Molecular Cloning of β - Galactosidas from Bacillus subtilis HP - 4

KIM,JEONG HO,JAE CHANG LEE,JEONG WON HUH,KI CHUL CHUNG,YOUNG JIN BAEK,YOUNG KYU LEE 한국미생물생명공학회 1991 Journal of Microbiology and Biotechnology Vol.1 No.4

Presurgical Naso-Alveolar Molding Appliance for Unilateral Cleft Lip and Palate

Baek, Seung-Hak,Yang, Won-Sik,Kim, Sukwha 대한치과교정학회 1998 대한치과교정학회지 Vol.28 No.6

본 연구의 목적은 1)편측성 순구개열 신생아 환자에 대한 술전 비치조 정형장치(PNAM)치료를 소개하고 2) 이 장치의 치조골 정형 효과를 평가하는 것이다. 서울대학교 병원 교정과에 내원하여 PNAM장치를 사용하여 치료받은 4명의 편측성 순구개열 호나자(남자 3명, 여자 1명, 평균 초진연령: 23.2일)을 대상으로 하였으며, 치조골에서 대분절과 소분절 파열부간의 평균거리는 8.27mm였고 치조골 정형치료의 평균기간은 9.7주였다. 초진시(Ti)와 성공적인 치조골 정형술이 이루어진 시기(T2)에 이들 환자의 인상을 채득하여 모형을 제작한 후, 사진촬영과 digital caliper를 사용하여 7개의 길이 항목과 5개의 각도항목을 계측하였다. SPSS win. ver 7.5프로그램을 사용하여 통계처리 하였고, 평균치의 차이를 비교하기 위하여 paired t-test를 사용하였다. 1. 신생아 치조골 후방부는 치조골 정형술을 시행하는 동안 안정된 구조물이었다. 2. 치조골 정형술에 의하여 대분절의 전방 성장이 억제되었다. 3. 치조골 정형술에 의한 파열부의 패쇄는 주로 대분절 전방부의 내측과 후방 굴곡 및 소분절 전체의 외측 굴곡에 의한 것이었다. The goals of this study were to present presurgical naso-alveolar molding(PNAM) appliance in unilaterla cleft lip and palate treatment and to evaluate the effects of PNAM appliance on alveolar molding. Samples were consisted of 4 unilateral cleft lip and palate infants(3 males and 1 female, mean age=23.2 days after birth) who were treated with PNAM appliances in Department of Orthodontics, Seoul National University Dental Hospital. Average alveolar cleft gap between the greater and lesser segment was 8.27 mm and average duration of alveolar molding treatment was 9.7 weeks. These patients' models were obtained at initial visit(T1) and after successful alveolar molding(T2). Seven linear and five angular variables were measured by using photometry and digital caliper. All statistical analyses were performed by SPSS win ver. 7.5 program. Paired t-test was used to compare the mean values. 1. The posterior part of alveolar segments are the stable structures during alveolar molding treatment period in infants. 2. Forward growth of th greater segment may be hindered by the action of alveolar molding. 3. The closure of cleft gap during alveolar molding were usually due to inward and backward bending of the anterior part of the greater segment and outward bending of the whole lesser segment.

Physiochemical Response of Native Grasses under Drought and Salinity Stresses as Sustainable Garden Plant Materials

Saeng Geul Baek,Jung Won Shin,Jeong Min Seo,Jeong Min Kim8) 한국화훼학회 2024 한국화훼학회 정기총회 및 학술발표회 자료집 Vol.32 No.1

자동착유시스템 설치농가의 설치 후 만족도에 관한 실태조사

기광석,김종형,정영훈,김윤호,박성재,김상범,이왕식,이현준,조원모,백광수,김현섭,권응기,김완영,여준모,Ki, Kwang-Seok,Kim, Jong-Hyeong,Jeong, Young-Hun,Kim, Yun-Ho,Park, Sung-Jai,Kim, Sang-Bum,Lee, Wang-Shik,Lee, Hyun-June,Cho, Won-Mo,Baek, Kwang- 한국축산환경학회 2011 축산시설환경학회지 Vol.17 No.1

