다형홍반증에서 각질형성세포의 ICAM-1, TNF-α 및 IL-1α 발현에 관한 연구

황규왕,정현,이종석,박준홍,부태성,이성열 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.1

The pathogenetic mechanisms involved in the developement of erythema multiforme(EM) are still largely unknown. The histologic and immunopathologic changes in erythema appear to be due in part to cellular immune mechanisms with the cytotoxic T cell. Especially cytotoxic T cell/ICAM-1 positive-keratinocyte adherence plays an important role in the pathogenesis of EM. In this study, we investigated the expression of TNF-a, IL-1 a by immunohistochemical stain and ELISA. In skin tissue of EM, strong basal cell expression of ICAM-1, TNF-a and IL-1 a was found, and we detected more TNF-a from supernatants of cultured human keratinocytes with serum of EM patient than normal control. Our results may suggest possible relationship between the pathogenesis of EM and cytokine from keratinocyte.

鋼內 介在物의 Br-化合物 形成에 關하여

黃龍吉,李相允,金營三,成章鉉 東亞大學校 大學院 1980 大學院論文集 Vol.4 No.2

The results obtained from the experiment to make a comparison between thermal characteristics and chemical reactions of 10% Br-Methanol treated oxides, and inclusions and oxides in 18Cr-8Ni stainless steel can be concluded as follows; 1. It has been shown that when MnO2, Cr2O3, Fe2O3 and SiO2 are heated, they all give exothermic characteristics in the temperature range about 200 to 300℃, but they tend to be heated up endothermically in the range 300 to 900℃, except for the fact that when the reaction 3MnO2=Mn3O4+O2 occurs at 600℃, peak showing endothermic reaction is observed. 2. Specimens made by mixing some of oxides such as MnO2, SiO2, Fe2O3 and Cr2O3 and uheir complex compunds and inclusins have all produced similar reactions which are characterized by showing an exothermic for temperatures of up to approximately 300℃ and an endothermic for the temperature range 300 to 900℃. However, it is noted that some oxides containing Mn give a sharp peak which represents endothermic reaction. 3. Below 100℃ the thermal analysis curves obtained from specimens made by reacting each complex compound with 10% Br-Methanol have been shown to be similar to these for specimens not rected with 10% Br-Methanol, showing endothermic heating for temperatures from about 150 to 200℃ endothermic heating for higher temperatures than 200℃. 4. The X-ray diffraction analysis has shown that oxides such as Mn3O4 and Cr2O3 are observed from the complex compound with a ratio MnO2: Cr2O3=1:1, whereas in the case of the compound with a ratio MnO2:Cr2O3=2:1 peak for Mn3O4 only is observable without any trace for Cr2O3 on the diffraction chart. Furthermore in the compound of Fe2O3 and Cr2O3 the peak for Cr2O3 has been determined, but no peak for Fe2O3 can be found. 5. The x-ray diffraction analysis has also presented that in inclusions in steel and complex oxides extracted by using 10% Br-Methanol the inclusions give peaks for Cr2O3 only, and peaks for Mn3O4 and Cr2O3 are obtainable. Based on this observation only when inclusions in steel are extacted by using 10% Br-Methanol amorphous structure is formed by Br, but crystalline oxides are thought to be unchanged to the amorphous.

플라즈마 표지소자의 제작

이상윤,라병욱,박동수,황인헌,이덕동,신영남,박성배,이동욱,박용석,박형근,손상호,권태근,채경락,정경득 慶北大學校 自然科學大學 1986 自然科學論文集 Vol.4 No.-

An Ac-type Plasma Display Panel (PDP) operating with Ne-Ar(0.1%) Penning mixture gas is fabicated. The characterics of the panel with electrodes covered with thin and thick dielectric layers are studied. The brightness of the Neon-orange light emitted by the panel measured as function of applied voltage and frequency. As an application, a graphic display system equipped with PDP showing still and moving pictures is made.

