자궁내막증에 의한 골반 통증을 호소하는 불임환자에서 골반경하 자궁천골인대 소작술 ( laparoscopic uterosacral nerve ablation ) 의 임상적 효용성

문명진(Myoung Jin Moon),김대곤(Dae Kon Kim),윤경호(Kyung Ho Yoon),홍준식(Jun Sig Hong),김연주(Yon Ju Kim),김혜옥(Hye Ok Kim),양광문(Kwang Moon Yang),최규홍(Kyu Hong Choi),유근재(Keun Jai Yoo) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.12

N/A Objective : The aim of this study was to evaluate the efficacy of laparoscopic uterosacral nerve ablation (LUNA) in infertile women with chronic pelvic pain and endometriosis. Method : Prospective randomized study was performed in 20 infertile women with endometriosis and chronic pelvic pain, who had undergone of laparoscopic uterosacral nerve ablation at Samsung Cheil hospital between April and September, 1998. Statistical analysis was perfomed using by DBSTAT (ver. 2.0). Result : The pelvic pain score of pre-operation was 3.72±1.07 (mean±SD) and it was significantly decreased to 1.83±0.85 at 1 month later and 1.93±0.92, 1.69±0.60, 1.56±0.62, 1.56±0.81 each in second, third, fourth and fifth month after operation. There was no correlation between pelvic pain and stage of endometriosis. Pregnancy rate was 65% after LUNA (twelve deliveries, one pregnancy is ongoing at IUP 24 weeks). Conclusion : Laparoscopic uterosacral nerve ablation would be effective for infertile women with endometriosis and pelvic pain.

조산 예측을 위한 IGFBP-1 (Insulin-like Growth Factor-binding Protein-1)의 유용성에 대한 연구

최준식 ( Choe Jun Sig ),양재혁 ( Yang Jae Hyeog ),류현미 ( Ryu Hyun Mee ),김문영 ( Kim Mun Yeong ),한정렬 ( Han Jeong Lyeol ),안현경 ( Ahn Hyun Kyung ),신중식 ( Sin Jung Sig ),김주오 ( Kim Ju O ),문명진 ( Moon Myoung Jin ),정진훈 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.7

목적 : 조기 자궁 진통은 제태 기간 37주 이전에 자궁 수축이 일어나는 것을 의미하며, 조산의 대부분의 원인이다. 본 연구의 목적은 조기 양막 파수가 없는 산모에서 자궁 경부의 IGFBP-1 (phosphorylated isoform)을 측정하여, phIGFBP-1의 수치 증가와 조산과의 연관성을 보고자 하였다. 연구 방법 : 환자는 2002년 4월부터 2002년 12월까지 삼성제일병원 산부인과에서 조기 자궁 진통으로 진단 받은 57명의 산모를 대상으로 하였으며, 대조군은 환자군과 제태 기간이 유사한 무증상의 산모 30명을 대상으로 하였다. 소독된 경 (speculum)을 사용하여 자궁 경부 염증에 대한 검사 및 dacron swab을 이용하여 phIGFBP-1의 정성 및 정량 분석을 하였다. 이때 nitrazine test를 사용하여 조기 양막 파수가 있는 경우를 제외하였다. phIGFBP-1의 정성 분석은 Medix Biochemica사의 rapid strip test를 사용하였다. 결과 : 조산은 환자군에서 7예가 있었으며, 대조군에서는 한예도 발생하지 않았다 (p=0.037) (Table 2). phIGFBP-1의 정성 분석상 양성이 30예, 음성이 39예이었다. 평균 phIGFBP-1 농도가 양성인 군이 63.5±81.2 g/L, 음성인 군이 1.2±3.7 g/L로 양성인 군이 통계적으로 의미 있게 높았다 (P<0.0001) (Fig. 1). 그러나 이 두 군간의 조산의 발생 빈도는 통계적인 차이를 보이지 않았다 (Table 3). phIGFBP-1의 정량 분석은 양성군에서 83.8±86.5 g/L, 음성군에서 2.1±5.0 g/L로 양성군에서 통계적으로 유의하게 phIGFBP-1의 높은 농도를 보였다 (Table 4). 본 연구 결과에 의하면 phIGFBP-1 검사 kit의 민감도 18.5%, 특이도 59.6%, 위양성률 40.3%, 위음성율 28.6%, 양성예측치 16.7%, 음성예측치 94.9%의 결론을 얻을 수 있었다. 결론 : phIGFBP-1 kit는 검사 결과 산정까지도 단시간에 이루어지며, 검사 방법 및 판독 또한 용이하여 향후 조산 예측의 한 도구로 도움을 줄 수 있으리라 생각된다. 비록 민감도와 특이도가 낮으나 본 연구에서 보인 phIGFBP-1의 높은 음성예측치는 조기 자궁 수축이 있는 산모에 대한 과잉 치료를 예방에 기여할 수 있고, 잠재적인 자궁 수축 억제제의 부작용 또한 낮출 수 있으리라 기대된다. 앞으로 많은 수의 연구가 축적되면 좀 더 낳은 결과가 나오리라 생각된다. Objective : This study is performed to assess whether detection of phosphorylated insulin like growth factor binding protein-1 (phIGFBP-1) in cervical secretions by a bed side test could be used to prediction preterm delivery in patient with regular uterine contraction. Methods : In our prospective study, 42 women between 20.2 and 36.4 weeks gestation with regular, persistent contraction (2-3/min for 30 min). and 27 women between 22.0 and 37.0 weeks of gestation without symptoms of preterm labor were assessed for presence of cervical phIGFBP-1. Dacron swabs were applied to the cervix and assayed in 5min by using immunochromatography, actim partus test (Medix Biochemica). Results : Of the 42 patients with regular uterine contractions, 22 had a positive actim partus test and 20 had a negative test. Among the 22 patients with positive test, 5 delivered preterm and other 17 patients delivered term (<37 weeks). Among the the 20 women with a negative test, 2 delivered preterm (NS). The mean concentration of phIGFBP-1 in 22 positive patients is 83.8±86.5 ㎍/L and that in 20 negative patients is 2.1±5.0 ㎍/L (P<0.0001) (Table 4). Sensitivity, specificity, false positive, false negative, positive predictive, and negative predictive values were 18.5, 59.6, 40.3, 28.6, 16.7, and 94.9%, respectively. Conclusion : The absence of cervical phIGFBP-1 is seems to be a valuable test in excluding preterm delivery in patients who have regular uterine contractions. The high negative predictive value in this study may enable physicians to prevent over treatment of patients with uterine contractions. Therefore, many unwanted side-effects and complications of potentially hazardous tocolytic therapy can be prevented.

