Development of a natural plant-nutrient from wasted tea leaves and stems

Kim , Jong Cheol,Kim , Rumi,Cho, Kyoung Hwan,Kim Yong Duck,Hwang, Jung Gyu,Han, Jae Yoon,Lee, Jong Gug 한국유기농업학회 2011 韓國有機農業學會誌 Vol.19 No.S

Plant biomass is a huge carbon-complex that has potential as a nutrient. Therefore we extracted and separated useful materials for plant growth from tea leaf and stem. The pre-treatment process including high temperature (200 OC) and pressure (20-40 kgf/cm2) was treated for several minutes and extracted at 120 ℃ for 30-60 minutes. After that the chemical compositions and ingredients were analyzed from that plantnutrient. As a result of mineral contents, calcium and magnesium concentrations are higher than other minerals. Also the result of carbohydrates analyses has shown that the sugar oligomer consists of xylose(95.3%) and glucose(4.7%), and the sugar monomer consists in the order of xylose (52.7%) > manose (22.8%) > arabinose (10.8%) > galactose (10.2%) > glucose (3.5%). Before applied to field, in vitro plant growth system and formulation were examined. To evaluate the effect of the nutrients, both strawberry green-house and persimmon fields were used in this test. The treated persimmons were heavier than controls scored at 13-22%. In addition, the storageperiod was extended in the treated strawberries. Interestingly in the treated strawberry, the contents of polyphenols were increased (38-57%). These results suggest that the plant-nutrient can afford to help for plant growth and storage, and it can be substituted for other commercial nutrients. In conclusion, this plant-nutrient may help to extend eco-friendly or organic farming in Hadong-gun area.

Predictive Model of Microcystins during the Destruction of Harvested Microalgal Cell by Cold Plasma

Rumi Park,Jong-Guk Kim,Hyun-Woo Kim 대한환경공학회 2019 대한환경공학회 학술발표논문집 Vol.2019 No.-

Removal Kinetics of Microcystins and Algal Biomass by Non- Thermal Plasma Oxidation

( Rumi Park ),( Hyunmin An ),( Jong-guk Kim ),( Hyun-woo Kim ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 ISSE 초록집 Vol.2019 No.-

Cyanobacterial toxin, microcystins, associated with microalgal blooms may cause risk to the ecosystem including livestock and human. In this study, the removal efficiency of microcystins and biomass was evaluated using non-thermal plasma technology, one of the advanced oxidation processes. The most frequently detected microcystin-LR, -RR and -YR were determined as indicators of the major algal toxins. Under the defined experimental conditions, organics as chemical oxygen demand was removed by 26.7% for 24 hours and biomass removal as volatile suspended solids was as high as 32.1%. During the reaction, the result of scanning electron microscope evidences that the non-thermal plasma can destroy microalgal cell walls effectively and make the microcystin inside the cell released to the outside of the cell. The determination results demonstrate the increase of microcystin-LR and -YR though microcystin-RR was decreased by 34.2%. The total microcystins were removed by 30.9% during the operation. We newly suggested a model equation considering the microcystin release during the cell destruction. The microcystin degradation rate and release rate were estimated to be 8.604 d^-1 and 0.369 d^-1, respectively, as a result of regression analysis. This study may contribute to predict the variability of microcystin removal depending on the existence of algae cells. A comparison with other studies further supports the applicability of the non-thermal plasma technology for the effective control of algal bloom and its toxicity.

저온플라즈마 산화공법을 이용한 미세조류 바이오매스 내 마이크로시스틴-LR, -RR, -YR의 제거 동역학

박루미 ( Rumi Park ),김종국 ( Jong-guk Kim ),김현우 ( Hyun-woo Kim ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 한국폐기물자원순환학회 추계학술발표논문집 Vol.2019 No.-

