Etiology and clinical characteristics of fever of unknown origin in children: a 15-year experience in a single center

Kim, Yi-Seul,Kim, Kyung-Ran,Kang, Ji-Man,Kim, Jong-Min,Kim, Yae-Jean The Korean Pediatric Society 2017 Clinical and Experimental Pediatrics (CEP) Vol.60 No.3

Purpose: Fever is one of the most common symptoms in children. In previous studies, infectious disease was the most common cause of pediatric fever of unknown origin (FUO). The aim of this study is to investigate the etiology, clinical characteristics and prognosis of pediatric FUO in 21 century with more diagnostics available and to analyze the factors for certain disease categories. Methods: Among the children under 18 years old who were hospitalized at Samsung Medical Center from January 2000 to December 2014, the patients who met the criteria including fever of ${\geq}38.0^{\circ}C$ for longer than ${\geq}14days$ and failure to reach a diagnosis after one week of investigations were included. Results: Total 100 patients were identified. Confirmed diagnosis was achieved in 57 patients (57%). Among them, infectious diseases (n=19, 19%) were most common, followed by connective tissue diseases (n=15, 15%), necrotizing lymphadenitis (n=8, 8%), and malignancies (n=7, 7%). Children with fever duration over 28 days had a trend for higher frequency of connective tissue diseases (28.3%) except undiagnosed etiology. The symptoms such as arthritis, lymph node enlargement and only fever without other symptoms were significantly related with connective tissue diseases, necrotizing lymphadenitis and undiagnosed respectively (P<0.001). Ninety-two patients have become afebrile at discharge and 1 patient died (1%). Conclusion: Almost half of our patients were left without diagnosis. Although it has been known that infectious disease was most common cause of pediatric FUO in the past, undiagnosed portion of FUO have now increased due to development of diagnostic techniques for infectious diseases.

Role of the epithelial–mesenchymal transition and its effects on embryonic stem cells

Kim, Ye-Seul,Yi, Bo-Rim,Kim, Nam-Hyung,Choi, Kyung-Chul Nature Publishing Group 2014 Experimental and molecular medicine Vol.46 No.8

<P>The epithelial–mesenchymal transition (EMT) is important for embryonic development and the formation of various tissues or organs. However, EMT dysfunction in normal cells leads to diseases, such as cancer or fibrosis. During the EMT, epithelial cells are converted into more invasive and active mesenchymal cells. E-box-binding proteins, including Snail, ZEB and helix–loop–helix family members, serve as EMT-activating transcription factors. These transcription factors repress the expression of epithelial markers, for example, E-cadherin, rearrange the cytoskeleton and promote the expression of mesenchymal markers, such as vimentin, fibronectin and other EMT-activating transcription factors. Signaling pathways that induce EMT, including transforming growth factor-β, Wnt/glycogen synthase kinase-3β, Notch and receptor tyrosine kinase signaling pathways, interact with each other for the regulation of this process. Although the mechanism(s) underlying EMT in cancer or embryonic development have been identified, the mechanism(s) in embryonic stem cells (ESCs) remain unclear. In this review, we describe the underlying mechanisms of important EMT factors, indicating a precise role for EMT in ESCs, and characterize the relationship between EMT and ESCs.</P>

Origin-related study of genetic diversity and heteroplasmy of Mongolian sheep (Ovis aries) using mitochondrial DNA

Yi Seul Kim,Khaliunaa Tseveen,Badamsuren Batsukh,Jiyeon Seong,Hong Sik Kong 한국동물생명공학회(구 한국동물번식학회) 2020 Journal of Animal Reproduction and Biotechnology Vol.35 No.2

