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대두 리폭시게나제 효소와 아라키돈산의 반응중 자기불활성화의 요인에 관련된 중간체 생성물
김미리,석대은 ( Mee Ree Kim,Dai Eun Sok ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.4
Gradual decrease of soybean lipoxygenase activity was observed during the prior incubation with arachidonic acid, while preincubation with linoleic acid or 11,14,17-eicosatrienoic acid had no effect on lipoxygenase activity. Among the lipoxygenation products of arachidonic acid, only 15(S)-hydroperoxy eicosatetraenoic acid (15(S)-HPETE) inhibited lipoxygenase in a time-dependent manner. Either further oxygenation products of 15(S)-HPETE or non-enzymatic decomposition products of 15(S)-HPETE had no significant inhibitory effect. The observations that the inhibitory effect of 15(S)-HPETE was transient, and not affected by hydroxy radical scavangers support the assumption that the unstable lipid intermediates produced from further conversion of 15(S)-HPETE may be responsible for the irreversible inhibition of lipoxygenase. In support of the above assumption, 13(S)-hydroperoxy-6, 9, 11-octadecatrienoic acid, which can be further lipoxygenated was also found to inactivate lipoxygenase effectively. However, 13(S)-hydroperoxy-9,11-octadecadienoic acid or 13(S)-hydroperoxy-9,11,15-octadecatrienoic acid was not effective.
김미리,이혜수,Kim, Mee-Ree,Rhee, Hei-Soo 한국식품과학회 1989 한국식품과학회지 Vol.21 No.1
무우의 myrosinase의 특성 규명을 위해 수행한 효소 정제는, 생 무우의 조효소액을 친화성 컬럼 (충진물 : Con A-Sepharose)에 흡착 시킨 후 ${\alpha}-methyl-D-mannoside$로 용출하였을 때 53%의 회수율과 16배의 정제도(S.P.A.=27,900 units/mg)를 나타내었다. 다음 단계로 gel permeation HPLC에 의해 정제한 결과, 약 50%의 최종 회수율과 22배의 정제도(S.P.A.=39,000 units/mg)를 나타내었으며, 전기 영동상 순도가 입증되었다. Gel permeation HPLC에 의해 측정한 분자량은 약 124,000 이었으며, sinigrin을 기질로 하여 측정한 Km, Vmax값은 각각 0.12mM, $40\;{\mu}moles/mg{\cdot}min$이었다. 작용 최적 pH는 6.5, 최적 온도는 $37^{\circ}C$ 이었고 중성용액(pH 6-7)에서는 상온에서 비교적 안정하였으나 산성용액 (pH 4 이하)에서는 불안정하여 효소활성도는 급격히 감소하였다. 효소의 활성화제인 아스코르브산에 의한 최대 활성화 농도는 0.6mM로 약 100배의 효소 활성도 증가효과를 나타내었으며 2mM이상에서는 효소 활성도가 저하되었다. 무우중 myrosinase의 분포는 껍질 부위에 평균 1,333 units/g, 속 부위에 평균 140 units/g으로 껍질 부위가 속 부위에 비해 10배 정도 많은 효소를 함유하고 있었다. The purification of myrosinase from radish roots was performed using Concannavalin A-Sepharose affinity chromatography and gel permeation HPLC, which gave a 22-fold-purification(S.P.A.=39,000 units/mg) with 50% recovery, SDS-polyacrylamide gel electrophoresis showed a single major band, suggestive of a relatively pure myrosinase, and the M.W. of the enzyme determined on gel permeation HPLC was ca. 124K. The enzyme showed on optimum pH of 6.5 and was stable at pH 6 to 7 at room temperature, but unstable below pH 4. The enzyme possessed an optimum temperature of $37^{\circ}C$, and gave a Vmax value of $40\;{\mu}moles/mg{\cdot}min$ and a Km value of 0.12mM for sinigrin. The purified myrosinase was activated maximally by 0.6mM of ascorbic acid, but somewhat inhibited by more than 2 mM ascorbic acid. The activities of myrosinase present in the peelings and the peeled radish amounted to approximately 1,333 units/g and 140 units/g weight, respectively and the peelings contained much more myrosinase activity than the peeled radish.
케일 및 브로콜리잎즙의 함황 향기성분, 일반성분, 무기질, Vitamin C 함량 및 관능적 특성
김미리(Mee-Ree Kim),김진희(Jin-Hee Kim),위대성(Dae-Sung Wi),나종현(Jong-Hyeon Na),석대은(Dai-Eun Sok) 한국식품영양과학회 1999 한국식품영양과학회지 Vol.28 No.6
To utilize the leaves of broccoli, the shape of which was similar with that of kale leaves, as a vegetable juice, the extracts from two types of vegetable leaves were subjected to the analyses of proximate components, volatile sulfur compounds, vitamin C and minerals. The sensory evaluation of the juices, prepared from kale and broccoli leaves, were performed by duo-trio test and scoring test. Among eight varieties of broccoli, four varieties('Pilgrim', 'Greenbelt', 'Salinas' and 'Shasta')were not significantly different from kale(p<0.05), based on the duo-trio sensory test. Sulfur and nitrogen compounds present in the dichloromethane extracts of kale and broccoli leaves 'Pilgrim' were identified as 3-butenyl, allyl, butyl and 4-methylsulfinylbutyl isothiocyanate(sulforaphane), 4,5-epithiovalero, 4,5-epithio-3-hydroxyvalero, benzenepropane, 3-hydroxy-4-propene, 4-methylthio butane, 3-hydroxy-3-phenylpropane, 5-methyIsulfinylpentane, 4-methoxyphenyl-3-hydroxypropane nitrile and dimethyl trisulfide by GC/MSD analysis. Proximate components were observed to be relatively similar between kale and broccoli leaves. Vitamin C and sugar content were higher in broccoli leaves(125~180㎎% and 8~12˚Brix) than in kale(101㎎% and 7˚Brix). In mineral analysis by AA and ICP-Mass, 'Pilgrim' showed a higher content of K, Fe, Mn, Zn and Se. In separate experiment, sensory scores of bitter taste and astringent taste were lower and scores of over-all taste and over-all acceptability, higher in broccoli ('1243') juice than those in kale juice.
