Characterization of a novel variant HMW‐glutenin gene from Elymus canadensis

Qian‐Tao Jiang,Yu‐Ming Wei,Tao Liu,Ji‐Rui Wang,Zhi‐En Pu,Xiu‐Jin Lan,You‐Liang Zheng,Zhen‐Xiang Lu 한국유전학회 2010 Genes & Genomics Vol.32 No.4

High molecular weight (HMW) glutenin subunits (GS) play a key role in the determination of end‐use quality of wheat and other cereal crops. In this study, we report the isolation and characterization of both promoter region and ORF of novel HMW‐GS allele 1St1.3 from a perennial Triticeae species,Elymus canadensis. The amino acid (AA) sequences of E. canadensis 1St1.3 were deduced as 434 aa. Its protein primary structure comprises a signal peptide with a conserved N‐terminal domain, a central repetitive domain and a C‐terminal domain. E. canadensis 1St 1.3 possesses several distinct characteristics which are different from those of wheat HMW‐GSs. The N‐terminal domains of E. canadensis 1St 1.3 resemble that of y‐type subunits, while their C‐terminal domains are more similar to x‐type subunits. The deletion of 85 bp fragment has been observed in promoter region of 1St 1.3, however which has not interrupted the expression of this gene. Our results indicate that 1St 1.3 is novel HMW‐GS variants which will be valuable for enhancing our understanding of structural differentiation and the evolutionary relationship among HMW‐GSs in Triticeae species.

Hypoxia Mediates Runt-Related Transcription Factor 2 Expression via Induction of Vascular Endothelial Growth Factor in Periodontal Ligament Stem Cells

Manjing Deng,Qian Xu,Zhihua Liu1,Ling Guo,Rui Liu,Rulei Li,Xiang Chu,Jiajia Yang,Jia Luo,Faming Chen 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.11

Periodontitis is characterized by the loss of periodontal tissues, especially alveolar bone. Common therapies cannot satisfactorily recover lost alveolar bone. Periodontal ligament stem cells (PDLSCs) possess the capacity of self-renewal and multilineage differentiation and are likely to recover lost alveolar bone. In addition, periodontitis is accompanied by hypoxia, and hypoxia-inducible factor-1α (HIF-1α) is a master transcription factor in the response to hypoxia. Thus, we aimed to ascertain how hypoxia affects runt-related transcription factor 2 (RUNX2), a key osteogenic marker, in the osteogenesis of PDLSCs. In this study, we found that hypoxia enhanced the protein expression of HIF-1α, vascular endothelial growth factor (VEGF), and RUNX2 ex vivo and in situ. VEGF is a target gene of HIF-1α, and the increased expression of VEGF and RUNX2 proteins was enhanced by cobalt chloride (CoCl2, 100 μmol/L), an agonist of HIF-1α, and suppressed by 3-(5′-hydroxymethyl-2′-furyl)-1-benzyl indazole (YC-1, 10 μmol/L), an antagonist of HIF-1α. In addition, VEGF could regulate the expression of RUNX2, as RUNX2 expression was enhanced by human VEGF (hVEGF165) and suppressed by VEGF siRNA. In addition, knocking down VEGF could decrease the expression of osteogenesis-related genes, i.e., RUNX2, alkaline phosphatase (ALP), and type I collagen (COL1), and hypoxia could enhance the expression of ALP, COL1, and osteocalcin (OCN) in the early stage of osteogenesis of PDLSCs. Taken together, our results showed that hypoxia could mediate the expression of RUNX2 in PDLSCs via HIF-1α-induced VEGF and play a positive role in the early stage of osteogenesis of PDLSCs

[BOOK REVIEW] China’s leftover women: Late marriage among professional women and its consequences

Qian LIU 이화여자대학교 아시아여성학센터 2015 Asian Journal of Women's Studies(AJWS) Vol.21 No.4

The XPD Lys751Gln Polymorphism has Predictive Value in Colorectal Cancer Patients Receiving Oxaliplatin-Based Chemotherapy: a Systemic Review and Meta-analysis

