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A synthetic study and characterization of the Pt(II) complexes with bipyridines back-born system.
Jo, Woongkyu,Son, Seokhwan,Jo, Hyeongjun,Kim, Byeongcheol,Kwak, Cheehun,Jung, Sangchul,Lee, Jihoon,Ahn, Hogeun,Chung, Minchul American Scientific Publishers 2014 Journal of Nanoscience and Nanotechnology Vol.14 No.8
<P>The reaction of platinum [Pt(5,5-dmbpy)]Cl2 (5,5-dmbpy = 5,5'-dimethyl-2,2'-bipyridine) with 4,4'-dimethyl-2,2'-bipyridine (4,4-dmbpy), [Pt(dbbpy)]Cl2 (dbbpy = 4,4'-dibutyl-2,2'-bipyridine), [Pt(dpbpy)]Cl2 (dpbpy = 4,4'-dipentyl-2,2'-bipyridine) with 5,5'-dimethyl-2,2'-bipyridine (5,5-dmbpy) affords the following complexes: [(4,4-dmbpy)Pt(5,5-dmbpy)][PF6]2 (1) and [(dbbpy)Pt(5,5-dmbpy)][PF6]2 (2), [(dpbpy)Pt(5,5-dmbpy)][PF6]2 (3), [(5,5-dmbpy)Pt(5,5-dmbpy)][PF6]2 (4). This study was synthesized new platinum complex compounds utilizing ligand of 5,5'-Dimethyl-2,2'-dipyridyl System. To study the chemical composition was used 1H(13C)-NMR, UV-vis, Spectro photometer and Measurements about optical physics and chemical properties were measured to use spectrofluorometer. UV-vis absorption area was measured 310 nm to 383 nm and luminous wavelength was measured 390 nm to 419 nm.</P>
Jihoon Jo,Hyun-Gwan Lee,Kwang Young Kim,Chungoo Park 한국조류학회I 2019 ALGAE Vol.34 No.1
DNA metabarcoding is currently used for large-scale taxonomic identification to understand the community compositionin various marine ecosystems. However, before being widely used in this emerging field, this experimental andanalytic approach still has several technical challenges to overcome, such as polymerase chain reaction (PCR) bias, andlack of well-established metabarcoding markers, a task which is difficult but not impossible to achieve. In this study, wepresent an adapted PCR-free small-organelles enriched metagenomics (SoEM) method for marine biodiversity assessment. To avoid PCR bias and random artefacts, we extracted target DNA sequences without PCR amplification from marineenvironmental samples enriched with small organelles including mitochondria and plastids because their genomesequences provide a valuable source of molecular markers for phylogenetic analysis. To experimentally enrich smallorganelles, we performed subcellular fractionation using modified differential centrifugation for marine environmentalDNA samples. To validate our SoEM method, two marine environmental samples from the coastal waters were tested thetaxonomic capturing capacity against that of traditional DNA metabarcoding method. Results showed that, regardless oftaxonomic levels, at least 3-fold greater numbers of taxa were identified in our SoEM method, compared to those identifiedby the conventional multi-locus DNA metabarcoding method. The SoEM method is thus effective and accuratefor identifying taxonomic diversity and presents a useful alternative approach for evaluating biodiversity in the marineenvironment.
Jo, Jihoon,Lee, Hyun-Gwan,Kim, Kwang Young,Park, Chungoo The Korean Society of Phycology 2019 ALGAE Vol.34 No.1
DNA metabarcoding is currently used for large-scale taxonomic identification to understand the community composition in various marine ecosystems. However, before being widely used in this emerging field, this experimental and analytic approach still has several technical challenges to overcome, such as polymerase chain reaction (PCR) bias, and lack of well-established metabarcoding markers, a task which is difficult but not impossible to achieve. In this study, we present an adapted PCR-free small-organelles enriched metagenomics (SoEM) method for marine biodiversity assessment. To avoid PCR bias and random artefacts, we extracted target DNA sequences without PCR amplification from marine environmental samples enriched with small organelles including mitochondria and plastids because their genome sequences provide a valuable source of molecular markers for phylogenetic analysis. To experimentally enrich small organelles, we performed subcellular fractionation using modified differential centrifugation for marine environmental DNA samples. To validate our SoEM method, two marine environmental samples from the coastal waters were tested the taxonomic capturing capacity against that of traditional DNA metabarcoding method. Results showed that, regardless of taxonomic levels, at least 3-fold greater numbers of taxa were identified in our SoEM method, compared to those identified by the conventional multi-locus DNA metabarcoding method. The SoEM method is thus effective and accurate for identifying taxonomic diversity and presents a useful alternative approach for evaluating biodiversity in the marine environment.
