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- 저자 : Shim Won-Bo (검색결과 406 건)
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Shim, Yoon Bo,Park, Jang Su,Kang, Shin Won,Kim, Andre,Jeong, In Chul 한국생화학회 1978 Vol. No.
The interaction of cytochrome c with binary phospholipid mixtxres was investigated by solid-state ²H- and ^(31)P-NMR. To examine the effect of the interaction on the glycerol backbones, the glycerol moieties of phosphaddylcholine (PC), phosphafidylethanolamine (PE), phosphatidylglycerol (PG), and cardiolipin (CL) were specifically deuterated. On the binding of cytochrome c to the binary mixed bilayers, no changes in the quadrupole splittings of each of the components were observed for the PGPG, PE/CL and PFJ PG 6posomes. In contrast, the splittings of CL decreased on binding of protein to the PGCL liposomes, although those of PC did not change at all. This showed that cytochrome c specifically interacts with CL in PGCL bilayers, and penetrates into the lipid bilayer to some extent so as to perturb the dynamic structure of the glycerol backbone. This is distinctly different from the mode of interaction of cytochrome c with other binary mixed bilayers. In the ^(31)P-NMR spectra, line broadening and a decrease of the chemical shift anisotropy were observed on the binding of cytochrome c for all binary mixed bilayers that were examined. These changes were more significant for the PG CL bilayers. Furthermore, the line broadening is more significant for PC than for CL in PGCL bilayers. Therefore, it can be concluded that with the polar head groups, not only CL but also PC are involved in the interaction with cytochrome c.
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Anti-inflammatory and radical scavenging properties of Verbena officinalis
Shim, Hwan-Ki,Kim, Seong-Yeol,Kim, Bo-Rim,Cho, Jae-Park,Park, Yae-Jeong,Ji, Won-Geun,Cha, Dong-Seok,Jeon, Hoon Kyung Hee Oriental Medicine Research Center 2010 Oriental pharmacy and experimental medicine Vol.10 No.4
Verbena officinalis (Verbenaceae) has been used as herbal medicine or health supplement in both Western and Eastern countries for centuries. In the present study, we examined the anti-inflammatory and antioxidant activities of the methylene chloride fraction of V. officinalis (VMC). To elucidate the anti-inflammatory properties of VMC, we investigated the inhibition effects of nitric oxide production in interferon-gamma (IFN-$\gamma$) and lipopolysaccharide-stimulated mouse peritoneal macrophages. VMC suppressed nitric oxide production, inducible nitric oxide synthase and cyclooxygenase-2 expression dose-dependently without notable cytotoxicity. In various radical scavenging assays, VMC exhibited strong scavenging effect on DPPH radical, superoxide radical, nitric oxide radical and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical. VMC also showed potent reducing power. These findings strongly suggest that VMC may be beneficial in oxidative stress-mediated inflammatory disorders.
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FSR1 is essential for virulence and female fertility in Fusarium verticillioides and F. graminearum.
Shim, Won-Bo,Sagaram, Uma Shankar,Choi, Yoon-E,So, Jinny,Wilkinson, Heather H,Lee, Yin-Won APS Press 2006 Molecular plant-microbe interactions Vol.19 No.7
<P>Fusarium verticillioides (teleomorph Gibberella moniliformis) and F. graminearum (teleomorph G. zeae) are well known to cause devastating diseases on cereal crops. Despite their importance, our understanding of the molecular mechanisms involved in these host-pathogen interactions is limited. The FSR1 locus in F. verticillioides was identified by screening REMI mutants for loss of virulence in maize stalk rot inoculation studies. FSR1 encodes an 823-codon open reading frame interrupted by two introns. The Fsr1 protein shares 60% sequence identity with the Sordaria macrospora Pro11, a multimodular protein with four putative protein-protein binding domains (caveolin-binding domain, coiled-coil structure, calmodulin-binding motif, and seven-WD40 repeats), which plays a regulatory role in cell differentiation and ascocarp development. Our data demonstrate that FSR1 is essential for female fertility and virulence in F. verticillioides. Significantly, targeted disruption of the FSR1 ortholog in F. graminearum (FgFSR1) reduced virulence on barley and deterred perithecia formation. Cross-complementation experiments demonstrated that the gene function is conserved in the two Fusarium species. FSR1 is expressed constitutively, and we hypothesize that Fsr1 regulates virulence by acting as a scaffold for a signal transduction pathway. A survey of available genome databases indicates Fsr1 homologs are present in a number of filamentous fungi and animal systems but not in budding yeast or plants. A maximum likelihood analysis of this gene family reveals well-supported monophyletic clades associated with fungi and animals.</P>