Qian, Ying-Ying,Liu, Xin-You,Pei, Dong,Xu, Jia-Li,Shen, Hua,Chen, Xiao-Feng,Liu, Yi-Qian,Shen, Li-Zong,Shu, Yong-Qian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: The predictive value of the xeroderma pigmentosum group D (XPD) Lys751Gln polymorphism regarding clinical outcomes of patients with colorectal cancer (CRC) receiving oxaliplatin-based chemotherapy has been evaluated in numerous published studies, but the results remain inconclusive. Therefore, we performed a meta-analysis to determine the precise role of the XPD Lys751Gln polymorphism in this clinical situation and optimize individual chemotherapy. Materials and Methods: A multiple search strategy was used to identify eligible studies. Pooled odds ratios (ORs), generalized odds ratio (ORG) and their 95% confidence intervals (CIs) were used to estimate the objective response, while hazard ratios (HRs) with 95%CIs were used for progression-free survival (PFS) and overall survival (OS). Results: A total of 17 studies including 2,286 patients met the inclusion criteria. Overall, the XPD 751Gln allele was associated with a non-significant reduced objective response to oxaliplatin-based chemotherapy in all patients or in the Asian and Caucasian subgroups. However, poor PFS and OS of CRC patients treated with oxaliplatin-based regimens were significantly related to the XPD 751Gln allele in the dominant model (PFS: HR=2.10, 95%CI: 1.65-2.67; OS: HR=3.18, 95%CI: 1.57-6.47). On stratified analysis by ethnicity, these relationships were more pronounced in Asians (PFS: HR=2.49, 95%CI: 1.79-3.47; OS: HR=5.25, 95%CI: 3.46-7.94) than in Caucasians (PFS: HR=1.73, 95%CI: 1.22-2.46; OS: HR=1.78, 95%CI: 1.06-2.99). Conclusions: The XPD Lys751Gln polymorphism may have prognostic value in patients with CRC undergoing oxaliplatin-based chemotherapy.

A Two-Step Screening Algorithm to Solve Linear Error Equations for Blind Identification of Block Codes Based on Binary Galois Field

( Qian Liu ),( Hao Zhang ),( Peidong Yu ),( Gang Wang ),( Zhaoyang Qiu ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.9

Existing methods for blind identification of linear block codes without a candidate set are mainly built on the Gauss elimination process. However, the fault tolerance will fall short when the intercepted bit error rate (BER) is too high. To address this issue, we apply the reverse algebra approach and propose a novel “two-step-screening” algorithm by solving the linear error equations on the binary Galois field, or GF(2). In the first step, a recursive matrix partition is implemented to solve the system linear error equations where the coefficient matrix is constructed by the full codewords which come from the intercepted noisy bitstream. This process is repeated to derive all those possible parity-checks. In the second step, a check matrix constructed by the intercepted codewords is applied to find the correct parity-checks out of all possible parity-checks solutions. This novel “two-step-screening” algorithm can be used in different codes like Hamming codes, BCH codes, LDPC codes, and quasi-cyclic LDPC codes. The simulation results have shown that it can highly improve the fault tolerance ability compared to the existing Gauss elimination process-based algorithms.

A New Lupane-Triterpene Glycoside from the Leaves of Acanthopanax gracilistylus

Liu, Xiang-Qian,Chang, Seung-Yeup,Park, Sang-Yong,Nohara, Toshihiro,Yook, Chang-Soo The Pharmaceutical Society of Korea 2002 Archives of Pharmacal Research Vol.25 No.6

A new and two known lupane-triterpene glycosides were isolated from the hot MeOH fraction of the leaves of Acanthopanax gracilistylus W. W. Smith. Based on the physical properties and spectroscopic data, their chemical structures were determined as acankoreoside A (1), acankoreoside D (2), and $3{\alpha}-hydroxy-lup-23-al-20(29)-en-28-oic$ acid $28-O-{\alpha}-L-rhamnopyranosyl-(1{\rightarrow}4)-{$beta}-D-glucopyranosyl-(1{\rightarrow}6)-{\beta}-D-glucopyranosyl$ ester (3), respectively. To our best knowledge, compand 3 appears to be novel, which was named as wujiapioside A.

Pyrolysis of wood species based on the compositional analysis

Qian Liu,Kaige Wang,Zhongyang Luo,Kefa Cen,Shurong Wang 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.2

Based on the Van Soest method, the components in Chinese fir and fast-growing poplar were quantified, and the fiber present was separated into three fractions: neutral detergent fiber, acid detergent fiber and strong acid detergent fiber. Microstructure of the fibers was investigated by a Fourier transform infrared spectrometry. Cellulose and hemicellulose both represent the characteristics of polysaccharides, while lignin has dissimilar structure. Pyrolysis of fir, poplar and the detergent fibers was carried out on a thermogravimetric analyzer coupled with FTIR spectrometry. After the removal of extractives and soluble minerals, pyrolysis of NDF shows the haracteristics of the three main components. Hydrocarbons, aldehydes, ketones, acids, alcohols and others are generated due to the primary pyrolysis of hemicellulose and cellulose in single stages. Phenols and alcohols are the dominant volatiles released from pyrolysis of lignin in two successive stages, respectively.

