더덕의 효율적인 재분화 및 뿌리발달

심주선,조숙녀,손화,김무성,노영덕,김세영,양덕춘 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

더덕은 식용, 관상용, 생약재료로 쓰이는 방향성 식물이다. 본 연구는, 종자의 기내배양을 통하여 식물체 재분화와 뿌리발달을 실험했다. 종자의 발아는 GA_(3) 100 mg·L(-1)처리와 저온처리 시 매우 양호하였다. 더덕의 기내에서 shoot의 형성은 2 mg·L^(-1) BA, 2 mg·L(-1) NAA가 첨가된 MS 기본배지에서 가장 양호하였으며, 줄기의 절편체와 비교하여 잎 절편체에서 재분화가 잘되었다. 또한 뿌리의 발육은 계속적인 계대배양에 의해서 양호하였는데 shoot의 길이를 1 cm 정도 남긴 뿌리를 연속적으로 4회 계대배양에서 뿌리가 주근으로 발육하였으며 길이는 약 3 cm 및 직경은 0.5 cm의 정상적인 뿌리를 얻을 수 있었다. Codonopsis lanceolata is widely used as edibles, ornamental plant and crude drug material. This study was carried out to confirm the ratio of germination and efficient differentiation of shoot and root in vitro. The germination of Codonopsis lanceolata seed was excellent in the medium with GA_(3) 100 mg·L(-1) and chilling treatment. The shoot formation of Codonopsis lanceolata was good at the MS medium with 2 mg·L(-1) BA and 2 mg·L(-1) NAA. According to inoculum part, ratio of re-differentiation was higher from the leaf explant compared with the stem explant. For root development, successive subculture with 1 cm shoot of upper part from root was efficient. Roots of Codonopsis lanceolata cultured in vitro system was grown like normal root with length of 3 cm and thickness of 0.5 cm at the 4 successive subcultures.

프로그램 선언부를 위한 심벌테이블에서 C 프로그램 역번역기의 설계 및 구현

권혁주 ( Hyeok-ju Kwon ),김영근 ( Young-koun Kim ),이양선 ( Yang-sun Lee ),오세만 ( Se-man Oh ) 한국정보처리학회 2005 한국정보처리학회 학술대회논문집 Vol.12 No.1

ANSI C 언어는 UNIX 시스템에서 뿐만 아니라 DOS 환경에서 수행되는 C 컴파일러와 각종 지원 도구가 개발되어 보급됨으로써 오늘날 널리 사용되는 범용 프로그래밍 언어 중 하나이다. EVM(Embedded Virtual Machine)은 ANSI C 언어와 SUN사의 Java 언어 등을 모두 수용할 수 있는 임베디드 시스템을 위한 가상 기계이며, SIL(Standard Intermediate Language)은 EVM에서 실행되는 중간언어로 다양한 프로그래밍 언어를 수용하기 위해서 객체지향 언어와 순차적 언어를 모두 수용하기 위한 연산 코드 집합을 갖고 있다. EVM을 위한 ANSI C 컴파일러는 ANSI C 언어를 받아 들여 EVM의 중간 언어인 SIL 코드를 출력한다. ANSI C 컴파일러에서 어휘 분석과 구문 분석 과정에서 인식되는 명칭에 대해서 그 속성들을 수집하고 이용한다. 이 속성들은 명칭이 명시적으로 혹은 묵시적으로 정의되는 곳에서 심벌 테이블에 수집된다. 본 논문에서는 수집된 정보가 올바르게 되었는지 확인하기 위하여 심벌 테이블에 있는 정보를 다시 ANSI C 언어로 복원시키는 역변역기(detranslator)를 구현하였다.

극배치에 의한 직류 서보 모터의 위치제어시스템 설계

김태균,양희주,김규로,선양래 慶熙大學校 大學院 1995 高凰論集 Vol.16 No.-

In this paper, the pole placement design theory is applied to position control system of DC servo motor. The DC servo motor is modelled by second order linear time invariant system. We assume that All state variables are measurable and are available for feedback. If the system considered is completely state controllable, then poles of the close-loop system may be placed at any desired locations by means of state feedback through an appropriate state feedback gain matrix K. This design method called the pole placement. Proposed pole placement design has superior performance in unit step response. The pole placement design theory is very useful to drive the position control system of DC servo motor.

