식이섬유질원이 포함된 식이가 흰쥐의 장내 균총조성에 미치는 영향

이상선,이현아,김연희 漢陽大學校 韓國生活科學硏究所 1996 韓國 生活 科學 硏究 Vol.- No.14

This study was performed to investigate the influence of dietary fiber from the whole food on the composition of intestinal microflora in rats. The fiber sources of experimental diets were prepared by drying and milling of small water dropwort(WD), mugwort(MW), butterbur(BB) and apple(AP). Each fiber sources was mixed at the 15% level of the diet. The level of dietary fiber in WD, MW, BB diets was simillar but that in AP diet was slightly low. Male rats of Sprague-Dawley strain were blocked into 5 groups: WD, MW, BB, AP, FF(fiber free), The animal was fed ad libitum each of experimental diets for 37 days. Total viable counts and the numbers of Lactobacillus, Bifidobacterium, Bacteroidaceae, Eubacterium, Peptococcaceae, Streptococcus, Clostridium, Staphylococcus, E. coli were determined by nonselective and various selective media. Food intake of AP and FF groups were lower than the other groups, FF group was lower than the other groups in flood efficiency ratio. Total viable counts were the highest in FF group. The number of Bacteroides, Eubacterium were higher in AP group compared with other groups and that of streptococcus was lower in MW, BB, AP groups than others. The number of Lactobacillus was higher in WD, MW, BB groups than the other groups and that of Bifidobacterium was higher in BB, AP groups. A decrease in the intestinal population of Clostridium was shown in MW, BB groups. These findings suggests that the samll water dorpwort, mugwort butterbur are effective in improving intestinal flora of rats.

브라켓 부착시 실런트 레진이 법랑질 탈회에 미치는 영향

윤영아,이기헌,황현식,홍석진 전남대학교 치의학연구소 2002 구강과학 Vol.14 No.1

The purpose of this study was to evaluate the effects of a sealant resin on enamel demineralization in orthodontic bracket bonding. The forty eight extracted sound bovine teeth was subdivided into four groups and treated with Phase Ⅱ^(r) on the surface. Group 1 was not treated, Group 2 was acid etched with 37% phosphoric acid for 30 seconds, Group 3 was sealant applied after acid etching and Group 4 was resin paste applied after acid etching and sealant application. Each group was demineralized in artificial caries solution. Demineralized enamel depth was measured using by confocal laser scanning microscopy. The results were as follows. 1. The mean demineralized enamel depth was Group 1 (47.4㎛), Group 2 (61.8㎛), Group 3 (13.9㎛), Group 4 (8.2㎛). 2. The demineralized enamel depth was increased in Group 2 than Group 1 (p<0.05). 3. The demineralized enamel depth was reduced in Group 3 than Group1 and Group 2 with statistically significant differences (p<0.05). 4. Group 4 was very little demineralized. The results of the present study indicate that sealant application is useful for reducting enamel demineralization in orthodontic bracket bonding.

모 컴퓨터 회사 근로자 건강증진 사업 전개를 위한 수요조사

임현우,안병용,최환석,박정일,김경수,이강숙,김정아,구정완 가톨릭대학산업의학쎈타 산업의학연구소 1997 韓國의 産業醫學 Vol.36 No.4

Before conducting the health promotion program, we surveyed the health risk appraisal(HRA) of 210 workers (186 men and 24 women) in a computer company, for the need assessment, selection of target population, priority setting, and as a standard for comparision after performing the program. The smokers were 115(88.6%), and drinkers (over 1 bottle of Soju per week) were 91(44.8%) in men. Entire women and 148(78.0%) men never or irregularly exercise. Type A personality were 73(39.2%) in men, 8(33.3%) in women, and 26(14.0%) of men and 6(25.0%) of women were high stress risk group. 53(25.2%) of men and 2(8.3%) of women were obese (BMI ≥25kg/m²), and workers who consumed exess of total calory compared with recommendation were 142(76.3%) in men and 18(75%) of women. By the health risk appraisal, it was suggested that smoking cessation program, drinking counselling, exercise prescription, stress management, and weight control program should be conducted effectively for the high risk group.

보건관리 대행사업장 근로자에서 건강증진에 대한 5년 사이의 지식, 태도 및 실천의 변화

임현우,김정아,안병용,박정일,구정완,이강숙 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.2

Before and after conducting the health promotion program by group occupational health service during 5 years, we surveyed knowledge, attitude and practice about general health (30 items) and occupational health(30 items) among 25 small and medium scale industry workers(before: 355 workers, after: 279 workers) for evaluation of effectiveness. The scores of knowledge for smoking, drinking, cholesterol, mental stress, management of hypertension, and risks in younger or women workers were significantly increased after conducting group occupational health service. The scores of attitude for controlling of fatty food consumption and cleaning workplace and bathing for health and that of practice for taking the periodic health examination and checking blood pressure were significantly increased but most of items in attitude and practice didn't be changed after services. According to sex, the scores of men were significantly increased in knowledge of general health, but there were not significant differences in age, job status and work duration group. In conclusion, through the group occupational health service during 5 years, only the scores of knowledge in general health were increased. It suggested that the effective program which can change workers' attitude and practice for health promotion, should be developed and conducted in small and medium scale industry workers.

