Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

동의보감 탕액편에 기재된 식이본초의 독성유무에 대한 분류 연구

신호동(Ho-Dong Shin),정종운(Jong-Un Jeong) 대한한의학회 2011 대한한의학회지 Vol.32 No.1

혈관질환 정보관리 시스템

김동익,김덕경,허세호,이병붕,김용신,김은숙,문지영,도영수,신성욱,김동수,김만태,진재욱,김용신 대한혈관외과학회 2002 Vascular Specialist International Vol.18 No.1

As medical technology progresses rapidly, there is a rise in the average age along with the Korean dietary lifestyle becoming more westernized, which leads to an increase in the number of vascular disease patients in Korea. Thus, we need to manage the medical information of a disease systematically in order to diagnose and treat constructively. However, since there has been no standardized method of man agement to date, a great deal of information could not be properly utilized nor studied. Therefore, the departments of Cardiology, Radiology and Neurology of Samsung Seoul Hospital recently got together to develop an information management system called the Vascular Data System. This program was developed to be run on win98 O/S, upper Pentium Ⅲ, and upper 128 MB Memory, and its source code is Dephi 4.0. It was configured for the user to set the configurations as well as do a variety of search and analysis. If this program were to be updated continuously, it may be used extensively as well as in various parts of clinical research activities.

Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

만성 견비통에 대한 동씨침 치료의 무작위 대조군의 임상 연구

김찬영,권나현,신예지,남동우,김건형,김종인,최도영,이윤호,이재동 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2007 東西醫學硏究所 論文集 Vol.2007 No.-

Objectives : To observe the effect of acupuncture treatment in chronic shoulder pain patients. Methods : 36 voluntary patients were randomly assigned to an acupuncture treatment group(E GrouP, n=18) and a control group(C GrouP, n=18). The E Group patients received acupuncture treatment on LI_(15), TE_(14), GB_(21) and Master Dong's acupuncture points, Shin-gwan and Gyun-joong, twice a week for four weeks. The C Group patients received no treatment. All patients in both groups were instructed to practise self exercise in their daily lives. Evaluations were made at baseline and after four weeks of study. The Constant Shoulder Assessment(CSA), Shoulder Pain and Disability Index(SPADI) and the patient's subjective pain was measured by Visual Analogue Scale(VAS). The obtained data was analyzed. Results '. The E Group showed significant(p<0.05) improvement in CSA, SPADI and VAS after four weeks of treatment. The C Group showed significant(p<0.05) improvement in CSA, but the change of SPADI and VAS was insignificant(P>0.05). CSA and SPADI of E Group significantly(p<0.05) improved compared to the C GrouP, but the difference of VAS change in the two groups was insignificant(p>0.05). Conclusions : Four weeks of acupuncture treatment significantly improved CSA, SPADI and VAS. The improvement of CSA and SPADI was significant(p<0.05) compared to untreated patients.

두부손상 환자의 정신과적 합병증에 관한 장애 감정례의 일반 사항에 관한 분석

신석철,왕성근,신윤오,김덕호,김상국,강동숙 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

Authorst ried retrospective analysis to find out general characteristics of the patients undergoing disability evaluation at the department of psychiatry Chungnam National University Hospital from 1986 to 1990. The subjects, who were suffered from psychiatric complications due to head trauma by traffic accident, were 66 patients and they were classified depending on the demographic status, situations of traffic accident, evalaution periods, whether or not perform operation, and rates of evaluated disability and diagnosis. The results were summarized as follows. 1. General background of the subjects were as follows. 1) The frequency order of request were court(59 %) and insurance company(19.7%). 2) The place of accident was more common in large city, common accident vehicle were bus, truck, and cabs. 3) The season of accident were more common in Spring and Autumn. 2. Male(71.2%) was more common than in female and more common in the age group of 5-19 years old(37.9%). The most frequent job of the patients was labour, and the education level was more common in the graduates of elementary school(47.9% ). 3. The interval between accident and evaluation was most frequent in 12-23 months(47.5%), and duration of evaluation was mostly 14 to 21 days. Performed brain operation was 31.8% and non-operated patients was 50.0%. The rates of disability according to the McBride's disability evaluation were mostly belonged to the group of beow 40%(74.1%). 4. The final diagnosis after evaluation were organic personality disorder(45.5%) and dementia(31.8%).

