다기관 전향적 임상연구를 위한 웹 데이터베이스 시스템

김혜림,최신영,유은미,박지숙 서울여자대학교 2010 정보기술논문지 Vol.8 No.-

Because many centers jointly select the subject, multi-center clinical study can promptly recruit various patient groups into the research, thus being advantageous in terms of generalization. However, prospective clinical study has difficulties in implementation, because it takes a lot of efforts to trace a large scale of subjects. The purpose of this study is to develop a web database system suitable for multi-center prospective clinical study. This study has presented three basic strategies-accurate data input, easy data tracing, and real-time data verification-to supplement the demerits of prospective clinical study. The developed system has been applied in the clinical study proceeded by the study group for Respiratory failure which is affiliated to The Korean Academy of Tuberculosis and Respiratory Diseases's Association for Research on Respiratory Failure and has been verified of its performance.

급냉 응고된 Al합금의 열처리에 따른 미세구조 및 기계적성질에 관한 연구

서동수,조성석,신용덕,김혜성,임승무 忠南大學校 産業技術硏究所 1988 산업기술연구논문집 Vol.3 No.2

Rapidly solidified A2024 alumium alloy plate and powder produced by the twin-roll method and the centrifugal atomization method, respectively. The microstructure and shape of the specimen were closely related to the process parameters. The process parameters of the twin-roll method must be properly combined in order to manufacture defect-free plate. Four kinds of powder shape were produced by the centrifugal atomization process, e.g. spherical, tear drop, ligamental and flake. The age hardening behavior of the rapidly solidified A2024 alloy was somewhat different from the conventional slowly solidified one.

황사 기간 동안에 어린이, 성인, 노인에서의 자각증상과 예방행동에 대한 조사연구

이보은,하은희,박혜숙,권지숙,신경림 韓國學校保健學會 2005 韓國學校保健學會誌 Vol.18 No.1

Purpose: Recently there are scientific evidences for the health effects of Asian dust-storms. Particularly in 2002, the daily average of PM_(10) exceeded 600 and 700 ㎍/㎥ in March and April respectively. We examined the effects of Asian dust-storms on perceived symptoms and preventive behaviors among children, adults, and elders. Method: The subjects of this study were 425 children, 444 adults and 60 elders. A questionnaire survey was carried out on the children and the adults by teachers and on the elders by interviewers to obtain information on demographic variables, perceived symptoms and preventive behaviors, previous respiratory disease, environmental tobacco smoke, and proximity between the house and the road during Asian dust-storms. We analyzed using descriptive statistics, X_(2) test and multiple logistic regression models. After adjustment for potential confounders, we estimated the odds ratios and 95% confidence intervals of the children and the elders for perceived symptoms and preventive behaviors. Result: The elders had a significantly higher prevalence of respiratory symptoms than the other groups. The children had a significantly higher prevalence than the adults in getting URI (1.51), coughing (1.68), rhinorrhea (1.46), fever (2.39) and medication for allergy or asthma (1.90). The children had better behaviors than the elders in closing windows (3.57), taking less outdoor recreational activity (2.59) and staying home (2.60).

Restless Genital Syndrome: A Rare Case Series of Four Patients and a Literature Review

Shin Hye-Rim,Han Sun Jung,정기영 대한수면학회 2020 sleep medicine research Vol.11 No.2

Restless genital syndrome (RGS) is a rare disorder with unpleasant sensation in the genital area and unwanted sexual arousal. Only a few cases of RGS have been reported, so we presented four cases of RGS. Four cases were all middle-age female, and was commonly complaint paresthesia on their genital area and unwanted sexual arousal. The typical symptoms of restless legs syndrome (RLS), affecting on their lower extremities were accompanied. RGS is difficult to diagnose because it presents ambiguous sensations that are difficult to describe, along with embarrassment in reporting sexual feelings. Clinicians should be aware of the genital discomfort and sexual arousal that can develop in RLS.

Pin1-mediated Modification Prolongs the Nuclear Retention of β-Catenin in Wnt3a-induced Osteoblast Differentiation

Shin, Hye-Rim,Islam, Rabia,Yoon, Won-Joon,Lee, Taegyung,Cho, Young-Dan,Bae, Han-sol,Kim, Bong-Su,Woo, Kyung-Mi,Baek, Jeong-Hwa,Ryoo, Hyun-Mo American Society for Biochemistry and Molecular Bi 2016 The Journal of biological chemistry Vol.291 No.11