인체 흑색종 세포군에서의 Vascular Endothelial Growth Factor(VEGF) 분비

김현조,황규왕,조문균,박영립,이종성,이성열 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Backgroung : Vascular endothelial growth factor(VEGF) is an angiogenic cytokine expressed by many human and animal tumors. Ultraviolet radiation(UVR) has been causally linked to melanoma initiation and progression. UVR is a known melanocyte mutagen and induces specific mutations in cellular oncogenes such as p53 and ras. Activated H-ras activates tumor angiogenesis in other cell types, and angiogenic switching is accompanied by up-regulation of VEGF. Objective : The purpose of this study was to determine whether VEGF release from human melanoma cell lines was altered by activating ras mutations or by inactivation of p53 induction and whether VEGF release of these transfected melanoma cell lines was changed after UVR radiation. Results : 1. Release of VEGF from menolayer culture of melanoma cells was significantly higher in cells with activating ras mutations and lower in cells with expression of dominant negative p53. 2. In spheroid culture, dominant negative p53 decreases VEGF release in WM35 cells, but activating ras did not affect VEGF release significantly. 3. The control cell line(WM35Neo) had higher VEGF release in the spheroid culture than in the monolayer culture, and dominant negative p53 or activating ras did not affect VEGF release significantly between these two culture systems. 4. Higher cell density increases VEGF release in all tested melanoma cell lines was shown in both spheroid and monolayer culture. This cell density effect was strongest in cells with activated ras in monolayer culture. 5. UVR inhibits VEGF release in all tested melanoma cell lines, and this effect was stronger in monolayer culture than in spheroid culture. The effect of UVR on VEGF release was significantly lower in the DNp53 transfectants. 6. UVR decreased the cell viability in a dose dependent manner regardless of the cell density or cell culture system. Conclusion : VEGF release in human melanoma was inhibited by ultraviolet radiation at cytotoxic doses, and this effect is stronger in monolayer culture than in spheroid culture. VEGF release from melanoma cell lines was also influenced by p53, ras and cell growth conditions in a complex manner.

아토피 피부염 환자에서 혈청내 Cu와 Zn치 및 Cu/Zn비에 관한 연구

이성열,정현,이종석,황규왕 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.1

아연과 구리는 피부를 포함하는 인체의 거의 모든 기관에서 일어나는 대사과정에 작용하는 metallonenzyme의 보조인자로 상당히 중요한 역할을 수행한다. 저자들은 정상대조군 10명, 전신성 아토피성 피부염 환자 16명을 대상으로 atomic absorption spectrometry를 사용하여 혈청 아연과 구리치를 측정하였고 그결과로 혈청 구리/아연 비를 구하였다. 그 결과 혈청 구리치는 대조군에 비하여 아토피성피부염 환자에서 통계적으로 의의있게 높게 나타났다. 이는 아마도 아토피성 피부염에 의한 염증반응으로 이차적으로 ceruloplasmin이 증가되어 나타난 것으로 생각되나 이 것이 명확히 규명된 것은 아니다. 혈청 아연치는 약간 감소하였으나 통계적인 유의성은 없었고 혈청 구리/아연 비의 값은 약간 증가되었으나 역시 통계적 유의성을 찾을 수는 없었다.

菟絲子 의效能에 대한 硏究

진상식,황의현,김형창,김천수,오광수,한종현 한국전통의학연구소 2002 한국전통의학지 Vol.12 No.1

The purpose of this study is to determine the effect of Semen Cuscutae on phenylephrine(PE) and KCI induced contraction of isolated rat thoracic aorta. Contractile force was measured with force displacement transducer under 1.5 g loading tension. Contraction evoked by PE (ED_(50)) was relaxed significantly by Semen Cuscutae. The mean percent relaxing of PE induced contraction was 60.6% (p<0.01) after 300 ㎕/㎖ Semen Cuscutae, but the mean percent contraction of KCI induced contraction was 10.4% (p<0.05) after 300 ㎕/㎖ Semen Cuscutae. Vasorelaxation by Semen Cuscutae was significantly reduced after with and without endothelial cells. Pretreatment of aortic rings with L-NAME (100 μM), a nitric oxide synthase inhibitor, indomethacin (3 μM), an inhibitor of cyclooxygenase, methylene blue (10 μM), guanylyl cyclase inhibitor, propranolol (1 μM), a β-adrenoceptor antagonist, atropine (1 μM), a muscarinic receptor antagonist and diltiazem (10 μM), a Ca^(2+)-channel blocker was recovered the effect aorta. These results indicate that Semen Cuscutae can relax EP induced contraction of isolated rat thoracic aorta and that this increasing contraction related to epithelium and various effect.

