간 발생과정에 따른 Metallothionein 발현에 관한 연구

오승한,고정식,김완종 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

Metallothionein(MT) is a group of low molecular weight proteins, with a unique amino acid composition such as a large content (approximately 30%) of cysteine residues. The protein has been shown to play a physiological role in the absorption, transport, and metabolism, such as zinc, copper, and cadmium, but its actual functions are still not clear. This study was carried out to examine the expression of MT in developing or growing rat liver through immunohistochemical and RT-PCR method. Positive reactions against MT started to appear predominantly in the parenchmal cells of liver from the 13th day after gestation. Reaction products, or brown colorations, were localized at both the nucleus and the cytoplasm of the parenchymal cells. The intensity of this reaction gradually increased, and exhibited the strongest at birth. The intensity of MT staining diminished with growth, and by the 15th day after birth weak positive reaction was observed in the cells. In brief, positive reactions for MT were observed in the oval cells and the parenchymal cells during fetal stage, meanwhile they were present only in the parenchymal cells after birth. MT mRNA by RT-PCR seemed to be expressed from the 15th day after gestation till 15 days after birth and it was highest in the neonatal rats. These expression pattern showed similar to those that shown in the immunoistochemical results.The present results suggest that MT possibly contributes to parenchyma cell proliferation and differentiation through the storage or the supply of various metal ions in the developing rat liver.

歸因相談과 能力別 豫習課題의 活用을 通한 歸因性向의 變化가 數學學習 能力에 미치는 效果

오후진,구완규 공주대학교 사범대학 과학교육연구소 1999 과학교육연구 Vol.30 No.1

This study is trying to specifically search for the best approach ot learning motivation which is one of the most significant factors in improving learning ability, based on attribution theory (locus of control theory). As a result, the conclusions are as follows: 1. The procedure of Individual counseling for learning attribution based on individual standard grades and data of the variable order of merit apparently shows learners that if learners are to try their best in learning, they will surely go far in terms of learning in the near future. 2. The procedure of Individual counseling for learning attribution based on achievement distribution in individual behavior-oriented fields suggests to learners that how to learn is as important as how much effort they make. Surely enough, learners are required to make more effective and efficient efforts, considering their own learning abilities. 3. Above all, it was confirmed that the learners' struggling attitude might well have a significant correlation with achievement sucess.

최근 한반도 여름철 강수량 특성 분석

오완탁 조선대학교 환경공해연구소 2004 環境公害硏究 Vol.18 No.-

The recent Korean summertime weather characteristics is investigated with focus on heavy rainfall. It is found that the variation of the summertime pressure system in Korean Peninsula recently shows a different feature from its climatological variation, affecting the summertime rainfall pattern. Climatologically, the summertime rainfall concentrated in Changma period. Recently, the activity of Changma shows an irregular pattern and there are more rainfall in post-Changma period than in Changma period. Moreover, in terms of the rainfall mechanism, the heavy rainfall induced by the unstable atmospheric condition and topographic effect occurs more frequently than the rainfall induced by the lasting and regular system associated with the Changma.

