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Predicting BOG rate in cryogenic containers at different liquid levels based on a single test
Zheng-Qing LI,Xiaojun Wang,Shengsheng Yang 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.9
As the boil-off gas (BOG) rate is tested to evaluate the thermal insulation performance of cryogenic containers at a 90 % liquid level in standard. This study aims to predict the BOG rate of cryogenic containers at different liquid levels based on a single test. If the BOGs at a 90 % liquid level can be evaluated based on the BOG test at a low liquid level, the large amount of working medium can be saved to reduce the test cost and the equilibration time can be shortened to improve the test efficiency. In this study, the QHALW (heat absorbed by the liquid from its corresponding outer wall) and QHTVL (heat transferred from the corresponding wall of the vapor to the liquid) are analysed to establish the energy equation of the liquid in the BOG test, indicating latent heat of BOG in cryogenic containers includes the QHALW and QHALW. Therefore, the equation between the latent heat of BOG and the two parts of heat isbuilt to predict BOGs at different liquid levels with the tested BOG. The maximum error of the experimental results is not more than 7.0 %, which shows that the BOG is successfully predicted at different liquid levels with a single-tested BOG.
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Shoumei Zhang,Li Shuai,Dong Wang,Tingting Huang,Shengsheng Yang,Mingyong Miao,Fang Liu,Jiajun Xu 한국뇌신경과학회 2020 Experimental Neurobiology Vol.29 No.3
Provirus integration site Moloney murine leukemia virus (Pim-1) is a proto-oncogene reported to be associated with cell proliferation, differentiation and survival. This study was to explore the neuroprotective role of Pim-1 in a rat model subjected to optic nerve crush (ONC), and discuss its related molecules in improving the intrinsic regeneration ability of retinal ganglion cells (RGCs). Immunofluorescence staining showed that AAV2- Pim-1 infected 71% RGCs and some amacrine cells in the retina. Real-time PCR and Western blotting showed that retina infection with AAV2- Pim-1 up-regulated the Pim-1 mRNA and protein expressions compared with AAV2-GFP group. Hematoxylin-Eosin (HE) staining, γ-synuclein immunohistochemistry, Cholera toxin B (CTB) tracing and TUNEL showed that RGCs transduction with AAV2-Pim-1 prior to ONC promoted the survival of damaged RGCs and decreased cell apoptosis. RITC anterograde labeling showed that Pim-1 overexpression increased axon regeneration and promoted the recovery of visual function by pupillary light reflex and flash visual evoked potential. Western blotting showed that Pim- 1 overexpression up-regulated the expression of Stat3, p-Stat3, Akt1, p-Akt1, Akt2 and p-Akt2, as well as βIII-tubulin, GAP-43 and 4E-BP1, and downregulated the expression of SOCS1 and SOCS3, Cleaved caspase 3, Bad and Bax. These results demonstrate that Pim-1 exerted a neuroprotective effect by promoting nerve regeneration and functional recovery of RGCs. In addition, it enhanced the intrinsic regeneration capacity of RGCs after ONC by activating Stat3, Akt1 and Akt2 pathways, and inhibiting the mitochondrial apoptosis pathways. These findings suggest that Pim-1 may prove to be a potential therapeutic target for the clinical treatment of optic nerve injury
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Ultrathin Biocompatible Electrospun Fiber Films for Self-Powered Human Motion Sensor
Gengrui Zhao,Shaobo Gong,Honggang Wang,Junfang Ren,Na Wang,Yawen Yang,Gui Gao,Shengsheng Chen,Linlin Li 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.8 No.3
In recent years, triboelectric nanogenerator (TENG) has been proven to be an effective and simple solution for self-powered wearable sensing, and a wide variety of materials and methods have been applied to TENG based sensors. In this study, an ultrathin fiber film with nanoporous brain-like structure (BUF) was obtained by cold-pressing the electrospun biocompatible fiber mats, and a wearable TENG sensor based on the BUF films that could sensitively detect human motion was demonstrated. This simply designed self-powered sensor was composed of Kapton as the shell and ultrathin, biocompatible poly L-lactic acid and ethyl cellulose (EC) fiber films as the triboelectric layers, prepared by convenient electrospinning method and cold-pressing. Compared to as-spun fiber mats, cold-pressed fiber membranes not only acquire overall compact structure but also remained porous surface morphology, which improved the triboelectric outputs and operational stability of TENG. Three TENGs were fabricated to evaluate the effects of different triboelectric layers on triboelectric outputs, and the BUF-TENG had the maximum triboelectric outputs (19 V, 630 nA at 3.5 Hz) and longer life than as-spun fiber mats based TENG. The BUF-TENG had linear relationship between triboelectric outputs and frequency, and sensitivities of 32.4, 0.94 and 0.22 V Hz−1 in the range of 0.1–0.35, 0.35–2 and 2–3.5 Hz, respectively. Moreover, a self-powered sensor based on the BUF-TENG was proposed to sensitively detect human walking state and limbs motion. With convenient preparation process and biocompatible materials, the BUF-TENG sensor could be a potential self-powered wearable and portable sensor in future health care and monitoring field.
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Huimin Zhao,Junyu Nie,Xiangxing Zhu,Yangqing Lu,Xingwei Liang,Huiyan Xu,Xiaogan Yang,Yunkai Zhang,Kehuan Lu,Shengsheng Lu 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.5
In this study, we attempted to establish a culture system for in vitro spermatogenesis from spermatogonial stem cells (SSCs) of Bama mini-pig. Dissociated testicular cells from 1-month-old pigs were co-cultured to mimic in vivo spermatogenesis. The testicular cells were seeded in minimum essential medium alpha (a-MEM) supplemented with Knockout serum replacement (KSR). Three-dimensional colonies formed after 10 days of culture. The colonies showed positive staining for SSC-associated markers such as UCHL1, PLZF, THY1, OCT4, Dolichos biflorus agglutinin, and alkaline phosphatase. Induction of SSCs was performed in a-MEM + KSR supplemented with retinoic acid, bone morphogenetic protein 4, activin A, follicle-stimulating hormone, or testosterone. The results showed that STRA8, DMC1, PRM1, and TNP1 were upregulated significantly in the colonies after induction compared to that in testis from 1-month-old pigs, while expression levels of those genes were significantly low compared to those in 2-month-old testis. However, upregulation of ACROSIN was not significant. Replacement of a-MEM and KSR with Iscove’s modified Dulbecco’s medium and fetal bovine serum did not upregulate expression of these genes significantly. These results indicate that SSCs of Bama mini-pig could undergo differentiation and develop to a post-meiotic stage in α-MEM supplemented with KSR and induction factors.
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