Leptin protects rat articular chondrocytes from cytotoxicity induced by TNF-α in the presence of cyclohexamide

Lee, S.W.,Rho, J.H.,Lee, S.Y.,Kim, J.H.,Cheong, J.H.,Kim, H.Y.,Jeong, N.Y.,Chung, W.T.,Yoo, Y.H. Published for the Society by Baillère Tinda 2015 OSTEOARTHRITIS AND CARTILAGE Vol.23 No.12

Objective: Although leptin appears to be an important local and systemic factor influencing cartilage homeostasis, the role of leptin in chondrocyte death is largely unknown. Tumor necrosis factor α (TNF-α) is a pro-inflammatory cytokine that plays a central role in the pathogenesis of articular diseases. This study examines whether leptin modulates TNF-α-induced articular chondrocyte death. Methods: Primary rat articular chondrocytes were isolated from knee joint cartilage slices. To induce cell death, the chondrocytes were treated with TNF-α. To examine whether leptin modulates the extent of TNF-α-mediated chondrocyte death, the cells were pretreated with leptin for 3 h before TNF-α treatment followed by viability analysis. To examine the mechanism by which leptin modulates the extent of TNF-α-mediated chondrocyte death, we utilized mitochondrial membrane potential (MMP) measurements, flow cytometry, nuclear morphology observation, co-immunoprecipitation, western blot analysis and confocal microscopy. Results: We demonstrated that leptin suppresses TNF-α induced chondrocyte death. We further found that apoptosis partially contributes to TNF-α induced chondrocyte death while necroptosis primarily contributes to TNF-α induced chondrocyte death. In addition, we observed that leptin exerts anti-TNF-α toxicity via c-jun N-terminal kinase (JNK) in rat articular chondrocytes. Conclusion: Based on our findings, we suggest that the leptin present in the articular joint fluid protects articular chondrocytes against cumulative mechanical load and detrimental stresses throughout a lifetime, delaying the onset of degenerative changes in chondrocytes. We can further hypothesize that leptin protects articular chondrocytes against destructive stimuli even in the joints of osteoarthritis (OA) patients.

물리 화학적 방법에 의한 우모분증의 케라틴태 단백질 추출 및 아미노산 함량에 미치는 영향

이남형(N . H . Lee),김영붕(Y . B . Kim),김희주(H . J . Kim),성기승(K . S . Seong),노정해(J . H . Rho),한찬규(C . K . Han) 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.1

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발효처리된 우모분이 쥐의 생체내 아미노산 이용물에 미치는 영향

이남형(N . H . Lee),김영붕(Y . B . Kim),김희주(H . J . Kim),성기승(K . S . Seong),노정해(J . H . Rho),한찬규(C . K . Han),안철(C . Ahn) 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.1

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지방산 결합단백질에 관한 연구 동향

권영아,노숙령,김혜경,함영태 中央大學校 食糧資源硏究所 1996 食糧資源硏究所 論文集 Vol.8 No.1

ABSTRACT Long chain free fatty acid (FFA) are the main energy source for many mammalian cell, and FFA metabolism is dependent upon the flux of FFA to sites of intra-cellular processing. Fatty Acid Binding Proteins (FABPs) are a family of abundant cytosolic proteins of low M.W.14-15 kDa. Although their precise physilogical role remains hypothetical, it has been proposed as a FFA transfer protein and as a binding protein responsible for controlling intracellular FFA concentration. Furthermore, significant correlations have been observed between the tissue content of FABP and the rate of FFA uptake or utilization in several different manipulations such as diet and hypolipidemic drugs. However, to date there has been no report which examines the role of FABP in physiological manipulations such as fatty liver, diabetes, obesity, hypercholesterolemia. Since lipid metabolism is abnormal and the liver is greatly affected in these disease, it is possible that FABP may be abnormal in these livers. Therefore, the realtionships of liver FABP and the disease with lipid abnormalities, especially fatty liver induced by alcohol and high cholesterol diet are examined in our lab. Where FABP is believed to be involved in FFA binding and transfer, cellular FABP amount as well as its binding activity are affected. If we further find the way to increase the expression of FABP in liver, it could be one means of settle for these kinds of diseases from lipid abnormality.

돼지고기를 단백질 급원으로 한 식이가 쥐의 체내 카드뮴 대사에 미치는 영향에 대한 연구

정용기,노정해,이남형,한찬규 한국축산학회 1997 한국축산학회지 Vol.39 No.5

This study was performed to investigate the effect of feeding pork in the place of casein on cadmium toxicity in rats. Thirty six male rats of Sprague-Dawley weighing 225.2?9.3g were randomly blocked into six groups according to body weights. Three levels of cadmium in drinking water(0, 25 and 50 ppm) had been fed for 5 weeks. Pork replaced dietary casein as a protein source. Weight gains in either cadmium-added or pork-fed group tended to be lower than those of the control group(Cd 0 ppm and casein). The cadmium addition and pork feedings affect significantly feed efficiency according to factor analysis results. Hematocrit was decreased with cadmium intake(p$lt;0.05). Cadmium accumulation in liver showed a tendency to diminish in the pork-fed group among the cadmium-exposed groups. Hepatic enzyme activity of glutatione peroxidase and superoxide dismutase showed no significant differences among the treatments. Urinary and fecal cadmium excretions increased with pork feeding. The results indicate that pork feeding as protein source in diet may alleviate the cadmium toxicity in rats.

