전기화학적 부식측정기법을 이용한 철근 콘크리트의 부식특성평가

이수열(Su Youl Yi),이재봉(Jae Bong Lee) 한국부식방식학회 2000 Corrosion Science and Technology Vol.29 No.6

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JS-III-49, a hydroquinone derivative, exerts anti-inflammatory activity by targeting Akt and p38

Yi, Young-Su,Kim, Mi-Yeon,Cho, Jae Youl The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.3

Since previous studies have reported that hydroquinone (HQ) exerted immunosuppressive and anti-inflammatory activity, various HQ derivatives have been synthesized and their biological activities investigated. In this study, we explored the anti-inflammatory activity of JS-III-49, a novel HQ derivative, in macrophage-mediated inflammatory responses. JS-III-49 suppressed the production of the inflammatory mediators nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) and down-regulated the mRNA expression of the inflammatory enzymes cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) as well as the expression of the pro-inflammatory cytokines interleukin-6 (IL-6) and IL-$1{\beta}$ without cytotoxicity in LPS-stimulated RAW264.7 cells. JS-III-49 inhibited nuclear translocation of the $NF-{\kappa}B$ transcription factors p65 and p50 by directly targeting Akt, an upstream kinase of the $NF-{\kappa}B$ pathway, in LPS-stimulated RAW264.7 cells. However, JS-III-49 did not directly inhibit the kinase activities of Src and Syk, which are upstream kinases of Akt, in LPS-stimulated RAW264.7 cells. Moreover, JS-III-49 suppressed the nuclear translocation of c-Fos, one of the components of AP-1, by specifically targeting p38, an upstream mitogen-activated protein kinase (MAPK) in the AP-1 pathway in LPS-stimulated RAW264.7 cells. These results suggest that JS-III-49 plays an anti-inflammatory role in LPS-stimulated macrophages by targeting Akt and p38 in the $NF-{\kappa}B$ and AP-1 pathways, respectively.

Benzothiadiazole-elicited defense priming and systemic acquired resistance against bacterial and viral pathogens of pepper under field conditions

Hwe-Su Yi,Jung Wook Yang,Hye Kyung Choi,SA-YOUL GHIM,류충민 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.4

Like the innate immunity in mammals, plants have developed an induced resistance, referred to as systemic acquired resistance (SAR). Recently, defense priming that is not related to the direct activation of defenses,but instead elicits more rapid induction of resistance mechanisms following trigger application, has been proposed to explain the long-lasting effect of SAR. However,the majority of previous studies have focused on understanding the molecular mechanism underlying priming under in vitro and laboratory conditions. This study examined whether defense priming occurred and was detectable with SAR marker genes by a chemical elicitor,benzothiadiazole (BTH), under field conditions. Pepper seedling application of 0.5 mM BTH was sufficient to prime the CaPR4 gene for 20 days as well as to induce SAR against bacterial spot caused by Xanthomonas axonopodis. Transcriptome analysis revealed to prime defense hormonal signaling and antimicrobial compound production genes. At the end of the season, when bacterial spot and Cucumber mosaic virus disease outbreaks naturally occurred, BTH-treated plants demonstrated less disease symptoms. Our results indicate that the priming of SAR genes plays a critical role in plant protection against pathogens under natural conditions.

JS-III-49, a hydroquinone derivative, exerts anti-inflammatory activity by targeting Akt and p38

Young-Su Yi,Mi-Yeon Kim,Jae Youl Cho 대한생리학회-대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.3

