백서 척수후근절로부터 슈반세포의 효과적인 체외 배양법

김성민,이종호,김남열,안강민,최원재,최시호,차미주,이주영,황순정,장정원,명훈,최진영,서병무,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

Schwann cells(SCs), an important component of the peripheral nervous system, intract with nerous to mutually support growth and replication for the peripheral nerve regentation. Recently, ading SCs to the lumen of guidance channel is widely tried to improve regeneration or to make regeneration possible over otherwise irreparable gaps. however, it is not easy to isolate and multiplicate SCs as much as enough to help the axonal regeneration. For the allogeneic SCs source for tubular nerve guidance, we developed a little bit improved technique of harvesting and multiplicating SCs. by culturing dispersed dorsal root ganglia in specially designed medium with growth factors and serial processing, we repeatedlly generate relatively homogenous SC cultures. Our technique was compared with other methods of literature using immunostaining methods such as GFAP, S100, BDNF and the total SC count assessment at different time interval after primary culture.

Smart Cell Array Chip Fabrication using Functional Microbeads containing Optical Codes for Self‐identification

Joo?Myung Ahn,Joong Hyun Kim,Ji Hoon Kim,Man Bock Gu 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

Apoptotic Effect of Co-treatment with Chios Gum Mastic and HS-1200 on G361 Human Melanoma Cell Line

Young-Joo Hur,Young-Ki Kim,Hyun-Ho Kwak,Gyoo-Cheon Kim,Seung-Eun Lee,In-Ryoung Kim,Chul-Hoon Kim1,Bong-Soo Park 대한해부학회 2009 Anatomy & Cell Biology Vol.42 No.2

Chios gum mastic (CGM) is a resinous exudate obtained from the stem and the main leaves of Pistacia lenticulus tree native to Mediterranean areas. Recently it reported that CGM induce apoptosis in a few cancer cells in vitro. Bile acids and their synthetic derivatives induced apoptosis in various kinds of cancer cells and anticancer effects. It has been reported that the synthetic chenodeoxycholic acid (CDCA) derivatives showed apoptosis-inducing activity on various cancer cells in vitro. This study was undertaken to investigate the synergistic apoptotic effect of cotreatment with a natural product, CGM and a CDCA derivative, HS-1200 on G361 human melanoma cells. To investigate whether the co-treatment of CGM and HS-1200 compared with each single treatment efficiently reduced the viability of G361 cells, MTT assay was conducted. To investigate augmentation of apoptosis in G631 cells co-treated with CGM and HS-1200, DNA electrophoresis, Hoechst staining, proteasome activity assay, flow cytometry, Westen blot analyses, immunofluorescent staining and confocal microscopy were performed. In this study, G361 cells co-treated with CGM and HS-1200 showed several lines of apoptotic manifestation such as nuclear condensations, DNA fragmentation, the reduction of MMP and proteasome activity, the decrease of DNA content, the release of cytochrome c into cytosol, the translocation of AIF and DFF40 (CAD) onto nuclei, activation of caspase-9, caspase-3, PARP and DFF45 (ICAD), and up-regulation of Bax whereas each single treated G361 cells did not. Although the single treatment of 40 μg/mL CGM or 25 μM HS-1200 for 24 hrs did not induce apoptosis, the co-treatment of them induced prominently apoptosis. Therefore, combination therapy of CGM and HS-1200 could be considered, in the future, as an alternative therapeutic strategy for human melanoma.

