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Study on Leaf Production of Angelica acutiloba by Mulching with Polyethylene Film
Choi,Seong-Kyu,Yun,Kyeong-Won,Chon,Sang-Uk,Seo,Young-Nam,Seo,Kyoung-sun,Kwon,Byung-Sun 한국자원식물학회 2003 Plant Resources Vol.6 No.3
This study was conducted to produce effectively more leaves of Angelica acutiloba through year-round production system by using various mulching materials and polyethylene(PE) tunnel. Soil temperature by covering treatment was higher in PE mulching and PE tunnel than in rice straw mulching, especially showing the highest temperature in PE tunnel from April through May. Water content of soil was higher in PE mulching than in non-mulching. Leaf production of Angelica acutiloba through year-round production showed the highest in PE tunnel and root production in PE mulching. These results suggest that leaf production of Angelica acutiloba can be maximized through PE tunnel cultivation system.
Effect of Activated Charcoal on Growth of Zingiber mioga ROSC
Choi,Seong-Kyu,Yun,Kyeong-Won,Chon,Sang-Uk,Seo,Young-Nam,Seo,Kyoung-Sun,Kwon,Byung-Sun 한국자원식물학회 2004 Plant Resources Vol.7 No.2
Activate charcoal (AC) can be utilized as a soil conditioner in agricultural crop areas. This study was conducted to investigate the effect of AC on growth and yield of Zingiber mioga ROSC as affected by different amounts of AC. The results obtained are summarized as follows. Growth characteristics including plant height and leaf length were the highest when activated charcoal added with 10-30%, suggesting that optimum amount of activated charcoal was ranged from 10 to 30%. Growth and enlargement of the root were improved by 10% AC with higher rhizome length and weight.
Effect of Activated Charcoal on Growth of Curcuma longa Linne
Choi,Seong-Kyu,Yum,Kyeong-Won,Chon,Sang-Uk 한국자원식물학회 2003 Plant Resources Vol.6 No.3
This study was conducted to investigate the effect of Activated charcoal (AC) for stimulating growth and productivity of Curcuma longa Linne as affected by different concentrations of AC. Growth characteristics such as plant height and leaf length were the highest with the optimum amount of activated charcoal ranged from 10 to 30%. Root growth was elongated by the addition of 30% AC with the highest rhizome length and weight.
A Basic Study on Leaf and Stem Production of Angelica acutiloba
Choi,Seong-Kyu,Yun,Kyeong-won,Chon,Sang-Uk,Lee,Jong-Ill,Seo,Young-Nam,Seo,Kyoung-Sun,Choi,Kyeong-Ju 한국자원식물학회 2004 Plant Resources Vol.7 No.3
This study was conducted to develop effective production system in greenhouse for leaves and stems of Angelica acutiloba by using connect pots in 2003 and 2004. Seed germination rate and plant biomass of Angelica acutiloba collected in 2004 were higher than those harvested in 2003. Germination rate of Angelica acutiloba seeds collected in 2003 was 10%, while germination rate of seeds collected in 2004 was above 90%. Especially, plant growth and yield of Angelica acutiloba grown in connect pots sized with 4×4×5cm(length×width×height) were the highest. These results indicate that leaf and stem production of Angelica acutiloba can be improved by using connect pots and optimizing seed collecting time in greenhouse.
Effect of Storage Condition on Yield and Quality of Angelica acutiloba Radix
Choi,Seong-kyu,Yun,Kyeong-Won,Chon,Sang-Uk,Seo,Young-nam 한국자원식물학회 2002 Plant Resources Vol.5 No.1
To develop an optimal storage method of root of Angelica acutiloba Radix, which has been grown as major herbal medicine material to be cultivated, root of Angelica acutiloba Radix, was stored for 10 months with different packing materials and sealing methods. The loss in dry weight as influenced by packing materials and sealing methods was the lowest at vacuum packing and followed by complete sealing methods with transparent polyethylene film. The ratio of root rot during the storage period was not significantly different between packing materials but was significantly different between sealing conditions. In conclusion, vacuum packing and complete sealing with polyethylene film appears to be most optimal for storage of Angelica acutiloba Radix.