Since previous studies have reported that hydroquinone (HQ) exerted immunosuppressive and anti-inflammatory activity, various HQ derivatives have been synthesized and their biological activities investigated. In this study, we explored the anti-inflammatory activity of JS-III-49, a novel HQ derivative, in macrophagemediated inflammatory responses. JS-III-49 suppressed the production of the inflammatory mediators nitric oxide (NO) and prostaglandin E₂ (PGE₂) and downregulated the mRNA expression of the inflammatory enzymes cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) as well as the expression of the pro-inflammatory cytokines interleukin-6 (IL-6) and IL-1β without cytotoxicity in LPS-stimulated RAW264.7 cells. JS-III-49 inhibited nuclear translocation of the NF-κB transcription factors p65 and p50 by directly targeting Akt, an upstream kinase of theNF-κB pathway, in LPS-stimulated RAW264.7 cells. However, JS-III-49 did not directly inhibit the kinase activities of Src and Syk, which are upstream kinases of Akt, in LPSstimulatedRAW264.7 cells. Moreover, JS-III-49 suppressed the nuclear translocation of c-Fos, one of the components of AP-1, by specifically targeting p38, an upstream mitogen-activated protein kinase (MAPK) in the AP-1 pathway in LPS-stimulated RAW264.7 cells. These results suggest that JS-III-49 plays an anti-inflammatory role in LPS-stimulated macrophages by targeting Akt and p38 in the NF-κB and AP-1 pathways, respectively.

Antifungal activity of nitrogen-fixing bacteria isolated from rice roots

Lee Su-Jin,Jeong Da-eun,Jeoung Ji-hyun,Yi Hwe-Su,Seul Keyung-Jo,Ghim Sa-Youl 한국생명과학회 2006 한국생명과학회 심포지움 Vol.46 No.-

Ethnopharmacological properties of <i>Artemisia asiatica</i>: A comprehensive review

Ahuja, Akash,Yi, Young-Su,Kim, Mi-Yeon,Cho, Jae Youl Elsevier 2018 Journal of Ethnopharmacology Vol.220 No.-

<P><B>Abstract</B></P> <P><B>Ethnopharmacological relevance</B></P> <P> <I>Artemisia asiatica</I> Nakai (Compositae) has a long history as a traditional remedy. Preparation from various parts of the plant (aerial parts and leaves) are used to treat a wide range of diseases including gastric trouble, liver dysfunction, and skin inflammation.</P> <P><B>Aims of this review</B></P> <P>The aims of this review were: 1) to provide an overview of recent studies and progress on <I>A. asiatica</I>-derived ethnopharmacological compounds and their pharmacological activities; and 2) to summarize existing evidence and provide insight for future studies.</P> <P><B>Materials and methods</B></P> <P>This investigation was carried out by analyzing published books and research papers <I>via</I> scientific databases, namely Science Direct, PubMed ACS Publication, Wiley Online Library, CNKI and information obtained online. The keywords “<I>Artemisia asiatica</I> traditional uses,” “Compounds isolated and studied in <I>Artemisia asiatica,</I>” and “Pharmacological advances in <I>Artemisia asiatica</I>” were used and articles published between 1995 and 2017 were considered. In total, 500 works related to biological activities of <I>A. asiatica</I> were identified, and only materials published in English were included in the review.</P> <P><B>Results</B></P> <P>Comparative analysis of literature searched through sources available confirmed that the ethnopharmacological use of <I>A. asiatica</I> was recorded in Korea, China, and Japan. Phytochemical studies revealed the presence of flavonoids, sesquiterpene lactones, monoterpenes, and steroids in <I>A. asiatica</I>. Of these, flavonoids have been shown to exhibit significant pharmacological effects such as gastroprotective, anti-inflammatory, anti-tumor, and anti-microbial actions.</P> <P><B>Conclusions</B></P> <P>Phytochemical and pharmacological studies of <I>Artemisia asiatica</I> have proven that this plant is one of valuable medicinal sources with neuroprotective, gastroprotective, anti-oxidative, anti-inflammatory, and anti-cancer effects. Although ethanol extract of this plant is now being prescribed as gastroprotective and anti-ulcerative medicine, it is now time to expand its application to other human inflammatory diseases such as pancreatitis and hepatitis and further extensive study on toxicity in human. Therefore, the present review will encourage further studies of <I>A. asiatica</I> in the pursuit of wide range of therapeutic remedy.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Alcohol-induced Hyperlipidemia Is Ameliorated by Orally Administered DWP208, a Sodium Succinate Form of ZYM201

