캡슐소재에 따른 멸치어유의 미세캡슐화

임상빈,좌미경,송대진 濟州大學校 産業技術硏究所 1999 산업기술연구소논문집 Vol.10 No.1

Microencapsulation of anchovy oil with different wall materials such as sodium alginate. chitosan and β-cyclodextrin was investigated. Microencapsulation was accomplished by ejecting an oil/water emulsion into a dispersion fluid under high pressure through an orifice. As a dispersion fluid, 0.2% calcium lactate was appropriate for sodium alginate, while 0.18 sodium hydroxide for chitosan in terms of capsule size and distribution, and emulsion stability. β -cyclodextrin formed inclusion complex with anchovy oil. The microcapsules were stored at 22 and 30℃ for 8 days, and their stability was tested. The acid value remained unchanged and the peroxide value was not detected.

초임계 이산화탄소에 의한 멸치어유의 추출

임상빈,좌미경,송대진 濟州大學校 産業技術硏究所 1998 산업기술연구소논문집 Vol.9 No.1

Anchovy oil was extracted by supercritical CO_(2). and extraction yield and fatty acid composition were measured in the extract and residual fractions. EPA and DHA were 7.6~13.3% and 16.2~18.3% for the original anchovy oil. The extraction yield of anchovy oil increased as the extraction temperature decreased. Essentially no great differences were noticed in the fatty acid composition of the oil extracted under different extraction temperatures. Anchovy oil mixed with silver nitrate-coated silica gel was extracted by only SC-CO_(2)for 2 hr and by ethyl acetate-modified SC-CO_(2)for 2 hr at 60℃/276 bar. This produced higher extraction yield and higher concentration of EPA and DHA in the residual fraction. Starting with anchovy oil containing 13.3% EPA and 16.2% DHA. the residue fraction containing 23.9% EPA and 31.1% DHA was obtained.

Improved Micropropagation of Root Chicory, Cichorium intybus L. var. sativus.

Lim,Jung-Dae,Yang,Deok-Chun,Lee,Hyeon-Yong,Kim,Jong-Dai,Lee,Jin-Ha,Sung,Eun-Soo,Yu Chang-Yeon 한국자원식물학회 2004 Plant Resources Vol.7 No.1

The establishment of an efficient protocol for plant regeneration and micropropagation from leaf explant cultures of Chicory, Cichorium intybus L. var. sativus. is reported. Callus formation rate appeared 100% from explant in all growth regulators, but calli formed in the prensence of naphthaleneacetic acid (NAA) were appeared very compact and non-embryogenic state. The regenerated shoots were obtained from leaf explant cultures on solid MS medium containing different concentrations of cytokinins and auxin. The highest number of shoots (5.7) per explant and shoot growth (2.8cm) was obtained on MS medium containing 0.1 mg BAP L^-1 and 0.1 mg NAA L^-1. Indole acetic acid was the most suitable auxin for root formation among three auxins tested. 2,4-D had no effect on shoot and root formation.

Identification of Novel Endo-β-1,4-glucanase Isoforms fromBursaphelenchus Species (Nemtoda: Aphelenchoididae)

Dae-Weon Lee,Il Sung Moon,강재순,Hyun Jung Choe,최재영,제연호,이시혁,Hye Rim Han,Kook-Jin Lim6,Young Ho Koh 한국응용곤충학회 2007 Journal of Asia-Pacific Entomology Vol.10 No.2

Bursaphelenchus xylophilus has been recognized as a causal pathogen of pine wilt disease (PWD). In order to identify relevant molecular biomarkers, we selected a variable region of endo-β-1,4-glucanase (β14-gcn) which was cloned from the cDNA of B. xylophilus and B. mucronatus. We then identified three novel β14-gcn isoforms: Bm-β14-gcn and Bm-β14-gcni from B. mucronatus, and Bx-β14-gcn from B. xylophilus. Bm-β14-gcn and Bx-β14-gcn were found to be identical, whereas Bm-β14-gcni was unique to B. mucronatus. These isoforms are Bursaphelenchus-specific and may be employed as molecular markers for the diagnosis of PWD. Our phylogenetic analysis showed that these β14-gcns from Bursaphelenchus species were associated most closely with fungal β14-gcns.