Highly Efficient Shoot Regeneration from Cotyledonary Nodes of Vegetable Soybean

Qian-Qian Liu,Gang Chen,Jun-Yi Gai,Yue-Lin Zhu,Li-Fei Yang,Guo-Ping Wei,Cong Wang 한국원예학회 2010 원예과학기술지 Vol.28 No.2

풋콩(Glycine max (L.) Merrill) 6개 품종의 자엽절 절편체로부터 thidiazuron(TDZ)과 NAA 농도를 달리한 배지에서 신초의 효율적인 재분화를 조사한 후, 가장 효과가 좋은 생장조절물질 조합으로 배지 종류, 접종방법 및 품종별 신초 재분화율을 조사하였다. 또한 절편체를 배지에 수직 또는 수평으로 치상하는 방법과 B5, 1/2 B5, MS, 1/2 MS, MSB(MS salts + B5 organics) 등의 5가지 기본 배지에 관하여 시험하였다. B5 배지에 1㎎?L<SUP>-1</SUP> TDZ, 0.05㎎?L<SUP>-1</SUP> NAA, 5㎎?L<SUP>-1</SUP> AgNO₃를 첨가한 처리에서 6개 품종의 신초 재분화율이 55.3-88.9%로 높았다. ‘L?ling No. 1’의 경우 수직으로 치상하는 것이 수평으로 치상하는 것보다 신초 재분화율이 37.5% 더 높았다. 따라서 풋콩은 TDZ를 처리한 B5 배지에 자엽절을 수직으로 치상할 때 신초 재분화율이 높았다. To establish a highly efficient system for shoot regeneration in vegetable soybean (Glycine max (L.) Merrill), explants were obtained from six genotypes and adventitious shoots were regenerated from cotyledonary nodes cultured on medium supplemented with different concentrations of N-phenyl-N′-1, 2, 3-thiadiazol-5-ylurea (thidiazuron TDZ) and 1-naphthylacetic acid (NAA). The most effective combination of plant growth regulators was selected first and then the effects of medium types, inoculation methods and genotypic differences on shoot regeneration were studied. The explants were inserted either vertically or horizontally into the medium and five basal media, including B5, ½ B5, MS, ½ MS, and MSB (MS salts and B5 organics), were tested. The shoot regeneration frequency of the six genotypes ranged from 53.5% to 88.9% and three of them reached 88.9%, 87.5% and 83.3%, respectively, on B5 medium supplemented with 1 ㎎?L<SUP>-1</SUP> TDZ, 0.05 ㎎?L<SUP>-1</SUP> NAA, and 5 ㎎?L<SUP>-1</SUP> AgNO₃. The shoot regeneration frequency of explants cultured on B5 medium was significantly higher than that of the other four basal media. The vertically inserted explants were found to yield a higher shoot regeneration frequency than that of horizontally inserted ones; the maximum difference of regenerating percentages between the two methods was 37.5%. In summary, TDZ was an efficient plant growth regulator for shoot induction. B5 medium and vertically inserted explants promoted shoot regeneration. We believe this highly efficient shoot regeneration system will provide foundation for the further transgenic studies in vegetable soybean.

High Efficient in Vitro Shoot Regeneration from Cotyledonary Nodes of Vegetable Soybean

Qian-Qian Liu,Jun-Yi Gai,Yue-Lin Zhu,Gang Chen,Li-Fei Yang,Guo-Ping Wei,Cong Wang 한국원예학회 2008 한국원예학회 기타간행물 Vol.- No.-

Simultaneous adsorption of phenol and Cu2+ from aqueous solution by activated carbon/chitosan composit

Qian Liu,Bingchao Yang,Lujie Zhang,Ruihua Huang 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.9

A multifunction adsorbent was synthesized by incorporating AC into CTS, and the ratio of AC to CTSwas 1/1. The resultant was called activated carbon (AC)/chitosan (CTS) composite. The simultaneous adsorption ofphenol and Cu2+from aqueous solution onto AC/CTS composite was investigated by a batch procedure. The adsorptionprocesses for both Cu2+and phenol obeyed the pseudo second-order kinetic model. Phenol was prone to be adsorbedmore quickly as compared with Cu2+when they coexisted in solution. The adsorption behavior of both phenol and Cu2+followed the Langmuir isotherm. The maximum adsorption capacities of phenol and Cu2+were 34.19 mg/g and 74.35mg/g at 293 K, respectively. No obvious competitive adsorption existed between phenol and Cu2+.