Food and agricultural production sector, especially livestock production is vital for Mongolia’s economic and social development. Domestic sheep play key roles for Mongolians, providing food (meat, milk) and raw materials (wool, sheepskin), but genetic diversity, origin of sheep populations in Mongolia have not been well studied. Studies of population genetic diversity is important research field in conservation and restoration of animal breeds and genetic resources. Therefore, this study aimed to investigate genetic characteristics and estimate origin through the analysis of mitochondrial DNA control region D-loop and Cytochrome b of Mongolian indigenous sheep (Mongolian native, Orkhon and Altanbulag) and one Europe sheep (Suffolk). As a result of there were found, 220 SNPs (Single nucleotide polymorphism) in the D-loop region, 28 SNPs in the Cytochrome B region, furthermore, 77 Haplotypes. The nucleotide diversity was only found in D-loop region (n = 0.0184). Phylogenetic analysis showed that 3 (A, B, and C) of 5 haplogroups of sheep have been identified in our research. Haplogroup C was only found in Mongolian indigenous sheep. Haplogroup D and E were not observed. As a result of haplogroups, haplogroup A was dominant (n = 46 of 94 sheeps), followed by haplogroup B (n = 36) and haplogroup C (n = 12). Sequence analysis showed that T deletion, insertion and heteroplasmy in D-loop region occurred at a high rate in Mongolian indigenous sheep population (T insertion = 47, T deletion = 83). The heteroplasmy, which has never been found in Mongolian sheep, has been newly discovered in this study. As a result, the Mongolian sheep varieties, which mainly derived from Asia, were in hybridization with European sheep varieties.

Characerization of Cross-Linked Enzyme Aggregates of Acyl-CoA thioesterase1 precursor from Listeria innocus CLIP 11262

Seul Gi KIM,Song Yi BAE,Nam Yong KIM,So Hyun PARK,T. Doohun KIM 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

Analyses of Bacterial Communities in Meju, a Korean Traditional Fermented Soybean Bricks, by Cultivation-Based and Pyrosequencing Methods

Yi-Seul Kim,김민철,권순우,Soo-Jin Kim,In-Cheol Park,가종억,원항연 한국미생물학회 2011 The journal of microbiology Vol.49 No.3

Despite the importance of meju as a raw material used to make Korean soy sauce (ganjang) and soybean paste (doenjang), little is known about the bacterial diversity of Korean meju. In this study, the bacterial communities in meju were examined using both culture-dependent and independent methods in order to evaluate the diversity of the bacterial population. Analyses of the 16S rRNA gene sequences of the bacterial strains isolated from meju samples showed that the dominant species were related to members of the genera Bacillus, Enterococcus, and Pediococcus. The community DNAs extracted from nine different meju samples were analyzed by barcoded pyrosequencing method targeting of the V1 to V3 hypervariable regions of the 16S rRNA gene. In total, 132,374 sequences, with an average read length of 468 bp, were assigned to several phyla, with Firmicutes (93.6%) representing the predominant phylum, followed by Proteobacteria (4.5%) and Bacteroidetes (0.8%). Other phyla accounted for less than 1% of the total bacterial sequences. Most of the Firmicutes were Bacillus and lactic acid bacteria, mainly represented by members of the genera Enterococcus, Lactococcus, and Leuconostoc, whose ratio varied among different samples. In conclusion,this study indicated that the bacterial communities in meju were very diverse and a complex microbial consortium containing various microorganisms got involved in meju fermentation than we expected before.

Anti-Obesity Effect of Deodeok Vinegar on High Fat Diet Fed Rats

Yi-Seul Kim,In-Ho Kim,Seong-Ho Kim 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

PRMT7 deficiency causes dysregulation of the HCN channels in the CA1 pyramidal cells and impairment of social behaviors

Seul-Yi Lee,Tuan Anh Vuong,Hyun-Kyung So,Hyun-Ji Kim,Yoo Bin Kim,Jong-Sun Kang,Ilmin Kwon,Hana Cho 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

