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A Mechanogenetic Toolkit for Interrogating Cell Signaling in Space and Time
Seo, Daeha,Southard, Kaden M.,Kim, Ji-wook,Lee, Hyun Jung,Farlow, Justin,Lee, Jung-uk,Litt, David B.,Haas, Thomas,Alivisatos, A. Paul,Cheon, Jinwoo,Gartner, Zev J.,Jun, Young-wook Cell Press 2016 Cell Vol. No.
<P><B>Summary</B></P> <P>Tools capable of imaging and perturbing mechanical signaling pathways with fine spatiotemporal resolution have been elusive, despite their importance in diverse cellular processes. The challenge in developing a mechanogenetic toolkit (i.e., selective and quantitative activation of genetically encoded mechanoreceptors) stems from the fact that many mechanically activated processes are localized in space and time yet additionally require mechanical loading to become activated. To address this challenge, we synthesized magnetoplasmonic nanoparticles that can image, localize, and mechanically load targeted proteins with high spatiotemporal resolution. We demonstrate their utility by investigating the cell-surface activation of two mechanoreceptors: Notch and vascular endothelial cadherin (VE-cadherin). By measuring cellular responses to a spectrum of spatial, chemical, temporal, and mechanical inputs at the single-molecule and single-cell levels, we reveal how spatial segregation and mechanical force cooperate to direct receptor activation dynamics. This generalizable technique can be used to control and understand diverse mechanosensitive processes in cell signaling.</P> <P><B>Video Abstract</B></P> <P>Display Omitted</P> <P><B>Highlights</B></P> <P> <UL> <LI> Development of a mechanogenetic single-cell perturbation approach </LI> <LI> Interrogation of the spatial, chemical, and mechanical responses of Notch receptors </LI> <LI> Identification of the roles of spatial and mechanical cues on VE-cadherin signaling </LI> <LI> Spatiotemporal and quantitative control of single-cell transcription by nanoprobes </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>
Shape auxiliary approach for carboxylate-functionalized gold nanocrystals
Seo, Daeha,Yoo, Choong Il,Chung, Bong Hyun,Chung, Im Sik,Song, Hyunjoon Royal Society of Chemistry 2009 Chemical communications Vol.2009 No.10
<P>A shape auxiliary approach for the synthesis of shape-controlled gold nanocrystals with functional moieties is established; carboxylate-functionalized gold polyhedra were successfully synthesized in a one-pot reaction in the presence of poly(dimethylaminoethyl methacrylate), which contains the dimethylaminoethyl group as a shape auxiliary.</P> <P>Graphic Abstract</P><P>Carboxylate-functionalized gold polyhedra were successfully synthesized in a one-pot reaction in the presence of poly(dimethylaminoethyl methacrylate), which contains the dimethylaminoethyl group as a shape auxiliary. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b820220f'> </P>
Directed Surface Overgrowth and Morphology Control of Polyhedral Gold Nanocrystals
Seo, Daeha,Yoo, Choong Il,Park, Ji Chan,Park, Seung Min,Ryu, Seol,Song, Hyunjoon WILEY-VCH Verlag 2008 Angewandte Chemie Vol.47 No.4
<B>Graphic Abstract</B> <P>Shapeshifters: The shape and size of gold nanocrystals were controlled simultaneously through directed surface overgrowth from polyhedral and spherical seeds of different sizes. The resulting cubes, cuboctahedra, and octahedra (shown as SEM images for growth from spherical seeds of two sizes) exhibited characteristic optical properties in the visible range, which were analyzed by discrete dipole approximation calculations. <img src='wiley_img/14337851-2008-47-4-ANIE200704094-content.gif' alt='wiley_img/14337851-2008-47-4-ANIE200704094-content'> </P>
Shape Evolution and Gram-Scale Synthesis of Gold@Silver Core–Shell Nanopolyhedrons
Park, Garam,Seo, Daeha,Jung, Jongwook,Ryu, Seol,Song, Hyunjoon American Chemical Society 2011 The Journal of Physical Chemistry Part C Vol. No.
<P>Au@Ag heterometal core–shell nanoparticles with distinct polyhedral shapes were successfully synthesized by a seed-mediated polyol process on a gram scale. The addition of the silver precursor solution led to continuous shape evolution from gold seeds to silver spheres, cuboctahedrons, and cubes, respectively. Silver nanocubes were also grown by selective edge growth on gold cuboctahedral seeds. The overgrowth of silver shells could precisely tune the optical properties of the core–shell polyhedrons. Interestingly, a large red-shift of the extinction peak from that of the gold seeds was observed in the Au cuboctahedron@Ag cubes, reflecting a combination of composition and shape effects.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2011/jpccck.2011.115.issue-19/jp200338p/production/images/medium/jp-2011-00338p_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jp200338p'>ACS Electronic Supporting Info</A></P>
Park, Garam,Seo, Daeha,Chung, Im Sik,Song, Hyunjoon American Chemical Society 2013 Langmuir Vol.29 No.44
<P>Gold nanoparticles with suitable surface functionalities have been widely used as a versatile nanobioplatform. However, functionalized gold nanoparticles using thiol-terminated ligands have a tendency to aggregate, particularly in many enzymatic reaction buffers containing biological thiols, because of ligand exchange reactions. In the present study, we developed a one-step synthesis of poly(ethylene glycol) (PEG)ylated gold nanoparticles using poly(dimethylaminoethyl methacrylate) (PDMAEMA) in PEG as a polyol solvent. Because of the chelate effect of polymeric functionalities on the gold surface, the resulting PEGylated gold nanoparticles (Au@P-PEG) are very stable under the extreme conditions at which the thiol-monolayer-protected gold nanoparticles are easily coagulated. Using the solvent mixture of PEG and ethylene glycol (EG) and subsequent hydrolysis, gold nanoparticles bearing mixed functionalities of PEG and carboxylate are generated. The resulting particles exhibit selective adsorption of positively charged chymotrypsin (ChT) without nonselective adsorption of bovine serum albumin (BSA). The present nanoparticle system has many advantages, including high stability, simple one-step synthesis, biocompatibility, and excellent binding specificity; thus, this system can be used as a versatile platform for potential bio-related applications, such as separation, sensing, imaging, and assays.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/langd5/2013/langd5.2013.29.issue-44/la402315a/production/images/medium/la-2013-02315a_0013.gif'></P>
Park, Garam,Lee, Chanhyoung,Seo, Daeha,Song, Hyunjoon American Chemical Society 2012 Langmuir Vol.28 No.24
<P>In the present study, we demonstrate the precise tuning of surface plasmon resonance over the full visible range by compositional variation of the nanoparticles. The addition of sulfide ions into the Au@Ag core–shell nanocubes generates stable Au@Ag/Ag<SUB>2</SUB>S core–shell nanoparticles at room temperature, and the plasmon extinction maximum shifts to the longer wavelength covering the entire visible range of 500–750 nm. Based on the optical property, the Au@Ag core–shell nanocubes are employed as a colorimetric sensing framework for sulfide detection in water. The detection limit is measured to be 10 ppb by UV–vis spectroscopy and 200 ppb by naked eyes. Such nanoparticles would be useful for decoration and sensing purposes, due to their precise color tunability and high stability.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/langd5/2012/langd5.2012.28.issue-24/la300154x/production/images/medium/la-2012-00154x_0001.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/la300154x'>ACS Electronic Supporting Info</A></P>