산란계의 연령과 면역주기에 따른 난황 중의 Streptococcus mutans 특이 항체 함량

성기승,김영붕,노정해,이남형,한찬규,손동화 한국축산학회 1999 한국축산학회지 Vol.41 No.5

Streptococcus mutans-specific IgY content change, laying rate, egg weight and weight change were measured for 17-week and 30-week old hens. Vaccinations with Streptococcus mutans were made two times(eight week interval), three times(four week interval) and five times(two week interval), respectively. It was observed that the laying rate of vaccinated hens was likely lower than that of non-vaccinated group. No effect on body weight by vaccination was found out. Egg weight did not show a certain tendency by vaccination. Anti-S. mutans IgY started to be detected two weeks after the lst vaccination for 30-week old hens. It was not detected for non-vaccinated group. The antibody activity was consistently detected after 8 weeks from the last vaccination. The measurement of total IgY and S. mutans-specific IgY in the egges from vaccinated hens revealed that IgY tended to increase with the number of vaccination. S. mutans-specific IgY content of five-time vaccinated l7-week hens was much higher than that of 30-week old hens. To obtain steady amount of specific IgY, multiple vaccination with two week interval was recommended.

생약 복합제제의 약효 연구(제16보) : 팔미환의 신성 고혈압 효과에 관한 연구

노영수(Y. S. Rho),홍남두(N. D. Hong),김수옥(S. O. Kim),김남재(N. J. Kim) 한국생약학회 1984 생약학회지 Vol.15 No.1

팔미환은 중경전서에 처음으로 수록된 이래 금괴요락, 동의보감, 방약합편 등에 기록되어 주로 구갈이나 배뇨 이상등에 활용되고 있는 처방이다. 본 방제는 건지황, 산수유, 산약, 택사, 복령목단피, 계지 및 부자로 구성되어 있으며 이미 건지황, 산수유, 산약, 계지등은 자양강장효과를 건지황, 택사, 산약, 산수유등은 지갈효과를 택사, 복령, 부자등은 이뇨효과를 갖고 있다고 보고되었다. 따라서 팔미환은 구갈이나 배뇨 이상을 지표로 하는 당뇨병과 부종, 뇨량의 감소 혹은 증대등의 신기능 이상등에 작용하는 생약들로 구성되어 있으며, 그 효능을 현재의 관점에서 보면 당뇨병에 상당하는 증상과 여러 종류의 신장질환을 수반하는 부종, 배뇨곤란, 신성 고혈압 등에 유효한 것으로 생각되어 진다. 연자등은 팔미환의 한방문헌적 효능과 임상에 응용되고 있는 효과를 약리학적 측면에서 실혈적으로 구명하고자, 우선 정상 흰쥐와 가토에 대한 이뇨효과와 HgCl₂투여로 급성신부전을 유발시킨 흰쥐의 병리 model에 대한 이뇨효과, 그리고 가토의 급성신부전 병태 model 대한 혈압강하작용을 검토한바 다음과 같은 성적을 얻었다. 1. 정상 흰쥐와 가토에 대하여 검액 투여로 뇨량 Na^+및 Cl^- 이유의 하계증가됨을 알 수 있었다. 2. 급성신부전 흰쥐에 대하여 검액 투여로 뇨량, Na^+ 및 Cl^- 이유의 하게 증가됨을 알 수 있었다. 3, 정상 및 급성신부전 흰쥐에 대하여 검액투여로 혈장중의 urea nitrogen 함량이 유의성 있게 증가됨을 알 수 있었다. 4. 정상 가토 및 고양이에 대하여는 혈압에는 영향을 주지 않으나 급성신부전 가토의 병태 model에 대해서는 용량 의존적으로 혈압강하작용이 인정되었다.

