Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Curcumin protects retinal pigment epithelial cells against oxidative stress via induction of heme oxygenase-1 expression and reduction of reactive oxygen

Woo, Je Moon,Shin, Da-Yong,Lee, Sung Ju,Joe, Yeonsoo,Zheng, Min,Yim, Jin Ho,Callaway, Zak,Chung, Hun Taeg Molecular Vision 2012 Molecular vision Vol.18 No.-

<P><B>Purpose</B></P><P>To determine whether curcumin induces expression of the defensive enzyme heme oxygenase-1 (HO-1) and protects cells against oxidative stress in cultured human retinal pigment epithelial cells.</P><P><B>Methods</B></P><P>Effective concentrations and toxicities of curcumin were determined after 3 h of curcumin treatment with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Confluent human retinal pigment epithelium cell lines (ARPE-19) were preincubated with curcumin and oxidatively challenged with H<SUB>2</SUB>O<SUB>2</SUB>. HO-1 expression was determined with western blot analysis. To confirm the protective role of HO-1 in oxidative stress, small interfering RNA (siRNA) against HO-1 or inhibitor of HO-1 was treated with curcumin in retinal pigment epithelium cells. Intracellular levels of reactive oxygen species (ROS) were measured with flow cytometry and confocal microscopy. Apoptosis was evaluated with Annexin V-fluoroscein isothiocyanate staining.</P><P><B>Results</B></P><P>Curcumin had little cytotoxicity at concentrations less than 30 μM, and HO-1 expression was the highest at the 15 μM concentration. At this concentration, curcumin also increased the cytoprotective effect against the oxidative stress of H<SUB>2</SUB>O<SUB>2</SUB> through the reduction of ROS levels in human retinal pigment epithelial cells. Curcumin’s effect on the reduction of ROS was mediated by the increase in HO-1 expression.</P><P><B>Conclusions</B></P><P>Curcumin upregulated the oxidative stress defense enzyme HO-1 and may protect human retinal pigment epithelial cells against oxidative stress by reducing ROS levels.</P>

아산시 일대 수계의 수환경 요인 변화

신현철,조상호,이충근,현진오 순천향대학교 기초과학연구소 1997 순천향자연과학연구 논문집 Vol.3 No.2

To understand the changes of water quality according to watershed, the four physiochemical characters of water quality, including water temperature, pH, electric conductivity and dissolved oxygen, were investigated from 3 July 1997 to 22 August 1997 at 122 sites in Asan city, Chungnam, Korea. Water temperature ranged from 16.8℃ to 35℃, pH from 6.4 to 10.6, dissolved oxygen from 3.5 mg/l to 19.4 mg/l, and electric conductivity from 6.2 mS/m to 66.3 mS/m. Among 4 characters, the electric conductivity did not show the significant differences between running water and still waters, like reservoir or lakes. only. These results suggest that the water quality around Asan city were worsened by the regional output of pollutant.