요양급여 적정성 평가체계 혁신 중장기 계획

김상지,전숙경,이창원,정영애,안재영,안유미,권아영,임상희,최정남,윤미선,변의형,조미현 건강보험심사평가원 2021 HIRA RESEARCH Vol.1 No.2

Inflammation-Modulated Metabolic Reprogramming Is Required for DUOX-Dependent Gut Immunity in <i>Drosophila</i>

Lee, Kyung-Ah,Cho, Kyu-Chan,Kim, Boram,Jang, In-Hwan,Nam, Kibum,Kwon, Young Eun,Kim, Myungjin,Hyeon, Do Young,Hwang, Daehee,Seol, Jae-Hong,Lee, Won-Jae Elsevier 2018 Cell host & microbe Vol.23 No.3

<P><B>Summary</B></P> <P>DUOX, a member of the NADPH oxidase family, acts as the first line of defense against enteric pathogens by producing microbicidal reactive oxygen species. DUOX is activated upon enteric infection, but the mechanisms regulating DUOX activity remain incompletely understood. Using <I>Drosophila</I> genetic tools, we show that enteric infection results in “pro-catabolic” signaling that initiates metabolic reprogramming of enterocytes toward lipid catabolism, which ultimately governs DUOX homeostasis. Infection induces signaling cascades involving TRAF3 and kinases AMPK and WTS, which regulate TOR kinase to control the balance of lipogenesis versus lipolysis. Enhancing lipogenesis blocks DUOX activity, whereas stimulating lipolysis via ATG1-dependent lipophagy is required for DUOX activation. <I>Drosophila</I> with altered activity in TRAF3-AMPK/WTS-ATG1 pathway components exhibit abolished infection-induced lipolysis, reduced DUOX activation, and enhanced susceptibility to enteric infection. Thus, this work uncovers signaling cascades governing inflammation-induced metabolic reprogramming and provides insight into the pathophysiology of immune-metabolic interactions in the microbe-laden gut epithelia.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Enteric infection stimulates pro-catabolic signaling in the gut epithelia </LI> <LI> Infection induces metabolic reprogramming of gut epithelia toward lipid catabolism </LI> <LI> Gut metabolic reprogramming is required for DUOX activity and resistance to infection </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>

Effects of warming and eutrophication on coastal phytoplankton production

Lee, Kyung Ha,Jeong, Hae Jin,Lee, Kitack,Franks, Peter J.S.,Seong, Kyeong Ah,Lee, Sung Yeon,Lee, Moo Joon,Hyeon Jang, Se,Potvin, Eric,Suk Lim, An,Yoon, Eun Young,Yoo, Yeong Du,Kang, Nam Seon,Kim, Kwan Elsevier Science B.V., Amsterdam. 2019 HARMFUL ALGAE Vol.81 No.-

<P><B>Abstract</B></P> <P>Phytoplankton production in coastal waters influences seafood production and human health and can lead to harmful algal blooms. Water temperature and eutrophication are critical factors affecting phytoplankton production, although the combined effects of warming and nutrient changes on phytoplankton production in coastal waters are not well understood. To address this, phytoplankton production changes in natural waters were investigated using samples collected over eight months, and under 64 different initial conditions, established by combining four different water temperatures (i.e., ambient T, +2, +4, and + 6 °C), and two different nutrient conditions (i.e., non-enriched and enriched). Under the non-enriched conditions, the effect of warming on phytoplankton production was significantly positive in some months, significantly negative in others, or had no effect. However, under enriched conditions, warming affected phytoplankton production positively in all months except one, when the salinity was as low as 6.5. These results suggest that nutrient conditions can alter the effects of warming on phytoplankton production. Of several parameters, the ratio of initial nitrate concentration to chlorophyll <I>a</I> concentration [NCCA, μM (μg L<SUP>−1</SUP>)<SUP>−1</SUP>] was one of the most critical factors determining the directionality of the warming effects. In laboratory experiments, when NCCA in the ambient or nutrient-enriched waters was ≥1.2, warming increased or did not change phytoplankton production with one exception; however, when NCCA was <1.2, warming did not change or decreased production. In the time series data obtained from the coastal waters of four target countries, when NCCA was 1.5 or more, warming increased phytoplankton production, whereas when NCCA was lower than 1.5, warming lowered phytoplankton production, Thus, it is suggested that NCCA could be used as an index for predicting future phytoplankton production changes in coastal waters.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Four temperatures and two nutrient conditions in 8 months were combined. </LI> <LI> Under non-enriched conditions, warming affected positively, negatively, or negligibly. </LI> <LI> Under the enriched conditions, warming in all months except one affected positively. </LI> <LI> Thus, nutrient conditions can alter warming effects on phytoplankton production. </LI> <LI> Ratio of initial nitrate to chlorophyll-a concentration was a critical factor. </LI> </UL> </P>