제주도의 주요 습지에 도래하는 도요·물떼새류의 현황

오홍식,임인추,김병수,김완병,박행신 경희대학교 한국조류연구소 2002 연구보고 Vol.8 No.1

본 연구는 제주도 주요 습지에 도래하는 도요ㆍ물떼새류의 지소적인 연구의 일환으로, 1998년 3월부터 2000년 2월까지 2년간 매월 2회씩, 1) 동부지역(하도리 양어장, 종달리 해안 및 성산포 양어장)과, 2)서부지역(용수리 저수지, 금등리 해안 및 대정읍 일과리 해안)으로 나누어 조사하였다. 조사기간 중 2개 지역에서 관찰된 도요ㆍ물떼새류는 총 45종 5,117개체였으며, 지역별로는 동부지역에서 40종 4,910개체, 서부지역에서 38종 927개체로 나타났다. 계절별로는 봄철 2,821개체, 가을철 1,229개체로 봄철에 도래하는 개체수가 가을철보다 많았다. 우점종은 제1차 조사기간인 봄철에는 민물도요 Calidris alpina 2,024개체(88.5%), 흰물떼새 Charadrius alexandrinus 84개체(3.7%), 흰목물떼새 Charadrius placidus 11개체(2.4%)의 순으로 나타났으며, 가을철에도 민물도요 384개체(82.9%), 흰물떼새 20개체(4.3%), 흰목물떼새 11개체(2.4%)의 순으로 나타났다. 제2차 조사기간에는 봄철에 좀도요 Calidria ruficollis 116개체(21.8%), 민물도요 67개체(12.6%), 청다리도요 Tringa nebularia 58개체(10.9%)의 순으로, 가을철에는 민물도요 191개체(24.9%), 노랑발도요 T. brevipes 156개체(20.4%), 삑삑도요 T. ochropus 104개체(13.6%)의 순으로 나타났다. 동부지역은 서부지역에 비하여 우점도는 높았으나, 종다양도와 균등도는 낮게 나타났다. 동부지역과 서부지역간의 유사도는 0.85였다. 본 연구를 통해 물꿩 Hydrophasianus chirurgus, 검은머리물떼새 Haematopus ostralegus, 바늘꼬리도요 Gallinago stenura, 흰꼬리좀도요 Calidrius temminckii 등 4종의 제주도 미기록종과, 검은머리물떼새, 흰목물떼새, 넓적부리도요 Eurynorhynchus pygmeus, 알락꼬리마도요 Numenius madagascariensis 등 3종의 환경부 지정 보호조류가 관찰되었다. This study was part of a continuous research project on the status of migrating shorebirds on major wetlands in Cheju Island. This study was conducted twice every month for two years, from March 1998 to February 2000. The investigated location are mainly two areas. The first census areas were Hado-ri fishfarm and the coast of Jongdal-ri in Kujaw-up and Sungsan-po fishfarm in Sungsan-up, all located in the eastern part of Cheju Island. The second census areas were Yongsu-ri reservoir and the coast of Kumdung-ri in Hangyoung-myon and the coast of Ilgwa-ri in Dejong-up, all located in the western part of Cheju Island. In total, 45 species consisting 5,117 individuals were observed during the census period. There were 40 species and 4,910 individuals in the eastern area, 38 species and 927 individuals in the western area. The number of individuals of shorebirds in the spring migratory season was greater than in the autumn. The dominant species researched during the first census period were 2,024 individuals of Calidrius alpina (88.5%), 84 individuals of Charadrius alexandrinus (3.7%), 59 individuals of Charadrius placidus (2.6%) in spring and 384 individuals of Calidrius alpina (82.9%), 20 individuals of Charadrius alexandrinus (4.3%), 11 individuals of Charadrius placidus (2.4%) in autumn. The dominant species researched during the second census period were 116 individuals of Calidrius ruficolis (21.8%), 67 individuals of Calidrius alpina (12.6%), 58 individuals of Tringa nebularia (10.9%) in spring and 191 individuals of Calidrius alpina (24.9%), 156 individuals of Tringa brebipes (20.4%), 104 individuals of Tringa ochropus (13.6%) in autumn. Dominance index were recorded better in the eastern area than the western area, but species diversity and evenness index were recorded lower in the eastern area than the western area. Similarity index were 0.85 between the eastern area and the western area. Hydrophasianus chirurgus, Haematopus ostralegus, Gallinago stenura and Calidrius temminuckii, which are observed during the census period are previously unrecorded species on Cheju Island. Rare and endangered species were observed such as Haematopus ostralegus, Charadrius placidus, Numenius madagascariensis, and Eurynorhynchus pygmeus.