HCN channels regulate excitability and rhythmicity in the hippocampal CA1 pyramidal cells. Perturbation in the HCN channel current (Ih) is associated with neuropsychiatric disorders, such as autism spectrum disorders. Recently, protein arginine methyltransferase 7 (PRMT7) was shown to be highly expressed in the hippocampus, including the CA1 region. However, the physiological function of PRMT7 in the CA1 neurons and the relationship to psychiatric disorders are unclear. Here we showed that PRMT7 knockout (KO) mice exhibit hyperactivity and deficits in social interaction. The firing frequency of the CA1 neurons in the PRMT7 KO mice was significantly higher than that in the wild-type (WT) mice. Compared with the WT CA1 neurons, the PRMT7 KO CA1 neurons showed a more hyperpolarized resting potential and a higher input resistance, which were occluded by the Ih-current inhibitor ZD7288; these findings were consistent with the decreased Ih and suggested the contribution of Ih-channel dysfunction to the PRMT7 KO phenotypes. The HCN1 protein level was decreased in the CA1 region of the PRMT7 KO mice in conjunction with a decrease in the expression of Shank3, which encodes a core scaffolding protein for HCN channel proteins. A brief application of the PRMT7 inhibitor DS437 did not reproduce the phenotype of the PRMT7 KO neurons, further indicating that PRMT7 regulates Ih by controlling the channel number rather than the open probability. Moreover, shRNA-mediated PRMT7 suppression reduced both the mRNA and protein levels of SHANK3, implying that PRMT7 deficiency might be responsible for the decrease in the HCN protein levels by altering Shank3 expression. These findings reveal a key role for PRMT7 in the regulation of HCN channel density in the CA1 pyramidal cells that may be amenable to pharmacological intervention for neuropsychiatric disorders.

Anti-obesity effect of Burdock Vinegar on High Fat Diet Fed Rats

Yi-Seul Kim,In-Ho Kim,In-Ju Eom,Seong-Ho Kim 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Effects of e-health literacy and oral health knowledge on oral health behavior in adults

Yi Seul Kim,Soon Ryun Lim 한국치위생학회 2022 한국치위생학회지 Vol.22 No.1

Objectives: The purpose of this study was to identify new variables that can enhance adult oral health behaviors by confirming the degree of adult e-health literacy, oral health knowledge, and oral health behaviors and examining their relevance. Methods: A self-reported questionnaire was filled out by 350 adults from June 22 to August 1, 2021. Data were analyzed using SPSS 23.0. independent t-test, one way ANOVA, the scheffé post-hoc test and the pearson correlation coefficients were reviewed, A hierarchical regression analysis was conducted. Results: Oral health behaviors according to general characteristics showed significant differences in gender, educational background, dental visit within 1 year, subjective oral health status, oral health interest, frequency of oral internet use, and reliability of internet oral health information. Also it was found that e-health literacy affects oral health behavior. Conclusions: In this study, e-health literacy, oral health knowledge, and oral health behavior were correlated and it was confirmed that e-health literacy had an effect on oral health behavior. In the future, it is necessary to develop a tool that can measure e-oral health literacy and to find a way to improve the oral health behavior of adults by using e-oral health literacy.

Identification of two acetylcholinesterases in Drosophila suzukii

Yi Seul Kim,Young Ho Kim 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10

Most insects possess two different acetylcholinesterases (AChEs) loci (ace1 and ace2). In contrast, cyclorrhaphan flies,including Drosophila flies, are known to have only a single ace2 locus according to the previous studies. However, sequenceinformation of two different ace genes of Drosophila suzukii have been reported in GenBank. To identify two ace genes(Dsace-A and Dsace-B) in D. suzukii, therefore, we extracted total RNA from 6 individuals and two ace genes successfullywere amplified using gene-specific primers, suggesting that two Dsace genes are transcribed. According to the amino acidsequence analysis, DsAChE-A possesses typical amino acids for 3D structure and catalytic activity of AChE, while someamino acid residues were not conserved in DsAChE-B, suggesting that DsAChE-A and DsAChE-B may be respectivetrue gene and pseudogene of two ace in D. suzukii.