Chlorpromazine이 백서 해마에서 전기경련 충격에 의한 MAPK 타이로신 인산화에 미치는 영향

주연호,정성훈,전송희,강웅구,김용식,김상욱,박주배 大韓神經精神醫學會 1997 신경정신의학 Vol.36 No.2

Object : In order to examine the interaction mechanisms of electroconvulsive shock(ECS) and antipsychotic drug at the level of molecular biology, we observed the effect of chlorpromazine pre-treatment on the activation of mitogen activated protein kinase(MAPK) induced by electroconvulsive shock(ECS) in rat hippocampus Method : Male Sprague-Dawley rats were divided into 2 groups. To the experimental group chlorpromazine(20㎎/㎏) was given intraperitoneally, and to the control distilled water was given instead. Thirty minutes later, ECS was given and the hippocampus was dissected out 2 minutes thereafter. Immunoblotting with antiphosphotyrosine antibody was carried out, and the signal intensity at 42kDa band was quantitized using densitometer. The obtained result was compared by student t-test between the experimental and the control group. The absolute amount of MAPK was measured by immunoblotting with anti-MAPK antibody. Result : The tyrosine phosphorylation of MAPK reached peak at 2 minutes after ECS. However, in the chlorpromazine pre-treated group, the peak level of MAPK tyrosine phosphorylation was significantly attenuated(t=-3.12, df=14, p=0.008) compared to the control. In contrast to this, the absolute amount of MAPK did not differ between the pretreated and the control group. Conclusion : Chlorpromazine attenuated the tyrosine phosphorylation of MAPK by ECS. This finding seems to be related to the fact that chlorpromazine pre-treatment changed the cfos expression by ECS in rat brain. Antipsychotic drug and ECS might interact at the level of MAPK signal transduction system, and this might explained the observed synergistic effect of two treatment modality.

상아질 접착제의 전단결합강도와 미세누출에 관한 연구

김정호,조영곤,문주훈 大韓齒科保存學會 1997 Restorative Dentistry & Endodontics Vol.22 No.2

New bonding agent systems have been supplied which operators can simply apply to conditioned tooth surfaces. The purpose of this study was to evaluate the shear bond strengths and the microleakages of three bonding agents and composite resins to dentin. Seventy-five extracted human maxillary and mandibular molar teeth were used in this study. For the shear bond strength test, the entire occlusal dentin surfaces of thirty teeth were exposed with Diamond Wheel Saw and smoothed with Lapping and Polishing Machine (South Bay Technology Co., U.S.A.). For the microleakage test, Class V cavities were prepared in the buccal surfaces of fourty-five teeth. They were randomly assigned into 3 groups according to dentin bonding agents (Scotchbond^TM Multi-Purpose plus, ONE-STEP^TM and Prime & Bond^TM) and composite resins (Z-100, Aelitefil^TM and TPH Spectrum^TM) to be used. Bonding agents and composite resins were bonded to exposed dentin surfaces of the tooth crown and to Class V cavities on the buccal surfaces respectively according o manufacturer's directions. The shear bond strengths were measured by universal testing machine(U^TM AGS-100, Japan). In addition, the degree of microleakage at the occlusal and gingival margin was examined by 2% methylene blue and stereomicroscope(Olymous SZH 10, Japan). The results were as follows: 1. The shear bond strength to dentin was the highest value in SBMP-Plus group(16.68±7.38MPa) and the lowest value in Prime & Bond group(11.61±5.82MPa), but there was no significant difference of shear bond strength among three groups. 2. The degree of microleakage at both occlusal and gingival margin was showed the lowest in SBMP-Plus group and the highest in ONE-STEP group. 3. At both occlusal and gingival margin, there was significant difference of microleakage between SBMP-Plus and ONE-STEP/Prime & Bond groups(p<0.05), but no significant difference of microleakage between ONE-STEP and Prim & Bond group(p>0.05).