Jo, Jihoon,Heon, Seok,Kim, Myung Jong,Son, Gi Hoon,Park, Yunkyung,Henley, Jeremy M.,Weiss, Jamie L.,Sheng, Morgan,Collingridge, Graham L.,Cho, Kwangwook Elsevier 2008 Neuron Vol.60 No.6
<P><B>Summary</B></P><P>There are two major forms of long-term depression (LTD) of synaptic transmission in the central nervous system that require activation of either N-methyl-D-aspartate receptors (NMDARs) or metabotropic glutamate receptors (mGluRs). In synapses in the perirhinal cortex, we have directly compared the Ca<SUP>2+</SUP> signaling mechanisms involved in NMDAR-LTD and mGluR-LTD. While both forms of LTD involve Ca<SUP>2+</SUP> release from intracellular stores, the Ca<SUP>2+</SUP> sensors involved are different; NMDAR-LTD involves calmodulin, while mGluR-LTD involves the neuronal Ca<SUP>2+</SUP> sensor (NCS) protein NCS-1. In addition, there is a specific requirement for IP3 and PKC, as well as protein interacting with C kinase (PICK-1) in mGluR-LTD. NCS-1 binds directly to PICK1 via its BAR domain in a Ca<SUP>2+</SUP>-dependent manner. Furthermore, the NCS-1-PICK1 association is stimulated by activation of mGluRs, but not NMDARs, and introduction of a PICK1 BAR domain fusion protein specifically blocks mGluR-LTD. Thus, NCS-1 plays a distinct role in mGluR-LTD.</P>
Jihoon Jo,오주성,Chungoo Park 한국미생물학회 2020 The journal of microbiology Vol.58 No.3
Microbial communities present in diverse environments from deep seas to human body niches play significant roles in the complex ecosystem and human health. Characterizing their structural and functional diversities is indispensable, and many approaches, such as microscopic observation, DNA fingerprinting, and PCR-based marker gene analysis, have been successfully applied to identify microorganisms. Since the revolutionary improvement of DNA sequencing technologies, direct and high-throughput analysis of genomic DNA from a whole environmental community without prior cultivation has become the mainstream approach, overcoming the constraints of the classical approaches. Here, we first briefly review the history of environmental DNA analysis applications with a focus on profiling the taxonomic composition and functional potentials of microbial communities. To this end, we aim to introduce the shotgun metagenomic sequencing (SMS) approach, which is used for the untargeted (“shotgun”) sequencing of all (“meta”) microbial genomes (“genomic”) present in a sample. SMS data analyses are performed in silico using various software programs; however, in silico analysis is typically regarded as a burden on wet-lab experimental microbiologists. Therefore, in this review, we present microbiologists who are unfamiliar with in silico analyses with a basic and practical SMS data analysis protocol. This protocol covers all the bioinformatics processes of the SMS analysis in terms of data preprocessing, taxonomic profiling, functional annotation, and visualization.
Muscarinic receptors induce LTD of NMDAR EPSCs via a mechanism involving hippocalcin, AP2 and PSD-95
Jo, Jihoon,Son, Gi Hoon,Winters, Bryony L,Kim, Myung Jong,Whitcomb, Daniel J,Dickinson, Bryony A,Lee, Youn-Bok,Futai, Kensuke,Amici, Mascia,Sheng, Morgan,Collingridge, Graham L,Cho, Kwangwook Nature Publishing Group, a division of Macmillan P 2010 NATURE NEUROSCIENCE Vol.13 No.10
Although muscarinic acetylcholine receptors (mAChRs) and NMDA receptors (NMDARs) are important for synaptic plasticity, learning and memory, the manner in which they interact is poorly understood. We found that stimulation of muscarinic receptors, either by an agonist or by the synaptic release of acetylcholine, led to long-term depression (LTD) of NMDAR-mediated synaptic transmission. This form of LTD involved the release of Ca<SUP>2+</SUP> from IP<SUB>3</SUB>-sensitive intracellular stores and was expressed via the internalization of NMDARs. Our results suggest that the molecular mechanism involves a dynamic interaction between the neuronal calcium sensor protein hippocalcin, the clathrin adaptor molecule AP2, the postsynaptic density enriched protein PSD-95 and NMDARs. We propose that hippocalcin binds to the SH3 region of PSD-95 under basal conditions, but it translocates to the plasma membrane on sensing Ca<SUP>2+</SUP>; in doing so, it causes PSD-95 to dissociate from NMDARs, permitting AP2 to bind and initiate their dynamin-dependent endocytosis.
상용 CFD코드를 이용한 가정용 연료전지용 원심펌프 유동해석에 관한 연구
조지훈(Jihoon Jo),신동훈(Donghoon Shin),정태용(Tae Yong Chung) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.11
In this study, compared efficiency of centrifugal pump and internal flow characteristics of centrifugal pump according to rotation speed on same conditions. A numerical study of a centrifugal pump is carried out using CFD commercial code, Fluent 6.3.26. The oultet pressure is fixed by 20[㎪] and was performed to analyze for 3 impeller geometry. The rotation speed of impeller analyzed dividing into four case, 400[rad/s], 500[rad/s], 600[rad/s], 700[rad/s]. If curve of impeller increased, stagnation phenomenon is increased in internal of impeller. Also, appeared reversing flow at internal of impeller. As a result, we are selecting the most efficient rotation speed in each proto type. In this study, the obtained result will be utilizing as important design data.