18S-srRNA 분석에 의한 Acantbamoeba 한국 분리주들의 분류 검토

신호준,지영진,조명수,김형일,박연희,임경일 아주대학교 의과학연구소 1998 아주의학 Vol.3 No.2

Acanthamoeba spp. spread in soil, ponds, air and swimming pool, cause a granulomatous amoebic meningitis and amoebic keratitis in human and experimental animals. Their classification had depended upon morphological characteristics and physiological conditions such as thermophilic status and generation times. However, because amoeba species showed the morphological and physiological diversity in isolates, many attempts have been made on identification of Acanthamoeba spp. Restriction fragments length polymorphism (RFLP) analysis of IBS-small subunit ribosomal RNA (srRNA) was an useful tool for classification among morphologically and genetically closely-related species. In this study, 18S-srDNAs of amoebae were amplified by PCR with primer encoding 18S-srRNA gene and digested with restriction endonudeases, and RFLP analysis was applied on classification of Acanthamoeba Korean isolates (YM-4, YM-5 and YM-7), comparing with reference amoebae, A. culbertsoni, A. polyphaga and A. royreba. Trophozoites of six Acanthamoeba spp. had typical acanthopoda, but did not show any morphological differences. Cyst of Acanthamoeba sp. YM-7 was similar to that of A. polyphaga which was designated as group Ⅱ Acanthamoeba. Morphologically other amoebae belonged to group Ⅲ Acanthamoeba. Digestion of 18S-srDNA with six enzymes, Dde Ⅰ, Hae Ⅲ, Hind Ⅲ, EcoR Ⅰ, Rsa Ⅰ and Sph Ⅰ, resulted in various DNA fragments. Using the method of Nei and U (1979) for RFLP analysis, genetic divergence was not observed between A culbertsoni and Acanthamoeba sp. YM-4. Between A. culbertsoni and Acanthamoeba sp. YM-5, genetic distance was 0.070, 0.364 between Acanthamoeba sp. YM-4 and A. polyphaga, and 0.277 between Acanthamoeba sp. YM-7 and A. polyphaga. In comparison with A. culbertsoni and A. polyphaga, Acanthamoeba sp. YM-7 showed genetic distance of 0.330 and 0.154, respectively. Thus, Acanthamoeba sp. YM-7 is a similar species to A. polyphaga. Acanthamoeba sp. YM-5 is a different species from A. polyphaga, but closely-related to A. culbertsoni. Acanthamoeba sp. YM-4 can be classified as a subspecies or a strain of A. culbertsoni.

고성능 표면탄성파 필터의 최적 설계에 관한 연구

신양호,김귀현,이진복,엄현석,이중근,박진석 漢陽大學校 工學技術硏究所 1999 工學技術論文集 Vol.8 No.1

본 논문에서는 고성능 표면탄성파 필터의 최적 설계를 위하여 다양한 IDT(interdigital transducer) 구조의 필터를 제작하고 각각의 주파수 응답특성을 비교 분석하였다. 기본 구조외에 MSC(micro strip-coupler), double split, reflector, ground line 등의 구조를 일반적으로 널리 사용되고 있는 Y-Z cut l-N(LiNbO3) 기판 위에 형성시켰다. 제작된 소자의 중심주파수, 통과 대역, 삽입손실등을 측정하였으며, 주파수 응답에 기초한 기존 모델의 효용성을 논의하였다. Effects of IDT structures on frequency responses of SAW filters are investigated to achieve a high performance SAW filter, Various types of IDTS, such as MSC(multistrip-coupler), double split, reflector, and ground line, are designed and patterned on a widely used Y-Z cut LN(LiNbO3) substrate. Center frequencies, band widths, and insertion losses are measured and compared. Invalidity of the conventional model based on the impulse response model is also discussed.

LCA 기법을 적용할 제품의 우선순위 선정 방법론

신동희,권은선,문승식,김만영,정종연,허진호 한국전과정평가학회 2004 한국전과정평가학회지 Vol.5 No.1

본 연구의 목적은 일정한 논리적 절차를 거쳐서 객관적인 전과정평가의 우선순위를 선정하기 위한 일반적인 방법론의 개발이다. 우선 전과정평가 대상이 되는 제품(산업군)의 우선순위를 평가하기 위해서 제품의 환경성과 관련된 환경규제, 국내외 전과정평가 수행사례, 유해물질 등을 평가항목으로 설정하였다. 설정된 평가항목들에 대한 자료조사 및 분석을 통하여 세분화한 데이터베이스를 생성하였으며, 또한 학계 및 산업계의 의견을 반영하기 위해 전과정평가 전문가 및 산업계 관계자들을 대상으로 설문조사를 실시하였다. 설문조사결과를 바탕으로 각각의 평가항목 및 세부평가항목의 가중치를 부여하였고, 각 평가항목별로 점수를 설정하였다. 본 연구에서 개발한 방법론은 우선순위를 비교해야하는 제품(산업군)들이 각각의 평가항목에서 설정해놓은 해당 점수를 부여받게 된다. 부여받은 각 평가항목의 점수들을 모두 합산하면 평가대상이 되는 제품(산업군)에 대해 하나의 우선순위 평가 점수를 갖게 된다. 이와 같은 과정으로 방법론이 구축되었으며, 사례연구를 통하여 방법론의 타당성을 검증하였다. 이렇게 산출된 우선순위 평가점수를 평가하고자하는 제품(산업군)들과 비교하여 높은 점수를 갖는 제품(산업군)이 전과정평가를 먼저 실시하는 것이 바람직하다는 결론을 얻을 수 있다. The purpose of this study is to develop a general method to decide the priority of LCA application through a logical procedure. First of all, evaluation categories were developed to decide priority of subject products(industrial groups). The evaluation categories consist of environment regulations related to subject product, LCA case studies, hazardous substances, etc. The subdivided database was constructed through researching and analyzing information about the selected evaluation categories. In addition, a questionnaire was conducted to reflect the opinions from academic and industrial circles. Based on the result of the questionnaire, each evaluation category and sub-evaluation category were weighed differently, and certain points were assigned to them. According to the method developed in this study, comparing industrial groups or products for prioriting will have its total score that is a sum of points assigned to each evaluation category. This results that an industrial group or product having higher score than the others becomes the subject to apply LCA.

