Nutlin-3 induces HO-1 expression by activating JNK in a transcription-independent manner of p53

CHOE, YUN-JEONG,LEE, SUN-YOUNG,KO, KYUNG WON,SHIN, SEOK JOON,KIM, HO-SHIK Spandidos Publications 2014 International journal of oncology Vol.44 No.3

A recent study reported that p53 can induce HO-1 by directly binding to the putative p53 responsive element in the HO-1 promoter. In this study, we report that nutlin-3, a small molecule antagonist of HDM2, induces the transcription of HO-1 in a transcription-independent manner of p53. Nutlin-3 induced HO-1 expression at the level of transcription in human cancer cells such as U2OS and RKO cells. This induction of HO-1 did not occur in SAOS cells in which p53 was mutated and was prevented by knocking down the p53 protein using p53 siRNA transfection, but not by PFT-alpha, an inhibitor of the transcriptional activity of p53. Accompanying HO-1 expression, nutlin-3 stimulated the accumulation of ROS and the phosphorylation of MAPKs such as JNK, p38 MAPK and ERK1/2. Nutlin-3-induced HO-1 expression was suppressed by TEMPO, a ROS scavenger, and chemical inhibitors of JNK and p38 MAPK but not ERK1/2. In addition, nutlin-3-induced phosphorylation of JNK but not p38 MAPK was inhibited by TEMPO. Notably, the levels of nutlin-3-induced ROS were correlated with the mitochondrial translocation of p53 and this induction was prevented by PFT-beta, an inhibitor of the mitochondrial translocation of p53. Consistent with the effect of the ROS scavenger and MAPK inhibitors, PFT-beta reduced HO-1 expression and the phosphorylation of JNK induced by nutlin-3. In the experiments of analyzing cell death, the knockdown of HO-1 augmented nutlin-3-induced apoptosis. Collectively, these results suggest that nutlin-3 induces HO-1 expression via the activation of both JNK which is dependent on ROS generated by p53 translocated to the mitochondria and p38 MAPK which appears to be stimulated by a ROS-independent mechanism, and this HO-1 induction may inhibit nutlin-3-induced apoptosis, constituting a negative feedback loop of p53-induced apoptosis.

CMOS 연산 증폭기 및 비교기 회로의 자동 설계

신현우,김천호,김득경,신창호,신경욱 國立金烏工科大學校附設生産技術硏究所 1994 産業技術開發硏究 Vol.10 No.-

The paper describes a CMOS analog circuit design tool, K_ACD(Kumoh_Analog Circuits Designer), and design results. The K_ACD was developed for automatic design of fundamental analog circuit blocks such as comparator, unbuffered OP AMP, and buffered OP AMP. For given specifications and process parameters, it computes bias currents, transistor sizes, voltage gain, and power dissipation, then SPICE simulations are automatically carried out. Some design examples show usefulness of the K_ACD in analog circuit designs.

한국형 고속전철 집전 시스템의 동적설계에 관한 연구

허신(Hur Shin),경진호(Kyung Jin-Ho),송달호(Song Dahl-Ho) 한국철도학회 1998 한국철도학회 학술발표대회논문집 Vol.- No.-

The dynamic performance design of catenary-pantograph system which collects current for the next generation Korean high speed train(KHST) was considered. Used was the same dynamic model of the catenary-pantograph system as that of TGV-K which will be introduced for Kyung-bu corridor. Using the model, sensitivity analysis for design variables were made to improve dynamic performance of KHST system. The results of sensitivity analysis and performance improvement are as follows: (1) It was found that aerodynamic force, tension of contact wire, mass of contact strip, mass of supporting contact strip, mass of clamp, mass of steady arm, and stiffness of plunger were the design variables most influencing the dynamic performance of the system. (2) Pantograph with reductions of 20% aerodynamic force, 34% weight of supporting contact strip, 20% spring constant of plunger, and 34% equivalent mass of steady arm was very possible system for the KHST which will be running at maximum operating speed 350 ㎞/h.

