D-RADA16-RGD-Reinforced Nano-Hydroxyapatite/Polyamide 66 Ternary Biomaterial for Bone Formation

Bo Qiao,Bin He,Ao Zhou,Yuling Li,Xiaojun Chen,Qiming Yang,Beike Chen,Bo Qiao,Dianming Jiang 한국조직공학과 재생의학회 2019 조직공학과 재생의학 Vol.16 No.2

BACKGROUND: Nano-hydroxyapatite/polyamide 66 (nHA/PA66) is a composite used widely in the repair of bone defects. However, this material is insufficient bioactivity. In contrast, D-RADA16-RGD self-assembling peptide (DRADA16- RGD sequence containing all D-amino acids is Ac-RADARADARADARADARGDS-CONH2) shows admirable bioactivity for both cell culture and bone regeneration. Here, we describe the fabrication of a favorable biomaterial material (nHA/PA66/D-RADA16-RGD). METHODS: Proteinase K and circular dichroism spectroscopy were employed to test the stability and secondary structural properties of peptide D-RADA16-RGD respectively. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to characterize the surface of these materials. Confocal laser scanning (CLS), cell counting kit-8 tests (CCK-8), alizarin red S staining, cell immunofluorescence analysis and Western blotting were involved in vitro. Also biosafety and bioactivity of them have been evaluated in vivo. RESULTS: Proteinase K and circular dichroism spectroscopy demonstrated that D-RADA16-RGD in nHA/PA66 was able to form stable-sheet secondary structure. SEM and TEM showed that the D-RADA16-RGD material was 7–33 nm in width and 130–600 nm in length, and the interwoven pore size ranged from 40 to 200 nm. CLS suggests that cells in nHA/PA66/ D-RADA16-RGD group were linked to adjacent cells with more actin filaments. CCK-8 analysis showed that nHA/PA66/ D-RADA16-RGD revealed good biocompatibility. The results of Alizarin-red S staining and Western blotting as well as vivo osteogenesis suggest nHA/PA66/D-RADA16-RGD exhibits better bioactivity. CONCLUSION: This study demonstrates that our nHA/PA66/D-RADA16-RGD composite exhibits reasonable mechanical properties, biocompatibility and bioactivity with promotion of bone formation.

Examining the Day-of-the-Week Effects in Chinese Stock Markets: New Evidence from a Stochastic Dominance Approach

ZHUO QIAO,WEIWEI QIAO,Wing-Keung Wong 연세대학교 동서문제연구원 2011 Global economic review Vol.40 No.3

Many researchers have investigated the existence of day-of-the-week effects in different financial markets. However, they have usually adopted a parametric approach, which is known to have a few limitations. This paper adopts a non-parametric stochastic dominance (SD) approach to examine the day-of-the-week effects in Chinese stock markets. In contrast to the extensive evidence of day-of-the-week effects disclosed by a parametric mean-variance (MV) approach, our SD tests show that the day-of-the-week effect is much weaker. We find that there are only Wednesday effects in Chinese A-share and B-share stock markets.

Knockdown of the M2 Isoform of Pyruvate Kinase (PKM2) with shRNA Enhances the Effect of Docetaxel in Human NSCLC Cell Lines In Vitro

Tiankui Qiao,Tiankui Qiao,Xibing Zhuang,Wei Chen,Na Xing,Qi Zhang 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.6

Purpose: The aim of our study was to explore the relationships between the M2 isoform of pyruvate kinase (PKM2) and the sensitivityof human non-small cell lung cancer (NSCLC) cells to docetaxel in vitro. Materials and Methods: With the method of plasmid transfection, we silenced the expression of PKM2 successfully in A549 and H460 cells. Western blotting and real-time PCR were applied to detect PKM2 expression at protein and gene levels. Cell viability was examined by CCK8 assay. Cell cycle distribution and apoptosis were examined by flow cytometry. P21 and Bax were detected. Results: Expression of PKM2 mRNA and protein were significantly decreased by shRNA targeting PKM2. Silencing of PKM2 increaseddocetaxel sensitivity of human NSCLC A549 and H460 cells in a collaborative manner, resulting in strong suppression of cell viability. The results of flow cytometric assays suggested that knockdown of PKM2 or docetaxel treatment, whether used singlyor in combination, blocked the cells in the G2/M phase, which is in consistent with the effect of the two on the expression of p21. Cells with PKM2 silencing were more likely to be induced into apoptosis by docetaxel although knockdown of PKM2 alone can’t induce apoptosis significantly, which is in consistent with the effect of the two on Bax expression. Conclusion: The results suggest that PKM2 knockdown could serve as a chemosensitizer to docetaxel in non-small lung cancer cells through targeting PKM2, leading to inhibition of cell viability, increase of cell arrest of G2/M phase and apoptosis.

