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Identification and characterization of presence/absence variation in maize genotype Mo17
Lu Jiang,Yuanda Lv,Tan Li,Han Zhao,Tifu Zhang 한국유전학회 2015 Genes & Genomics Vol.37 No.6
Presence/absence variation (PAV), a major class of genome structure variation, is pervasive in the maize genome. PAVs present in the B73 but absent from the Mo17 genome have been reported in previous studies. Here, the next-generation sequencing was used to identify the PAVs present in Mo17 but absent from B73. A total of 119 PAVs were identified, of which 57 were validated by PCR. Using the intermated B73 9 Mo17 segregating population, 57 validated PAVs were mapped into the genetic map. These PAVs were dispersed on the ten chromosomes. Also, several large genetic regions of PAVs were identified. This suggested the possibility that large genetic fragments in Mo17 were absent from the B73 genome. Several PAVs were also found to be related to disease resistance suggesting that presence/absence variations in the genome may play a role in disease resistances in maize. In addition, the majority of the genes within PAV were transcriptionally silenced or expressed at low levels in some tissues, especially for PAVs related to disease resistance. The results of this study not only provided the identities of additional PAVs in maize but also helped understand the functional role of PAVs in this agriculturally important crop.
Current Status of Etiology, Epidemiology, Clinical Manifestations and Imagings for COVID-19
Jiang Meng Di,Zu Zi Yue,Schoepf U. Joseph,Savage Rock H.,Zhang Xiao Lei,Lu Guang Ming,Zhang Long Jiang 대한영상의학회 2020 Korean Journal of Radiology Vol.21 No.10
Coronavirus disease 2019 (COVID-19) is a transmissible respiratory disease that was initially reported in Wuhan, China in December 2019. With the alarming levels of COVID-19 spread worldwide, the World Health Organization characterized COVID-19 as a pandemic. Over the past several months, chest CT has played a vital role in early identification, disease severity assessment, and dynamic disease course monitoring of COVID-19. The published data has enriched our knowledge on the etiology, epidemiology, clinical manifestations, and pathologic findings of COVID-19. Additionally, as the imaging spectrum of the disease continues to be defined, extrapulmonary infections or other complications will require further attention. This review aims to provide an updated framework and essential knowledge with which radiologists can better understand COVID-19.
Jiang Wan-Zhu,Yao Fang-Jie,Lu Li-Xin,Fang Ming,Wang Peng,Zhang You-Min,Meng Jing-Jing,Lu Jia,Ma Xiao-Xu,He Qi,Shao Kai-Sheng 한국미생물학회 2021 The journal of microbiology Vol.59 No.1
Gloeostereum incarnatum is an edible medicinal mushroom widely grown in China. Using the whole genome of G. incarnatum, simple sequence repeat (SSR) markers were developed and synthetic primers were designed to construct its first genetic linkage map. The 1,048.6 cm map is composed of 10 linkage groups and contains 183 SSR markers. In total, 112 genome assembly sequences were anchored, representing 16.43 Mb and covering 46.41% of the genome. Selfing populations were used for quantitative trait loci (QTL) targeting, and the composite interval mapping method was used to co-localize the mycelium growth rate (potato dextrose agar and sawdust), growth period, yield and fruiting body length, and width and thickness. The 14 QTLs of agronomic traits had LOD values of 3.20–6.51 and contribution rates of 2.22– 13.18%. No linkage relationship was found between the mycelium growth rate and the growth period, but a linkage relationship was observed among the length, width and thickness of the fruiting bodies. Using NCBI’s BLAST alignment, the genomic sequences corresponding to the QTL regions were compared, and a TPR-like protein candidate gene was selected. Using whole-genome data, 138 candidate genes were found in four sequence fragments of two SSR markers located in the same scaffold. The genetic map and QTLs established in this study will aid in developing selective markers for agronomic traits and identifying corresponding genes, thereby providing a scientific basis for the further gene mapping of quantitative traits and the marker-assisted selection of functional genes in G. incarnatum breeding programs.