간호사의 이직의도 구조모형

김창희,양승순,김연자,손연정,유미애,송주은 대한간호학회 간호행정학회 2009 간호행정학회지 Vol.15 No.4

Purpose: The purpose of this study was to construct and test a structural equation model of nurses' turnover intention. Method: The participants were 716 nurses at 4 university hospitals. Data were collected by a self-report questionnaire in 2008 and analyzed using SPSS 12.0 and AMOS 4.0 Win program. Results: The fit indices of modified model were x²=285.928(p<.001),x²/df=1.958GFI=0.964, RMSEA=0.037, AGFI=0.944, NFI=0.947, NNFI=0.961, CFI=0.973, PNFI=0.658 and showed high compatibility with the empirical data. In the final model, job stress and satisfaction were found to have significant direct effects on nurses' turnover intention, while organizational culture and emotional labor were very important factors to have indirect effects on turnover intention via job stress and satisfaction. Nurses' turnover intention was accounted for 41.2% of covariance by these factors. Conclusions: Comprehensive nursing interventions for decreasing nurses' turnover intention should be focused on reducing job stress and increasing job satisfaction. Also, Nursing strategy for enhancing positive organizational culture and decreasing emotional labor would be helpful for decreasing nurses' turnover intention, too. Further studies are needed to prospectively conduct to verify these causal relationships with larger sample.

인삼 Peroxidase(PgPrx3) 유전자의 분리 및 연초의 형질전환

손화,심주선,김세영,노영덕,김무성,양덕춘 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

Peroxidase는 항산화에 관련된 효소이며 식물의 환경스트레스와 성장에 관련되는 중요한 유전자이다. 본 실험에서 사용된 Peroxidase Ⅲ의 cDNA는 Panax ginseng C.A. Meyer 잎에서 추출하였으며 PgPrx3라 명명하였다. 이 gene의 ORF영역은 1,065 bP이고 355개의 아미노산을 가지고 있다. BioEdit 프로그램을 이용하여 PgPrx3와 다른 식물의 peroxidase gene과 비교한 결과 상동성을 가지고 있었으며, Spinacia oleracea(70%), Vigna angulais(71%), Nicotiana tabacum(69%) and Linum usitatissimum(65%). 그중 Vigna angularis의 상동성이 가장 높았다. 담배에 본 유전자를 형질전환시켜 유전자의 특성을 검정하기 위하여 35S/35S/AMV/peroxidas/Tnos를 벡터 pRD400에 재조합 후 아그로박테리아를 이용하여 담배에 도입시켰다. 그리고 PCR를 이용하여 유전자 PgPrx3가 담배에 도입된 것을 확인하였으며, RT-PCR로 정상적으로 PgPrx3 유전자가 전사되어 RNA를 생성하고 있음을 확인하였다. A peroxidase (E.C. 1.11.1.7) is very important enzyme as antioxidants. The function of this gene is connected with growth and environmental stress of plant. A class Ⅲ peroxidase cDNA was isolated from the leaf of Panax ginseng C.A. Meyer and named as PgPrx3 which is consisted of an ORF (open reading frame) of 1,065 bp and an amino acid of 355 residues. BioEdit software was used to compare the PgPrx3 amino acid sequence with other plants that showed homologies with Spinacia oleracea (70%), Vigna angularis (71%), Nicotiana tabacum (69%) and Linum usitatissimum (65%). The peroxidase of Vigna angulatis was the most homologous with ginseng. The chimeric PgPrx3 gene, 35S/35S/AMV/peroxidas/ Tnos, was constructed in the binary vector pRD400. Agrobacterium tumefaciens strain LBA4404 harboring disarmed Ti-plasmid was used to transform Nicotiana tabacum L. using the leaf disc. Incorporation of the chimeric gene PgPrx3 into plants were confirmed by PCR analysis of genomic DNA and RT-PCR analysis of mRNA from transgenic tobacco. The PgPrx3 gene from ginseng was stably expressed in transgenic tobacco plants.

