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TC1(C8orf4) is a novel endothelial inflammatory regulator enhancing NF-kappaB activity.
Kim, Jungtae,Kim, Yunlim,Kim, Hyun-Taek,Kim, Dong Wook,Ha, Yunhi,Kim, Jihun,Kim, Cheol-Hee,Lee, Inchul,Song, Kyuyoung Williams Wilkins 2009 JOURNAL OF IMMUNOLOGY Vol.183 No.6
<P>Endothelial inflammation is regulated by a complex molecular mechanism. TC1(C8orf4) is a novel regulator implicated in cancer and inflammation. It is a small protein conserved well among vertebrates. In zebrafish embryos, it is mostly expressed in angio-hematopoietic system and the overexpression induces edema. In human aortic endothelial cells and umbilical vein endothelial cells, TC1 transfection up-regulates key inflammatory cytokines, enzymes, and adhesion proteins including IL-6, IL-1alpha, COX-2, CXCL1, CCL5, CCL2, IL-8, ICAM1, VCAM1, and E-selectin, while TC1 knockdown down-regulates them. TC1 also enhances inflammatory parameters such as monocyte-endothelial adhesion and endothelial monolayer permeability. TC1 is up-regulated by IL-1beta, TNF-alpha, LPS, and phorbol ester, and the up-regulation is inhibited by I-kappaB-kinase inhibitors. TC1, in turn, enhances the nuclear translocation of RelA and the DNA binding activity, suggesting a biological role of amplifying NF-kappaB signaling via a positive feedback. Our findings suggest that TC1 is a novel endothelial inflammatory regulator that might be implicated in inflammatory vascular diseases.</P>
Kim, Jungtae,Kim, Dong Wook,Chang, Wookyoung,Choe, Jongseon,Kim, Jihun,Park, Chan-Sik,Song, Kyuyoung,Lee, Inchul American Association of Immunologists 2012 Journal of Immunology Vol. No.
<P>Follicular dendritic cells (FDCs) protect germinal center (GC) B cells from rapid apoptosis to allow their survival and maturation. In this article, we show that FDCs normally produce and secrete Wnt5a to protect GC B cells. Wnt5a production is upregulated by polyI:C. Purified Wnt5a protects GC B cells from apoptosis in a dose-dependent manner. GC B cells are protected by FDC coculture or conditioned medium, and the protection is inhibited significantly by anti-Wnt5a Ab, suggesting a major role of Wnt5a in the FDC-mediated GC B cell protection. A calcium chelator BAPTA-AM blocks the Wnt5a-mediated GC B cell protection, implying a role of Wnt/Ca(2+) signaling in the GC B cell survival. Wnt5a and calcium ionophore activate NFATc1, NFATc2, NF-κB, and B cell lymphoma 6 (BCL-6) promptly and upregulate CD40 expression in GC B and Ramos cells, whereas p53 and JNK are not upregulated or activated. Cyclosporine A inhibits the Wnt5a and calcium-induced activation of NF-κB and BCL-6 in Ramos cells, supporting a role of β-catenin-independent Wnt/Ca(2+)/NFAT/NF-κB-BCL-6 signaling. Our data support that Wnt5a is a novel survival factor for GC B cells and might be a potential target for the regulation of B cell immunity.</P>
Jungtae Leem,정문주,Sang-hoon Yoon,Hyunho Kim,Hee-Geun Jo,Hyeryun Lee,Jeesu Kim,Hyang Yi Kim,Geun-Woo Kim,Hyung Won Kang 한국한의학연구원 2020 Integrative Medicine Research Vol.9 No.4
Background: Post-traumatic stress disorder (PTSD) has become an important public health problem. However, the conventional therapeutic strategy, including pharmacotherapy and cognitive behavioral therapy, has limitations. Neurofeedback is a technique that utilizes electroencephalography (EEG) signaling to monitor human physiological functions and is widely used to treat patients with PTSD. The purpose of our study is to assess the efficacy and safety level of neurofeedback treatment in patients with PTSD using quantitative EEG. Methods: This is a randomized, waitlist-controlled, assessor-blinded, clinical trial. Forty-six patients with PTSD will be randomly assigned at a 1:1 ratio into two groups. The participants in the treatment group will receive neurofeedback treatment for 50 min, twice a week, for 8 weeks (16 sessions). Quantitative EEG will be utilized to monitor the physiological functions and brain waves of the participants. A four-week follow-up period is planned. The participants in the control group will wait for 12 weeks. The primary outcome is the Korean version of PTSD Checklist-5 (PCL-5-K) score. The PCL-5-K scores on week 8 will be compared between the two groups. Anxiety, depression, insomnia, emotions, EEG, quality-of-life, and safety level will be assessed as secondary outcomes. Discussion: This trial will describe a clinical research methodology for neurofeedback in patients with PTSD. The numerous subjective and objective secondary outcomes add to the value of this trial’s results. It will also suggest a therapeutic strategy for utilizing quantitative EEG in patients with PTSD. Our trial will provide basic evidence for the management of PTSD via an integrative treatment. Trial registration: Clinical Research Information Service (CRIS): KCT0003271.