<P>The canonical Wnt signaling pathway, in which -catenin nuclear localization is a crucial step, plays an important role in osteoblast differentiation. Pin1, a prolyl isomerase, is also known as a key enzyme in osteogenesis. However, the role of Pin1 in canonical Wnt signal-induced osteoblast differentiation is poorly understood. We found that Pin1 deficiency caused osteopenia and reduction of -catenin in bone lining cells. Similarly, Pin1 knockdown or treatment with Pin1 inhibitors strongly decreased the nuclear -catenin level, TOP flash activity, and expression of bone marker genes induced by canonical Wnt activation and vice versa in Pin1 overexpression. Pin1 interacts directly with and isomerizes -catenin in the nucleus. The isomerized -catenin could not bind to nuclear adenomatous polyposis coli, which drives -catenin out of the nucleus for proteasomal degradation, which consequently increases the retention of -catenin in the nucleus and might explain the decrease of -catenin ubiquitination. These results indicate that Pin1 could be a critical target to modulate -catenin-mediated osteogenesis.</P>

Synthesis and evaluation of new series of chalcones as inhibitor of interleukin-5

JUNG Sang-Hun,SHIN Hye-Rim,YANG Hyun-mo,BANG Seong-Cheol,LEE Ki-Cheul,SEO Hyun-Hee,KIM Byung-Hak,KIM Youngsoo 대한약학회 2006 大韓藥學會 總會 및 學術大會 Vol.2006 No.2

전처리 및 냉·해동 방법에 따른 냉이의 품질 특성 차이

신혜림 ( Hye Rim Shin ),박지현 ( Ji Hyun Park ),이상윤 ( Sang Yoon Lee ),박보람 ( Bo Ram Park ),한귀정 ( Gwi Jung Han ),최미정 ( Mi Jung Choi ),김하윤 ( Ha Yun Kim ) 한국산업식품공학회 2016 산업 식품공학 Vol.20 No.4

냉이를 장기 저장하며 품질을 유지할 수 있는 냉동 냉이를 개발하기 위해 데치기 및 냉해동 조건에 따른 품질특성 분석에 대해 조사하였다. 냉이의 데침 조건에 따른 이화학적 특성 분석을 통하여 최적 전처리 조건을 확립한 후, 데친 냉이를 함기포장하여 각각 -20℃, -40℃, 침지식(-60℃)에서 냉동하였고, 냉동 후 -20℃에서 7일 간 저장한 후 각각 상온해동(25℃), 유수해동, 고주파 해동하여 이화학적 특성을 분석하였다. 냉이의 원물 및 데친 냉이의 이화학적 특성을 분석한 결과 색의 변화를 최소화 하고, 미생물 안전성을 확보하기 위하여 30초간 데침 처리가 적절하다고 판단하였다. 적절한 조건으로 전처리한 냉이를 냉·해동 처리한 후 이화학적 특성을 분석한 결과 냉동 온도가 낮을수록 색 변화나 물성의 변화가 적은 것으로 나타났다. 해동 방법으로는 고주파 해동이 품질 변화가 적은 것으로 나타났다. The conditions of blanching, freezing, and thawing for Shepherd`s purse (Capsella bursa-pastoris [L.]) were studied to improve its quality attributes during frozen storage. The washed samples were blanched with water at 100℃ for 10, 20, 30, and 40 s. They were then soaked in cold water. The quality characteristics of the samples such as pH, texture, hardness, color value, and total viable cell were analyzed. The samples blanched for 30 s were frozen and thawed under several conditions and their physicochemical characteristics were analyzed. The pH value of raw sam-ples was higher than that of the blanched sample. The values of brightness (L<sup>*</sup>), redness (a<sup>*</sup>), and yellowness (b<sup>*</sup>) decreased after blanching except the L<sup>*</sup> value of root at the condition of blanching for 40 s. Upon considering the bacterial cell counts, the proper condition was determined to be 30 s of blanching time. Blanching for 30 s was the optimal pre-treatment for minimizing the quality deterioration of the shepherd`s purse for freezing. Regarding its color change and microbial safety, faster freezing and thawing methods made less changes on the quality of fro-zen samples. Consequently, immersion freezing and radio-frequency thawing were the most effective in minimizing the quality change.