뇌기저동맥 첨단부 폐쇄증후군 후 지연성 올리브 비대에 동반된 눈떨림

김영진,정주리,강성원,한선정,이성익,손일홍,양현덕 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

눈입천장떨림(Oculopalatal tremor)은 치아핵, 적핵 및 올리브핵을 연결하는 신경로(dentatorubro-olivary pathway, Guillain-Mollaret triangle)의 손상 이후에 발생하는 비대성올리브핵 변성(hypertrophic inferior olivary degeneration)에 의한 지연성(delayed) 합병증이다. 다리 뇌출혈(pontine hemorrhage) 또는 뇌줄기경색(brainstem infarction)후 아래 올리브핵비대(inferior olivary hypertrophy)가 발생한 환자에서 입천장떨림(palatal tremor) 없이 눈떨림이 보고된 경우가 있으나, 뇌바닥동맥끝증후군(top of the basilar syndrome) 후 아래올리브핵비대가 발생한 환자에서 눈떨림이 보인 경우는 드물다. 뇌바닥동맥끝증후군 후에 아래올리브핵비대가 발생한 환자에서 지연성 수평 눈떨림 보고하고자 한다. 이것은 양측 소뇌다리의 손상 때문에 눈떨림의 수직 성분이 동반되지 않은 수평눈떨림을 보인 것으로 유추할 수 있다. Oculopalatal tremor(OPT) is a delayed complication of hypertrophic inferior olivary degeneration after the lesion in the dentato-rubro-olivary pathway, the Guillain-Mollaret triangle. Some cases of ocular tremor without palatal tremor associated with inferior olivary hypertrophy after pontine hemorrhage or brainstem infarction have been reported, but ocular tremor with inferior olivary hypertrophy after top of the basilar syndrome has rarely been described. We describe a patient showing delayed-onset horizontal ocular tremor with inferior olivary hypertrophy after top of the basilar syndrome. It can be postulated that the vertical component of ocular tremor could have not developed because of the lesions in bilateral cerebral peduncles.

어머니가 지각한 영아기질과 어머니 역할수행에 대한 자신감 및 만족도의 관계

이영은,강양희,박혜선,황은주,문미영 대한간호학회 아동간호학회 2003 Child Health Nursing Research Vol.9 No.2

어머니가 지각한 영아기질과 어머니 역할수행에 대한 자신감 및 만족도의 관계

이영은,강양희,박혜선,황은주,문미영 고신대학교 전인간호과학연구소 2003 전인간호과학연구 학술모음집 Vol.2 No.-

Method : The subjects of this study were 300 mothers of infant at the age of 1-12 months who visited well baby clinic in 4 hospitals in Busart city and Kyoung-Nam province. Final analysis was performed in 293 cases. Seven cases was excluded in this study because of its inappropriate data collection. The data was collected from 1st July to 15th August 2002. The questionnaries which were fill-up by mother were collected. Infant temperament was measured by using the tool of "what my baby is like''(WBL) which was developed by Priham et. al.(1994) and translated by Bang(1999). The scale of postpartum self evaluation which was developed by Lederman et al( 1981) and translated by Lee( 1992) was used for the confidence and satisfaction of maternal role. All statistical analyses were performed using SPSS-PC for window, version 10.0: frequency, percentage, minimum, maximum, mean, SD, t-test, ANOVA, Post-hoc test(Scheffe's test), Pearson Correlation Coefficients. Purpose : This study was intended to search the relationship between perception of the infant temperament in mother of infant at the age of 1-12 months and maternal confidence and satisfaction in performing maternal role, and to submit a basic data to establish a nursing intervention program which is helpful for determination of infant development and performing maternal role promotion by identify variables associated with infant temperament perception of the infant temperament was 6.17±1.04, and mother recognized her infant as positive. The mean score of confidence of maternal role was 2.89±.41 and this revealed in an average level. The mean score of satisfaction of maternal role was 3.29± .51 and this revealed in a higher level. There was a weak significant positive correlation between the score of maternal perception of infant temperament and confidence of maternal role(r=0.176, P= .003), but there was no significant correlation between satisfaction of maternal role(P> .05). It revealed the more maternal perception of the infant temperament as positive, the higher confidence of maternal role. There was a moderate significant positive correlation between confidence of maternal role and satisfaction of maternal role(r=0.410, P= .000). It revealed the more confidence of maternal role, the higher satisfaction of maternal role. The variables related with the score of maternal perception of infant temperament were the type of delivery (t=-2.600, P= .010), experience of learning baby care(t=2.382, P= .018), maternal perception on baby's health status(F=3.467, P= .033), maternal perception on her health status(F=3.467, P= .027), baby's age(F= .080, P= .028). Conclusion : Our result showed the confidence of maternal role was increased as the maternal perception of infant temperament was positive, and conformed that the confidence of maternal role was also related with satisfaction of maternal role. Prenatal education, type of delivery, baby's age were also related with the maternal perception of infant temperament. So, nursing intervention program of developmental stage maybe necessary in order to help maternal perception of infant temperament as positive, and it will be increased the confidence of maternal role and satisfaction of performing maternal role which was considered as real indicate of achievement of maternal role.