김미리(Mee-Ree Kim),서지현(Ji-Hyun Seo),허옥순(Ok-Soon Heo),오상희(Sang-Hee Oh),이기순(Ki-Soon Lee) 한국식품영양과학회 2002 한국식품영양과학회지 Vol.31 No.5
시판되는 식혜의 품질을 가정에서 제조하는 전통의 식혜와 비교하기 위해 시판 식혜 6종에 대하여 이화학적 특성 및 관능적 특성을 분석하였다. 시판식혜 6종은 제조 식혜에 비해 식품표시에 나타난 성분 중 엿기름 함량 및 밥의 함량이 제조식혜에 비해 낮았다. 또한, 시판 식혜 6종은 제조식혜에 비하여 식혜물의 당도(11.6~12.5°Brix) 및 점도(5.33~9.33 cP)는 낮았으나, pH(5.93~6.87), 투명도(9.1~46.5% at 558 nm), L(32~63), a(2.0~4.6) 및 b값(10.5~15.5)이 높았다. 또한, 식혜 밥알의 특성 중 시판식혜는 색상 a 및 b값과 경도, 탄력성 및 씹힘성이 높았다. 또한, 시중에서 널리 유통되어 시판되는 식혜 3종에 대해 관능평가를 실시한 결과, 엿기름 함유 정도, 밥알의 뜨는 정도, 밥알의 양, 밥알의 질감, 밥알의 색, 부재료 함유정도, 전반적인 기호도는 시판 식혜 3종 모두가 제조식혜에 비하여 유의적으로 낮은 점수를 나타내었으나(p<0.05), 시판 B는 맛과 향에 있어서 유의적인 차이가 없었다. 특히 전반적인 기호도는 제조식혜가 5.7점이었으나 시판식혜는 2.7~3.6점으로 매우 낮았다. 이상의 결과로부터 시판 식혜는 전통적으로 가정에서 제조한 식혜와는 차이가 컸으며 특히, 밥의 양과 엿기름함량이 적은데서 기인되는 점도, 맛 등에서 낮은 점수를 받았고, 전반적인 기호도도 낮았으므로 전통의 맛에 더 가까운 식혜생산을 위해 업체에서는 이같은 점들을 고려한다면 더 바람직할 것으로 생각된다. To assess the quality of commercial sikhes, we compared physicochemical and sensory characteristics of commercial sikhe with home-made one. Six different brands and home-made sikhes were analysed for physicochemical (sugar content, acidity, color, viscosity, transparancy, texture of rice) and sensory characteristics (7-point scoring test, 18 experienced panel members). Commercial sikhes (6 different companies) exhibited lower sugar content (11.6~12.5°Brix) and viscosity (5.33~9.33 cP) than home-made one, whereas higher pH (5.93~6.87), transparency (9.1~46.5% at 558 nm), Hunter L, a and b values. In the texture of rice, commercial sikhe showed higher hardness, springiness and chewiness than those of home-made one in. Sensory evaluation showed that malt and rice content, texture and color of rice, and over-all preference for commercial sikhe was significantly lower than those for homemade one (p<0.05). Especially, the scores of over-all preference for 3 commercial sikhes (2.7~3.6) were much lower than that of home-made one (5.7).
연구논문 : 오디박 분말 첨가 초콜릿의 품질 특성 및 항산화성
김미리 ( Mee Ree Kim ),황미현 ( Mee Hyun Hwang ),전혜련 ( Hye Lyun Jeon ),김형돈 ( Hyung Don Kim ),이상원 ( Sang Won Lee ) 한국식품조리과학회(구 한국조리과학회) 2012 한국식품조리과학회지 Vol.28 No.4
Mulberry pomace, a by-product obtained from mulberry juice, has not been utilized as a food material for food processing. In this study, the quality characteristics and antioxidant activities of chocolate to which mulberry pomace has been added were evaluated. Freeze-dried mulberry pomace was added to white chocolate at the level of 0, 0.5, 1.5 or 2.5%, and then the white chocolate ganache was put between two layers of dark chocolate. The moisture content and sweetness were not significantly different among treatments, but reducing sugar content was increased, according to the amount of mulberry pomace powder. The Hunter color L(lightness) and b(yellowness) values decreased, according to the mulberry pomace powder amount, whereas the a(redness) increased. The hardness increased according to the mulberry pomace powder amount. Total phenol and flavonoid contents increased, according to the mulberry pomace powder amount. Antioxidant activities(DPPH and hydroxyl radical scavenging activities) also increased, according to the mulberry pomace powder amount. The result of the sensory intensity test revealed that the scores of color, mulberry taste, hardness and chewiness were the highest in the 2.5% mulberry pomace powder added chocolate. Sensory preference test results showed that chocolate added with 1.5% mulberry pomace powder had the highest scores in color, flavor, taste, texture and overall preference. It is suggested that chocolate to which mulberry pomace powder has been added may be a functional chocolate, with high antioxidant activity.