고주파 가열에 따른 반응고 알루미늄합금의 미세조직 특성

신평우,이정환,이상용,노진호,이영선 國立 昌原大學校 産業技術硏究所 1997 産技硏論文集 Vol.11 No.-

Semi-solid forming is relatively new process for forming alloys in the semi-solid condition into near net produce. It relies on the behaviour of semi-solid slurrys in which the solid exists in the form of non-dendritic, spheroidal particles in a liquid matrix. Powder of the Al-20Si-5Fe-2Ni alloy was manufactured by spray forming and powder metallurgy, one of the rapid cooling method, and its microstructure became fine with several ㎛ size through the extrusion process. Spray forming and powder metallurgy are a rapid solidification process in which capable of producing materials consisted of very fine, uniform particles. Spray forming can be described as incorperating the advantages of powder metallurgy without disadvantages of degassing and consolidation. In this paper, the microstructural evolution of the P/M, S/F Al-20Si-5Fe-2Ni alloys and wrought Al 2024 alloys were investigated various heating conditions by high frequency induction heating system.

VHDL을 이용한 실시간 JPEG 인코더 설계

신인철,어진우,강호준 단국대학교 1997 論文集 Vol.31 No.-

Image compression techniques have been necessary to transfer and save a great amount of image data in an efficient way. Because the compression and decompression of image signal require the real time processing, various hardware implementation methods have been studied. In this paper we propose the algorithm which is parallel form of processing 2 dimensional 8*8 blocks simultaneously, so increase speeds. It outputs compressed bit sequences 8 clocks later for the input data synchronized to clocks. Proposed algorithm is designed using VHDL. Simulation and circuit synthesis has been performed by the Powerview CAD Tool on the SUN Sparc-10 workstation. Results shows that maximum operating frequency is up to 15㎒. The final schematic circuit and comparison of processing speed are shown.

요양소에 수용중인 정신장애자의 입소전 치료방법에 관한 연구

신병호,윤진상,이충경,이형영 大韓神經精神醫學會 1988 신경정신의학 Vol.27 No.5

In Korea, a large number of the mentally ill are still institutionalized in asylums where they can not receive modern psychiatric treatments. This survey was designed to investigate previous treatment methods of these psychiatric patients and to offer basic data in order to develop modern psychiatric delivery system for patients institutionalized in asylums. The 694 subjects who could give cooperative informations wee selrected form 6 asylums which are located in the Kwang-ju and Chonnam area. The data on this survey was collected by investigator's interview from Mar. 30, 1987 to Apr. 10, 1987. The previous treatment methods were divided into 8 groups(modern psychiatric treatment, asylym, Korean shamanic rite, religious healing, herb medicine, drug store, others and no treatment) and multiple responses were allowed. The methods were studied relating to the following psychiatric and sociodemographic factors : psychiatric diagnosis, age at the time of institutionalization, morbid duration before institutionalization, sex, occupation, residence, religion, economic status, marital status, educational level, and guardian. 49% of the patients had no experienced any previous treatment for their mental illness. 35% of the patients had been given modern psychiatric treatment and 23% had been previously institutionalized in asylums. The other treatment methods were reported in less than 10% of the patients. There were significant relationships between the choice of treatment methods and the psychiatric and sociodemographic factors.