Enhancement of chitinolytic activity of by co-expression of endochitinase and chitobiosidase genes
Kim, Jungtae,Choi, Shin-Geon 江原大學校 産業技術硏究所 2010 産業技術硏究 Vol.30 No.B
Chitinolytic activity was enhanced by coexpression of endo-chitinase gene (chiA) and chitobiosidase gene (chiB) from Serratia marcescens KFRI314 using constitutive expression vector, pHCEIA, in E. coli. Coexpression vector was constructed by inserting ribosome binding site (RBS) into junction between two chitinase genes. SDS-PAGE analyses showed that two chitinase were constitutively expressed while E. coli clones expressing two chitinases simultaneously increased halo size on colloidal chitin plate. Furthermore, the chitinolytic activities were much enhanced in coexpressed clones when degradation patterns of substrate analogues such as 4-MU-(NAG), 4-MU-(NAG)₂,4-MU-(NAG)₃ were used. Consequently, the combined use of endochitinase and chitobiosidase greatly increased overall chitinolytic activities on recombinant E. coli clones
Kim, Jungtae(김정태) 언어과학회 2015 언어과학연구 Vol.0 No.75
In a Korean context, Self-Directed Learning(SDL) in English language learning is essential, but there is little literature regarding the relationship between SDL and English speaking ability. This study investigated the relationship between Korean college learners` SDL capability and their English speaking proficiency, in particular, the mean difference among the learners` English speaking test scores according to their SDL ability, the effects of gender and study abroad experience on the speaking scores across the SDL levels, and the characteristics of the relationship between the two factors. 90 Korean English language learners 9(ELLs) participated in the study by taking a computerized English speaking test and responding to an SDL survey followed by focal group interviews. The research findings showed that the learners` SDL ability might not be a powerful indicator of their English speaking proficiency; the mean difference of the speaking scores across the SDL groups was significantly different; the effect of study abroad experience was significant across the SDL groups while the gender effect was not. In addition, there was the ELLs` conceptual misunderstanding of the SDL and speaking ability, and their lack of speaking practices. Some suggestions are discussed in terms of improving the ELLs` SDL and English speaking ability.
Fast Capturing on Micromagnetic Cell Sorter
Jungtae Kim,Hyeck-Hee Lee,Steinfeld, U.,Seidel, H. IEEE 2009 IEEE SENSORS JOURNAL Vol.9 No.8
<P>A new micro immune-magnetophoretic cell sorter (micro IMP cell sorter) has been developed to isolate T lymphocytes from biological suspensions such as whole blood. By using two permanent magnets, the developed micro IMP cell sorter is designed to isolate target cells continuously and automatically without a preliminary labeling process which is often acting as a bottleneck in automated microcell sorters. By using the capture probability analysis and the binding kinetic analysis, fast capturing of the target cells was achieved in the straight microfluidic channel between the magnets within 20 s of flow time. Multiphysical simulations including fluidic and electromagnetic models were carried out describing the trajectory of the magnetic particle, also. The fabricated microcell sorter was tested for sorting of CD3+ T lymphocytes with Dynabead CD3 from a mixture of T lymphocytes and erythrocytes.</P>
Kim, Youngmi,Kim, Jungtae,Park, Juhee,Bang, Seunghyun,Jung, Yusun,Choe, Jongseon,Song, Kyuyoung,Lee, Inchul Elsevier 2006 FEBS letters Vol.580 No.14
<P><B>Abstract</B></P><P>Follicular dendritic cells (FDC) play crucial roles in immune regulation. TNF-α has been shown to be essential to the FDC network. However, the molecular regulation of FDC proliferation has not been characterized. Here, we show that TC1(C8orf4), a novel positive regulator of the Wnt/β-catenin pathway in vertebrates, is upregulated by IL-1β and TNF-α in the human FDC-like line HK. TC1 enhances HK cell proliferation, while TC1-knockdown inhibits the proliferation induced by IL-1β, suggesting a role of TC1 as a regulator of FDC proliferation. The regulation by pro-inflammatory cytokines suggests that TC1 might be implicated in linking local inflammation to immune response by stimulating FDC.</P>