특수학교의 재구조화 방안

김주영,김병하,이미선,이유훈 국립특수교육원 2000 연구보고서 Vol.- No.2

통합교육은 특수학교 중심 의 전통적 인 분리교육에 대응한 개념으로 이해 할 수 있다. 1994년 특수교육진흥법이 전면 개정되면서 우리나자는 장애학생 교육의 전달체계를 통합교육을 촉진하는방향으로 개 혁해야 한다는 수장이 대두되 기 시작하였다. 특수교육 전달체 계 의 개 혁은 곧 분리교육의 해체를 뜻하는 것이라고 여겼기 때문이다. 이와 같은 관점에서 현재 특수교육 전달체계 가운데가장 큰 개혁의 초점은 바로 특수학교가 될 것이다. 왜냐하면, 그동안 특수학교는 분리교육의 중심으로 여겨져 왔을 뿐만 아니라, 가정이나 병원, 시설에서 이루어지는 장애학생의 교육도 결국은그 거점이 특수학교가 될 가늘성이 높기 때문이다. 그러므로 특수교육이 기존의 분리교육 체제를해체하고 통합교육의 체제로 옮아가기 위해서는 필연적으로 특수학교의 역할과 기능을 제고함으로써 통합교육 체제에 순응하고 적극 기 여 할 수 있는 전달체 계로서 의 새로운 특수학교 구조를 제시할 필요가 있는 것이다. 그것은 바로 바로 특수학교의 재구조화를 의미하는 것이다. 이에 따라,본고에서 제시하고 있는 우리나라 특수학교의 재구조화 방향과 그에 따른 방안은 다음파 같다.특수학교 재구조화 방향한국적인 상황에서 급진적 통합교육의 추진은 자칫 장애학생의 행복을 저해하고 장애의 특성과정도에 따른 다양한 교육서비스의 선택 폭을 줄일 뿐만 아니라, 기존의 엄청난 특수교육 자원을낭비할 수도 있다는 것이 현재 특수교육계의 지배적인 생각이다. 따라서 특수학교는 앞으로 중증 · 중복장애 학생의 교육 기 관화와 지 역사회 중심 의 특수교육 센터화, 특수교육의 특성화와 전문화의 길로 나아가는 것이 바람직할 것으로 기대된다.특수학교 재구조화 방안 I : 특수학교의 운영 체제 전환첫째, 특수학교는 장애의 진단 평가 및 상담과 중중 ·중복장애차생 교육, 재택 순회교육, 일반학교의 특수교육 지원, 지역사회 장애이해 증진 등에 걸쳐 그 학교의 역할을 제고해야 한다둘째,특수학교는 일반학교 내에 특수학교를 두는 형태, 특수학교에 일반학생들을 받아들이는형태, 장애영역을 통합하는 형태, 작은 규모의 형태, 경도장애학생들을 위한 실업 고등학교 등 학교의 형태를 다양화해야 한다. 셋째,특수학교는 교육과정을 생활기능 중심,통합교과 중심의 총체적 교수방법 적용,개별화교육계획(IEP)의 탄력적 적용 등을 제고해야 한다.넷째, 특수학교는 교원의 특수교육-일반교육 근무체제를 활성화하고, 특수교육 담당 교사에 대해 우대하며, 우수인력의 고른 배치와 현직교원의 교차 연수기회를 부여함으로써 교원의 자질과전문성을 신장해 야 한다.다섯째, 특수학교는 지역내 순회교육 대상 장애학생들의 교육을 중점적으로 담당해야 한다.석섯째,특수학교는 학생 중심,지역사회 적응 중심의 전환교육을 바탕으로 한 직업교육을 체계적으로 실시해야 한다.일곱째,특수학교는 부모의 의견을 수렴하여 학교 교육과정 운영에 적절히 반영하는 한편,자녀의 발달단계에 따른 발달과업을 중심으로 부모교육을 실시해야 한다.특수학교 재구조화 방안 ll : 국가 및 지방자치단체의 변화국가 및 지방자치단체는 새로운 조직이나 사업 개발에 착수하기보다는 기존의 조직과 사업을활용하면서 교원 각자의 참여를 유도할 수 있는 통합교육 정책 개발과 투자에 힘을 쏟아야 하며,지방 특수교육의 안정적 발전을 위해 지속적으로 예산 지원을 보장해야 한다. 또한 예산의 안정적지원과 함께 국가는 지방특수교육에 대한 평가를 정례화 하여 그 성과에 따라 특별교부금을 지급하거나 적정 투자권고 등 행정지도를 강화해야 한다.특수학교 재구조화 방안 In : 지역사회 중심의 특수학교 체제첫째, 특수학교는 학교간 교류협력의 활성화와 지역사회 자원을 활용한 경험 중심의 교윽과정의 실천,지역사회 자원을 활용한 직업교육의 실시,자원봉사 인력의 활용 등을 통한 지역사회 자원을 활용한다.둘째, 특수학교는 학교 시설의 제공, 각종 문화행사, 장애인 및 그 가족을 지원함으로써 지역사회 센터로서 기여해야 한다.지금까지 제시한 특수학교 재구조화의 방향과그에 따른 방안들은 우리나라의 전통적 특수교육상황을 적극적으로 개선하기 위해 반영한 제 안들이 다. 그러나 이 러 한 방안을 제시하는 것만으로특수학교의 재구조화가 실현되는 것은 아니다. 국가는 국가수준에서, 학교는 학교수준에서 이미제시된 방향을 향해 구체적인 실천과제를 정하여 하나 하나 구체적으로 실천해 갈 때 비로소 가능해 지는 것임을 잊어서는 안될 것이다.