Proteomic Characterization of the Outer Membrane Vesicle of <i>Pseudomonas putida</i> KT2440
Choi, Chi-Won,Park, Edmond Changkyun,Yun, Sung Ho,Lee, Sang-Yeop,Lee, Yeol Gyun,Hong, Yeonhee,Park, Kyeong Ryang,Kim, Sang-Hyun,Kim, Gun-Hwa,Kim, Seung Il American Chemical Society 2014 JOURNAL OF PROTEOME RESEARCH Vol.13 No.10
<P>Outer membrane vesicles (OMVs) are produced by various pathogenic Gram-negative bacteria such as <I>Escherichia coli</I>, <I>Pseudomonas aeruginosa</I>, and <I>Acinetobacter baumannii</I>. In this study, we isolated OMVs from a representative soil bacterium, <I>Pseudomonas putida</I> KT2440, which has a biodegradative activity toward various aromatic compounds. Proteomic analysis identified the outer membrane proteins (OMPs) OprC, OprD, OprE, OprF, OprH, OprG, and OprW as major components of the OMV of <I>P. putida</I> KT2440. The production of OMVs was dependent on the nutrient availability in the culture media, and the up- or down-regulation of specific OMPs was observed according to the culture conditions. In particular, porins (e.g., benzoate-specific porin, BenF-like porin) and enzymes (e.g., catechol 1,2-dioxygenase, benzoate dioxygenase) for benzoate degradation were uniquely found in OMVs prepared from <I>P. putida</I> KT2440 that were cultured in media containing benzoate as the energy source. OMVs of <I>P. putida</I> KT2440 showed low pathological activity toward cultured cells that originated from human lung cells, which suggests their potential as adjuvants or OMV vaccine carriers. Our results suggest that the protein composition of the OMVs of <I>P. putida</I> KT2440 reflects the characteristics of the total proteome of <I>P. putida</I> KT2440.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jprobs/2014/jprobs.2014.13.issue-10/pr500411d/production/images/medium/pr-2014-00411d_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/pr500411d'>ACS Electronic Supporting Info</A></P>
Choi, Jaewon,Hwang, Yu Kyeong,Sung, Ki Woong,Lee, Soo Hyun,Yoo, Keon Hee,Jung, Hye Lim,Koo, Hong Hoe,Kim, Hee-Jin,Kang, Hyong Jin,Shin, Hee Young,Ahn, Hyo Seop American Society of Hematology 2007 Blood Vol.109 No.2
<B>Abstract</B><P>Livin, a member of the inhibitor of apoptosis proteins, has been considered to be a poor prognostic marker in malignancies. However, little is known about the clinical relevance of Livin expression in childhood acute lymphoblastic leukemia (ALL). In this study, the expression of Livin was analyzed in 222 patients with childhood ALL using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to investigate a possible association with the clinical features at diagnosis and treatment outcomes. Both Livin expression rates and expression levels were higher in patients with favorable prognostic factors. The expression rate was also higher in patients with a favorable day 7 bone marrow response to induction chemotherapy (P < .001). The Livin expression was related to the absence of relapse (P < .001). Similarly, the relapse-free survival rate (± 95% CI) was higher in patients with Livin expression than in patients without Livin expression (97.9% ± 4.0% versus 64.9% ± 11.8%, P < .001). Multivariate analysis for relapse-free survival demonstrated that Livin expression was an independent favorable prognostic factor in childhood ALL (P = .049). This study suggests that Livin expression is a novel prognostic marker in childhood ALL and thus needs to be incorporated into the patient stratification and treatment protocols.</P>
Choi, Jaewon,Hwang, Yu Kyeong,Choi, Young Jin,Yoo, Ki Eun,Kim, Jeong Han,Nam, Seok Jin,Yang, Jung Hyun,Lee, Sang Jin,Yoo, Keon Hee,Sung, Ki Woong,Koo, Hong Hoe,Im, Young-Hyuck The Korean Academy of Medical Sciences 2007 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.22//SUP No.-
<P>Neuronal apoptosis inhibitory protein (NAIP) is a recently identified inhibitor of apoptosis protein. However, the clinical relevance of NAIP expression is not completely understood. In an attempt to determine the clinical relevance of NAIP expression in breast cancer, the levels of NAIP and survivin expression were measured in 117 breast cancer samples and 10 normal breast tissues using quantitative reverse-transcriptase-polymerase chain reaction. While there was no evidence of NAIP expression in the normal breast tissue, NAIP was expressed in all breast cancer samples. The level of NAIP expression in breast cancer was significantly higher (257 times) than in the universal tumor control. There was a strong correlation between the level of NAIP expression and the level of survivin expression (<I>p</I>=0.001). The level of NAIP expression in patients with a large tumor (≥T2) and patients with an unfavorable histology (nuclear grade III) was significantly higher than in those patients with a small tumor (T1) and patients with a favorable histology (nuclear grade I, II) (<I>p</I>=0.026 and <I>p</I>=0.050, respectively). Although the level of NAIP expression was higher in patients with other unfavorable prognostic factors, it was not significant. The three-year relapse-free survival rate was not significantly the patients showing high NAIP expression and patients showing low NAIP expression (86.47±4.79% vs. 78.74±6.57%). Further studies should include the expressions of NAIP in a larger number of patients and for a longer period of follow-up to evaluate correlation with metastasis and treatment outcome. In conclusion, NAIP is overexpressed in breast cancer patients with unfavorable clinical features such as stage and tumor size, suggesting that NAIP would play a role in the disease manifestation.</P>