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Won, Ga-Yeon,Moon, Bo-Mi,Oh, In-Gyeong,Matsuda, Kiku,Chaudhari, Atul A.,Hur, Jin,Eo, Seong-Kug,Yu, Il-Jeoung,Lee, Young-Ju,Lee, Yun-Sik,Kim, Byeong-Su,Lee, John Hwa Japan Poultry Science Association 2009 Journal of Poultry Science Vol.46 No.3
<P>The colibacillosis caused by avian pathogenic <I>E. coli</I> (APEC) is responsible for a significant loss of productivity and mortality in the poultry industry. The pathogenicity of these bacteria is based on the presence and expression of various virulence factors. In this study, the presence of the 19 virulence-associated genes in APEC was determined using PCR. Among the 118 <I>E. coli</I> isolates from the chickens with colibacillosis, all contained at least one of the 19 genes as approximately 95% of the isolates contained <I>fimC.</I> Interestingly, the <I>clpG</I> gene, which has not been detected in APEC previously, was detected in half of the isolates. The ColV plasmid-associated genes such as <I>colV, tsh, iucC, iucD and iss</I> genes were also detected in 57.6, 55.9, 50.0, 47.5, 47.5 and 41.5% of isolates, respectively. With regard to the fimbrial genes, the <I>papA</I> (14.4%), papC (14.4%) and <I>papG</I> genes (15.2%) were identified at relatively low rates, none of the isolates harbored <I>afa8D, f17A</I> or <I>facA</I>, and only 3 of the isolates (2.5%) contained <I>eaeA.</I> In this study, 94 isolates harbored two or more of the genes, and there were 43 different patterns of gene combination in the isolates. The most common pattern, which was found in 14.4% (17 isolates), was <I>clpG-fimC-iutA-colV-tsh-iucC-iucD-irp2-fyuA-vat-iss.</I> Overall, these results suggest that APEC strains in this area commonly contain multiple virulence factors and approximately half of the APEC strains contained the ColV plasmid-associated genes. Especially, <I>colV</I> and <I>tsh</I> were detected more than half of the isoaltes.</P>
Jeoung, Hye-Young,Song, Dae-Sub,Jeong, Woo Seog,Lee, Won-Ha,Song, Jae-Young,An, Dong-Jun The Society ; Maruzen Co. [distributor] 2013 The Journal of veterinary medical science Vol.75 No.1
<P>A multiplex reverse transcription polymerase chain reaction (mRT-PCR) assay was developed for the simultaneous detection of canine distemper virus (CDV), canine respiratory coronavirus (CRCoV) and canine influenza virus (CIV). These viral pathogens are all causative agents of canine infectious respiratory disease (CIRD). The sensitivity and specificity of the mRT-PCR were determined by comparing it to a rapid antigen test (RAT) or immuno-chromatography test kit and reverse transcription-polymerase chain reaction (RT-PCR) in the detection of CDV, CRCoV and CIV antigens present in 100 clinical samples (nasal swabs and whole blood samples) from 50 dogs with respiratory disease symptoms. This study revealed that mRT-PCR had almost exactly the same performance or results were almost 100% in agreement with that of RT-PCR and RAT both in terms of the assay sensitivity and specificity which was more highly evident in detecting CIV, CDV and CRCoV antigens present in canine nasal swab samples. Therefore, this assay could be a better alternative for the definitive and simultaneous ante-mortem detection of the three viral pathogens that cause CIRD by using nasal swabs.</P>
Development and clinical evaluation of a rapid diagnostic kit for feline leukemia virus infection
Won-Shik Kim,Chom-Kyu Chong,김학용,Gyu-Cheol Lee,정우석,Dong-Jun An,Hye-Young Jeoung,이재인,Young-Ki Lee 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.1
Feline leukemia virus (FeLV) causes a range of neoplasticand degenerative diseases in cats. To obtain a more sensitiveand convenient diagnosis of the disease, we preparedmonoclonal antibodies specific for the FeLV p27 to develop arapid diagnostic test with enhanced sensitivity andspecificity. Among these antibodies, we identified two clones(hybridomas 8F8B5 and 8G7D1) that specifically bound toFeLV and were very suitable for a diagnostic kit. The affinityconstants for 8F8B5 and 8G7D1 were 0.35 × 109 and 0.86 ×109, respectively. To investigate the diagnostic abilities of therapid kit using these antibodies, we performed severalclinical studies. Assessment of analytical sensitivity revealedthat the detection threshold of the rapid diagnostic test was 2ng/mL for recombinant p27 and 12.5 × 104 IU/mL for FeLV. When evaluating 252 cat sera samples, the kit was found tohave a kappa value of 0.88 compared to polymerase chainreaction (PCR), indicating a significant correlation betweendata from the rapid diagnostic test and PCR. Sensitivity andspecificity of the kit were 95.2% (20/21) and 98.5% (257/261),respectively. Our results demonstrated that the rapiddiagnostic test would be a suitable diagnostic tool for therapid detection of FeLV infection in cats.