무색소성 색소 실조증(Ito 저색소증) 1예

방성원,이정열,황규왕,정현,김영근 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.1

Incontinentia pigmenti achromians(IPA) or Hypomelanosis of Ito is a very rare neurocutaneous syndrome which is often associated with cutaneous, cerebral, musculoskeletal and ocular abnormalities. It is assumed that it is autosomal dominant of inheritance in character. There is also a relationship between IPA and incontinentia pigmenti(IP). However, the etiology of IPA is not known. We describe a 4-year-old girl with myopia, esotropia and retinal pigmentary degeneration in addition to the hypopigmented skin lesion of IPA, and reviewed clinically the cases of IPA in this article.

효율적인 분할 시그너춰 화일의 디렉토리 관리

문현수,황환규,최황규,염상민,김상욱 강원대학교 정보통신연구소 1998 정보통신논문지 Vol.2 No.-

A partitioned signature file is an enhancement of the signature file that divides all the signatures into blocks in such a way that each block contains the signatures with the same key. Its directory stores all the keys as meta information for avoiding unnecessary block accesses by examining them first before the actual searching of the blocks. Efficient directory management is very important in large database environments since its size gets larger proportionally to that of the database. In this paper, we first point out the problems in the directory management methods of the previous partitioned signature files, and then pressent a new one solving them. Our method offers good features in the following three aspects: (1) suitability for large database environments, (2)adaptability to dynamic situations, and (3) storage overhead for the directory.

Lutembacher 증후군 환자 증례 보고

김성범,서정주,곽철훈,김상민,이보라,민선경,황은구,김용인,조욱현,최석구 인제대학교 2008 仁濟醫學 Vol.29 No.-

Lutembacher's syndrome is rare combination of mitral stenosis (MS) and atrial septal defect (ASD). The hemodynamic interplay between the MS and ASD leads to wide variation of clinical presentation. Here we describe a 43-year-old female with Lutembacher's syndrome and tricuspid regurgitation with pulmonary hypertension, who underwent direct closure of ASD and tricuspid valvuloplasty successfully. We also reviewed other literatures in an effort to increase awareness of this condition.

충청남도 지역에서 진단된 광선 각화증에 대한 임상 및 병리조직학적 고찰

김성근 ( Sung Keun Kim ),신정웅 ( Jung Woong Shin ),이성열 ( Sung Yul Lee ),이상훈 ( Sang Hoon Lee ),조문균 ( Moon Kyun Cho ),황규왕 ( Kyu Uang Whang ),박영립 ( Young Lip Park ),남두현 ( Doo Hyun Nam ),조현득 ( Hyun Deuk Cho ) 대한피부과학회 2010 大韓皮膚科學會誌 Vol.48 No.1

Background: Actinic keratoses (AK) have been recognized as precancerous lesions, they have been recently considered to be an early in situ squamous cell carcinoma (SCC). However, there have been few studies to determine the clinical and histopathological characteristics of AK, and especially in the Province of Chungcheongnam-do. Objective: The purpose of this study was to evaluate the clinicopathological characteristics of 80 cases of AK in the province of Chungcheongnam-do. Methods: The authors evaluated the clinical and histopathological features of 80 patients with AK at Soonchunhyang University Hospital in Cheonan from January 1998 to December 2008. Results: The ratio of male to female patients was 1:4.7. The age distribution ranged from 42 to 93 years old. Single lesions are more common than multiple lesions. The duration of lesions was variable with a mean duration of 25.1 months. The most commonly involved sites were the face and especially the cheek. The color was erythematous in 63.3% of the cases, brownish in 24.5%, dark-brownish in 9.5% and gray in 2.7%. Crust and scale were noted in 37.4% and 22.4% of the cases, respectively. SCC was associated with AK in 6.3% of the cases, basal cell carcinoma was associated with AK in 1.3%, and keratoacanthoma in 1.3%. The most common histopathological types of AK were atrophic types in 20.6%, and this was followed by the hypertrophic types in 18.6% of the cases and the hypertrophic and atrophic types in 14.7% of the cases. Conclusion: Our results are similar to those of the previous reports. In addition, we observed a consistently higher incidence in females than males in the Korean reports and a more frequent incidence of single lesions than multiple lesions in the Asian reports. We found that AK is not easily distinguishable from SCC both clinically and cytologically. Thus, we suggest that biopsies and active treatments should be performed on AK lesions. (Korean J Dermatol 2010;48(1):1∼7)