상부소화관출혈에 있어서 내시경적 분말약제산포의 효과

김상균,조주영,송상훈,김진오,이준성,이문성,심찬섭 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Background : Bleeding after endoscopic procedure of the stomach carries an increased risk of morbidity and mortality. In case of oozing hemorrhage, not in active hemorrhage, powdered sodium alginate may play a role in control of bleeding with convenience. Therefore, we evaluated the clinical effect and efficacy of sodium alginate powder in upper G-Ⅰ bleeding. Methods : Forty-five patients who had upper G-Ⅰ bleeding after endoscopic procedure were enrolled in this study. Twenty-five patients of them were managed by the application of powder preparation of Sodium alginate(ALTO). The others were observed without treatment. The next day, we performed endoscopy in all patients to ascertain the hemostasis. Results : Of 25 patients managed with Sodium alginate, only two patients(8%) had bleeding (hemostatic rate : 92%), On the other hand, of 20 patients who did not receive any treatment, 3 patient(15%) had bleeding (hemostatic rate : 85%). There was no statistical difference between the two groups(p>0.05). Using other endoscopic procedure, We successfully treated the patient who continued to bleed. Conclusion : We evaluated the effect of the spary of sodium alginate on oozing hemorrhage. Although there was no statistical significance in our study, it seems that the spray of sodium alginate may be a useful method for the prevention of bleeding after endoscopic procedure.

성장기 아동에서의 기능성 전치부 개교의 치료증례

김종철,장기택,손동수,김주훈 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.3

Anterior openbite is defined as the lack of contacts between the functional occluding teeth on vertical line at centric occlusion and classified into functional and skeletal anterior openbite based on its causes and characteristics. Anterior openbite causes masticatory, speech, and esthetic problems in the growing children and difficulties in diagnosis, treatment, and the prediction of its prognosis. We are reporting on the treatment of anterior openbite in the growing children and the results follow as : 1. In the growing children with anterior openbite, the overbite could be increased by the treatment according to its causes and characteristics. 2. The prognosis is not determined by the presence or severity of oral habit but the skeletal tendency of the patient.

An analysis on supporting programs of procuring goods for export in Korea

Kim,Jae Seung,Park,Se Hoon,Choi,Young Joo 한국무역상무학회 2010 貿易商務硏究 Vol.48 No.-

To promote the nation's exports, in Korea several government led programs are alive for procuring and manufacturing goods for export. The trade supporting programs are consisted of Trade Financing Program, Trade Tax Program, and Trade Insurance. The programs are summarized ; First, Trade Financing Program is available to exporters of procuring raw materials and finished goods for export. It is extended with exceptionally low interests from the Korean commercial banks. Second, Trade Tax Program provides whole or part of customs drawback to exporters. customs drawback is` made when exporters meet requirements of Customs for export. Third, Trade Insurance Program cover risks of uncertainty which is beyond the exporters' capacity at low insurance premium It is suggested that the exporters have deep knowledge of these three programs to their ends. Especially Trade Financing Program and Trade Insurance Program can be a combined work for the financially weak SMEs. Further, there are rooms for these programs to develop to enhance the competitiveness of SME exporters.

Generalized variational inequalities on H-spaces

김훈주 대불대학교 1995 論文集 Vol.1 No.2

위상선형공간의 볼록공간집합의 개념을 일반화하는 연구가 KKM 이론, 부동점이론, 일치점이론등에서 매우 활발하였다. 그 중 Horvath에 의해 도입된 H-공간상에서 여러가지 변분부등식을 얻었다. 이 변분부등식은 Ding 과 Tan, Kim 과 Tan, Park Browder 등의 연구를 일반화한 것이다. In this note, we prove some variational inequalities which generalize Ding and Tan [DT], Kim and Tan [KT], Park [P2] and Browder [Br].