전자선으로 전처리한 하수슬러지의 혐기성소화공정에 관한 연구

신경숙,강호 충남대학교 환경문제연구소 2000 環境硏究 Vol.18 No.-

To treat a large amount of sewage sludge generated from wastewater treatment plants, various pretreatment processs have been developed for the subsequent anaerobic digestion. As one of the processes, the pretreatment with electron beam irradiation has been investigeted. The SCFMRS were run to evaluate their reactor performance of sludge with and without irradiation by electron beam. The dewaterbility of digested sludge, which is derived from SCFMR, was also measured by Specific Resistance to Filtration(SRF) and Capillary Sunction Time(CST). The biogas productivity of SCFMR fed with 6kGy irradiated sludge showed 0.10∼0.14m3/m3-d at the HRT of 20days. It appeared 3∼5 times higher than that of unirradiated sludge in SCFMR. Consequently, TVS and COD removal efficiencies in the SCFMR fed with 1∼6kGy irradiated sludge were 30∼55% and 32∼52%, respectively, which were 20∼35% higher than those of unirradiated sludge as a function of irradiated dosages. The SRF of digested sludge pretreated with electron beam is 2.2 times lower than that of irradiated sludge. In conclusion, electron beam treatment on sewage sludge could accerlerate organic degradation rate and also enhance the dewaterbility of divested sludge.

18S-srRNA 분석에 의한 Acantbamoeba 한국 분리주들의 분류 검토

신호준,지영진,조명수,김형일,박연희,임경일 아주대학교 의과학연구소 1998 아주의학 Vol.3 No.2

Acanthamoeba spp. spread in soil, ponds, air and swimming pool, cause a granulomatous amoebic meningitis and amoebic keratitis in human and experimental animals. Their classification had depended upon morphological characteristics and physiological conditions such as thermophilic status and generation times. However, because amoeba species showed the morphological and physiological diversity in isolates, many attempts have been made on identification of Acanthamoeba spp. Restriction fragments length polymorphism (RFLP) analysis of IBS-small subunit ribosomal RNA (srRNA) was an useful tool for classification among morphologically and genetically closely-related species. In this study, 18S-srDNAs of amoebae were amplified by PCR with primer encoding 18S-srRNA gene and digested with restriction endonudeases, and RFLP analysis was applied on classification of Acanthamoeba Korean isolates (YM-4, YM-5 and YM-7), comparing with reference amoebae, A. culbertsoni, A. polyphaga and A. royreba. Trophozoites of six Acanthamoeba spp. had typical acanthopoda, but did not show any morphological differences. Cyst of Acanthamoeba sp. YM-7 was similar to that of A. polyphaga which was designated as group Ⅱ Acanthamoeba. Morphologically other amoebae belonged to group Ⅲ Acanthamoeba. Digestion of 18S-srDNA with six enzymes, Dde Ⅰ, Hae Ⅲ, Hind Ⅲ, EcoR Ⅰ, Rsa Ⅰ and Sph Ⅰ, resulted in various DNA fragments. Using the method of Nei and U (1979) for RFLP analysis, genetic divergence was not observed between A culbertsoni and Acanthamoeba sp. YM-4. Between A. culbertsoni and Acanthamoeba sp. YM-5, genetic distance was 0.070, 0.364 between Acanthamoeba sp. YM-4 and A. polyphaga, and 0.277 between Acanthamoeba sp. YM-7 and A. polyphaga. In comparison with A. culbertsoni and A. polyphaga, Acanthamoeba sp. YM-7 showed genetic distance of 0.330 and 0.154, respectively. Thus, Acanthamoeba sp. YM-7 is a similar species to A. polyphaga. Acanthamoeba sp. YM-5 is a different species from A. polyphaga, but closely-related to A. culbertsoni. Acanthamoeba sp. YM-4 can be classified as a subspecies or a strain of A. culbertsoni.

교육투자가 임금에 미치는 효과에 관한 실증분석

신경수,송일호 東國大學校 社會科學硏究院 2005 사회과학연구 Vol.12 No.1

The purpose of this study is to estimate the effect of educational investment on wage. According to the empirical results, the wage gaps for each education level are observed significantly, because the higher education level means to get more earnings. Moreover, the wage premium of middle school is almost vanished. This study also explores the marginal rate of returns of educational investment. First of all, the marginal rates of returns in high school and university have drastically declined since 2000. The marginal rates of returns in college graduates has drastically increased since 2000. Secondly, the marginal rate of returns of female is superior to that of male. Third, as the marginal rate of returns to education in middle school is decreased rapidly, that is almost vanished at present. In addition, this study estimates the marginal rate of returns to education by education level. The marginal rate of returns of university graduates is the highest, and that of high school graduates is better than that of college graduates. It also shows that the marginal rates of returns of college graduates exceeds that of university graduates. Finally, this study shows that the academic wage gap, that was declining after 1980's, has been increased since mid of 1990's. Second, the academic wage gap of female is higher than that of male.