Cho, Jae Youl,Choi, Jongwon,Park, Jae Gwang,Yi, Young-Su,Hossen, Muhammad Jahangir,Kim, Hyeongmin,Ro, Jieun,Cha, Bae Cheon,Yoo, Eun Sook,Kim, Jong-Hoon,Lee, Jaehwi The Korean Society of Pharmacology 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.6

DWP208 is a sodium succinate form of ZYM-201 which is a triterpenoid glycoside isolated from Sanguisorba officinalis, a medicinal plant prescribed for various diseases, such as duodenal ulcers and bleeding in East Asian counties. We demonstrated that this compound is able to normalize the altered lipid metabolism induced by hyperglycemia and a high fat diet. In this study, we determined whether hyperlipidemic conditions induced with chronically treated alcohol can also be restored by DWP208. Similar to our previous results, orally administered DWP208 (1 to 10 mg/kg) also ameliorated the hyperlipidemia that was induced by alcohol. This compound reversed the alcohol-induced hyperlipidemia including (i) up-regulated hyperlipidemic parameters such as low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), atherosclerotic index (AI), triglyceride, and total cholesterol, and (ii) down-regulated hyperlipidemic parameters such as absolute body weight, superoxide dismutase (SOD) activity, and high-density lipoprotein (HDL) in serum and liver. According to our data, the ameliorative activity of DWP208 is due to its indirect anti-oxidative activity as a result of which lipid peroxide and hydroxyl radical levels were reduced and the activity of SOD was enhanced. Therefore, our data strongly suggest that DWP208 can be used as a remedy against alcohol-induced hyperlipidemia.

8-(Tosylamino)quinoline inhibits tumour progression through targeting phosphoinositide-3-kinase/Akt pathway.

Jung, Yongwoo,Yi, Young-Su,Yoo, Dae Sung,Kim, Ji Hye,Yang, Woo Seok,Lee, Jongsung,Park, Kye Won,Kweon, Dae-Hyuk,Hong, Sungyoul,Cho, Jae Youl Govi-Verlag Pharmazeutischer Verlag [etc.] ; Verla 2013 PHARMAZIE Vol.68 No.2

<P>We examined whether 8-(tosylamino)quinoline (8-TQ), a structural analogue of BAY 11-7082, is able to modulate various tumourigenic responses using various in vitro and in vivo experimental conditions. 8-TQ exhibited the strongest suppressive activity on the proliferation of C6, A431, HeLa and MDA-MB-231 cells with IC550 values ranging from 10 to 30 microM. According to the analysis of level of active caspase-3, and morphologies of C6, HeLa and MDA-MB-231 cells, it was revealed that 8-TQ is able to induce apoptosis. Furthermore, this compound strongly diminished the invasion of MDA-MB-231 cells, the migration of HeLa cells, and the new generation of blood vessels under non-toxic conditions. Reduction of the phospho-form levels of intracellular signalling enzymes by 8-TQ strongly indicated that molecular signalling machineries composed of phosphoinositide 3-kinase (PI3K)/phosphoinositide-dependent kinase-1 (PDK1)/Akt and extracellular-signal-regulated kinase (ERK) could be targeted by 8-TQ treatment. Indeed, the specific inhibitors (LY294002 and U0126) of PI3K/PDK1/Akt and ERK showed similar anti-cancer properties to 8-TQ. Finally, 8-TQ intraperitoneally injected suppressed the increase of tumour volume up to 40% compared to vehicle-treated control. Taken together, our results clearly suggest that 8-TQ might have applications as a novel anti-cancer drug or may be served as a lead compound to be further optimized.</P>