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Shim, Won-Bo,Je, Gil-Soo,Kim, Kyeongyeol,Mtenga, Adelard B.,Lee, Won-Gyeong,Song, Jeong-Un,Chung, Duck-Hwa,Yoon, Yohan Elsevier 2012 Radiation physics and chemistry Vol.81 No.5
<P><B>Abstract</B></P><P>This study evaluated effect of gamma irradiation on survival of <I>Salmonella Typhimurium</I> and <I>Staphylococcus aureus</I> on lettuce and damage of cell envelope. <I>S.</I> Typhimurium and <I>S. aureus</I> were inoculated on red leaf lettuce, and they were irradiated at 0, 0.5, 1, 1.5, 2, 2.5, and 3kGy, and the samples were then stored at 7 and 25°C for 7 days. Survival of <I>S.</I> Typhimurium and <I>S. aureus</I> were enumerated on xylose lysine deoxycholate agar and Baird–Parker agar, respectively. <I>D</I><SUB>10</SUB> value (dose required to reduce 1log CFU/leaf) was calculated, and kinetic parameters (maximum specific growth rate; <I>μ</I><SUB>max</SUB> and lag phase duration; LPD) were calculated by the modified Gompertz model. In addition, cell envelope damage of the pathogens was observed by scanning electron microscope (SEM) and transmission electron microscope (TEM). <I>D</I><SUB>10</SUB> values were 0.35 and 0.33kGy for <I>S.</I> Typhimurium and <I>S. aureus</I>, respectively. During storage at 7°C, <I>S.</I> Typhimurium and <I>S. aureus</I> had significant (<I>P</I><0.05) growth only on non-irradiated samples up to about 2.5 and 4log CFU/leaf at 0.42 and 1.28log CFU/leaf/day of <I>μ</I><SUB>max</SUB>, respectively. At 25°C, cell counts of <I>S.</I> Typhimurium and <I>S. aureus</I> on the samples irradiated at 0 and 0.5kGy increased (<I>P</I><0.05) up to 3–6log CFU/leaf. The <I>μ</I><SUB>max</SUB> of both pathogens were higher in 0kGy <I>(</I>1.08–2.27log CFU/leaf/day) and 0.5kGy (0.58–0.92log CFU/leaf/day), and LPDs ranged from 1.53 to 3.14 day. SEM and TEM observations showed that cells irradiated at 1.5 and 3kGy showed disrupted cell membrane. These results indicate that gamma irradiation could be a useful decontamination technology to improve food safety of lettuce by destroying cells of <I>S.</I> Typhimurium and <I>S. aureus</I>.</P> <P><B>Highlights</B></P><P>► Low dose of gamma irradiation destroyed cell envelope of the pathogens. ► Gamma irradiation decreased cell counts of the pathogens on lettuce. ► Gamma irradiation could be useful in improving food safety of lettuce.</P>
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Shim, Doo-Bo,Park, Eun-Sil,Sim, Gyu-Jin,Lee, Ji-Young,Kang, Ju-Hwan,Yoo, Hyun-Joo,Choi, Yeon-Jin,Lee, Young-Mee,Lee, Sang-Yeol,Kim, Min-Gab,Kang, Da-Won,Jung, Eun-Jung,Kang, Kee-Ryeon The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
Eukaryotic translation initiation factor 5A (eIF5A) is the only hypusine-containing protein, which is formed by deoxyhypusine synthase (DHS) and deoxyhypusine hydroxylase (DOHH). DOHH is a novel metalloenzyme with HEAT [named for human huntingtin (H), elongation factor 3 (E), a subunit of protein phosphatase 2A (A), and the target of rapamycin (T)]-repeat motifs. Inspite of much progress in determining the roles of iron-containing DOHH holoenzyme as an eIF5A hydroxylase, little is known about iron-free apoenzyme of DOHH under certain stress conditions. For this purpose, we compared cell growth in two yeast strains subjected to oxidative damage. Thus, the existence of more viable cells in the Saccharomyces cerevisiae BY4743 (parental yeast) strain than in the DOHH- strain after $H_2O_2$ treatment indicates the importance of DOHH in protecting yeast cells against oxidative stress. To identify endogenous target proteins influenced by DOHH under oxidative damage, proteomic analysis was applied to the two yeast strains. Of these proteins, the oxidized form of peroxiredoxin I (PrxI) was concomitantly up-regulated in both strains under $H_2O_2$ treatment. Two-dimensional electrophoresis (DE) followed by immunoblot analysis shows that the recovery of the oxidized PrxI to the reduced enzyme under $H_2O_2$ treatment was found to be much slower in the DOHH- strain than in the parental strain. Based on the results, we discovered a possible interaction between DOHH and PrxI by immunoprecipitation/immunoblotting in yeast under oxidative stress. Taken together, these results suggest that DOHH might be a candidate protein for protection of yeast cells against oxidative stress in conjunction with PrxI.