농업용수, 낙시터 등 다양한 용도로 이용되고 있는 국내 많은 저수지는 매년 부영양화에 따른 심각한 녹조문제를 겪고 있다(김승 2017). 저수지에 발생하는 녹조를 제어하기 위해 응집제 살포 및 준설, 인공식물섬 등의 물리화학적 방법이 연구되고 있으나 저수지에 발생한 녹조를 이루는 몇몇 남조류는 대표적으로 마이크로시스틴을 포함한 유해 조류독소를 형성하기 때문에 보다 효과적이며 확실한 처리기술이 필요하다. 본 연구는 녹조와 녹조 내 존재하는 대표적인 조류독소인 마이크로시스틴을 제어하기 위해 고급산화공법 중 한 가지로, 다양한 산화종을 생산하여 난분해성 화합물 및 고부하 유기물의 높은 처리성능이 입증된 저온플라즈마 산화공법을 적용하였다. 연구진은 저수지의 녹조를 수거, 농축한 미세조류 바이오매스를 대상으로 저온플라즈마 처리 시 마이크로시스틴-LR, -RR, -YR 3 종의 농도 변화를 관찰하였다. 반응조는 저온플라즈마 발생기에서 방전된 carrier gas가 기체 형태로 반응조 내부로 유입되는 형식으로 운전되었다. 시간에 따른 마이크로시스틴의 농도와 바이오매스 농도의 상관관계 및 동역학적 고찰은 회분식 실험 데이터를 분석하여 진행되었다. 채취한 시료는 1.2 μm GF/C 여과지로 미세조류 세포를 걸러 아세트산 용출에 투입되었다. 이렇게 용출된 세포 내 마이크로시스틴과 여과지로 거른 세포 외 용액의 마이크로시스틴 농도를 각각 분석하여 저온플라즈마 산화공법이 녹조의 바이오매스 농도와 마이크로시스틴에 미치는 영향을 고찰하였다. 본 연구는 매년 심각한 문제를 일으키는 저수지 내 녹조를 수거, 농축한 미세조류 바이오매스에 존재하는 유해 독성 성분을 저온플라즈마 산화공법을 통해 제거하여 고농도 바이오매스의 자원화 활용도를 높인다.

Circular Polyethylene Drape in Prevention of Surgical Site Infection (Cover Trial): Interim Analysis (Nct 03170843)

Ri Na YOO,HyungJin KIM,JaeIm LEE,Won-Kyung KANG,Chang Woo KIM,Sung Uk BAE,Byung Mo KANG,Joong Ho LEE,Rumi SHIN,Byung Kwan PARK,Heung Kwon OH,Han Deok KWAK,Woo Ram KIM,Bong Hyeon KYE 대한외과학회 2019 대한외과학회 학술대회 초록집 Vol.2019 No.10

Identification of a Polymyxin Synthetase Gene Cluster of Paenibacillus polymyxa and Heterologous Expression of the Gene in Bacillus subtilis

Choi, Soo-Keun,Park, Soo-Young,Kim, Rumi,Kim, Seong-Bin,Lee, Choong-Hwan,Kim, Jihyun F.,Park, Seung-Hwan American Society for Microbiology 2009 Journal of Bacteriology Vol.191 No.10

<B>ABSTRACT</B><P>Polymyxin, a long-known peptide antibiotic, has recently been reintroduced in clinical practice because it is sometimes the only available antibiotic for the treatment of multidrug-resistant gram-negative pathogenic bacteria. Lack of information on the biosynthetic genes of polymyxin, however, has limited the study of structure-function relationships and the development of improved polymyxins. During whole genome sequencing of <I>Paenibacillus polymyxa</I> E681, a plant growth-promoting rhizobacterium, we identified a gene cluster encoding polymyxin synthetase. Here, we report the complete sequence of the gene cluster and its function in polymyxin biosynthesis. The gene cluster spanning the 40.6-kb region consists of five open reading frames, designated <I>pmxA</I>, <I>pmxB</I>, <I>pmxC</I>, <I>pmxD</I>, and <I>pmxE</I>. The <I>pmxC</I> and <I>pmxD</I> genes are similar to genes that encode transport proteins, while <I>pmxA</I>, <I>pmxB</I>, and <I>pmxE</I> encode polymyxin synthetases. The insertional disruption of <I>pmxE</I> led to a loss of the ability to produce polymyxin. Introduction of the <I>pmx</I> gene cluster into the <I>amyE</I> locus of the <I>Bacillus subtilis</I> chromosome resulted in the production of polymyxin in the presence of extracellularly added l-2,4-diaminobutyric acid. Taken together, our findings demonstrate that the <I>pmx</I> gene cluster is responsible for polymyxin biosynthesis.</P>