본 시험은 국내에 도입되기 시작한 AMS에 대하여 설치 동기, 설치 후 우유생산량과우유품질, AMS 설치계기 및 설치 후 만족도, AMS 운영상 애로점 및 건의사항 등을 조사 분석하였다. 설치 계기가 무엇인가에 대한 응답비율은 노동력 부족 44%, 체험목장으로 전환 25%, 소들의 고능력화에 대응 19% 순이었으며 기타 6%였다. AMS 설치농가의 사육경력은 평균 24.9년, 평균 연령은 53.2세로 나타났으며 가족노동력만으로 경영하는 목장이 조사농가의 38%, 고용+자가노동력 형태의 목장이 63%로 나타났다. 목장의 산유능력은 두당 평균 유량은 1일 $30.9{\ell}$, 평균 유지율 3.9%, 세균수는 9.1천개/$m{\ell}$ 수준이었다. 후계자 육성에 대한 질문에 매우 그렇다라고 응답한 비율은 68%였으며, 보통 18%, 아니다와 매우 아니다라고 응답한 비율은 18%로 나타났다. 시스템 운영현황 및 애로사항을 파악하기 위하여 AMS의 프로그램 주운영자에 대한 질문에 본인 44%, 부인 6%, 자녀 또는 후계자사람이 44%, 기타 6%나 되었다. AMS 운영상 가장 어려운 점에 대한 조사결과, 기기 작동 및 복잡한 프로그램 운영이 각각 31%로 가장 많았다. 그 외 기계 에러 및 고장에 대한 애로사항이 25%이였다. 도태원인에 대하여 유방염 28%, 번식장애 20%, 발굽질환 19%, AMS에 부적합한 유두 배열 12%, 분만 후 대사성질병 7%, 노산 등 기타 14%였다. 시스템 설치 후 유지보수 계약 체결유무 및 비용에 대하여 유지보수 계약 56%, 유지 보수 계약 미체결 44%이었다. 유지보수 계약 평균 계약비용은 연간 658만원 정도였다. 시스템 설치 후 항목별 만족도에 대하여 5점 만점에 3.9점이었으며, 노동력 절감 3.7점, 업체의 A/S 3.6점, 산유량 증가 3.2점, 원유의 체세포 수 저감 2.8점이었다. 시스템 설치 시 고려사항 및 건의사항에 대하여 고가의 장비를 효율적으로 사용하기 위하여 젖소의 유방과 유두 배열이 적합하도록 젖소를 선발하는 것, 시스템을 운용할 수 있는 능력, 적합한 환경, 재정적 능력과 부지 확보, 지속적인 관찰 등이 권장되어야 할 것으로 나타났으며, 설치 업체에 대해서는 컨설팅이 체계화될 필요가 가장 높게 나타났으며 이어서 보다 저렴한 설치와 서비스의 체계화를 요구하는 결과를 보였다. The present survey was conducted to provide basic information on automatic milking system (AMS) in relation to purchase motive, milk yield and quality, customer satisfaction, difficulties of operation and customer suggestions, etc. Purchase motives of AMS were insufficient labor (44%), planning of dairy experience farm (25%), better performance of high yield cows (19%) and others (6%), respectively. Average cow performance after using AMS was 30.9l/d for milk yield, 3.9% for milk fat, 9,100/ml for bacterial counts. Sixty-eight percentage of respondents were very positive in response to AMS use for their successors but 18% were negative. The AMS operators were owner (44%), successor (44%), wife (6%) and company worker (6%), respectively. The most difficulty (31%) in using AMS was operating the system and complicated program manual. The rate of response to system error and breakdown was 25%. The reasons for culling cow after using AMS were mastitis (28%), reproduction failure (19%), incorrect teat placement (12%), metabolic disease (7%) and others (14%), respectively. Fifty-six percentages of the respondents made AMS maintenance contract and 44% did not. Average annual cost of the maintenance contract was 6,580,000 won. Average score for AMS satisfaction measurement (1 to 5 range) was 3.2 with decrease of labor cost 3.7, company A/S 3.6, increase of milk yield 3.2 and decrease of somatic cell count 2.8, respectively. Suggestions for the higher efficiency in using AMS were selecting cows with correct udder shape and teat placement, proper environment, capital and land, and attitude for continuous observation. Systematic consulting was highly required for AMS companies followed by low cost for AMS setup and systematization of A/S.

