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번식생리 및 내분비 : 제주흑우 동결정액 제조 시 Amide 계열의 동결보호제가 동결 융해 후 정자의 성상에 미치는 영향
고문석 ( Moon Suck Ko ),강태영 ( Tae Young Kang ),최선호 ( Sun Ho Choi ),조원모 ( Won Mo Cho ),오영미 ( Young Mi Oh ),고민희 ( Min Hee Ko ),조상래 ( Sang Rae Cho ),오신애 ( Shin Ae Oh ) 한국동물자원과학회 ( 구 한국축산학회 ) 2012 한국축산학회지 Vol.54 No.2
본 연구는 희소 한우인 제주흑우의 정액 동결을 위하여 동결보호제로서 glycerol과 amide 계열의 동결보호제인 5%의 DMA, DMF 그리고 7%의 MF를 이용하여 동결 융해 후 정자의 운동성, 생존율, 정자막 온전성 및 정자의 첨체 양상의 변화 조사를 위하여 수행하였다. 제주흑우의 정액을 동결 한 결과 glycerol과 DMF를 사용하였을 때 운동성은 64.00±9.62와 59.00±5.48로 DMA와 MF에 비해 유의적으로 높았으며(p<0.05), 생존율 역시 glycero과 DMF 처리구에서 58.25±6.63과 53.05±3.77로 유의적으로 높게 나타났다(p<0.05). 그러나 glycerol과 DMF 처리구 간의 유의적 차이는 나타나지 않았다. 정자막 온전성 검사(HOST)에 있어서도 역시 glycerol과 DMF 처리에서 정자 미부의 팽창 비율 역시 45.12±25.08과 44.95±8.58로 유의적으로 높았다(p<0.05). 정자의 첨체막 양상 변화에 있어 F pattern의 비율이 MF 처리시 다른 처리구에 비하여 유의적으로 낮았으며(p<0.05), B pattern의 비율은 DMA, DMF 및 MF의 사용시 glycerol에 비하여 유의적으로 증가하였다(p<0.05). AR pattern의 비율에 있어서는 DMF를 제외한 amide의 사용은 F pattern의 감소와 조기 첨체반응에 의한 유의적인 AR pattern의 증가를 볼 수 있었다(p<0.0.5). 그러나 DMF의 사용은 glycerol 보다 AR pattern의 비율이 유의적으로 낮았다(p<0.05). 동결 융해 후 시간에 따른 정자의 생존율과 정자 미부 팽창 비율에 있어서 융해 초기에는 glycerol에서 DMF 보다 높은 생존율과 정자 미부 팽창율을 나타냈으나, 1시간 이후부터는 DMF에서 glycerol 보다 높은 생존율과 정자 미부 팽창율을 나타내었다. 이러한 결과는 희소 가축의 생식세포 보존 및 유전자원 확립을 위한 중요한 자료가 될 것이며, 제주흑우 및 희소 가축의 안정적이고 효율적인 동결 정액 제조 연구에 있어 중요한 정보를 제공할 것으로 평가된다. The objective of this study was to examine the effect of amides as a cryoprotectant for semen cryopreservation in Korean Jeju Black Bull. The semen was cryopreserved with extenders containing 5% dimethyl acetamide(DMA), 5% dimethyl formamide (DMF), 5% methyl formamide(MF) or 7% glycerol. Post-thawed sperm were evaluated for sperm motility, viability, acrosome integrity and membrane integrity. Post-thawed sperm motility was significantly higher(p<0.05) in glycerol and DMF(64.00%± 9.62 and 59.00%±5.48, respectively) than DMA and MF(50.00%±3.24 and 44.00%±4.18, respectively). Sperm viability wassignificantly higher(p<0.05) in glycerol and DMF(58.25%±7.35 and 53.05%±3.77, respectively) than others. However, for sperm motility and viability, there were no differences among glycerol and DMF. Also, swelling sperm ratio by hypo-osmetic selling test(HOST) was significantly increased(p<0.05) in glycerol and DMF treatments(45.12%±25.08 and 44.95%±8.58, respectively). The percentage of capacitated sperm assessed by CTC staining, F pattern was lower(p<0.05) in DMF than others. B pattern was increased(p<0.05) in DMA, DMF and MF when compared with glycerol. AR pattern ratio was decreased(p<0.05) in glycerol and DMF when compared with DMA and MF. These results suggested that amides performed better and could be used as a cryoprotectant for semen freezing of Korean Jeju Black Bull.
( Jae Min Shin ),( Hyun Seok Choi ),( Seong Hun Moon ),( Hyun Woo Kim ),( Jeong Eun Kim ),( Joo Yeon Ko ),( Young Suck Ro ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: Moisturizers are a standard treatment and prevention strategy for atopic dermatitis (AD). Recently, interest in moisturizers containing anti-inflammatory and anti-microbial agents has been increasing. Objectives: To evaluate and compare the therapeutic and preventive effects of moisturizers containing licochalcone A or 1% hydrocortisone (HC) lotion in patients with mild to moderate AD. Methods: A prospective, randomized, investigator-blinded, 4 weeks study was conducted. Twice daily, for the first week, 75 patients applied an oil-in-water moisturizer on one side of the arm and HC lotion on the other side. The patients applied the water-in-oil moisturizer on both sides for a further 3 weeks. Clinical outcomes were based on the investigator``s global assessment, local scoring of the AD index, trans-epidermal water loss, skin capacitance, and Staphylococcus aureus colonization. The patients’ global assessments of satisfaction and tolerability were also evaluated. Results: 62 patients completed the protocol. In both arms, there were significant clinical improvements at after 1 week and after 4 weeks compared to baseline. There was no significant difference in patient satisfaction between the moisturizer and HC arms. Conclusion: Moisturizer containing licochalcone A has comparable effectiveness to that of 1 % HC lotion so that may be used for both early-phase treatment and maintenance therapy in mild to moderate AD.
P241 : A clinicopathologic review on 101 cases of morphea
( Jae Min Shin ),( Hyun Seok Choi ),( Seong Hun Moon ),( Jeong Ho Hong ),( Jeong Eun Kim ),( Young Suck Ro ),( Joo Yeon Ko ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: Morphea is an autoimmune condition characterized by excessive accumulation of collagen in the skin and underlying tissues. Its exact pathogenesis is poorly understood and only a few clinical or histopathological studies have been conducted in Asian patients. Objectives: The aim of this study was to identify the clinical and histopathological characteristics of morphea in Korean patients. Methods: We reviewed the medical records of 101 patients retrospectively. We analyazed demographic characteristics, number of lesions, disease subtype, and histopathological findings. The density of inflammatory cell infiltrate was classified as absent, mild, moderate, or dense. Results: Overall, circumscribed morphea was the most common clinical type, followed by linear, generalized, and mixed types. In the pediatric group, linear morphea was the most common subtype. Disease duration was positively correlated with increased thickness of the skin (p<0.001). Of 89 biopsy samples, inflammatory cell infiltration was absent in 50, mild in 26, and moderate in 13 patients. Disease duration was not correlated with the density of inflammatory cell infiltration (p=0.110). Conclusion: Our study provides the clinical characteristics of morphea in Korean patients and reveals histopathologic findings which associated with duration of disease. Exact photogenic features of morphea remain unexplained, and further research, on a larger sample, would be desirable.