인중백이 진통, 진정, 혈압 강하작용에 미치는 영향

노영수(Y. S. Rho),홍남두(N. D. Hong),조영환(Y. H. Cho) 한국생약학회 1984 생약학회지 Vol.15 No.1

인중백은 뇨호의 내벽이나 저면에 자연 침전되어 얻어진 물질로서 탕화작창강화 심격열 제출혈등에 사용한다고 하며, 추석은 추기에 동자뇨를 원료로 하여 제조한 것으로서 제법으로는 양연번 음연법 음양연법등이 있고, 청심 강하지제로 심장병 고혈압 및 중풍치료 제출혈에 활용된다고 되어 있으나, 현재 임상에서는 연중백과 추석을 동일하게 취급하고 혼합 거래되고 있는 실정이다. 이에 저자등은 민중백과 추석이 그 기본 원료가 인뇨인 것은 동일하나 효능이 다르게 기재되어 있고 그 구분이 명확치 못한 것에 흥미를 갖고 이에 대한 차이를 구명하기 위한 연구의 일환으로 인중백에 대한 효능을 실험동물을 통하여 실험한 결과 다음과 같은 성적을 얻었다. 1. 중추 신경의 진통작용과 진정작용이 인정되었다. 2. 생쥐, 가토의 적풀회장관에 대하여 강한 이완작용을 나타내었고, Acetylcholine과 BaCl₂의 수축에 강한 길항작용을 나타내어 장 평활근의 근원성 이완작용이 인정되었다. 3. 혈관 평황근 이완에 의한 혈관작용과 혈압 강하작용이 인정되었다. 이상과 같은 실험결과로 보아 인중백은 진통작용, 혈관확장작용 및 혈압강하작용이 있는 것을 알 수 있었다.

Hepatic STAMP2 alleviates high fat diet-induced hepatic steatosis and insulin resistance

Kim, H.Y.,Park, S.Y.,Lee, M.H.,Rho, J.H.,Oh, Y.J.,Jung, H.U.,Yoo, S.H.,Jeong, N.Y.,Lee, H.J.,Suh, S.,Seo, S.Y.,Cheong, J.,Jeong, J.S.,Yoo, Y.H. Elsevier Science Publishers 2015 Journal of hepatology Vol.63 No.2

Background & Aims: Most studies on the role of STAMP2 in metabolism have used adipose tissue. Little knowledge exists concerning the role of STAMP2 in the liver, which is a metabolically central target. We hypothesized that STAMP2 is involved in non-alcoholic fatty liver disease (NAFLD) pathogenesis. Methods: We examined our hypothesis using human NAFLD patient pathology samples and a high-fat diet (HFD)-induced NAFLD mouse model. The molecular mechanism underlying hepatic STAMP2-mediated lipid imbalance was explored using an oleic acid (OA)-induced NAFLD in vitro model. Results: Noticeably, the expression level of STAMP2 protein was reduced in the livers obtained from NAFLD patients and HFD-induced NAFLD mice. In vivo knockdown of hepatic STAMP2 by siRNA accelerated hepatic steatosis and insulin resistance in mice fed a HFD. Conversely, the delivery of adenoviral STAMP2 (Ad-STAMP2) improved hepatic steatosis in HFD-induced NAFLD mice. The expression of lipogenic or adipogenic factors was increased in both in vitro and in vivo NAFLD models but was reversed by Ad-STAMP2. Adenoviral overexpression of STAMP2 improved insulin resistance in the HFD-induced NAFLD mice. In vivo and in vitro assays demonstrated that STAMP2 modulates insulin sensitivity and glucose metabolism and that STAMP2 counteracts OA-induced insulin resistance by modulating insulin receptor substrate-1 stability. Conclusions: The present study revealed that hepatic STAMP2 plays a pivotal role in preventing HFD-induced NAFLD and that STAMP2 overexpression improves hepatic steatosis and insulin resistance in NAFLD. Our findings indicate that STAMP2 may represent a suitable target for interventions targeting NAFLD.

진공포장된 수입 냉장돈육의 저장성에 관한 연구

김영붕(Y . B . Kim),노정해(J . H . Rho),이남형(N . H . Lee) 한국축산학회 1996 한국축산학회지 Vol.38 No.6

To establish on the shelf-life of pork, vacuum-packaged imported chilled pork(Butt and Ham) were purchased from 2 american compaines(M. Co. and I. Co.). The samples were transported by air and stored at 4±1℃ and 0±1℃, respectively. The chemical composition, total microbes, anaerobic microbes, coliform colony count, volatile basic nitrogen(VBN) and sensory evaluation were determined. In the case of vacuum-packaged chilled pork, the date reaching to 10^6 of total microbes at 4±1℃ was 34 days after slaughtering, for pork from I. Co. and 37 days for M. Co.. At 0±1℃, however, it was 41 days for I. Co. and 48 days for M. Co. Under the storage at 4±1℃, VBN of M. Co. pork reached to 26.0±3.8㎎ % after 41 days and I. Co. pork reached to 19.6±1.4 ㎎% after 34 days, while that of M. Co. after 41 days over 20.0 ㎎% at 0±1℃. The sensory scores of freshness and odor were less than 4, for pork from both I. Co. and stored at 4±1℃ for 37 days and M. Co. stored for 41 days. Those scores of I. Co. pork stored for 41 days and M. Co. pork stored for 55 days under 0±1℃ were also less than 4. Therefore, the shelf life of vacuum-packaged imported chilled pork from I. Co. was 31 days and M. Co. was 38 days after slaughtering at 4±1℃. While that of pork from I. Co. was 38 days after slaughtering and M. Co. was SS days, at 0±1℃.