A prospective association between dietary mushroom intake and the risk of type 2 diabetes: the Korean Genome and Epidemiology Study–Cardiovascular Disease Association Study

Yu-Mi Kim(Yu-Mi Kim),Hye Won Woo(Hye Won Woo),Min-Ho Shin(Min-Ho Shin),Sang Baek Koh(Sang Baek Koh),Hyeon Chang Kim(Hyeon Chang Kim),Mi Kyung Kim(Mi Kyung Kim) 한국역학회 2024 Epidemiology and Health Vol.46 No.-

Fabrication of an initially-focal-conic cholesteric liquid crystal cell without polymer stabilization

Baek, Jong-Min,Oh, Seung-Won,Kim, Sang-Hyeok,Yoon, Tae-Hoon Elsevier 2018 Displays Vol.52 No.-

<P><B>Abstract</B></P> <P>A dye-doped cholesteric liquid crystal (LC) cell with the focal-conic initial state is demonstrated. Although dye-doped LC/polymer composites have been widely used for LC light shutters, dye-doped LC cells with polymer structures suffer from the degradation of dichroic dyes during the UV curing process. To avoid this problem, we propose a dye-doped cholesteric LC cell using vertical alignment layers instead of polymer structures. We have shown that the focal-conic initial state can be obtained by homeotropic anchoring without the polymer structure. The proposed device not only provides the black color but can also hide objects behind a display panel in the focal-conic initial state without the degradation of dichroic dyes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We propose a dye-doped cholesteric LC cell using vertical alignment layers. </LI> <LI> The proposed device provides black color and hides objects behind a display panel. </LI> <LI> The focal-conic initial state can be obtained without the degradation of dyes. </LI> </UL> </P>

Prevalence and comorbidity of allergic diseases in preschool children

Kim, Hyeong Yun,Kwon, Eun Byul,Baek, Ji Hyeon,Shin, Youn Ho,Yum, Hye Yung,Jee, Hye Mi,Yoon, Jung Won,Han, Man Yong The Korean Pediatric Society 2013 Clinical and Experimental Pediatrics (CEP) Vol.56 No.8

Purpose: Allergic disease and its comorbidities significantly influence the quality of life. Although the comorbidities of allergic diseases are well described in adult populations, little is known about them in preschool children. In the present study, we aimed to assess the prevalence and comorbidity of allergic diseases in Korean preschool children. Methods: We conducted a cross-sectional study comprising 615 Korean children (age, 3 to 6 years). Symptoms of allergic diseases were assessed using the Korean version of the International Study of Asthma and Allergies in Childhood (ISAAC) questionnaire that was modified for preschool children. Comorbidities of allergic diseases were assessed by 'In the last 12 months, has your child had symptoms?'. Results: The prevalence of symptoms of asthma, allergic rhinitis, and atopic dermatitis as recorded using the ISAAC questionnaire, within the last 12 months was 13.8%, 40.7%, and 20.8%, respectively. The symptom rates of allergic conjunctivitis, food allergy, and drug allergy were 14.8%, 10.4%, and 0.8%, respectively. The prevalence of allergic rhinitis in children with asthma was 64.3% and that of asthma in children with allergic rhinitis was 21.6%. The prevalence of rhinitis in children with conjunctivitis was 64.8% and that of conjunctivitis in children with rhinitis was 23.6%. Conclusion: The prevalence of current rhinitis in our preschool children is shown to be higher than that previously reported. Allergic conjunctivitis is closely associated with asthma and allergic rhinitis. However, further studies are warranted to determine the prevalence and effects of these comorbidities on health outcomes in preschool children.

