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현대건축의 형태표현에 나타난 '낯설게 하기(defamiliarization)'의 경향에 관한 연구
권유경,김성우,김영태 영남대학교 환경문제연구소 2000 環境硏究 Vol.19 No.2
본 연구는 1920년 러시아 문학의 현상으로 나타난 '낯설게 하기'의 이념을 현대건축의 조형표현 및 이론적 상관성으로 관련짓고자 한다. 현대건축에 있어서 중요한 이해와 경향인 '낯설게하기'를 통해 재발견되는 형식적인 표현으로써 현대건축에서 직접적 혹은 간접적으로 인용되고 있다. 내용보다 형식의 문제에 주목했던 러시아 형식주의의 '낯설게(defamiliarization)'을 통하여 현대건축에서 나타나고 있는 복잡성, 다양성, 혼성, 애매성 등을 제(諸)특성으로 보고, '낯설게 하기'의 목적과 수법들을 조형예술의 표현 특성 및 조형이념과 관련하여 건축적인 체계로 유형화하고 현대건축에서 나타나고 있는 경향 및 형태표현을 파악하는데 그 의의를 두고자 한다. This study related the idea of 'defamiliarization' in 1920's Russian Literature to the phenomenon of modern architecture and analyzed formative expression and theoretical correlativity. It is significant to understand the tendency of modern 'defamiliarization' which has comparatively been cited many times and expressed directly or indirectly in modern architecture. Expecially the 'defamiliarization' theory, with the experimental spirit of Russia constructivism, connected to formative art and can be related to the feature of complexity, variety, perplexity, conceptualization which are prominent in modern architecture. The meaning of 'defamiliarization' involves all the meanings of something new, creative, progressive and throughout this pursuing destruction of fixed idea, inhered newness, deautomatism in the process of consciousness usual environment, subconsciousness. This become the motive which formed important basis of making a new ideology in the formative art since Avant-garde and this logic offers theoretical basis correlated the Avant-garde spirit as a stream of breaking the existing convention.
Yu, Eun Jeong,Lee, Young,Rha, Sun Young,Kim, Tae Soo,Chung, Hyun Cheol,Oh, Bong Kyeong,Yang, Woo Ick,Noh, Sung Hoon,Jeung, Hei-Cheul American Association for Cancer Research 2008 Molecular Cancer Research Vol.6 No.10
<P>We investigated the biological role of thymidine phosphorylase (TP), an angiogenic factor, in gastric cancer cell migration and invasion and explored a therapeutic approach for high TP-expressing tumors using TP enzymatic inhibitor (TPI) and rapamycin. We established TP cDNA overexpressing gastric cancer cell lines (MKN-45/TP and YCC-3/TP) and did invasion and adhesion assays with Matrigel-coated transwell membranes. The related signal pathway using recombinant human TP (rhTP), deoxy-d-ribose (D-dRib), and signal pathway inhibitors (wortmannin, LY294002, and rapamycin) was investigated. First, AGS and MKN-1 gastric cancer cell lines showed dose-dependent up-regulation of invasiveness through Matrigel following treatment with rhTP or D-dRib. TP-overexpressing cancer cell lines displayed increased migration and invasion activity, which doubled with rhTP and D-dRib treatment. This activity depended on the enzymatic activity of TP, and TP stimulated the adhesion of cancer cells onto Matrigel and induced actin filament remodeling. Finally, we showed that this activity is related to increased phosphatidylinositol 3-kinase activity in TP-overexpressing cells and that combination treatment with rapamycin and TP enzymatic inhibitor produces an additive effect to abrogate TP-induced invasion. Taken together, TP increases the migration and invasion of gastric cancer cells, especially in TP-expressing cells. Therapies targeting TP might diminish the propensity for invasion and metastasis in gastric cancer.</P>
Kyeong-Rok Kang,Jae-Sung Kim,Jeong-Yeon Seo,HyangI Lim,Tae-Hyeon Kim,Sun-Kyoung Yu,Heung-Joong Kim,Chun Sung Kim,Hong Sung Chun,Joo-Cheol Park,Do Kyung Kim 대한생리학회-대한약리학회 2022 The Korean Journal of Physiology & Pharmacology Vol.26 No.1
The aim of the present study was to investigate the physiological role of nicotinamide phosphoribosyltransferase (NAMPT) associated with odontogenic differentiation during tooth development in mice. Mouse dental papilla cell-23 (MDPC- 23) cells cultured in differentiation media were stimulated with the specific NAMPT inhibitor, FK866, and Visfatin (NAMPT) for up to 10 days. The cells were evaluated after 0, 4, 7, and 10 days. Cell viability was measured using the 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide assay. The mineralization assay was performed by staining MDPC-23 cells with Alizarin Red S solution. After cultivation, MDPC-23 cells were harvested for quantitative PCR or Western blotting. Analysis of variance was performed using StatView 5.0 software (SAS Institute Inc., Cary, NC, USA). Statistical significance was set at p < 0.05. The expression of NAMPT increased during the differentiation of murine odontoblast-like MDPC-23 cells. Furthermore, the up-regulation of NAMPT promoted odontogenic differentiation and accelerated mineralization through an increase in representative odontoblastic biomarkers, such as dentin sialophosphoprotein, dentin matrix protein-1, and alkaline phosphatase in MDPC-23 cells. However, treatment of the cells with the NAMPT inhibitor, FK866, attenuated odontogenic differentiation, as evidenced by the suppression of odontoblastic biomarkers. These data indicate that NAMPT regulated odontoblastic differentiation through the regulation of odontoblastic biomarkers. The increase in NAMPT expression in odontoblasts was closely related to the formation of the extracellular matrix and dentin via the Runx signaling pathway. Therefore, these data suggest that NAMPT is a critical regulator of odontoblast differentiation during tooth development.