교합 거상 금관이 어린이 교합 발육에 미치는 영향

신정근,백병주,김재곤,양연미,이선영 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.1

혼합 치열기에서 유치의 탈락 및 영구치의 맹출 과정이 순조롭게 이루어지는 것은 중요한 일이다. 맹출 중인 치아는 인접치유무, 유치의 흡수속도, 유치의 조기상실, 만기잔존, 국소병소나 입술, 혀, 저작근 등의 다양한 요인들에 의해 영향 받을 수 있으며, 이러한 요소들 사이의 균형이 깨지게 되면 인접치 간의 관계가 변하고 심각한 부정교합이 초래될 수 있다. 본 증례는 유치열 및 혼합치열을 가진 어린이들에서 상하악 구치부 교합면에 접착된 교합 거상 금속 스플린트(bite raising metal splint)가 치열에 미친 영향에 대해 보여주고 있다. 이들은 스플린트로 인한 유치의 만기잔존으로 영구치 맹출에 방해를 받거나, 전치부 개방교합, 제 1대구치의 저위 맹출 등 혼합 치열기 발달에 큰 영향을 받고 있었다. 치료로써 고속 핸드피스를 이용하여 스플린트를 제거하고 주기적 점검을 통해 교합변화 및 영구치 맹출 양상을 관찰하였다. Children are in mixed dentition during 6 years after 3 years old. this time is very important for sound permanent dentition. There are many factors of influence to tooth eruption stage ; adjacent teeth, tooth resorption, early loss or retention of deciduous tooth, local lesion, lip and tongue, masticatory muscles, ect. These factors should be in balance, if not, relation of adjacent teeth is changed, then severe malocclusion is occurred maybe. These cases revealed influences of resin bonded metal splint on occlusal surface of children's molar to mixed dentition. Splints interfere with falling off of deciduous tooth, tooth eruption, normal occlusion formation, and development of mixed dentition and occlusion. Therefore we removed the metal splint from teeth, follow-up checked occlusion and tooth eruption.

Lithium‐Sulfur Batteries: An Advanced Lithium‐Sulfur Battery (Adv. Funct. Mater. 8/2013)

Kim, Junghoon,Lee, Dong‐,Ju,Jung, Hun‐,Gi,Sun, Yang‐,Kook,Hassoun, Jusef,Scrosati, Bruno WILEY‐VCH Verlag 2013 Advanced functional materials Vol.23 No.8

<P>On page 1076, Yang‐Kook Sun, Jusef Hassoun, Bruno Scrosati, and co‐workers report and characterize a hard carbon spherules‐sulfur electrode that is prepared by impregnation of crystalline sulfur into the carbon spheres through melting and thermal treatment. This material demonstrates very high capacity and rate capability, and is therefore proposed as a new generation cathode to drive electric vehicles over a long range. </P>