고압에 의한 장파열 : 1례 보고 A Case Report

김명찬,백광제,김준식,전영진,한승백 대한외상학회 1998 大韓外傷學會誌 Vol.11 No.1

Rupture of intestine caused by direct pressure from compressed air nozzle is a rare abdominal emergency. Recently, using of compressed gas has been increased and the risk of accident due to improper use of this gas was also increased. We experienced a case of rupture of the colon due to compressed gas delivered through anus as a result of prank. The patient was a 33-year-old man and admitted to the emergency department with severe painful, distended abdomen, and respiratory distress. The patient showed tachypnea, tachycardia, and mild irritability. Supine X-ray film of the abdomen showed marked pneumoperitoneum and elevated diaphragm. After an insertion of the nasogastric tube insertion, decompressive paracentesis to relieve respiratory difficulty was done. After initial management, explo-laparotomy was performed. There was a longitudinal perforation sized by 10cm at antemesenteric border of the sigmoid colon and mucosal and a submucosal tearing of rectal wall sized by 15cm. Primary repair of the rectum and sigmoid colostomy at the site of ruptured portion were performed. The patient was recovered without complication and now waiting for repair of colostomy.

Somatic Embryogenesis and Plant Regeneration from Hypocotyl of Common Buckwheat (Fagopyrum esculentum Moench.)

Myoung-Hae Han,Yoon-Sun Huh,Abu Hena Mostafa Kamal,Sun-Mi Cha,Jung-Sook Bae,Hong-Sig Kim,Sun-Hee Woo 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04

Buckwheat sprout is used as vegetable, and also flour for making noodles, and so on. Currently, information about tissue culture in buckwheat is limited and restricted to micropropagation. We carried out somatic embryogenesis and plant regeneration using hypocotyl segments as explant of the cultivated buckwheat species Fagopyrum esculentum, differs from existing studies in the growth regulator combinations used. Maximum callus regeneration was induced on MS medium containing 2,4-D(2.0 mg/L) and benzylaminopurine BAP (1.0 mg/L) and 3% sucrose. Friable callus was transferred to solidified MS media containing BAP (1.0 mg/L) and at various concentrations for the induction of embryogensis. The optimum concentrations of additives were IAA (2 mg/L), KIN(1.0 mg/L), BAP (1.0 mg/L), and 3% (w/v) sucrose. Only 2,4-D did not show any significant effect on callus induction or embryogenesis. Regeneration of embryonic callus varied from 5 % to 20%. Whole plants were obtained at high frequencies when the embryogenic calluses with somatic embryos and organized shoot primordia were transferred to MS media with 3% sucrose. Regenerated plants after acclimation will transfer to green house. The main objective of this research was to develop a efficient protocol for plant regeneration for common buckwheat, and to apply in future for genetic transformation.