Ni 및 Cu무전해 도금법에 의해 제조한 $LaNi_5$ 전극의 전기화학적 특성

이수열,이재봉,Yi Su Youl,Lee Jae-Bong 한국전기화학회 2000 한국전기화학회지 Vol.3 No.2

[ $AB_5$ ] 수소저장합금인 $LaNi_5$, 합금분말에 Ni 및 Cu 무전해 도금의 영향을 전기 화학적 실험을 통하여 고찰하였다. 전기 화학적 실험은 정전류 충$\cdot$방전 실험, 순환전류전위 실험, 교류 임피던스 실험 등을 실시하여 도금하지 않은 $LaNi_5$ 전극과 Ni 및 Cu 무전해 도금한 전극간의 특성을 비교 연구하였다. 현상학적인 분석으로는 SEM을 이용하여 분말상의 미세조직을 관찰하였으며 X-선 회절시험을 실시하였다 무전해 도금을 실시하여 Ni 및 Cu박막이 피복된 수소저장 합금은 활성화 특성파 싸이클 수명 등의 특성이 개선되었으며 도금하지 않은 전극에 비하여 반응속도가 증가하였다. 또한 충$\cdot$방전이 반복됨에 따라 전극과 전해질 계면에서의 전하이동저항이 현저하게 감소하였다. 따라서 본 연구에서 실시한 $LaNi_5$, 활물질에 Ni및 Cu 무전해 도금을 실시하면 초기 활성화반응을 촉진시키며 $LaNi_5$활물질이 전해질과의 직접 접촉을 피하게 되어 전극의 수명을 증가시키는 것을 알 수 있었다. The effect of electroless Ni and Cu plating on $LaNi_5$, $AB_5$ type hydrogen storage alloy was investigated by the various electrochemical techniques such as constant current charge-discharge test, cyclic voltammeoy, and a.c. impedance spectroscopy. Scanning electron microscopy and X-ray diffraction test were conducted for phenomenological logical analyses. Cyclic Voltammetry results show that activation characteristics, cycle life and reaction ,rate were improved through electroless Ni and Cu plating. Compared with bare $LaNi_5$ the charge transfer resistance of electrode was greatly reduced as charge-discharge cycle increases. Therefore, electroless Ni and Cu plating on $LaNi_5$ alloy tends to accelerate the early activation, increasing the cyclic lift of electrode.

Effect of polysaccharides from a Korean ginseng berry on the immunosenescence of aged mice

Kim, Miseon,Yi, Young-Su,Kim, Juewon,Han, Sang Yun,Kim, Su Hwan,Seo, Dae Bang,Cho, Jae Youl,Shin, Song Seok The Korean Society of Ginseng 2018 Journal of Ginseng Research Vol.42 No.4

Background: Korean ginseng has been widely evaluated to treat human diseases; however, most studies on Korean ginseng have focused on its root. In this study, polysaccharides [acidic-polysaccharide-linked glycopeptide (APGP) extracted with 90% ethanol and hot water] were prepared from Korean ginseng berries, and their effect on immunosenescence was explored. Methods: The effect of APGP on thymic involution was evaluated by measuring the size of thymi dissected from aged mice. The effect of APGP on populations of immune cells, including natural killer (NK) cells, dendritic cells, age-correlated CD11c-positive B cells, and several subtypes of T cells [CD4-positive, CD8-positive, and regulatory (Treg) T cells] in the thymi and spleens of aged mice was analyzed by fluorescence-activated cell sorting analysis. Serum levels of interleukin (IL)-2 and IL-6 were evaluated by enzyme-linked immunosorbent assay analysis. Profiles of APGP components were evaluated by high-performance liquid chromatography (HPLC) analysis. Results: APGP suppressed thymic involution by increasing the weight and areas of thymi in aged mice. APGP increased the population of NK cells, but showed no effect on the population of dendritic cells in the thymi and spleens of aged mice. APGP decreased the population of age-correlated CD11c-positive B cells in the spleens of aged mice. APGP showed no effect on the populations of CD4- and CD8-positive T cells in the thymi of aged mice, whereas it increased the population of Treg cells in the spleens of aged mice. APGP further decreased the reduced serum levels of IL-2 in aged mice, but serum levels of IL-6 were not statistically changed by APGP in aged mice. Finally, HPLC analysis showed that APGP had one major peak at 15 min (a main type of polysaccharide) and a long tail up to 35 min (a mixture of a variety of types of polysaccharides). Conclusion: These results suggested that APGP exerted an anti-immunosenescent effect by suppressing thymic involution and modulating several types of immune cells.