Surgical Management of Sigmoid Volvulus: A Multicenter Observational Study

Keunchul Lee,오흥권,Jung Rae Cho,Minhyun Kim,Duck-Woo Kim,Sung-Bum Kang,Hyung-Jin Kim,Hyoung-Chul Park,Rumi Shin,Seung Chul Heo,Seung-Bum Ryoo,Kyu Joo Park,Seoul Colorectal Research Group 대한대장항문학회 2020 Annals of Coloproctolgy Vol.36 No.6

Purpose: This study aimed to evaluate real-world clinical outcomes from surgically treated patients for sigmoid volvulus.Methods: Five tertiary centers participated in this retrospective study with data collected from October 2003 through September 2018, including demographic information, preoperative clinical data, and information on laparoscopic/open and elective/emergency procedures. Outcome measurements included operation time, postoperative hospitalization, and postoperative morbidity.Results: Among 74 patients, sigmoidectomy was the most common procedure (n = 46), followed by Hartmann’s procedure (n = 23), and subtotal colectomy (n = 5). Emergency surgery was performed in 35 cases (47.3%). Of the 35 emergency patients, 34 cases (97.1%) underwent open surgery, and a stoma was established for 26 patients (74.3%). Elective surgery was performed in 39 cases (52.7%), including 21 open procedures (53.8%), and 18 laparoscopic surgeries (46.2%). Median laparoscopic operation time was 180 minutes, while median open surgery time was 130 minutes (P < 0.001). Median postoperative hospitalization was 11 days for laparoscopy and 12 days for open surgery. There were 20 postoperative complications (27.0%), and all were resolved with conservative management. Emergency surgery cases had a higher complication rate than elective surgery cases (40.0% vs. 15.4%, P = 0.034).Conclusion: Relative to elective surgery, emergency surgery had a higher rate of postoperative complications, open surgery, and stoma formation. As such, elective laparoscopic surgery after successful sigmoidoscopic decompression may be the optimal clinical option.

[OP-09] Antioxidant, Tyrosinase Inhibitory, and Acetylcholine esterase Inhibition Activities of Green Tea, Green Tea Seed, and Green Tea Pericarp

Young-Hong Jo,Jong-Cheol Kim,Rumi Kim,Jong-Hwa Lee,Suk-Ju Kim,Seung-Cheol Lee 한국식품영양과학회 2010 한국식품영양과학회 학술대회발표집 Vol.2010 No.10

Oncologic outcomes of preoperative stent insertion first versus immediate surgery for obstructing left-sided colorectal cancer

Kang, Sung Il,Oh, Heung-Kwon,Yoo, Jae Suk,Ahn, Soyeon,Kim, Min Hyun,Kim, Myung Jo,Son, Il Tae,Kim, Duck-Woo,Kang, Sung-Bum,Park, Young Soo,Yoon, Chang Jin,Shin, Rumi,Heo, Seung Chul,Lee, In Taek,Youk, Elsevier 2018 Surgical oncology Vol.27 No.2

