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Antimicrobial Agent from Schima wallichii ssp. liukiuensis against Candida spp.
Kuem Shin,Ji-Yun Min,Seung-Mi Kang,Dong-Jin Park,Hyun-Jin Song,Oh-Woong Kwon,Jae-Kyung Yang,Chandrakant S. Karigar,Myung-Suk Choi 한국약용작물학회 2009 한국약용작물학회지 Vol.17 No.1
This study carried out development of a natural antimicrobial agent from Schima wallichii ssp. liukiuensis. Compound I exhibiting potent antimicrobial activity against Candida spp. was isolated from the methanol extracts of Schima wallichii ssp. liukiuensis. The structure of I identified as a sterol glycoside consisted of a trisaccharide and α1-sitosterol. Trisaccharide composed of L-rhamnose, D-galactose and D-glucose residues. The antimicrobial activity of I was selective on yeast rather than bacteria or other fungi. Compound I was demonstrated to be ineffective against toxicity to mouse liver cells where as protective to human dermal fibroblast cells at low concentrations. Thus, it is reasonable to expect a sterol glycoside (I) as a valuable alternative for synthetic antifungal.
Kuem-Ju Lee,Dong-Hoon Kim,Song-Hyen Choi,You-Chan Shin,Sang-Ha Park,Bo-Hyun Moon,Seung Woo Kang,Eujin Cho,Sang-Hyun Choi,Boe-Gwun Chun,Min-Soo Lee,Kyung-Ho Shin 대한생리학회-대한약리학회 2007 The Korean Journal of Physiology & Pharmacology Vol.20 No.3
Recent studies suggest that alterations in glutamate receptor subunit levels in mesocorticolimbic dopamine areas could account for neural adaptations in response to psychostimulant drugs. Although many drugs of abuse induce changes in ionotropic glutamate receptor subunits in mesocorticolimbic dopamine areas, the changes of ionotropic glutamate receptor subunits by repeated nicotine treatment in these areas are not known. To answer this question, we injected male Sprague-Dawley rats twice daily with nicotine (0.4 mg/kg) or saline (1 ml/kg) for 10 days. The immunoreactivity of NR1, GluR1, and GluR2 glutamate receptor subunits was examined 16∼18 h after the last injection of saline or nicotine. Repeated nicotine treatment significantly increased NR1 levels in the ventral tegmental area (VTA). In addition, repeated nicotine treatment showed a tendency towards an increase in GluR1 levels in the VTA as well as in striatum. However, there was no significant change in glutamate receptor subunits in other areas including nucleus accumbens (NAc). These results demonstrate that repeated nicotine treatment increases NR1 levels in VTA similarly to other drugs of abuse, suggesting that elevated glutamate receptor subunits in the VTA, but not NAc may be involved in the excitation of mesocorticolimbic dopamine neurons by nicotine.
Lee, Kuem-Ju,Kim, Dong-Hoon,Choi, Song-Hyen,Shin, You-Chan,Park, Sang-Ha,Moon, Bo-Hyun,Kang, Seung-Woo,Cho, Eu-Jin,Choi, Sang-Hyun,Chun, Boe-Gwun,Lee, Min-Soo,Shin, Kyung-Ho The Korean Society of Pharmacology 2007 The Korean Journal of Physiology & Pharmacology Vol.11 No.4
Recent studies suggest that alterations in glutamate receptor subunit levels in mesocorticolimbic dopamine areas could account for neural adaptations in response to psychostimulant drugs. Although many drugs of abuse induce changes in ionotropic glutamate receptor subunits in mesocorticolimbic dopamine areas, the changes of ionotropic glutamate receptor subunits by repeated nicotine treatment in these areas are not known. To answer this question, we injected male Sprague-Dawley rats twice daily with nicotine (0.4 mg/kg) or saline (1 ml/kg) for 10 days. The immunoreactivity of NR1, GluR1, and GluR2 glutamate receptor subunits was examined $16{\sim}18 h$ after the last injection of saline or nicotine. Repeated nicotine treatment significantly increased NR1 levels in the ventral tegmental area (VTA). In addition, repeated nicotine treatment showed a tendency towards an increase in GluR1 levels in the VTA as well as in striatum. However, there was no significant change in glutamate receptor subunits in other areas including nucleus accumbens (NAc). These results demonstrate that repeated nicotine treatment increases NR1 levels in VTA similarly to other drugs of abuse, suggesting that elevated glutamate receptor subunits in the VTA, but not NAc may be involved in the excitation of mesocorticolimbic dopamine neurons by nicotine.