하부급기가 고려된 주방배기후드의 포집효율에 관한 실험적 연구

박명식(Myoung-Sig Park),이대우(Dae-Woo Lee),김정용(Jung-Yong Kim),이성한(Sung-Han Lee) 대한설비공학회 2003 대한설비공학회 학술발표대회논문집 Vol.2003 No.-

자궁경부 생검조직의 병리조직학적 검색

박승창,김달식,임채웅,조남표,강명재,이혜수,최호열,김상호 의과학연구소 1994 全北醫大論文集 Vol.18 No.2

Author evaluated specimens of the uterine cervix submitted in Chonbuk National University Hospital laboratory from Sept. 1983 to Aug. 1986. The specimens were diagnosed hitologically under the light microscope previously. The results were as follows ; 1. The analyzed cervical tissues were 982 cases, e.g. 681 cases by punch biopsy, 166 cases by polypectomy, 32 cases by conization, 51 cases by total hysterectomy, 51 cases by radical hysterectomy, and one cases by endocervical curettage. 2. On the hitological classification of the tissue, chronic cervicities and chronic cervicitis with wquamous metaplasia were 483 cases, cervical polyps 164 cases, dyplasia 123 cases, carcinoma in situ 22 cases, microinvasivee carcinoma 6 cases, invasive squamous cell carcinoma 157 cases, adenocarcinoma 13 cases, condyloma accuninaturn 3 cases and verrucous carcinoma was one case. 3. The peak incidence of cervical lesions occurs 5th decade (365cases), and the average was 44.2 year of age. The youngest and oldest cases were 16 and 79 year of age. 4. The average age of chronic cervicitis was 40.6 years, chronic cervicitis with squamous metaplasia was 40.7 years, cervical polyp was 42.9 years, dysplasia was 42.2 years, carcinoma in sity was 42.8 years, microinvasive carcinoma was 49.8 years, invasive squamous cell carcinoma was 48.4 years, and adenocarcinoma was 50.8 years. 5. Clinical manifestation of the patients with performed biopsy of the uterine cervix were leukorrhea(289 cases), vaginal spotting(279 cases), contact bleeding(229 cases), and irregular vaginal bleeding(221 cases). And other many symptoms were complained. 6. Clinical manifestations of the chronic cervicitis and chronic cervicitis with squamous metaplasia were leukorhea, vaginal spotting and contact bleeding, dysplasia was contact bleeding and leukorrhea, and invasive carcinoma was irregular vaginal bleeding, vaginal spotting, and contact bleeding. 7. The main histological diagnosis of the cervical tissues obtained by punch biopsy(681 cases) were chronic cervicitis and squamous metaplasia(442 cases), invasive carcinoma(128 cases), and dysplasia(85 cases). The tissues by polypectomy(166 cases) were diagnosed with polyp(160 cases) mostly, and conebiopsy tissues(32 cases) were disclosed with dysplasia(16 cases) and chronic cervicitis & squamous metaplasia(10 cases). The specimens by total hysterectomy specimens(51 cases) were confirmed with invasive carcinoma(38 cases) mainly.

Plastic section에서의 효소조직화학적 및 면역조직화학적 방법을 이용한 림프절의 분석

장경현,김달식,임채웅,조남표,강명재,이동근,김상호 의과학연구소 1994 全北醫大論文集 Vol.18 No.2

The present work was designed to evaluate the enzyme histochemical and immunohistochemical characteristics of various cells that constitute lymph node. Lymph nodes were obtained at surgery for the evaluation of lymphadenopathy and were embedded in glycerol methacrylate(plastic) through the complexing tissue processing procedures. Enzyme histochemical examinations('5-nucleotidase, adenosine triphosphatase, acid phosphatase, alkaline phosphatase, nonspecific esterase, and peroxidase) were performed on thin sectioned(2m) tissues. And immunohistochemical stains for IgG and S-100 protein were also performed.