Lu-Lu Zhang,Yi-Yang Li,Jiang Hu,Guan-Qun Zhou,Lei Chen,Wen-Fei Li,Ai-Hua Lin,Jun Ma,Zhen-Yu Qi,Ying Sun 대한암학회 2018 Cancer Research and Treatment Vol.50 No.4
Purpose Local relapse-free survival (LRFS) differs widely among patients with T4 category nasopharyngeal carcinoma (NPC). We aimed to build a nomogram incorporating clinicopathological information to predict LRFS in T4 NPC after definitive intensity-modulated radiation therapy (IMRT). Materials and Methods Retrospective study of 415 Chinese patients with non-metastatic T4 NPC treated with definitive IMRT with or without chemotherapy at our cancer center between October 2009 and September 2013. The nomogram for LRFS at 3 and 5 years was generated based on multivariate Cox proportional hazards regression, and validated using bootstrap resampling, assessing discriminative performance using the concordance index (C-index) and determining calibration ability via calibration curves. Results Five-year LRFS was 88.8%. We identified and incorporated four independent prognostic factors for LRFS: ethmoid sinus invasion, primary gross tumor volume, age, and pretreatment body mass index. The C-index of the nomogram for local recurrence was 0.732 (95% confidence interval, 0.726 to 0.738), indicating excellent predictive accuracy. The calibration curve revealed excellent agreement between nomogram-predicted and observed LRFS probabilities. Risk subgroups based on total point score cutoff values enabled effective discrimination of LRFS. Conclusion This pretreatment nomogram enables clinicians to accurately predict LRFS in T4 NPC after definitive IMRT, and could help to facilitate personalized patient counselling and treatment strategies.
Neuropeptide Y Promotes the Treatment of Adipose Stem Cells on Type 2 Diabetic Wounds
Lu Tinghuan,Cong Lu,Jiang Tong,Dong Xiao,Song Lili 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.5
BACKGROUND: Type 2 diabetes (T2D) is a common metabolic disorder. Due to insufficient insulin secretion or insulin resistance, increased blood glucose often leads to impaired wound healing in T2D patients. Our previous research showed that adipose-derived stem cells (ASCs) from normal mice and T2D mice improved the cutaneous wound healing of diabetic mice. We also found that the expression of neuropeptide Y (NPY) in T2D ASCs was significantly decreased. METHODS: In order to explore the effects of NPY on ASCs and diabetic wound healing, we investigated the effects of NPY on ASCs proliferation and growth factors expression and secretion, the effects of NPY on skin fibroblasts, and the effects of NPY combined with ASCs on T2D wound healing. RESULTS: The results showed that a certain concentration of NPY could promote the proliferation and the growth factors expression and secretion of ASCs, and promote the proliferation and migration of fibroblasts. At the same time, NPY and ASCs have a synergistic effect, which can promote wound healing and decrease inflammation in T2D wounds. NPY may regulate ASCs through the ERK pathway. These results are conducive to promoting ASCs and NPY in the treatment of diabetic wounds. CONCLUSIONS: NPY can promote the effect of ASCs in the treatment of diabetic wounds.
Minjia Lu,Pingping Jiang,Pingbo Zhang,Shan Feng,Zhixuan Cui,Qingkui Song,Yong Zhao,Jingxian Shen,Yiyi Fan,Huixian Lu 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.128 No.-
A novel multifunctional plasticizer, cyclohexyl triethylene glycol oligo-lactate (CTLE) was designed by atwo-step esterification reaction using green non-toxic biomass lactic acid as raw material. Its structurewas characterized by 1H NMR, FT-IR and TOF-MS. Thermogravimetric analysis (TGA), tensile test andleaching test were used to evaluate the plasticizer effect of PVC or PLA blends. Compared with commercialplasticizers, CTLE showed more outstanding thermal stability, flexibility and migration stability. Under the same condition with the addition of 50 phr plasticizer, the CTLE plasticized PVC film hadthe lowest glass transition temperature (TgCTLE-50 = 24.6 C) and the highest initial decomposition temperature(TiCTLE-50 = 265.4 C). In PLA blends, only the CTLE plasticized PLA film could recover after fracture,and the stress relaxation test results showed that the stress value of PLA-CTLE-20 drops sharply to anon-zero value, showing elastic characteristics. We estimate that the excellent plasticity of CTLE is due toits molecular structure. Among them, a large number of polar groups form intermolecular forces withPVC chain segments to make the binding tight, and the ring structure increases the free volume betweenPVC chain segments.
Removal characteristics of Cd(II) ions from aqueous solution on ordered mesoporous carbon
Linhang Lu,Haibo Zhao,Lu Yan,Guowei Wang,Yulin Mao,Xin Wang,Kai Liu,Xiufang Liu,Qian Zhao,Tingshun Jiang 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.10
Ordered mesoporous carbon (CMK-3) was synthesized using SBA-15 mesoporous molecular sieve as a template and sucrose as carbon source. The materials were characterized by XRD, TEM and N2 physical adsorption technique. The resulting CMK-3 was used as adsorbent to remove Cd(II) ions from aqueous solution. The effect of pH, contact time and temperature on adsorption process was investigated in batch experiments. The results showed that the removal percentage could reach ca. 90% at the conditions of initial Cd(II) ions concentration of 20 mg/L, dose of 20mg, pH 6.5, contact time of 3h and 293K. Langmuir and Freundlich models were employed to describe the adsorption equilibrium. The kinetics data were described by the pseudo-first-order and pseudo-second-order models, respectively. The adsorption isotherm was well fitted to the Langmuir model, and the adsorption process was well described by the pseudo-second-order kinetic model.