 저항성 증진

이수영(Su Young Lee),천경성(Kyeong-Seong Cheon),김소영(So Young Kim),권오현(O Hyeon Kwon),이혜진(Hye Jin Lee),김원희(Won Hee Kim),유봉식(Bong Sik Yoo) 한국원예학회 2016 원예과학기술지 Vol.34 No.1

유전자 집적에 의해 아황산가스 저항성 증진 페튜니아를 개발하고자 비생물적스트레스 저항성 유전자로 널리 알려진 SOD2 와 NDPK2유전자가 각각 도입된 SOD2 형질전환 계통 SOD2-2-1-1-35(T₄)[S(T₄)]와 NDPK2 형질전환 계통 NDPK2-7-1(T₂)[N7-1(T₂)]간 상호교잡을 실시하여 교잡 제1세대를 획득하였다. 교잡 제1세대 중 SOD2와 NDPK2유전자가 모두 집적된 개체 획득율은 32.1-73.0%이었고, SOD2와 NDPK2유전자가 모두 집적된 개체는 SOD2 또는 NDPK2유전자가 단독 도입된 개체에 비해 아황산가스 30ppm처리 피해율이 2.6-5.1배 낮아 아황산가스 저항성이 증진되었음을 확인하였다. 또한 아황산가스 저항성이 증진된 교잡 제1세대를 자가수분에 의해 세대진전 시켜 획득한 교잡 제2세대의 아황산가스 저항성 검정을 통하여 교잡 제1세대에서의 아황산가스 저항성이 후대에서도 안정적으로 발현함을 확인하였다. 또한, 광합성 효율 증진 조사를 통해 아황산가스 저항성이 증진된 SOD2와 NDPK2유전자 집적 후대가 아황산 가스에 대한 스트레스를 덜 받고 있음을 확인하였고, RT-qPCR분석을 통해 SOD2와 NDPK2유전자 집적 후대의 아황산가스 저항성 증진이 SOD2와 NDPK2 유전자 집적에 의한 효과임을 확인하였다. The aim of this study was to develop a transgenic petunia with enhanced resistance to sulfur dioxide (SO₂) gas by stacking two genes, SOD2 and NDPK2, which are both known to confer resistance to abiotic stresses. The first-generation hybrids (TF1) were obtained through reciprocal crosses between an SOD2-transgenic line SOD2-2-1-1-35(T₄)[S(T₄)] and an NDPK2- transgenic line NDPK2-7-1(T₂)[N7-1(T₂)]. Approximately 32.1-73.0% of the first-generation hybrids (TF₁) carried both SOD2 and NDPK2 genes. These hybrids showed 2.6 and 5.1 times less damage than hybrids carrying only SOD2 or NDPK2 genes, respectively, when they were treated with SO₂ gas at 30 ppm. This confirmed that the heterozygous hybrids were more resistant to SO₂ than the hybrids carrying either one of the resistance genes. Second-generation hybrids (TF₂) were obtained by selfing the TF₁ individuals. We confirmed the expression of the stacked genes in the TF₂ hybrids by phenotypic observation of their response to SO₂ gas at 30 ppm as well as using RT-qPCR and photosynthetic efficiency.

콘크리트 중력식댐의 내진성능 평가에 관한 연구

소진호,정영수 중앙대학교 건설환경연구소 2001 環境科學硏究 Vol.12 No.1

The objective of this study is firstly to frame up the seismic safety of concrete gravity dams. It is necessary to analyze seismic response and evaluate seismic performance off concrete gravity dams during earthquake. In this study, seismic damage and dynamic analysis of concrete gravity dams using SAP2000 program are performed. Additional dynamic water pressure due to earthquake was considered as additional mass for numerical seismic analysis. But, a further research will be necessary for the seismic stability of dams.

Complete nucleotide sequence of the penicillin G acylase gene and the flanking regions, and its expression in Escherichia coli

오상진, 김영창, 박영우, 민소영, 김인숙, 강현삼 忠北大學校 遺傳工學硏究所 1988 遺傳工學硏究 Vol.2 No.1

본문참고

서아프리카의 이슬람 무장단체에 관한 소고 : GSIM을 중심으로

진소영 부산외국어대학교 아프리카연구센터 2021 아프리카학 연구 Vol.1 No.1

Since 2017, Islam terrorist threats are increasing in the Sahel region, including Niger and Burkina Faso, mainly in Mali, Africa. The cause is related to the Groupe de soutien à l’islam et aux musulmans(GSIM). GSIM is a merger of four terrorist groups operating in North and West Africa in 2017. The GSIM consists of four terrorist organizations; Al-Qaeda in the Islamic Maghreb, Al-Mourabitoun, Ansar Dine, Macina Liberation Front. All four terrorist groups have pledged in line with Al-Qaeda, and GSIM is also an Al-Qaeda affiliate. GSIM’s leader is Iyad ag ghali, the leader of Ansar Dean. GSIM become the largest terrorist group in West Africa with the coalition of Islamic militants. The fighters who make up GSIM have various nationalities and tribes. Also, GSIM is associated with group cause complications between local ethnic groups in Mali, particularly the conflict between the Fulani fighters on the Macina Liberation Front and Dogon people in Mali. GSIM recognize each terrorist group’s authority over its own occupied territories and share and operate information by exchanging tactics with each other. Despite the Covid 19 situation, the security of the Sahel region is at risk as the GSIM’s increasing steps day by day. GSIM’s act of terrorism is not just the ideology of religion. This is the result of a combination of many cause, including climate change, food shortages, and etgnic conflicts. Therefore, it is necessary to bring peace in West Africa not only from a religious point of view but also from a variety of social perspectives. [Key words: GSIM, Armed Islamic Group of Algeria, Sahel, West Africa, North Africa]