주근단공의 조건에 따른 Root ZX의 정확성 평가

박신영,이동균,황호길 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.2

Objectives: The purpose of this study was to assess the accuracy of Root ZX (J. Morita Corp.) according to the location of major foramen and open apex. Materials and Methods: 81 mandibular premolars with mature apices were selected. After access preparation, 27 teeth were instrumented to simulate open apices. 54 teeth were classified according to location of major foramen under surgical microscope (x16). The file was fixed at the location of apical constriction by Root ZX using glass ionomer cement. The apical 4 mm of the apex was exposed and photo was taken and the distance from file tip to the major foramen was measured by calibrating metal ruler on graph paper. The results were statistically analyzed using ANOVA and Scheffe test at p < 0.05 level. Results: mean distance from file tip to major foramen was 0.308 mm in Tip foramen group (Ⅰ), 0.519 mm in Lateral foramen group (Ⅱ) and 0.932 mm in open apex group (Ⅲ). Root ZX located apical constriction accurately within ± 0.5 mm in group Ⅰ of 85.71%, in group Ⅱ of 59.09%, and in group Ⅲ of 33.33%. There was a statistically significant difference between group Ⅰ and Ⅲ (p < 0.05). Conclusion: Root ZX located apical constriction accurately regardless of location of major foramen. However, Root ZX couldn't find it in open apex. Clinicians have to use a combination of methods to determine an appropriate working length at open apex. It may be more successful than relying on just electronic apes locator. (Restor Dent Endoe 2012;37(2):68-73)

전신경련을 보인 중증 열성 혈소판 감소 증후군에 의한 뇌병증 1 례: 증례보고

전호영, 이혜림, 여민주, 김지선, 신동익, 이성현, 이상수 충북대학교 의학연구소 2016 忠北醫大學術誌 Vol.26 No.2

본문참고

Euonymus alatus 추출뭉의 실험적 간 손상 억제

신숙정,이병용,신동근,이정호 Official Journal of the Korean society for immunol 2001 Immune Network Vol.1 No.3

희생양극을 이용한 자동차 소음기의 음극방식에 관한 연구

신동호,심재주,최윤석,김정구 대한금속재료학회 2004 대한금속·재료학회지 Vol.42 No.12

In order to apply a sacrificial anode cathodic protection system for automotive muffler materials, determination of reliable sacrificial anode and its protection ability were evaluated by laboratory tests in synthetic condensed water. Three kinds of sacrificial anodes were selected: aluminum (AI), zinc (Zn) and magnesium (Mg), and the muffler materials were Al-coated steel and uncoated steel. The corrosion behavior in synthetic condensed water for the muffler materials and sacrificial anodes was investigated by potentiodynamic tests. The cathodic protection ability was evaluated by galvanic corrosion test and weight-loss test. The results of the potentiodynamic test indicated that Al was less suitable for sacrificial anode because it became passive. Mg was also unqualified due to the high dissolution rate. However, Zn was adequate for sacrificial anode because of its low dissolution rate, high stability and suitable potential difference. Results of weight-loss test demonstrated that the corrosion rate of Al-coated steel was reduced from 0.028 to 0.018 mm/year, and that of uncoated steel was 0.08 to 0.002 mm/year by coupling with Zn anode. In addition, the proper area ratio of anode to cathode was 1 : 10. From these results, it can be concluded that Zn anode can protect both the Al-coating with passivation, and steel substrate with low dissolution rate. (Received January 8, 2004)