초피 추출물의 수율 및 항균활성

임상빈,송대진,좌미경,고정삼 濟州大學校工科大學産業技術硏究所 1995 尖端技術硏究所論文集 Vol.6 No.-

초피를 유기용매 및 초임계이산화탄소와 보조용매로서 에탄올로 추출하여 각각의 추출수율을 측정하고, 이들 추출물들의 항균활성을 검색하였다. 유기용매별로 추출하여 각각의 추출수율을 측정한 결과, 증류수인 경우 20%로서 가장 높았고, 나머지 헥산, 에테르, 에탄올인 경우 3%이하로 매우 적었다. 또한 추출 용매로 초임계이산화탄소-에탄올을 병용했을 때 초피의 추출수율과 용해도는 초임계이산화탄소만을 사용했을 때보다 1.4배 이상 높았다. 용매별 추출물들의 항균성을 검색한 결과 모든 추출물들이 B.cereus에 대해 항균성을 나타내었는데, 특히 에탄올, 에테르 추출물들의 항균성이 더 큰 것으로 나타났다. 초임계이산화탄소-에탄올 추출물은 초임계이산화탄소만을 사용한 추출물과 거의 동일한 항균성을 나타내었다. Extraction yields of Zanthoxylum piperitum by organic solvents and supercritical carbon dioxide(SC-CO₂) with and without ethanol as a cosolvent, and antimicrobial activities of the extracts against Bacillus cereus were determined. Extraction yield by distilled water was the highest, 20%, while those by ethanol, ethyl ether and hexane were less than 3%. Extraction yield by SC-CO₂ with ethanol was 1.42 times greater than that by only SC-CO₂ Ethyl ether- and ethanol-extracts had potent antimicrobial activities against B.cereus. Extraction yield by SC-CO₂ with ethanol showed almost the same degree of microbial growth inhibition as that by only SC-CO₂.

염농도에 따른 멸치젓의 품질 변화

임상빈,오창경,송대진 제주대학교 산업기술연구소 2000 尖端技術硏究所論文集 Vol.11 No.1

General composition, pH, salinity, peroxide value, TBA value, volatile basic nitrogen(VBN) and amino nitrogen were measured for fermented anchovy with different salt content. Moisture, crude protein, crude lipid and ash of raw anchovy were 75.9, 18.2, 2.36 and 3.9%, respectively. Moisture of fermented anchovy decreased as the increase of salt added. Crude protein and crude lipid were the highest in 12% salt fermented anchovy. Ash of fermented anchovy increased as the increase of salt content. pH and salinity of raw anchovy were 7.0 and 0.61%, respectively. pH of fermented anchovy were 6.0 regardless of salt concentration. Peroxide values increased drastically with the increase of salt added. TBA was the lowest and amino nitrogen was the highest in 8% salt fermented anchovy.

