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Sarita Paudel,박제원,이주호,윤여준,송재경 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.1
Two putative ABC transporter genes pdmR1and pdmR2 are found in the biosynthetic gene cluster of pradimicin. pdmR1 showed a hydrophilic profile with similarity to the daunorubicin-resistant ABC transporter ATP-binding protein family, a characteristic ABC transporter. pdmR2 codes for hydrophobic polypeptides showing a high degree of similarity to several ABC-type multidrug transport systems. To assess the possible roles of these genes in pradimicin biosynthesis, pdmR1 and pdmR2 were introduced into Actinomadura hibisca P-1752. The recombinant strains Actinomadura hibisca SP1, SP2, and SP3 showed longer life spans compared to the wild-type A. hibisca. Pradimicin production was increased by 3.5, 2.4,and 5.4-fold at 8 days in A. hibisca SP1, SP2 and SP3,respectively, compared to that of parental strain. The higher transcriptional level of pdmR1 and pdmR2 genes in the pdmR1 and pdmR2 harboring strains compared to the wild type was consistent with the enhanced production. The plasmids pSP1, pSP2, and pSP3 were expressed in Streptomyces venezulae and S. lividians, which are sensitive to pradimicin. The transformants acquired higher resistance to pradimicin than the wild-type Streptomyces strains suggesting that pdmR1 and pdmR2 acts as a set of resistance genes in A. hibisca to pump out pradimicin produced throughout the cell.
Sarita Paudel,Hei Chan Lee,Jae Kyung Sohng 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1
α-Glucosidase of Thermotoga maritima belongs to family 4 of the Glycosyl hydrolases. The enzyme α-glucosidase has the unusual property of requiring NAD+, divalent metal cations Mn2+ and reducing conditions (DTT) for activity. The gene aglS encoding 459 amino acid with 51 kDa was expressed under the control of the T7 promoter in Escherichia coli. It was found soluble form and thermo-stable up to 70oC. It showed hydrolyzing activity towards para-nitrophenyl-α-D-glucoside (pNP-α-Glc). The transglycosylation activity was tested using maltoseand sucrose as donor and cyclodextrin as acceptor. The molecular mass of cyclodextrin containing two glycosyl moieties is seen in ESI/MS when maltose and sucrose was used as donor. The further analysis is yet to be confirmed.