원전의 가동중검사 관련 각국의 기술기준 비교고찰

신호상,김경조,장창희,강석철 한국비파괴검사학회 2004 한국비파괴검사학회지 Vol.24 No.2

각국의 법령에서는 원자력발전소에서 발생할 수 있는 사고로부터 국민의 건강과 재산을 보호하기 위해 원자력발전소의 압력경계 주요 용접부 등에 대해서는 주기적으로 열화의 정도를 파악하기 위한 가동중 비파괴검사를 수행하도록 규정하고 있으며, 이를 뒷받침하기 위한 가동중검사 기술기준을 제정하여 이행하고 있다. 최근 리스크를 반영한 가동중검사 프로그램을 개발하여 적용을 시도하고 있지만, 기존의 결정론적 검사대상 선정 프로그램은 아직도 상당한 의미가 있다고 할 수 있다. 본 논문에서는 가동중검사에 관한 선진국 즉, 미국, 프랑스, 캐나다, 일본 등의 기술기준과 IAEA에서 제시하는 안전지침에 비교검토를 통하여 현재 국내 가동중검사 기술기준의 개선방안을 모색하고자 하였다. In each country, the periodic ISI(In-Service Inspection) is required by the law to protect the public health and property from the potential accident of the nuclear facilities. To support the implementation of ISI program, the prescriptive ISI technical standards have been established. As the key parts of the ISI program, the non-destructive examination techniques are widely used to identify the degree of degradation of the pressure boundary components and welds. Recently, the risk informed-ISI program has been developed and implemented in several countries. Nonetheless, the existing ISI program which prescriptively decides the scope of inspection still has its own significance. In this article, the technical standards of ISI in leading countries like US, France, Canada, and Japan are reviewed and compared with the safety guide by IAEA. An outline to revise the domestic technical standards of ISI has been suggested.

피임제(Norinyl)가 흰쥐 난소의 Alkaline Phosphatase 활성에 미치는 영향

신경숙,이군자,정호삼,이규식 대한해부학회 1985 Anatomy & Cell Biology Vol.18 No.1

In mammals, female sexual hormones secrete at different rate during the sexual cycle so that the follicular cells and the granulosa cells undergo structural and functional changes. Therefore the authors undertook to pursue the changes of the graafian follicle during the estrous cycle and the effect of the oral-contraceptive. Female Sprague-Dawley rats weighing 150g were used as a experimental animal. Vaginal smears were obtained daily and only animals that showed consecutive four-day estrous cycle were used. The animals of the oral-contraceptive administered groups were administered norinyl, dissolved in distilled-water. The ovary was fixed with 10% neutral formalin and sliced at 14 ㎛ thickness in a frozen cryostat. The activity of alkaline phosphatase was observed by the Gomori's method for histochemical study. The other ovary was homogenized and the homogenate was assayed by the Lowry's method for biochemical study. The results were as follows. 1) In the follicle alkaline phosphatase activity was positive in the theca layer while the granulosa layer was negative. Strong activity were shown at proestrous and metestrous stage during the estrous cycle. And alkaline phosphatase activity of the ovary did not change markedly during the estrous cycle but the activity of estrous stage were slightly higher than other stages. 2) In the norinyl-administered group, the alkaline phosphatase activity of the ovary did not change during the estrous cycle. And the activity of the administered groups was lower than the control group.

인쇄매체를 이용한 보건교육자료의 가시성에 영향을 주는 요인에 관한 연구

신준호,정은경,이정애,오승진,김신월 全南大醫大 2001 전남의대학술지 Vol.37 No.2

대요근 근구 차단술의 해부학적 고찰 및 임상 효과

신경호,김헌,최락민,김희상,안경회 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.2