http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
이춘환,김경숙,Robert t . Ross ( Choon Hwan Lee,Kyung Sook Kim,Robert T . Ross ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.4
The information contained in vivo fluorescence spectra of biological specimens is often obscured by severe overlapping of component spectra. Fluorescence intensity is separately linear in functions of excitation wavelength, emission wavelength, and any chemical treatment which alters overall fluorescence yield. When data are collected for several values of each of these three independent variables, strongly overlapping spectra can be resolved without the use of any a priori information about their shapes. Using concentration of a fluorescence quencher, potassium iodide, as a third variable, a mixture of rhodamine 6G, rhodamine B, and sulforhodamine 101 was used as a test system; the spectra resolved agreed closely with the spectra of the pure dyes. Previously published applications of trilinear analysis to luminescence spectroscopy have required either an instrument capable of time resolution or multiple specimens with varying relative concentrations of fluorophores; use of quencher concentration as a variable permits use of the method with a steady-state fluorometer and without changing fluorophore concentrations. Requiring that the spectra deduced be non-negative can increase the accuracy of the results.