( Kyeong Min Kim ),( Seon A Jeong ),( Tae Hyun Ban ),( Yu Ah Hong ),( Seun Deuk Hwang ),( Sun Ryoung Choi ),( Hajeong Lee ),( Ji Hyun Kim ),( Su Hyun Kim ),( Tae Hee Kim ),( Ho-seok Koo ),( Chang-yun 대한신장학회 2024 Kidney Research and Clinical Practice Vol.43 No.1
Korean Renal Data System (KORDS) is a nationwide end-stage renal disease (ESRD) registry database operated by the Korean Society of Nephrology (KSN). Diabetes mellitus is currently the leading cause of ESRD in Korea; this article provides an update on the trends and characteristics of diabetic ESRD patients. The KORDS Committee of KSN collects data on dialysis centers and patients through an online registry program. Here, we analyzed the status and trends in characteristics of diabetic chronic kidney disease stage 5D (CKD 5D) patients using data from 2001 to 2021. In 2021, the dialysis adequacy of hemodialysis (HD) was lower in diabetic CKD 5D patients than in nondiabetic CKD 5D patients, while that of peritoneal dialysis (PD) was similar. Diabetic CKD 5D patients had a higher proportion of cardiac and vascular diseases and were more frequently admitted to hospitals than nondiabetic CKD 5D patients, and the leading cause of death was cardiac disease. From 2001 to 2020, diabetic CKD 5D patients had a higher mortality rate than nondiabetic CKD 5D patients, but in 2021 this trend was reversed. Diabetic PD patients had the highest mortality rate over 20 years. The mortality rate of diabetic HD patients was higher than that of nondiabetic HD patients until 2019 but became lower starting in 2020. There was a decreasing trend in mortality rate in diabetic CKD 5D patients, but cardiac and vascular diseases were still prevalent in diabetic CKD 5D patients with frequent admissions to hospitals. More specialized care is needed to improve the clinical outcomes of diabetic CKD 5D patients.
Hypermethylated promoters of tumor suppressor genes were identified in Crohn’s disease patients
( Tae-oh Kim ),( Yu Kyeong Han ),( Joo Mi Yi ) 대한장연구학회 2020 Intestinal Research Vol.18 No.3
Background/Aims: Overwhelming evidence suggests that inflammatory bowel disease (IBD) is caused by a complicated interplay between the multiple genes and abnormal epigenetic regulation in response to environmental factors. It is becoming apparent that epigenetic factors are significantly associated with the development of the disease. DNA methylation remains the most studied epigenetic modification, and hypermethylation of gene promoters is associated with gene silencing. Methods: DNA methylation alterations may contribute to the many complex diseases development by regulating the interplay between external and internal environmental factors and gene transcriptional expression. In this study, we used 15 tumor suppressor genes (TSGs), originally identified in colon cancer, to detect promoter methylation in patients with Crohn’s disease (CD). Methylation specific polymerase chain reaction and bisulfite sequencing analyses were performed to assess methylation level of TSGs in CD patients. Results: We found 6 TSGs (sFRP1, sFRP2, sFRP5, TFPI2, Sox17, and GATA4) are robustly hypermethylated in CD patient samples. Bisulfite sequencing analysis confirmed the methylation levels of the sFRP1, sFRP2, sFRP5, TFPI2, Sox17, and GATA4 promoters in the representative CD patient samples. Conclusions: In this study, the promoter hypermethylation of the TSGs observed indicates that CD exhibits specific DNA methylation signatures with potential clinical applications for the noninvasive diagnosis of IBD and the prognosis for patients with IBD. (Intest Res 2020;18:297-305)
Kyeong-Rok Kang,Jae-Sung Kim,Tae-Hyeon Kim,Jeong-Yeon Seo,Jong-Hyun Park,Jin Woong Lim,Sun-Kyoung Yu,Heung-Joong Kim,Sang Hun Shin,Bo-Ram Park,Chun Sung Kim,Do Kyung Kim 대한구강생물학회 2020 International Journal of Oral Biology Vol.45 No.3
Acacetin, which is present in damiana (Turnera diffusa ) and black locust (Robinia pseudoacacia ), has several pharmacologic activities such as antioxidant, anti-inflammatory, and anti-proliferative effects on cancer cells. However, the effect of acacetin on head and neck cancers has not been clearly established. This study aimed to examine the effects of acacetin on cell growth and apoptosis induction in FaDu human pharyngeal carcinoma cells. These were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, Live/Dead cell assay, 4′,6-diamidino-2-phenylindole dihydrochloride staining, caspase-3 and caspase-7 activation assay, and immunoblotting in FaDu cells. Acacetin induced FaDu cell death in a dose-dependent manner, with an estimated IC50 value of 41.9 µM, without affecting the viability of L-929 mouse fibroblasts as normal cells. Acacetin treatment resulted in nuclear condensation in the FaDu cells. It promoted the proteolytic cleavage of procaspase-3, -7, -8, and -9 with increasing amounts of the cleaved caspase isoforms in FaDu cells. Acacetin-induced apoptosis in FaDu cells was mediated by the expression of Fas and activation of caspase-8, caspase-3, and poly (ADP-ribose) polymerase. Immunoblotting showed downregulation of the anti-apoptotic mitochondrial proteins Bcl-2 and Bcl-xL, but upregulation of the mitochondria-dependent pro-apoptotic proteins Bax and Badin FaDu cells after acacetin treatment. These findings indicate that acacetin inhibits cell proliferation and induces apoptotic cell death in FaDu human pharyngeal carcinoma cells via both the death receptor-mediated extrinsic apoptotic pathway and the mitochondria-mediated intrinsic apoptotic pathway.