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Karyotype Analysis and Tandem Repeats in Heterochromatin of Brassica rapa
Ki-Byung Lim(임기병),Tae-Jin Yang(양태진),Jee-Young Park(박지영),Soo-Jin Kwon(권수진),JungSun Kim(김정선),Myung-Ho Lim(임명호),JinA Kim(김진아),Mina Jin(진민아),Yong-Moon Jin(진용문),Seog Hyung Kim(김석형),YP Lim(임용표),JW Bang(방재욱) 한국원예학회 2005 한국원예학회 학술발표요지 Vol.2005 No.5
정향 , 마황 , 계피의 간염 B 형 바이러스 증식 억제 효과
강석연(Seog Youn Kang),김태균(Tae Gyun Kim),박민수(Min Su Park),한형미(Hyung Mee Han),정기경(Ki Kyung Jung),강주혜(Ju Hye Kang),문애리(A Ree Moon),김승희(Seung Hee Kim) 한국응용약물학회 1999 Biomolecules & Therapeutics(구 응용약물학회지) Vol.7 No.2
This study was undertaken to test for anti-Hepatitis B virus (HBV) activity of the aqueous extracts prepared from Eugenic caryophyllate, Ephedra sinica, Cinnamomum cassia. Aqueous extracts were assayed for the inhibition of HBV replication by measurement of HBV DNA and surface antigen (HBsAg) levels in the extracellular medium of HepG2 2.2.15 cells. All extracts decreased the levels of extracellular HBV virion DNA at concentrations ranging from 128 to 256 ㎍/㎖ and inhibited the production of HBsAg dose-dependently. Our findings suggest that these three hebal medicinal plants may have potential to develop as specific anti-HBV drugs in the future.
김석준,이상문,이중기,백효종,손경락,서화령,남근하,문세광 대한소화기학회 1998 대한소화기학회지 Vol.31 No.2
Autoimmune hepatitis is a chronic necroinflammatory liver disorder of unknown cause associated with circulating autoantibodies and a high serum globulin level. This disease occurs predominantly in young wornen, but rarely in postmenopausal woman. A variety of immunosuppressive agents have been used for the management. However, corticosteroid treatment in postmenopausal woman have a higher cummulative frequency of long-term complications and a lower net henefit-risk ratio than in premenopausal woman. A 69-year-old female was diagnosed to have autoimmune hepatitis in our hospital. We report this case with literature review.
DHEA의 항비만 효능 및 ob 유전자 (leptin)의 발현에 미치는 영향
정기경,신미희,한형미,강석연,김태균,강주혜,문애리,김승희 德成女子大學校 藥學硏究所 2000 藥學論文誌 Vol.11 No.1
Leptin, the product of the ob gene, is a small peptide molecule synthesized by white adipocytes with an important role in the regulation of body fat and food intake. Baesd on the evidence that synthesis of leptin is regulated by female sex hormone, estrogen, this present study was investigated whether sex hormone precursor, DHEA, can regulate obese gene expression in lean and genetically obese (ob/ob) mice. Antiobesity activity of DHEA was evaluated by determining body weight, food consumption, epididymal fat weight and serum levels of cholesterol and triglyceride in ICR, C57BL/6J, and ob/ob mice. The treatment of C57BL/6J lean and obese mice with a diet containing 0.3% and 0.6% DHEA resulted in lowered rates of weight gain in comparison to non-treated mice, although much greater response was found in the obese mice. All other concentrations of DHEA (0.015%, 0.06%, 0.15%, 0.3%) except the highest one(0.6%) showed no significant effects on weight gain in ICR mice. Food consumption was significantly decreased in all mice treated with 0.6% DHEA, whereas it was not decreased in ICR mice at lower concentrations than 0.6% DHEA. DHEA decreased significantly epididymal adipose tissue weight and serum triglyceride levels dose dependently in lean and obese mice. However, serum cholesterol levels were decreased at lower concentrations than 0.15% DHEA and increased at concentrations of 0.3% and 0.6% DHEA in lean and obese mice. These increases in serum cholestrol levels at high concentrations of DHEA might result from the fact that DHEA has a cholesterol moiety, thereby interfered the assay system. As an approach to elucidate the mechanism for antiobesity activity of DHEA, we examined mRNA levels of obese gene in the adipocyte and obese gene product (leptin) in the serum. The results showed that DHEA did not affect obese gene expression in ICR and C57BL/6J mice. Therefore, we concluded that antiobesity activity of DHEA was not modulated by obese gene expression.
Characterization of rDNAs and tandem repeats in the heterochromatin of Brassica rapa.
Lim, Ki-Byung,de Jong, Hans,Yang, Tae-Jin,Park, Jee-Young,Kwon, Soo-Jin,Kim, Jung Sun,Lim, Myung-Ho,Kim, Jin A,Jin, Mina,Jin, Yong-Moon,Kim, Seog Hyung,Lim, Yong Pyo,Bang, Jae-Wook,Kim, Ho-Il,Park, Be Korean Society for Molecular Biology 2005 Molecules and cells Vol.19 No.3
<P>We describe the morphology and molecular organization of heterochromatin domains in the interphase nuclei, and mitotic and meiotic chromosomes, of Brassica rapa, using DAPI staining and fluorescence in situ hybridization (FISH) of rDNA and pericentromere tandem repeats. We have developed a simple method to distinguish the centromeric regions of mitotic metaphase chromosomes by prolonged irradiation with UV light at the DAPI excitation wavelength. Application of this bleached DAPI band (BDB) karyotyping method to the 45S and 5S rDNAs and 176 bp centromere satellite repeats distinguished the 10 B. rapa chromosomes. We further characterized the centromeric repeat sequences in BAC end sequences. These fell into two classes, CentBr1 and CentBr2, occupying the centromeres of eight and two chromosomes, respectively. The centromere satellites encompassed about 30% of the total chromosomes, particularly in the core centromere blocks of all the chromosomes. Interestingly, centromere length was inversely correlated with chromosome length. The morphology and molecular organization of heterochromatin domains in interphase nuclei, and in mitotic and meiotic chromosomes, were further characterized by DAPI staining and FISH of rDNA and CentBr. The DAPI fluorescence of interphase nuclei revealed ten to twenty conspicuous chromocenters, each composed of the heterochromatin of up to four chromosomes and/or nucleolar organizing regions.</P>