경피신경 전기자극이 β-Endorphin 및 Catecholamines치에 미치는 영향

吳洙根,김완식 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.1

The development of a method for the detection or highly specific opiate receptors in neural memberances and the discovery of such receptors in animal brains opened the current era of explosively rapid progress in understanding the fundamental mechanisms of action of the opiate narcotics. Furthermore a second advance was the discovery of two endogenuous opioid peptides, Met-and Leu-enkephalin which was recognized as a component of a larger peptide malecule, β-lipotropin. Immediately, scientists became interested in the possible physiologic function of these components in animal and humans, so active research in the rea was begun, and is continuing today. And also the relation-ships of endorphin to other neurotransmitter systems. Recently major efforts have been undertaken to develop assays for β-endorphin and catecholamines in plasma to study possible effcts of pain, stress, diurnal variation and acticity. It was a well known fact that βendorphin and catecholamines were secreted concomitantly by the pituitary gland. However, reserachers have noted an increase or decrease of β-endorphin and catecholamines in plasma after TENS, there may or may not be the correlation between acute and chronic pain. This study has undertaken the correlation between β-endorphin and catecholamines level changes by the TENS in the chronic pain and normal healthy volunter subjects with twelve cases each. We measured and observed β-endorphin levels with RIA KIT and catecholamines levels with High Performance Liquied Chomatography with Electrochemical Detection and also observed the changes of the blood pressure and pulsa rate before, after 30 minutes, and after 60 minutes with TENS. The results were as follows: 1. There were no significant differences on age, heigh and weight in compared with groups. (Table 1) 2. The pain intensity was markedly improved in the experimental gorups. (Table 1) 3. The changes of blood pressure were observed with slight decrease (P>0.05) and pulse rate were significantly decreased (P<0.05) in both groups before and after TENS. (Table 2) 4. Some changes with β-endorphin were observed with a slight increase or decrease in both groups before and after TENS< but there was no statistically significant changes in both gourps. (P>0.05) (Table 3) 5. Some changes in the catecholamines level were observed with a slight increase or decrease in both goups before and after TENS< but there was no constant tendency. (p>0.05) (Table 4). These results show that the relief of pain intensith with TENS were not correlated with β-endorphin and catecholamines level which has no constant tendency.

생리식염수 전기분해에 의한 세균 오염된 콘택트렌즈의 살균

김완석, 박준오, 최영기, 이영민, 김형규, 차은종, 김석용 충북대학교 동물의학연구소 2007 Journal of Biomedical and Translational Research Vol.8 No.1

본문참고

수돗물염수액 전기분해에 의한 살균소독

박준오, 김완석, 최영기, 이영민, 김형규, 차은종, 김석용 충북대학교 동물의학연구소 2008 Journal of Biomedical and Translational Research Vol.9 No.2

본문참고

원자의 선택적 2광자 공명성 3광자 이온화 과정에 관한 밀도행렬의 수치해

오완영,남백일,강석찬,김영순 명지대학교자연과학연구소 1991 자연과학논문집 Vol.9 No.-

2광자 공명성 3광자 이온화 과정에서 밀도행렬의 시간에 대한 미적분방정식을 수치해 석적으로 계산하여 기저상태와 2광자 공명 여기상태의 전자밀도 행렬요소 σ??와 σ??,이온화율 P, 그리고 동위원소 선택율 S를 Li과 Sr원자들에 대하여 계산하였고, Rate Approximation에 기초한 준정상해의 결과와 비교하였다. 비교적 큰 동위원소이동 값을 갖는 ??Li와 ??Li의 경우 레이저의 세기가 매우 작은 영역과 큰 여역에서는 P와 s의 수치해의 계산결과와 준정상해법 계산결과는 비교적 잘 맞는 경향을 볼 수 있었으며, ??Sr와 ??Sr의 경우 즉, 동위원소 이동값이 700MH?에 불과한 경우 Li원자보다 민감하여 준정상해법의 결과는 제한적으로 적용됨을 볼 수 있었다. We present results of numerical calculations obtained through solving integro-differential equations for the electron density matrices ?? and?? and the ionization rate P in two-photon resonant three-photon ionization of Li and Sr, as well as the selectivity S for the isotope separation. We also compare our results with the predictions from quasi-stationary solutions based on the rate approximation. Our numerical results for P and S show that the quasi-stationary solutions are valid at very high and very low laser intensities when the isotope shift is large as in the case with 4s of ??Li and ??Li, and that the validity is rather limited in the cases with small isotope shift as in 5p? ?S of ??Sr and ??Sr.