Simultaneous quantification of 60 elements associated with dried red peppers by ICP-OES and ICP-MS for routine analysis

Suel Hye Hur,Jae-Min An,Ji-Hye Lee,Yong-Sik Youn,Hee-Chang Shin,Bo Rim Shin,Ji won Kim,Ho Jin Kim 한국분석과학회 2022 학술대회논문집 Vol.2022 No.5

산업재해보상보험급여와 손해배상의 조정 및 업무상 재해로 인한 손해배상청구에서의 과실상계에 관한 논의

신혜림(Shin, Hye-Rim) 한국사회보장법학회 2016 사회보장법학 Vol.5 No.2

산재법 제80조 제2항에서는 업무상 재해를 입은 근로자가 근로복지공단을 상대로 하는 산재법상 보험급여 청구와 사업주/가해자를 상대로 하는 민사 손해배상 청구를 같이 한 경우, 중복적 보상을 방지하기 위해 피재근로자가 지급받은, 또는 앞으로 지급받을 금액의 한도 내에서 사용자/가해자의 손해배상 책임이 면제되도록 규정하고 있다. 현실에서는 이러한 조정 규정에 의해 피재근로자가 이미 지급받은 급여가 손해배상액에서 ‘전액’ 공제되고 있다. 업무상 재해를 원인으로 한 손해배상청구에서 손해배상액을 산정함에 있어 기지급된 산재보험급여를 모두 공제하는 것은 산재법이 갖는 생활보장적 기능을 제대로 구현하지 못한다는 점에서 문제가 있다. 산재법 제80조 제2항의 ‘금액의 한도 안에서’라는 표현은 ‘이미 지급된 금액을 한도로 모두’라는 적극적인 의미가 아니라 ‘이미 지급된 금액의 일부를 공제할 수 있되 그 금액을 초과해서 공제할 수는 없는’이라는 소극적인 의미로 해석되고 적용되어야 할 것이다. 또한 업무상 재해를 원인으로 하여 피재근로자가 사업주를 상대로 제기한 손해배상 청구에서 피재근로자의 과실비율을 산정함에 있어서는 원․피고 간의 실질적인 비대등성과 현실에서 피재근로자가 부주의할 수밖에 없는 구조적, 근원적 사정을 고려하여야 한다. 그러므로 통상의 과실상계 법리에서 판단기준으로 삼는 ‘약한 부주의’가 아니라, 가해자의 책임을 발생시킬 정도의 주의의무 위반에 준하는 수준의 부주의를 기준으로 삼아 구체적 타당성을 높이고 산재보상과 손해배상의 조정 규정을 둔 본래 취지에 부합하는 과실상계가 이루어지도록 할 필요가 있다. 나아가 손해배상청구에서 기지급된 산재보험급여 공제 및 과실상계를 하는 순서에 있어서도, 현행의 상계후공제설은 뚜렷한 법적 근거가 없고 피재근로자의 생존권 보호에 취약하므로 공제후상계설로의 수정이 요청된다.

HaCaT cells에서 소핵시험을 이용한 positive control 시험

신혜림 ( Shin Hye Rim ),김아름누리 ( Areumnuri Kim ),전경미 ( Kyongmi Chon ),박경훈 ( Kyung-hun Park ),문병철 ( Byeong-chul Moon ),백민경 ( Min Kyoung Paik ) 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-

It has been reported that human keratinocyte cell line (HaCaT cells) are a valuable tool for studying the metabolism of chemicals that penetrate the skin. However, little is known about the genotoxicity and carcinogenicityin HaCaT cellson skin cancer study of carcinogens, such as inorganic arsenic.Therefore, the present studyinvestigated genotoxicity in HaCaT cells by the in vitro micronucleus (vitMN) test. The vitMN test is a method that typically uses cultured human or rodent cells. It provides a comprehensive basis for investigating chromosome damaging potential in vitro because both aneugens and clastogens can be detected. Mitomycin C (MMC; a DNA cross linking agent) and Colchicine (COL; an aneugen) were used as positive controls. One of the most important considerations in the performance of the vitMNtest is ensuring that the cells being scored have completed mitosis during the treatment or the post-treatment incubation period, if one is used. Therefore,following exposure to MMC and COL were harvested after an additional 1.5 to 2.0 times the normal cell cycles. This study was composed of two parts; with cytochalasinB(cyto B) and without cyto B.Positive controls should be used at concentrations expected to give reproducible and detectable increase over negative control which demonstrated the sensitivity of vitMN test. The present study investigated to define the appropriate test concentrations of two positive controlsat significantly increases number of MN in the range of 55±5% cytotoxicity of HaCaT cells. As a result, the appropriate MMC concentrations for short treatment with cyto B and without cyto B were 60 and 10μg/ml, respectively. But 30 μg/ml was selected at COL concentration for short treatment, regardless of cyto B. For continuous treatment, respective 4 and 0.25 μg/ml were as appropriate MMC concentration, and respective 0.2 and 0.1 μg/mlwere as appropriate COL concentrations with cyto B and without cyto B.To the best of our knowledge, this is the first study to establishfor genotoxicity onHaCaT cells in association with carcinogenesis. The present study will provide an important insight into the genotoxicity on HaCaT cells with respect to the genotoxic chemicals.