Smart SERS Hot Spots: Single Molecules Can Be Positioned in a Plasmonic Nanojunction Using Host-Guest Chemistry

Kim, Nam Hoon,Hwang, Wooseup,Baek, Kangkyun,Rohman, Md. Rumum,Kim, Jeehong,Kim, Hyun Woo,Mun, Jungho,Lee, So Young,Yun, Gyeongwon,Murray, James,Ha, Ji Won,Rho, Junsuk,Moskovits, Martin,Kim, Kimoon American Chemical Society 2018 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.140 No.13

<P>Single-molecule surface-enhanced Raman spectroscopy (SERS) offers new opportunities for exploring the complex chemical and biological processes that cannot be easily probed using ensemble techniques. However, the ability to place the single molecule of interest reliably within a hot spot, to enable its analysis at the single-molecule level, remains challenging. Here we describe a novel strategy for locating and securing a single target analyte in a SERS hot spot at a plasmonic nanojunction. The “smart” hot spot was generated by employing a thiol-functionalized cucurbit[6]uril (CB[6]) as a molecular spacer linking a silver nanoparticle to a metal substrate. This approach also permits one to study molecules chemically reluctant to enter the hot spot, by conjugating them to a moiety, such as spermine, that has a high affinity for CB[6]. The hot spot can accommodate at most a few, and often only a single, analyte molecule. Bianalyte experiments revealed that one can reproducibly treat the SERS substrate such that 96% of the hot spots contain a single analyte molecule. Furthermore, by utilizing a series of molecules each consisting of spermine bound to perylene bisimide, a bright SERS molecule, with polymethylene linkers of varying lengths, the SERS intensity as a function of distance from the center of the hot spot could be measured. The SERS enhancement was found to decrease as 1 over the square of the distance from the center of the hot spot, and the single-molecule SERS cross sections were found to increase with AgNP diameter.</P> [FIG OMISSION]</BR>

A Randomized Phase 2 Trial of Consolidation Chemotherapy After Preoperative Chemoradiation Therapy Versus Chemoradiation Therapy Alone for Locally Advanced Rectal Cancer: KCSG CO 14-03

Kim, Sun Young,Joo, Jungnam,Kim, Tae Won,Hong, Yong Sang,Kim, Jeong Eun,Hwang, In Gyu,Kim, Beom Gyu,Lee, Keun-Wook,Kim, Ji-Won,Oh, Ho-Suk,Ahn, Joong Bae,Zang, Dae Young,Kim, Dae Yong,Oh, Jae Hwan,Baek Elsevier 2018 International journal of radiation oncology, biolo Vol.101 No.4