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Shim, Jun-Bo,Klochkova, Tatyana A.,Han, Jong-Won,Kim, Gwang-Hoon,Yoo, Yeong-Du,Jeong, Hae-Jin The Korean Society of Phycology 2011 ALGAE Vol.26 No.1
Protein profiles of a common mixotrophic dinoflagellate, Prorocentrum micans, growing autotrophically and mixotrophically (fed on the cryptophyte Rhodomonas salina) were compared using two-dimensional gel electrophoresis (2-DE) to determine if they vary in different trophic modes. Approximately 2.3% of the detected proteins were differentially expressed in the different trophic modes. Twelve proteins observed only in the mixotrophic condition had lower pI value (<5) than the fifteen proteins observed only in the autotrophic condition (>5). When the internal amino acid sequences of five selected proteins differentially expressed between autotrophic and mixotrophic conditions were analyzed using matrix-assisted laser desorption time-of-flight (MALDI-TOF) mass spectrometry, two proteins that were specifically expressed in the autotrophic condition showed homology to glyceraldehyde-3-phosphatase dehydrogenase (GAPDH) and a bacterial catalase. Three mixotrophy-specific proteins showed homology to certain hypothetical proteins from an insect and bacteria. These results suggested the presence of certain gene groups that are switched on and off according to the trophic mode of P. micans.
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Bo Kyong Kim,임도훈,Won Park,Joungho Han,Keunchil Park,Kwan Min Kim,김진국,Young Mog Shim,Yong Chan Ahn,Kyoung Ju Kim 대한암학회 2003 Cancer Research and Treatment Vol.35 No.6
Purpose: To evaluate the prognostic implication of histopathologic findings on the surgical specimens of N2 positive stage IIIA non-small cell lung cancer (NSCLC) patients who were treated with preoperative concurrent radiochemotherapy (CRCT) and surgery.Materials and Methods: From May 1997 to April 2000, 48 patients with N2 positive stage IIIA NSCLC were treated with preoperative CRCT and surgery. Retrospective analyses were performed on 33 patients who underwent surgical resection. The thoracic radiation therapy (TRT) dose was 45 Gy over 5 weeks with a 1.8 Gy daily fraction using 10 MV X-rays. Chemotherapy consisted of two cycles of intravenous cisplatin (100 mg/m2, on days 1 and 29) and oral etoposide (50 mg/m2/day, on days 1?? 14 and 29??42), concurrently delivered with TRT. Surgery was performed around 4 weeks of the completion of CRCT. The median follow up was 18 months. The histopathologic findings, including the proportions of viable tumor cells, fibrosis, and necrosis, as well as the tumor and nodal statuses on the surgical specimens following the preoperative CRCT, were analyzed.Results: The 3-year overall survival, disease-free survival, and local control rates were 46.1%, 49.5%, and 85.5%, respectively. Post-surgical stages decreased in 18 patients (54.5%), including 3 pathologic complete responses, were unchanged in 13 (39.4%), and increased in two (6.1%). On univariate analyses, the low proportion of the viable tumor cells was the only factor favorably affecting the overall survival rate (p=0.0386), and the histologic type of squamous cell carcinoma was a favorable factor affecting disease free survival rate (p=0.0452). On multivariate analyses, however, no factor affected the overall survival, disease free survival, or local control rates.Conclusion: The histopathologic findings of the proportion of viable tumor cells, fibrosis, and necrosis on the surgical specimens following preoperative CRCT had few prognostic implications on uni-and multi-variate analyses.Furthermore, the primary tumor and nodal responses to preoperative CRCT did not influence the outcomes. Longer- term follow-up with a larger number of patients, however, is awaited. (Cancer Research and Treatment 2003; 35:497-501)
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( Bo Young Lee ),( Jae Young Jang ),( Soung Won Jeong ),( Gene Hyun Bok ),( Jeong Ho Ham ),( Joo Young Cho ),( Joon Seong Lee ),( Chan Sup Shim ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2011 Gut and Liver Vol.5 No.2
We report two cases of adrenal abscesses that occurred following a Histoacryl(R) (N-butyl-2-cyanocrylate) injection for variceal bleeding. Patients had been diagnosed with alcoholic liver cirrhosis and gastric varices bleeding and received a Histoacryl(R) injection for the variceal bleeding. Patients had fever and abdominal tenderness and were diagnosed with an adrenal abscess at 2 months following the Histoacryl(R) injection. One patient received open drainage and the other underwent percutaneous drainage. When a patient has previously been injected with Histoacryl(R) for the treatment of variceal bleeding and presents with fever, an evaluation for an unusual complication such as adrenal abscess is recommended. (Gut Liver 2011;5:242-244)
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