Examining Stock Volatility in the Segmented Chinese Stock Markets: A SWARCH Approach

ZHUO QIAO,WEIWEI QIAO,Wing-Keung Wong 연세대학교 동서문제연구원 2010 Global economic review Vol.39 No.3

This study adopts the SWARCH model to examine the volatile behavior and volatility linkages among the four major segmented Chinese stock indices.We find strong evidence of a regime shift in the volatility of the four markets, and the SWARCH model appears to outperform standard generalized autoregressive conditional heteroskedasticity (GARCH) family models. The evidence suggests that, compared with the A-share markets, B-share markets stay in a high-volatility state longer and are more volatile and shift more frequently between high- and low-volatility states. In addition, the relative magnitude of the high-volatility compared with that of the low-volatility state in the B-share markets is much greater than the case in the two A-share markets. B-share markets are found to be more sensitive to international shocks, while A-share markets seem immune to international spillovers of volatility. Finally, analyses of the volatility spillover effect among the four stock markets indicate that the A-share markets play a dominant role in volatility in Chinese stock markets.

Ceratovacuna atrovirensa Qiao, a new species from China (Aphididae: Hormaphdinae)

Jiang Li-Yun,Chen Jing,Xu Ying,Qiao Ge-xia 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Ceratovacuna atrovirensa Qiao sp.n., a woolly aphid on Indocalamus tessellatus from China, is described and illustrated. The new species is supported by both morphological and molecular data. An updated key to apterous viviparous females of Ceratovacuna is provided. The type specimens are deposited in the National Zoological Museum of China, Institute of Zoology, the Chinese Academy of Sciences, Beijing, China and the Natural History Museum, London, United Kingdom.

Syntrophic Propionate Degradation Response to Temperature Decrease and Microbial Community Shift in an UASB Reactor

( Qiao Ying Ban ),( Jian Zheng Li ),( Li Guo Zhang ),( Ajay Kumar Jha ),( Yu Peng Zhang ),( Bin Ling Ai ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.3

Propionate is an important intermediate product during the methane fermentation of organic matter, and its degradation is crucial for maintaining the performance of an anaerobic digester. In order to understand the effect of temperature on propionate degradation, an upflow anaerobic sludge blanket (UASB) reactor with synthetic wastewater containing propionate as a sole carbon source was introduced. Under the hydraulic retention time (HRT) of 10 h and influent propionate of 2,000 mg/l condition, propionate removal was above 94% at 30-35ºC, whereas propionate conversion was inhibited when temperature was suddenly decreased stepwise from 30ºC to 25ºC, to 20ºC, and then to 18ºC. After a long-term operation, the propionate removal at 25ºC resumed to the value at 30- 35ºC, whereas that at 20ºC and 18ºC was still lower than the value at 35ºC by 8.1% and 20.7%, respectively. Microbial community composition analysis showed that Syntrophobacter and Pelotomaculum were the major propionate-oxidizing bacteria (POB), and most POB had not changed with temperature decrease in the UASB. However, two POB were enriched at 18ºC, indicating they were low temperature tolerant. Methanosaeta and Methanospirillum were the dominant methanogens in this UASB and remained constant during temperature decrease. Although the POB and methanogenic composition hardly changed with temperature decrease, the specific CODPro removal rate of anaerobic sludge (SCRR) was reduced by 21.4%-46.4% compared with the control (35ºC) in this system.