<P><B>Purpose</B></P> <P>Preoperative chemoradiation therapy (CRT) followed by total mesorectal excision (TME) in locally advanced rectal cancer is the standard of care. To date, the role of consolidation chemotherapy after CRT has rarely been addressed through randomized trials. This study aimed to evaluate the efficacy of CRT followed by consolidation chemotherapy compared with CRT alone.</P> <P><B>Methods and Materials</B></P> <P>This study enrolled patients with adenocarcinoma of the rectum and cT3 or cT4 disease with any N category and no metastasis. In arm A (control arm), we planned CRT (50.4 Gy in 28 fractions) with capecitabine followed by TME. In arm B, 2 cycles of capecitabine and oxaliplatin were administered 1 week after the completion of CRT before TME (capecitabine, 1700 mg/m<SUP>2</SUP> per day from day 1 to 14, and oxaliplatin, 100 mg/m<SUP>2</SUP> on day 1, every 3 weeks). The downstaging rate (the proportion of ypT0 to ypT2 and ypN0M0) was the primary endpoint, which was to be tested with a 1-sided type I error of 15% and with 85% power.</P> <P><B>Results</B></P> <P>From September 2014 to February 2016, 110 patients (56 in arm A and 54 in arm B) were randomized and 108 (55 in arm A and 53 in arm B) started CRT. TME was conducted per protocol in 96 patients (52 in arm A and 44 in arm B). In arms A and B, downstaging was achieved in 21.2% and 36.4% (<I>P</I> = .077), respectively, and the pathologic complete response rate was 5.8% and 13.6% (<I>P</I> = .167), respectively. Grade ≥3 adverse events occurred in 3.6% of patients in arm A and 9.4% of patients in arm B during the preoperative treatment phase and in 1.9% and 9.0%, respectively, during the postoperative recovery phase.</P> <P><B>Conclusions</B></P> <P>Consolidation chemotherapy with 2 cycles of capecitabine and oxaliplatin demonstrated a marginal improvement in the downstaging rate. However, a phase 3 trial of this strategy is discouraged because of the high dropout rate and safety issues.</P>

Feasibility of Bilateral Crossing C7 Intralaminar Screws : A Cadaveric Study

Baek, Tae-Hyun,Kim, Ilsup,Hong, Jae-Taek,Kim, Daniel H.,Shin, Dongsuk,Lee, Sang-Won The Korean Neurosurgical Society 2014 Journal of Korean neurosurgical society Vol.56 No.1

Objective : When the pedicle screw insertion technique is failed or not applicable, C7 intralaminar screw insertion method has been used as an alternative or salvage fixation method recently. However, profound understanding of anatomy is required for safe application of the bilaterally crossing laminar screw at C7 in clinic. In this cadaveric study, we evaluated the anatomic feasibility of the bilateral crossing intralaminar screw insertion and especially focused on determination of proper screw entry point. Methods : The C7 vertebrae from 18 adult specimens were studied. Morphometric measurements of the mid-laminar height, the minimum laminar thickness, the maximal screw length, and spino-laminar angle were performed and cross-sectioned vertically at the screw entry point (spino-laminar junction). The sectioned surface was equally divided into 3 parts and maximal thickness and surface area of the parts were measured. All measurements were obtained bilaterally. Results : The mean mid-laminar height was 13.7 mm, mean minimal laminar thickness was 6.6 mm, mean maximal screw length was 24.6 mm, and mean spinolaminar angle was $50.8{\pm}4.7^{\circ}$. Based on the measured laminar thickness, the feasibility of 3.5 mm diameter intralaminar screw application was 83.3% (30 sides laminae out of total 36) when assuming a tolerance of 1 mm on each side. Cross-sectional measurement results showed that the mean maximal thickness of upper, middle, and lower thirds was 5.0 mm, 7.5 mm, and 7.3 mm, respectively, and mean surface area for each part was $21.2mm^2$, $46.8mm^2$, and $34.7mm^2$, respectively. Fourteen (38.9%) sides of laminae would be feasible for 3.5 mm intralaminar screw insertion when upper thirds of C7 spino-laminar junction is the screw entry point. In case of middle and lower thirds of C7 spino-laminar junction, 32 (88.9%) and 28 (77.8%) sides of laminae were feasible for 3.5 mm screw insertion, respectively. Conclusion : The vertical cross-sectioned area of middle thirds at C7 spinolaminar junction was the largest area and 3.5 mm screw can be accommodated with 77.8 % of feasibility when lower thirds were the screw entry point. Thus, selection of middle and lower thirds for each side of screw entry point in spino-laminar junction would be the safest way to place bilateral crossing laminar screw within the entire lamina. This anatomic study result will help surgeons to place the screw safely and accurately.