국내에서 분리된 canine parvovirus의 구조유전자 cloning과 염기서열 분석

박종현,송재영,이중복,현방훈,안수환,전무형 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

In this study gene encoding structural proteins of a CPV isolate was cloned and saquenced to elucidate the molecular genetical properties of the canine parvoviruses isolated from the field. Six recombinant plasmids of pEP3, p1471, p2070, pEP069, pEP338 and p14711p were constructed from the map positions 22 to 98 of RF DNA to clone the VP1 and VP2 genes of CPV-V20. Sequentialy the gene comprising 3780 nucleotides were sequenced by dideoxy chain termination method. When nucleotide sequence of gene encoding the structural proteins of CPV-V20 was compared with those of other strains, CPV-N, CPV-d and CPV-780929 published previously. DNA homologies to CPV-V20 were 99.87% with CPV-NM, 99.73% with CPV-d, 96.85% with CPV-780929 AND 98.4% with FPLV-Carl, respectively. The DNA sequence data of CPV-V20 showed seven point mutations and also deletion of 135 nucleotides from the nucleotide position 4745 to 4879 located in the 3-noncoding region of CPV-N.

신체활동에 따른 요통 예방과 치료에 관한 자세의 생활지도 및 운동요법 연구 고찰

최무현,정대철 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.3

The spine is a pillar of human body. The lumber connects upper body and lower body, protects spinal marrow, and sustain body weight. The relative posture of the discs changes according to the spine's movement and body's postural transformation, The pain, pressure, and temperature are felt through the spinal nerves, When a certain posture or a movement is maintained for a long time, the spinal nerves are extended too much and pain occurs, So, the normal right arrangement of the discs cannot be maintained, A certain movement is sometimes restricted partly or wholly. when the movement is tried, severe pain is felt, To recover normal flexibility of the spines, we should do proper exercise. The general reason of low back pain is the failure of maintaining proper spinal curve. When you stand, sit clown, or lie in a tensed posture or in a bad posture too long, you are prone to have low back pain When your working position requires you to bend your spine for a long time, the same problem occurs, The furniture of which design ignores human body engineering consideration also causes low back pain. Everyday life posture guidance includes the posture guidance of human dynamics, the posture instruction on the bed, using medical gymnastics, and the postural instruction at the recreational events as a sport activity. To prevent low back pain, the normal right spinal curve should be maintained according to each posture and the relative muscles of the lumber should be trained, Spinal muscles, abdominal muscles, and back muscles should be strengthened all together to decentralize body weight that is usually loaded onto the spine, The burden on the spine should be distributed to the waist or abdominal muscles, The muscle training is a very important and effective method to prevent low back pain.

Baculovirus Vector System에 의해 발현된 재조합 Pseudorabies Virus Major Capsid Protein의 면역원성

전무형,안동준,장경수,조용성,박종현,송재영,현방훈,안수환 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

The recombinant pseudorabies virus major capsid protein (rMCP) was produced by expression of the MCP gene in Sf-9 cell using baculovirus transfer vector system. Following evaluation of the immunochemical properties of the rMCP, the immunogenicity of the recombinant subunit protiens were investigated in guinea pig and swine to obtain the preliminary guide line for the subunit vaccine using rMCP and gP50. It was proved that ultrasonication and 30% ammonium sulfate was most efficient to concentrate and purify the protein. The rMCP was safe in mice, guinea pigs and piglets. In guinea pigs, rMCP mixed with various adjuvants induced substantial degree of serum neutralizing antibody titers, but revealed incomplete protecivity against challenge. In swine, the combination of rMCP and gP50 showed the higher serum neutralizing antibody titers and cellular immune responses than rMCP alone. However, the protectivity was lower in comparison with the commercial gI-deleted inactivated vaccine. We expect these results to contribute to characterization of MCP gene of Korean isolate of PRV and to ultilize as preliminary information for prodution and evaluation of PRV recombinant subunit vaccines.