유전자 알고리즘 기반 퍼지 다항식 뉴럴네트워크의 설계

吳聖權,李寅太,金完洙 水原大學校 2005 論文集 Vol.23 No.-

In this paper we propose a new architecture of Fuzzy Polynomial Neural Networks(FPNN) by means of genetically optimized Fuzzy Polynomial Neuron(FPN) and discuss its comprehensive design methodology involving mechanisms of genetic optimization. especially Genetic Algorithms (GAs). The conventional FPNNs developed so far are based on mechanisms of self-organization and evolutionary optimization. The design of the network exploits the extended Group Method of Data Handling(GMDH) with some essential parameters of the network being provided by the designer and kept fixed throughout the overall development process. This restriction may hamper a possibility of producing an optimal architecture of the model The proposed FPNN gives rise to a structurally optimized network and comes with a substantial level of flexibility in comparison to the one we encounter in conventional FPNNS. It is shown that the proposed genetic algorithms-based Fuzzy Polynomial Neural Networks is more useful and effective than the existing models for nonlinear process. We experimented with Medical Imaging System(MIS) dataset to evaluate the performance of the proposed model.

토양으로부터 항세균성 물질을 생산하는 Streptomyces sp. 의 분리

오현정,정완석,김세재,김창진,고영환 제주대학교 생명과학연구소 1999 제주생명과학연구 Vol.2 No.-

제주도의 토양에서 분리된 방선균 307주를 대상으로, paper disc법과 시험관 희석 배양법을 사용하여 항세균성 물질을 생산하는 균주를 선별하였다. 약 3.3%에 상당하는 분리주들이 Escherichia coli 8749, Staphylococcus aureus 6538 그리고 Pseudomonas solanacearum 10692에 대해 항균효과를 나타냈고, 그 중에서 가장 높은 항균활성을 나타내는 BL93 균주를 최종선별하여 동정하였다. BL93 균주의 배양학적, 형태학적, 생리학적, 생화학적 그리고 화학분류학적 특성을 기준으로 볼 때 Streptomyces violaceus와 유사하였기에, Streptomyces sp. BL93으로 명명하였다. 이 균주가 생산하는 항세균성 물질은 butanol보다도 물에 더 잘 녹는 극성화합물로 chloroform이나 ethylacetate에는 거의 용해되지 않았다. 100℃에서 30분간 가열 처리해도 활성의 소실이 거의 없었고, pH 3∼9범위에서 안정하였다. 분리균주 BL93은 aspartocin을 생산하는 Streptomyces violaceus와 유사함으로, BL93이 생산하는 항세균성 물질의 동정 및 aspartocin과의 비교 검토가 선행되어야 할 것이다. Three hundred and seven isolates of Actinomycetes were isolated and purified from soil samples in Cheju Is. to screen antibacterial compounds. About 3.3% of the isolates showed antibacterial activity, and one of them, BL93, showed relatively higher antibacterial activity against Escherichia coli 8749, Staphylococcus aureus 6538 and Pseudomonas solanacearum 10692 by both paper disc method and tube dilution incubation method. The isolate BL93 was similar to Streptomyces violaceus based upon its cultural, morphological, physiological, biochemical and chemotaxanomic characteristics. It was designated as Streptomyces sp. BL93. The antibacterial substance produced by the isolate BL93 had higher affinity for water than butanol and was insoluble in chloroform or ethylacetate. Most of its antibacterial activity was retained even after heating at 100℃ for 30min and was stable at pH ranges 3 to 9. As the isolate BL93 was tentatively identified to be Streptomyces violaceus, the antibacterial substance preferentially need to be identified and compared with aspartocin, an antimicrobial compound produced by Streptomyces violaceus.