Improved real-time PCR method for quantification of the abundance of all known ribotypes of the ichthyotoxic dinoflagellate <i>Cochlodinium polykrikoides</i> by comparing 4 different preparation methods

Lee, Sung Yeon,Jeong, Hae Jin,Seong, Kyeong Ah,Lim, An Suk,Kim, Ji Hye,Lee, Kyung Ha,Lee, Moo Joon,Jang, Se Hyeon Elsevier Science B.V., Amsterdam. 2017 HARMFUL ALGAE Vol.63 No.-

<P><B>Abstract</B></P> <P>Red tides by the ichthyotoxic dinoflagellate <I>Cochlodinium polykrikoides</I> have caused large scaled mortality of fish and great loss in aquaculture industry in many countries. Detecting and quantifying the abundance of this species are the most critical step in minimizing the loss. The conventional quantitative real-time PCR (qPCR) method has been used for quantifying the abundance of this species. However, when analyzing >500 samples collected during huge <I>C. polykrikoides</I> red tides in South Sea of Korea in 2014, this conventional method and the previously developed specific primer and probe set for <I>C. polykrikoides</I> did not give reasonable abundances when compared with cell counting data. Thus improved qPCR methods and a new specific primer and probe set reflecting recent discovery of 2 new ribotypes have to be developed. A new species-specific primer and probe set for detecting all 3 ribotypes of <I>C. polykrikoides</I> was developed and provided in this study. Furthermore, because the standard curve between cell abundance and threshold cycle value (Ct) is critical, the efficiencies of 4 different preparation methods used to determine standard curves were comparatively evaluated. The standard curves were determined by using the following 4 different preparations: (1) extraction of DNA from a dense culture of <I>C. polykrikoides</I> followed by serial dilution of the extracted DNA (CDD method), (2) extraction of DNA from each of the serially diluted cultures with different concentrations of <I>C. polykrikoides</I> cultures (CCD method), (3) extraction of DNA from a dense field sample of <I>C. polykrikoides</I> collected from natural seawater and then dilution of the extracted DNA in serial (FDD method), and (4) extraction of DNA from each of the serially diluted field samples having different concentrations of <I>C. polykrikoides</I> (FCD method). These 4 methods yielded different results. The abundances of <I>C. polykrikoides</I> in the samples collected from the coastal waters of South Sea, Korea, in 2014–2015, obtained using the standard curves determined by the CCD and the FCD methods, were the most similar (0.93–1.03 times) and the second closest (1.16–1.33 times) to the actual cell abundances obtained by enumeration of cells. Thus, our results suggest that the CCD method is a more effective tool to quantify the abundance of <I>C. polykrikoides</I> than the conventional method, CDD, and the FDD and FCD methods.</P>

Brain ischemic preconditioning protects against moderate, not severe, transient global cerebral ischemic injury

Lee, Jae-Chul,Shin, Bich-Na,Cho, Jeong Hwi,Lee, Tae-Kyeong,Kim, In Hye,Noh, YooHun,Kim, Sung-Su,Lee, Hyang-Ah,Kim, Young-Myeong,Kim, Hyeyoung,Cho, Jun Hwi,Park, Joon Ha,Ahn, Ji Hyeon,Kang, Il Jun,Hwan Springer-Verlag 2018 Metabolic brain disease Vol.33 No.4

Characteristics of Pediatric Inflammatory Bowel Disease in Korea: Comparison with EUROKIDS Data

( Hyeon Ah Lee ),( Jung Yoon Suk ),( Sung Youn Choi ),( Eun Ran Kim ),( Young-ho Kim ),( Chang Kyun Lee ),( Kyu Chan Huh ),( Kang Moon Lee ),( Dong Il Park ) 대한소화기학회 2015 Gut and Liver Vol.9 No.6

Background/Aims: Pediatric inflammatory bowel disease (IBD) has been increasing worldwide. The characteristics of pediatric-onset IBD have mainly been reported in Western countries. We investigated the clinical characteristics of pediatric IBD in Korea and compared these with the data from the 5-year European multicenter study of children with new-onset IBD (EUROKIDS registry). Methods: Children who were diagnosed with IBD between July 1987 and January 2012 were investigated at five Korean university hospitals. Their clinical characteristics were retrospectively evaluated by medical record review. The results were compared with the EUROKIDS data. Results: A total of 30 children with Crohn’s disease (CD) and 33 children with ulcerative colitis (UC) were enrolled. In comparison with the EUROKIDS group, Korean pediatric IBD patients showed a male predominance (86.7% vs 59.2%, p=0.002 in CD, 75.8% vs 50%, p=0.003 in UC). Korean pediatric CD patients had a higher prevalence of terminal ileal disease (36.7% vs 16.3%, p=0.004) and perianal disease (33.3% vs 8.2%, p<0.001) than patients in the EUROKIDS group. Korean pediatric UC patients had a higher prevalence of proctitis than patients in the EUROKIDS group. Conclusions: Our results suggest that the characteristics of Korean pediatric IBD patients and European pediatric IBD patients may be different. (Gut Liver 2015,9:756-760)