Effect of Different Preharvest and Postharvest Treatments on Glucoraphanin Content and Quinone Reductase Inducer Activity in Broccoli Florets

Qiao-Mei Wang,Chao-Jiong Xu,Jing Yuan,De-Ping Guo 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Estimating functional brain networks by incorporating a modularity prior

Qiao, Lishan,Zhang, Han,Kim, Minjeong,Teng, Shenghua,Zhang, Limei,Shen, Dinggang ACADEMIC PRESS 2016 NEUROIMAGE Vol.141 No.-

<P><B>Abstract</B></P> <P>Functional brain network analysis has become one principled way of revealing informative organization architectures in healthy brains, and providing sensitive biomarkers for diagnosis of neurological disorders. Prior to any post hoc analysis, however, a natural issue is how to construct “ideal” brain networks given, for example, a set of functional magnetic resonance imaging (fMRI) time series associated with different brain regions. Although many methods have been developed, it is currently still an open field to estimate biologically meaningful and statistically robust brain networks due to our limited understanding of the human brain as well as complex noises in the observed data. Motivated by the fact that the brain is organized with <I>modular</I> structures, in this paper, we propose a novel functional brain network modeling scheme by encoding a <I>modularity prior</I> under a <I>matrix-regularized</I> network learning framework, and further formulate it as a sparse low-rank graph learning problem, which can be solved by an efficient optimization algorithm. Then, we apply the learned brain networks to identify patients with mild cognitive impairment (MCI) from normal controls. We achieved 89.01% classification accuracy even with a simple feature selection and classification pipeline, which significantly outperforms the conventional brain network construction methods. Moreover, we further explore brain network features that contributed to MCI identification, and discovered potential biomarkers for personalized diagnosis.</P>

On the Ecological Significances of Confucian Music Theory

( Qiao Qingju ) 성균관대학교 유교문화연구소 2011 儒敎文化硏究(中文版) Vol.0 No.15

在儒家文化中, “樂”幷不完全等同于現代意義的音樂, 而是禮的一種;它不是單純的人文活動, 而是依賴于氣的運行, 服從幷致力于氣的運行的“和”以及天地的“和”的一種貫通天人的活動。這意味着樂是人與自然相溝通的中介, 具有促進天地万物和諧生生的生態作用。儒家樂理論把音樂分爲聲、音、樂。聲指人發出的聲音;音指不同的聲調按照一定的節律結合起來, 類似現代意義的音樂;樂則是禮的一種, 是音樂與舞蹈的結合。儒家文化認爲, 樂的六律、六呂都是順應天地之氣的, 具有本體基礎, 是“天地之命”。樂與万物的道理相貫通, 表現了天地的和諧, 能勾與神明的德性相通, 迎送天地四方的神靈, 促成各種事物的生成。“樂以敦和”。對于前代的樂, 如黃帝的《咸池》等, 儒家也都給予了生態性的解釋。以上均表明了儒家樂文化的生態意義。 In ancient Confucian culture, Yue/music is not exactly the same as modern music, but a kind of rites. It, being not only a sort of human cultural activity, but also, through depending on and being determined by the movement of vital force, functions in conformity with and more importantly is dedicated to the harmonic movement of the vital force, which means that it, with a function of promoting the harmonic living and generation of myriad thing between Heaven and Earth, is a medium of mutual penetration and communication in-between human and nature. In Confucian musical theory, music is divided into 3 parts, namely, voice or sound and tone, music. Sound, also called voice, is that which arises from man’s heart when it is moved by external things. Different sounds being blended together in a way of forming modulations and patterns come out tone, something similar to modern music. Confucian music belongs to rites; it is a performance consisting of singing and playing musical instruments, dancing that sometimes displays shields and axes. By Confucian culture, music, being the mandate of the Heaven and Earth and its 12 bamboo pitch-pipe conforming to the vital force, has an ontological foundation; It, with its principles being connected with those of myriad thing in-between Heaven and Earth and, its function expressing the harmony of Heaven and Earth, is able to communicate with the virtue of those spiritual beings in-between Heaven and Earth and, to welcome and send off them, so as to enhance the living and harvest of myriad things. “The function of music is to promote harmony.” As for those former sage-kings’ music such as the Emperor Huang’s Xianchi, Confucian thinkers gave them ecological explanations. Above shows the ecological significances of Confucian musical culture. [Article in Chinese]

Preparation and spectral characteristics of Ce3+-activated boroaluminate LaAl2B3O9

Qiao, X.,Cheng, Y.,Qin, C.,Tao, Z.,Huang, Y.,Cai, P.,Chen, C.,Seo, H. J. Springer Science + Business Media 2015 APPLIED PHYSICS A MATERIALS SCIENCE AND PROCESSING Vol.118 No.2