신경호,김성진,이금주,신승건,신유찬,이민수,Shin, Kyung-Ho,Kim, Sung Jin,Lee, Kuem Ju,Shin, Seung Gun,Shin, You Chan,Lee, Min-Soo The Korean Society of Biological Psychiatry 2003 생물정신의학 Vol.10 No.1
Corticotropin-releasing factor(CRF) and neuropeptide Y(NPY) are known to play important roles in mediating stress responses and stress-related behavior. To elucidate the role of neuropeptides in response to the condition that had paired with traumatic event, we observed the changes of CRF and NPY by immunohistochemistry using a conditioned footshock paradigm. Male Sprague-Dawley rats were placed in a shuttle box and exposed to 20 pairings of a tone(< 70dB, 5sec) followed by a footshock(FS, 0.8mA, 1sec) over 60min. A second group was exposed to the tone-footshock pairings, returned to the homecage for 2days, and then reexposed to the test chamber and 20tones alone for 60min, prior to sacrifice. Control groups were : a) sacrificed without exposure to FS ; b) exposed to the tone-footshock pairings and then sacrificed two days later ; or c) exposed to the chamber and tones alone, returned to the homecage for 2days and then reexposed to the chamber and 20tones over 60min prior to sacrifice. CRF was increased in animals exposed to FS or the aversive condition(context and tone) that had paired to FS in bed nucleus of the stria terminalis (BNST) compared to the control. NPY was increased by FS in amygdala and PVN, but the condition previously associated with FS results in slight increase only in amygdala area. These results suggest that the BNST appears to be the mostly involved neural circuit in response to explicit cues previously paired with footshock. Moreover, this study raise the possibility that increased CRF peptide in the BNST in response to re-exposure to the aversive condition may underlie, in part, the experience of conditioned fear-related anxiety behavior.
Kinetics of Cultivating Mammalian Cells in Fed-Batch Process for the Production of Erythropoeitin
Yu, Ho Kuem,Choi, Suk Kyu,Lee, Youn Soo,Shin, Kwang Soon,Hwang, Hee Koo,Lee, Hyeon Yong 한국산업미생물학회 1991 한국미생물·생명공학회지 Vol.19 No.5
유전자 재조합된 동물 세포의 유가 배양시 1.85×10 exp(-10)(mmole/cell/h)의 비 glucose 소비속도와 4.7×10 exp(-7)(㎍/cell/h)의 erythropoeitin(EPO)비 생산속도를 유지할 수 있었다. 또한 이같은 배양에서 회분 및 연속 배양에서보다 높은 세포수를 얻었으며 전 배양이 유사 안정상태에 도달하는 배양 후기에는 glutamolysis가 생육 공정에 매우 중요한 역할을 하고 있음이 확인됐다. 유가 배양시 13(mmole/l)의 glucose 농도에서 생육 제한 현상이 일어났으며, 이같은 농도에 도달할 때까지는 glucose의 농도가 증가함에 따라 배양시간의 경과와 함께 EPO 생산성이 증가했다. 1.85×10 exp(-10)(mmole/cell/h) of specific glucose consumption rate was obtained under fed-batch cultivation of recombinant mamalian cells with maintaining 4.7×10 exp(-7)(㎍/cell/h) of average specific erythropoeitin production rate. Higher maximum cell density was also achieved than for both cases of batch and perfusion cultivations. It proves that glutamolysis dominates metabolic pathways at latter period of cultivation where quasi steady state was maintained. Substrate limitation of glucose concentration was estimated as 13 (mmole/l) under fed-batch conditions, while specific product production rate was decreased according to cultivation time, erythropoeitin production was increased as glucose concentration in the media increased up to 13.2(mole/l).
류마토이드 관절염 환자에서 금치료후 발생한 미만성 간질성 폐질환 1 예
이재성,김미선,이신호,서기석,장희경,정만홍,조기범,최환준,장태원,정숙금 대한내과학회 1994 대한내과학회지 Vol.46 No.3
A 56-year-old woman with rheumatoid arthritis for 9 years was admitted in Oct. 1989 because of skin eruption, non-productive cough and exertional dyspnea for 1 month. She had been treated with gold sodium thiomalate (accumulated dose: 735 mg) for 3 months. Bibasilar inspiratory crackles were heard. Chest roentgenogram and computed tomogram showed diffuse interstitial infilterates. The lung specimen by open lung biopsy demonstrated alveolar septa1 thickening and interstitial lymphocyte infiltrates with fibrosis. The drug history, clinical features and the pathological findings support the diagnosis of gold pneumonitis.