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한상현,조인철,이성수,Leoncia Tandang,이항,오홍식,제주대학교,김병수,오문유 한국통합생물학회 2007 Animal cells and systems Vol.11 No.2
The nucleotide sequences of a male-specificmarker sex determining region Y (SRY) gene and amitochondrial cytochrome B (CYTB) gene were characterizedand analyzed to establish a molecular method for identificationof species and sex of Korean roe deer (Capreolus pygargustianschanicus SRY sequencesshowed very similar result to those reported in Moose (Alcesalces) and Reindeer (Rangifer tarandus), both of which had95.9% similarity in identity. CYTB genes were very similar tothose reported in Siberian roe deer (C. pygargus pygargus)which had 98.6% similarity and not to European roe deer (C.capreolus), suggesting that the DNA samples tested were ofSiberian roe deer lineage. Polymerase chain reaction(PCR)-based sex typing successfully discriminated betwencarcasses of male and female roe deer. Males had SRYband on agarose gels and females did not. The result of thismolecular sex typing provided similar information with thatobtained by genital organ observation. Therefore, thismolecular method using male specific marker SRY andmitochondrial CYTB genes would be very useful foridentification of the species and sex of the carcass remainsof roe deer.
Han, Sang-Hyun,Cho, In-Cheol,Lee, Sung-Soo,Tandang, Leoncia,Lee, Hang,Oh, Hong-Shik,Kim, Byoung-Soo,Oh, Moon-You The Korean Society for Integrative Biology 2007 Integrative biosciences Vol.11 No.2
The nucleotide sequences of a male-specific marker sex determining region Y (SRY) gene and a mitochondrial cytochrome B (CYTB) gene were characterized and analyzed to establish a molecular method for identification of species and sex of Korean roe deer (Capreolus pygargus tianschanicus). Similarity search result of SRY sequences showed very similar result to those reported in Moose (Alces alces) and Reindeer (Rangifer tarandus), both of which had 95.9% similarity in identity. CYTB genes were very similar to those reported in Siberian roe deer (C. pygargus pygargus) which had 98.6% similarity and not to European roe deer (C. capreolus), suggesting that the DNA samples tested were of Siberian roe deer lineage. Polymerase chain reaction (PCR)-based sex typing successfully discriminated between carcasses of male and female roe deer. Males had SRY band on agarose gels and females did not. The result of this molecular sex typing provided similar information with that obtained by genital organ observation. Therefore, this molecular method using male specific marker SRY and mitochondrial CYTB genes would be very useful for identification of the species and sex of the carcass remains of roe deer.
Jun, Neung-Jae,Jang, Ki-Chang,Kim, Seong-Cheol,Moon, Doo-Young,Seong, Ki-Cheol,Kang, Kyung-Hee,Tandang, Leoncia,Kim, Phil-Hoon,Cho, So-Mi K.,Park, Ki-Hun The Korean Society for Applied Biological Chemistr 2007 Journal of Applied Biological Chemistry (J. Appl. Vol.50 No.4
The contents of total phenol and total flavonoid of artichoke (Cynara scolymus L.) were measured. The antioxidant activity of the artichoke was evaluated based on its potential as a scavenging the ABTS radical. These results showed the antioxidant activity of artichoke has a close relationship with the total flavonoid content. The compound showing antioxidant activity was isolated from the artichoke by repeated column chromatography and recrystallization. Based on the spectrometric studies, the compound was identified as 1,3-dicaffeoylquinic acid, known as cynarin. The content of cynarin from heads and leafs of the artichoke determined by $C_{18}$ reversed phase HPLC (high-performance liquid chromatography) coupled with photodiode array detector was 10.15 and 0.67 mg/g, respectively. This compound showed potent antioxidant activities against DPPH and ABTS radicals ($EC_{50}$ = 14.09 and 28.85 ${\mu}M$, respectively).