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Colonic self-expanding metallic stenting (SEMS) is widely used for the treatment of malignant colonic obstruction as a bridge to elective surgery. However, the effects of colonic stenting on long-term oncologic outcomes are debatable. This study aimed to compare the long-term oncologic outcomes of preoperative SEMS insertion with those of immediate surgery in patients with obstructing left-sided colorectal cancer.</P> <P><B>Methods</B></P> <P>A cohort of consecutive patients who underwent radical surgery for obstructing left-sided colorectal cancer between 2004 and 2011 in five tertiary referral hospitals were analyzed. Long-term survivals were analyzed and adjusted using the inverse probability of treatment weighting method, based on propensity scores, to reduce selection bias.</P> <P><B>Results</B></P> <P>One hundred and nine patients underwent immediate surgery, and 226 underwent stent insertion before surgery. Disease-free survival did not differ significantly in both the unadjusted population (hazard ratio [HR] 1.063, 95% confidence interval [CI] 0.730–1.548; Log-rank, p = 0.746) and the adjusted population (HR 0.122, 95% CI 0.920–1.987; Log-rank, p = 0.122). Overall survival also did not differ significantly in both the unadjusted population (HR 0.871, 95% CI 0.568–1.334; Log-rank, p = 0.526) and the adjusted population (HR 1.023, 95% CI 0.665–1.572; Log-rank, p = 0.916). Defunctioning stoma formation was less in the SEMS insertion group than immediate surgery group (adjusted, 14.6% vs. 41.3%, p < 0.001).</P> <P><B>Conclusion</B></P> <P>The ‘bridge to surgery’ strategy using metallic stents was oncologically comparable to immediate surgery in patients with malignant left-sided colorectal obstruction.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Disease-free survival rate was comparable, SEMS insertion vs. immediate surgery. </LI> <LI> Overall survival rate was comparable between the two groups. </LI> <LI> Defunctioning stoma formation was less in the SEMS insertion group. </LI> <LI> The ‘bridge to surgery’ strategy using stent insertion was oncologically acceptable. </LI> </UL> </P>

STS-RFLP법을 이용한 국내지역 재배녹차의 비교분석

Kiu-Hyung Cho(조규형),Aruna Jo(조아르나),Tomohiko Tsuge,Jong Cheol Kim(김종철),Rumi Kim(김루미),Ho-Sung Yoon(윤호성),Gyung-Tae Kim(김경태) 한국생명과학회 2010 생명과학회지 Vol.20 No.9

최근 웰빙 열풍으로 나날이 녹차에 대한 관심과 소비가 증가하고, 생산 재배되고 있는 산지에 대한 브랜드화가 진행되고 있다. 하지만 지역에서 재배되고 있는 녹차품종의 구별 및 차이에 대해서는 아직 많은 연구가 되어 있지 않고 있다. 이 연구에서 국내 대표 녹차산지인 하동지역과 보성지역에서 채집한 녹차와 중국과 일본의 대표적 녹차품종을 가지고 STS-RFLP분석을 수행하였다. 페닐프로파노이드 생합성 경로에 관여하는 페닐알라닌 암모니아 리아제와 찰콘 합성효소 그리고 디하이드로플라보놀 4-리덕타아제 유전자의 암호영역과 비암호영역을 사용하여 이들 품종들을 구별할 수 있는 연구에 성공하였다. 이 논문에서는 녹차품종 구별에 사용 가능한 STS-RFLP법과 프라이머를 나타내었고, 하동지역과 보성지역의 녹차품종을 CHS 유전자의 CAPS 마커만으로 구별할 수 있는 방법을 찾아내어, 국내 지역간 품종의 구분 및 검증에 사용할 수 있다는 사실을 제시하였다. Consumption of green tea has increased along with increasing concern regarding healthier lifestyles, and many brands of green tea are sold with a label indicating the region of Korea in which the tea was produced. However, there is little information on identifying the difference between the green tea cultivars according to the region they were grown. Here, 9 green tea cultivars collected from Hadong region, Bosung region, China and Japan were subjected to the STS-RFLP analysis. Using the coding and noncoding DNA regions of genes related to the phenylpropanoid pathway, such as phenylalanine ammonia-lyase, chalcone synthase and dihydroflavonol 4-reductase, we have identified the differences between green tea cultivars according to the region they were grown in. In this study, we showed a STS-RFLP method of green tea analysis which easily distinguished different kinds of tea using the primers as described. In addition, we identified that the green tea cultivars from Hadong and Bosung displayed a different profile when PAL intron was digested with Dde I, suggesting that a rapid authentication system for green tea cultivars grown in different regions in Korea is available.