인천항으로 반입되는 휴대농산물에 대한 유해물질 조사

김지형(Ji-Hyeung Kim),조남규(Nam-Gue Cho),이규성(Gue-Sung Lee),주광식(Gwang-Sig Joo),이한정(Han-Jung Lee),황의화(Eui-Hwa Hwang),고종명(Jong-Myoung Go),김용희(Yong-hee Kim) 대한환경위생공학회 2007 대한환경위생공학회지 Vol.22 No.3

본 연구는 인천항으로 유입되는 휴대농산물의 안전성 확보를 위하여 휴대량 초과로 인천본부세관에 유치되는 농산물 등을 검사하였다. 수거 검체는 건고추절편 또는 고춧가루, 곡류, 종실류, 콩류, 견과류, 한약재원료, 참기름 등 총 293건을 대양으로, 품목별로 잔류농약, 곰팡이수, 타르색소, 수단색소 Ⅰ, Ⅱ, Ⅲ, Ⅳ, 아플라톡신, 납, 카드뮴, 수은, 비소, 잔류이산화황 분석을 실시하였다. 1. 건고추절편 또는 고춧가루는 잔류농약동시다성분분석, 곰팡이수, 타르색소, 수단색소 Ⅰ, Ⅱ, Ⅲ, Ⅳ 검사를 실시하였고 이중 곰팡이수와 타르색소에서 부적합이 있었다. 2. 종실류는 타르색소 검사에서 불검출이었고, 콩류와 견과류의 아플라톡신 검사결과도 불검출이었다. 3. 곡류는 납, 카드뮴, 수은의 중금속 검사와 흑미의 경우 타르색소 검사를 추가하였고, 쌀 2건, 찹쌀, 율무, 조에서 각 1건씩 납 기준을 초과하였다. 4. 한약재 원료는 납, 카드뮴, 수은, 비소, 잔류이산화황 검사에서 대추 2건과 건강 3건에서 잔류이산화황이 기준을 초과하였고, 건강 1건에서 카드뮴이 부접합하였다. 5. 추석 전에 대거 유입된 참기름의 규격검사 결과는 모두 적합한 것으로 나타냈다. 6. 총 293건의 휴대농산물 가운데 건고추절편 또는 고춧가루 78건, 곡류 5건, 한약재 5건 등 88건에서 규격기준을 초과하여 30% 부적합율을 나타내었다, 시험항목 중에서는 곰팡이수 부적합 76건, 타르색소 1건, 곰팡이수와 타르색소 동시부적합 1건, 납 5건, 잔류이산화황 4건, 잔류이산화황과 카드뮴 동시부적합 1건으로 나타냈다. 7. 중금속 측정결과는 곡류에서 Pb 0.000~0.740㎎/㎏(0.191±0.209), Cd 0.000~0.108㎎/㎏(0.007±0.020), Hg 0.000~4,201㎎/㎏(0.162±0.319)으로 나타났으며, 한약재원료에서는 Pb 0.000~ 2.480㎎/㎏(1.130±0.730), Cd 0.000~0.761㎎/㎏(0.044±0.122), Hg 0.000~0.157㎎/㎏(0.022±0.033), As 0.000~0.560㎎/㎏(0.040±0.082), 잔류이산화황은 2.0~1459.4ppm(303.1±324.2)으로 나타났다. This study was investigated hazardous substances in agricultural products of baggage by Incheon Port from chinese. We tested 293 kinds of dried red pepper, cereal, sesame, bean, nut, herbal medicine and sesame oil for pesticides residue, mold, tar color, sudan Ⅰ, Ⅱ, Ⅲ, Ⅳ, aflatoxin, Pb, Cd, Hg, As and sulfur dioxide residues. Violated samples over standard limits by Korean Food and Drug Administartion (KFDA) were 76 dried red pepper over the number of mold and 1 dried red pepper detected red 102 tar color and 1 dried red pepper over the number of mold and detected red 102 tar color simultaneously. And 2 rice, 1 sticky rice, 1 adlay and 1 millet were over lead. Violated herbal medicine were 2 Zizyphi Furctus and 2 Zingiberis Rhizoma over sulfur dioxide residue and 1 Zingiberis Rhizoma over sulfur dioxide residue and cadmium simultaneously. The contents of heavy metals in cereal were as follow; Pb 0.000~0.740㎎/㎏(0.19±0.209), Cd 0.000~0.761㎎/㎏(0.044±0.122), Hg 0.000~0.157㎎/㎏(0.022±0.033), As 0.000~0.560㎎/㎏(0.040±0.082). Contents of SO₂ ranged 2.0~1459.4ppm(303.1±324.2).

공동주택에서 발코니 다용도실을 변형한 실외기실의 성능평가

김재수(Jae Soo Kim),박명식(Myoung Sig Park),이건태(Kun Tae Lee),이대우(Dae Woo Lee) 대한설비공학회 2002 대한설비공학회 학술발표대회논문집 Vol.2002 No.-

접종방법에 따른 딸기모무늬병원균의 발병도 검증 및 친환경 방제 효과

김다란(Da-Ran Kim),강근혜(Gun-hye Gang),조현지(Hyun-ji Cho),윤혜숙(Hae-Suk Yoon),명인식(In Sik Myoung),곽연식(Youn-Sig kwak) 한국농약과학회 2016 농약과학회지 Vol.20 No.1