무술 스포츠로서의 택견의 정신에 대한 고찰

최무현,김동준 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.3

Accroding to results about the mental a of Takkyun as a martial sport, We can make following conclusions. 1. The modern martial arts are the concept which is added to sports, When we indicated one of them, we use the word, "martial sports". 2. The modern Korean martial ideologies were under the influence of Japanese martail as they are, Japeneses martail ideologies are hierarchism but Korean martial characteristic are democritism. 3. Takkyun keeps Korean essential martial values like them. 4. Takkyun has been industrialized all over the world from long time ago. That is, Takkyun is one of the martial sports. 5. The martial mental of Takkyun has only mutual coexisting and democratic values which are based on the liberty and equality.

노동운동과 정치운동의 맥락적 의존성 : 부마민주항쟁 사례연구

주무현 경상대학교 사회과학연구소 2003 사회과학연구 Vol.21 No.-

I analyse the contextual dependency between labour movement and political movement in the case of the Busan and Masan Areas' democratic movement of 1979. This paper focuses on the concept of political and industrial opportunity structure as a determinant of labour movement an political movement. Busan and Masan Areas' democratic movement depended contextually on the growth and development of the democratic union movement of 1970s. The success of the political movement opened the political opportunity structure of labour movement of 1980 in Korea. At same time Korean labour movement was contextually determined by the political movement as such Busan and Masan Areas' democratic Movement. In conclusion, the success of labour movement will depend on how it use the political opportunity structure in Korea.

원발성 폐암 환자의 생존율에 관한 보고

김현태,이상무,어수택,박춘식,정성환,허승재,남충희,강창희,김용훈 순천향대학교 1994 논문집 Vol.17 No.4

We analysed 404 patients with primary lung carcinoma who were treated at Soonchunhyang University Hospital from July, 1985 to september, 1993 in order to investigate the survival rate and epidemiolgical properties of primary lung cancer. They were 330 males and 74 females. The most prevalent decade was seventh. In terms of cell type, the squamous cell was 225 patients (55%), and adenocarcinoma, small cell, mixed type was 21%, 19%, 4%, respectively. Among non-small cell lung carcinoma, stage Ⅲa was the most prevalent one(92%). In case of small cell carcinoma, the limited stage was 64%. The 12-, 24-, 36- month survival rate of total patients was 57%, 31%, 22%, respectivley and median sruvival time was 15 months. The 36-month survival rate tended to be longer in non-small cell lung carcinoma than that of small cell lung cancer, but there was no difference between two groups, statistically. In non-small cell carcinoma, The 36-month survival rate and meidan survival time were longer in the stage Ⅰ and Ⅱ than those of Ⅲa, Ⅲb, Ⅳ (80% versus 38%, 22%, 0%, p<0.05). According to involvement of lymph node, the 36-month survival rate was longer in NO and N1 than those of N2, N3 (61.9%, 48.7% versus 17.7%, 17.3%, p<0.05). In small cell carcinoma, The 36-month survival rate and median survival rate were higher and longer in limited stage than those of extensive stage(16.1% and 13 month vs 10% and 8 month, p<0.05). In conclusion, we report here the incidence of primary lung carcinoma and the survival rate of paients with primary lung carcinoma who were treated in Soonchunhyang University Hospital.

Baculovirus를 利用한 Canine Parvovirus VP2蛋自質의 發現

朴鍾賢,宋載永,玄芳動,安動濬,姜永源,全茂炯,安壽煥 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-