외과적 정출술을 통한 치은 하방 치경부 파절선이 있는 상악 전치부의 심미 보철 치료

이소진,김유진,박영범,조규성,정문규 大韓齒科補綴學會 2012 대한치과보철학회지 Vol.50 No.3

Surgical extrusion, immediate extrusion following tooth luxation, is a method to preserve one's natural tooth and achieve esthetic restoration without additional periodontal surgery when subgingival dental caries or crown fracture occurs. A 16-year-old male was referred to the clinic from the department of operative dentistry for the esthetic restoration of maxillary left lateral incisor. Due to the crown to root fracture, the tooth was endodontically treated with a buccal crown length of 4 mm. When the palatal flap was elevated, the mesiopalatal cervical fracture area was situated 3 - 4 mm subgingivally. Crown lengthening was achieved through surgical extrusion. After 3 months of clinical observation and provisional restoration, the maxillary left central incisor was restored with all ceramic crown and obtained a satisfactory clinical result. 치은연 하방에 치아 우식증이나 치아 파절이 발생했을 때, 치아를 탈구시켜 즉시 정출시키는 외과적 정출술을 이용한 치관 연장술은 자연치를 보존하고 추가적인 치주적 수술 없이 단기간에 심미적인 보철 치료를 가능하게 한다. 본 환자는 16세 남환으로 본원 보존과로부터 상악 좌측 중절치의 심미 수복을 위해 본원 보철과로 의뢰되었다. 상악 좌측 중절치는 근관치료가 되어 있었으며 치관-치근 파절로 인해 협측 치관 길이는 4 mm였으며 구개측 판막을 열었을 때 근심 구개측 치경부 파절 범위는 치은 하방 3 - 4 mm에 위치하였다. 외과적 정출술을 통한 치관연장술을 시행하였으며 경과 관찰 및 임시 치아의 단계를 거쳐 3개월 후에 전부 도재관으로 수복하여 만족할 만한 임상 결과를 얻을 수 있었다.

한의과대학 부속 한방병원 한방응급실 내원환자에 대한 임상적 분석

소형진,류재환,김영철,이범준 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2007 東西醫學硏究所 論文集 Vol.2007 No.-

Objectives : By recording basic data of patients visiting the emergency room of the oriental medicine center, we can understand their characteristics and gain better insight about them. Methods : We performed a retrospective study of 2,674 patients who visited the emergency room of Kyunghee Oriental Medical Center, from June 1, 2006 to May 31, 2007. Results : 1. Of 2,674 total patients, the male to female ratio was 0.94:1 and the 6th decade of life was the peak age group. 2. The majorit of patients visited our emergency room between 06:00 and 24:00(midnight). 3. Most patients visited on a Saturday or Sunday. 4. By monthly distribution, the patients incrcased slightly during June, July, August and October. 5. The majority of patients visited the emergency room within 24 hours of onset. 6. The patients were categorized as follows : neurological diseases 71.15%, musculoskeletal diseases 16.02%, internal medical discase 9.04% psychiatric and related disease 3.79%. 7. The average transit time waS about 1 hour 30 minutes. 8. Admission rate was 48% and the rate of admission to the intensive care unit (ICU) was 5% of total visiting patients. Conclusion : Assuming that this research will continue adding patien's data continuously, this work will help us to understand the features of patients who visiting the emergency room of the oriental medical center.

위선종 및 조기 위암종과 동반된 Helicobacter pylori 위염에서 세포고사 지수, Ki-67 증식지수 및 p53 단백 발현

진소영,이동화 순천향의학연구소 2002 Journal of Soonchunhyang Medical Science Vol.8 No.1

Helicobacter pylori(H. pylori) is the main cause of chronic gastritis, which may progress to cancer through atrophy, metaplasia, and dysplasia. To investigate the relationship between H. pylori and gastric precancerous and cancerous lesions, we examined a total of 55 cases of H. pylori gastritis associated with 30 gastric adenomas and 25 early gastric cancers. Apoptosis index, Ki-67 proliferation index, and p53 protein expression were counted after TUNEL and immunohistochemistry. Apoptosis indices of each group were statistically not significantly different. Ki-67 proliferation index was highest in adenoma group (15.1±8.3) and lowest in submucosal carinoma group (7.8±8.3)(p<0.05). Overall p53 protein expression rate was 49.1%. The pattern of p53 protein expression was scattered in adenoma group(5/6) whereas more grouped in higher grade lesion group. According to the above findings, relative decrement of apoptosis and grouped pattern of p53 protein expression in H. pylori gastritis associated with higher grade lesion might be related with progression of H. pylori gastritis to the precancerous and cancerous lesions.