초임계 이산화탄소에 의한 멸치어유의 추출

임상빈,좌미경,송대진 濟州大學校 工科大學 産業技術硏究所 1998 尖端技術硏究所論文集 Vol.9 No.1

고품질의 고도불포화지방산 제품을 제조할 목적으로, 멸치어유를 초임계 이산화탄소로 추출분획한 후 각 분획들의 추출수율과 지방산 조성을 측정하였다. 멸치어유의 주요 지방산은 palmitic acid. DHA, EPA, palmitoleic acid. myristic acid. oleic acid 였다. 멸치어유의 추출수율은 동일압력에서 추출시간의 증가에 따라 감소하였으며, 40과 50℃에서는 추출온도의 증가에 따라 감소하였으나, 60℃에서는 50℃ 보다 다소 높았다. 동일온도에서 C14와 C16 지방산들은 추출물 분획에 농축되었고, 나머지 지방산들은 추출 잔류물 분획에 농축되었다. EPA는 분획들간에 거의 일정한 경향을 보였으나, DHA는 추출잔류물 분획에 농축되었다. Silver nitrate를 silica gel에 코팅시킨 후 어유와 혼합하여 추출 2시간 동안은 초임계 이산화탄소로만으로 추출하고, 그 후 보조용매로 ethyl acetate를 첨가하여 2시간 동안 추출하였을 때 추출물 분획에서의 저급지방산인 C14:0은 추출잔류물에 비하여 5.0∼6.4배 농축되었고, C16:0은 1.9∼2.0배 농축된 반면, 추출잔류물 분획에서 EPA와 DHA는 23.9와 31.6%로 원료어유의 13.3과 16.2%에 비하여 1.8배와 1.9배 농축되었다. Anchovy oil was extracted by supercritical CO₂, and extraction yield and fatty acid composition were measured in the extract and residual fraction. EPA and DHA were 7.6∼13.3% and 16.2∼18.3% for the original anchovy oil. The extraction yield of anchovy oil increased as the extraction temperature decreased. Essentially no great differences were noticed in the fatty acid composition of the oil extracted under different extraction temperatures. Anchovy oil mixed with silver nitrate-coated silica gel was extracted by only SC-CO₂ for 2 hr and by ethyl acetate-modified SC-CO₂ for 2 hr at 60℃/276 bar. This produced higher extraction yield and higher concentration of EPA and DHA in the residual fraction. Starting with anchovy oil containing 13.3% EPA and 16.2% DHA, the residue fraction containing 23.9% EPA and 31.1% DHA was obtained.

염농도에 따른 멸치젖의 품질 변화

임상빈,오창경,송대진 濟州大學校 産業技術硏究所 2000 산업기술연구소논문집 Vol.11 No.1

General composition, pH. salinity. peroxide value. TBA value. volatile basic nitrogen(VBN) and amino nitrogen were measured for fermented anchovy with different salt content. Moisture. crude protein, crude lipid and ash of raw anchovy were 75.9, 18.2, 2.36 and 3.9%. respectively. Moisture of fermented anchovy decreased as the increase of salt added. Crude protein and crude lipid were the highest in 12% salt fermented anchovy. Ash of fermented anchovy increased as the increase of salt content. pH and salinity of raw anchovy were 7.0 and 0.61%. respectively. pH of fermented anchovy were 6.0 regardless of salt concentration. Peroxide values increased drastically with the increase of salt added. TBA was the lowest and amino nitrogen was the highest in 8% salt fermented anchovy.

혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement

임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.

Effect of Photoperiod, Temperature and True-leaf Stage in Bolting Rate of Chicory (Cichorium intybus L. var. sativus)

Lim,Jung-Dae,Seo,Jeong-Sik,Lee,Hyeon-Yong,Kim,Jong-Dai,Lee,Jin-Ha,Yu,Chang-Yeon 한국자원식물학회 2004 Plant Resources Vol.7 No.1

Root chicory (Cichorium intybus L. var. sativus) is potential alternative medicinal and sugar crop which accumulates a high amount of linear polyfructan, inulin in its roots. A problem in root production is that over-wintered stock plants often flower. Once the plant becomes reproductive, stem elongation and root growth slows and floral buds arise from every node, rendering the plants useless for propagation. The objectives of this research was to examine the effectiveness of manipulating environmental factors containing photoperiod, temperature and number of leaf states. The experiment was performed in growth chamber to create two photoperiods (8 h, and 16 h) with three temperature regimes (5℃/3℃, 10℃/8℃, and 15℃/13℃ day/night temperature) for a total of six treatments on three type of true-leaf stage of plant. Data of bolting rate, shoot and root length, shoot and fresh weight was invetigated in each treatments. This is the first report on changes in bolting rate and shoots and roots production during a whole growing season and differences in the effect of cold and photoperiod treatment depending on the true-leaf stage of plant.