국내에서 分離된 개파보바이러스주(V20주)의 VP2 遺傳子를 baculovirus system을 이용하여 발현시켜 다음의 結果를 얻었다. 1. 개파보바이러스의 VP2 유전자를 PCR에 의해 增幅하여 1755bp의 VP2遺傳子를 pUC19에 클로닝하여, 클로닝된 遺傳子를 polyhedrin promoter를 가지는 baculovirus expression vector인 pVL1393에 옮겨 VP2 발현벡터인 pVL1393-VP2를 얻을 수 있었다. 2. pVL1393-VP2 plasmid와 baculovirus DNA와의 homologous recombination에 의해 재조합바이러스인 VP2-BV를 얻을 수 있었으며, 그 발현효율은 2.000-5.000 HAU/0.05 ml이었다. 3. 免疫沈澱法에 의해 발현된 단백질은 개파보바이러스의 VP2단백질과 유사한 64 kb에 달하는 것이었으며, 血球凝集能을 지니고 있었다 4. 血球凝集能을 지닌 蛋白質이 여러 陽性血淸에 있어서 抗體수준을 測定할 수 있는지 개파보바이러스항원과의 相關性을 比較한 바 0.94 (n=125. p<0.01)의 相關係數를 보였다 5. 발현 VP2白은 virus-like particles를 形成하였으며, 그 크기로는 개파보바이러스와 비슷한 25 ㎚의 크기를 갖았다. Canine parvovirus(CPV) is a member of autonomous replicating parvoviruses and is aetiologically associated with enteritis and mycoarditis in puppies. The capsids of CPV are composed of three structural proteins: VP1, VP2 and VP3. The VP2 protein is the major component of capsid. The VP2 gene of a canine parvovirus. V20 strain isolated in Korea was cloned into baculovirus expression vector, and subsequently the VP2 protein was expressed by a recombinant baculovirus under the control of polyhedrin promoter. The recombinant VP2 protein expressed in Spodoptera frugiperda 9(Sf9) cells was detected by haemagglutination(HA) test and immunofluorescent antibody assay. Molecular weight of the recombinant VP2 protein expressed was estimated as 64Kd when tested by immunoprecipitation test using anti-CPV monoclonal antibody. In haemagglutination inhibition(Ⅲ) test. 8 HA units of the recombinant VP2 protein antigen was successfully utilized to determine a level of antibody against CPV in various positive sera. The recombinant VP2 protein showed also the capability to form virus like particles similar in size and appearance to the CPV virions.

감정에 대한 단기간의 유산소적 운동 효과

최무현 경남대학교 교육문제연구소 2003 敎育理論과 實踐 Vol.13 No.2

The purpose of this study was to examine the short-term effects of aerobic exercise on psychological emotion. 16 female college students who were divided into two groups, such as experimental (n=8), and control group (n=8) participated in a single-session experiment during which they carry out two 8-min trials of high-load exercise and two 8-min trials of low- load exercise. A Monark Type 864 bicycle ergometer by cycling at 50rpm against a load of 25w or 100w. Emotion was assessed using an adaptation of the POMS (McNair et al., 1981). The conclusions obtained from this study were as follows ; 1. High-load exercise leads to increase in tension/anxiety and fatigue, whereas positive emotion changes were seen following low-load exercise only. 2. No group differences in emotion responses were observed.

일본적 생산시스템의 구조와 국제적 논쟁에 관한 일 고찰

주무현 慶北大學校出版部 1998 經商論集 Vol.26 No.2

점진적인 팔·다리 운동에 따른 혈액 농도의 변화에 관한 연구

최무현 慶南大學校 附設 基礎科學硏究所 1996 硏究論文集 Vol.8 No.-

The purpose of this study was to assess the influence muscle mass has on blood concentration changes during progressive arm and leg exercise. Eight male subjects with a mean (±SD)age of 23±3.1 yr and weight of 70.2±2.7kg, were randomly assigned to complete either arm or leg exercise test using modified Body-Guard Ergometer 990. The alternate activity was performed within 2 weeks. Especially percent changes in blood and plasma volumes were calculated using values Hct(Van Beaumont et al. :1972) and Hct-Hb (D.B. Dill et al. :1974) Linear regression equations were calculated and compared for differences between slopes and intercepts when contrasting arm and leg exercise. The results obtained from this study were as follows: 1. At same level of oxygen uptake, a relative larger percentage decrement of plasma volume occurred for arm compared with leg exercise. 2. There was a linear relationship between the amount of plasma volume lost from the vasculature and the exercise intensity for both arm and leg exercise. 3. Plasma efflux during exercise was related to the mean arterial pressure which was directly related to the relative intensity of the exercise.