Seroprevalence of subtype H3 influenza A virus in South Korean cats
Jeoung, Hye-Young,Shin, Bo-Hye,Lee, Won-Ha,Song, Dae-Sub,Choi, Young-Ki,Jeong, WooSeog,Song, Jae-Young,An, Dong-Jun SAGE Publications 2012 JOURNAL OF FELINE MEDICINE AND SURGERY Vol.14 No.10
<P>To investigate the potential transmission of subtype H3 influenza virus to cats, a serological survey was carried out in South Korea. Serum samples (n=1027) were obtained from 809 pet cats and 218 domesticated cats living in urban colonies (D-cats) from 2008 to 2010, and tested using an influenza anti-nucleoprotein (NP)-specific enzyme-linked immunosorbent assay (ELISA) and the haemagglutination inhibition (HI) test, which was recommended by the World Organization for Animal Health. Anti-influenza virus antibodies were detected in 3.12% and 2.43% of cat sera tested using the NP-specific ELISA and HI test, respectively. Anti-H3 antibodies were also identified when the HI assay was used for influenza virus serotyping. These data may indicate the sporadic transmission of subtype H3 influenza virus from other infected species to cats in South Korea.</P>
Jeoung, Hye-Young,Lee, Won-Ha,Jeong, Wooseog,Ko, Young-Joon,Choi, Cheong-Up,An, Dong-Jun Elsevier 2010 Research in veterinary science Vol.89 No.2
<P><B>Abstract</B></P><P>Virus-like particles (VLPs) are particles that consist of viral capsid proteins and are structurally similar to authentic virus. To express VLPs of the porcine encephalomyocarditis virus (EMCV) and investigate their efficacy and immuno response <I>in vivo</I>, a plasmid (P12A3C-pCI) containing the P12A and 3C genes of the EMCV-K3 viral strain was constructed. The VLPs of EMCV-K3 were successfully assembled in 293FT cells on 3 days after transfection with P12A3C-pCI and were identified as particles of about 30–40nm using transmission electron microscopy (TEM). In an <I>in vivo</I> experiment, the murine cytokines induced by VLPs of naked DNA vaccine showed that the Th1 indicators IL-2, TNF-α and GM-CSF, and the Th2 indicators IL-4 and IL-10 were increased. The immunization of mice with the P12A3C-pCI plasmid induced high levels of neutralizing antibody from 128- to 256-fold and led to a significant protection ratio (90%) after challenge with EMCV-K3 (wild-type strain). These VLPs may represent a novel vaccine strategy for the control of EMCV infection on pig farms.</P>
Lee, Eun,Oh, Seak-Hee,Kwon, Ji-Won,Kim, Byoung-Ju,Yu, Jin-Ho,Park, Chan-Jeoung,Hong, Soo-Jong The Korean Pediatric Society 2010 Clinical and Experimental Pediatrics (CEP) Vol.53 No.6
Chronic granulomatous disease (CGD) is an uncommon inherited disorder caused by mutations in any of the genes encoding subunits of the superoxide-generating phagocyte NADPH oxidase system, which is essential for killing catalase producing bacteria and fungi, such as $Aspergillus$ species, $Staphylococcus$ $aureus$, $Serratia$ $marcescens$, $Nocardia$ species and $Burkholderia$ $cepacia$. In case of a history of recurrent or persistent infections, immune deficiency should be investigated. Particularly, in the case of uncommon infections such as aspergillosis in early life, CGD should be considered. We describe here a case of CGD that presented with invasive pulmonary aspergillosis in a 2-month-old girl. We confirmed pulmonary aspergillosis noninvasively through a positive result from the culture of bronchial alveolar lavage fluid, positive serological test for $Aspergillus$ antigen and radiology results. She was successfully treated with Amphotericin B and recombinant IFN-${\gamma}$ initially. Six weeks later after discharge, she was readmitted for pneumonia. Since there were infiltrates on the right lower lung, which were considered as residual lesions, voriconazole therapy was initiated. She showed a favorable response to the treatment and follow-up CT showed regression of the pulmonary infiltrates.
Lee, Ho Won,Singh, Thoudam Debraj,Lee, Sang‐,Woo,Ha, Jeoung‐,Hee,Rehemtulla, Alnawaz,Ahn, Byeong‐,Cheol,Jeon, Young Hyun,Lee, Jaetae Federation of American Society for Experimental Bi 2014 The FASEB Journal Vol.28 No.7
Natural killer (NK) cell-based immunotherapy is a promising strategy for cancer treatment, and caspase-3 is an important effector molecule in NK cell-mediated apoptosis in cancers. Here, we evaluated the antitumor effects of NK cell-based immunotherapy by serial noninvasive imaging of apoptosis using a caspase-3 sensor in mice with human glioma xenografts. Human glioma cells expressing both a caspase-3 sensor as a surrogate marker for caspase-3 activation and Renilla luciferase (Rluc) as a surrogate marker for cell viability were established and referred to as D54-CR cells. Human NK92 cells were used as effector cells. Treatment with NK92 cells resulted in a time-and effector number-dependent increase in bioluminescence imaging (BLI) activity of the caspase-3 sensor in D54-CR cells in vitro. Caspase-3 activation by NK92 treatment was blocked by Z-VAD treatment in D54-CR cells. Transfusion of NK92 cells induced an increase of the BLI signal by caspase-3 activation in a dose-and time-dependent manner in D54-CR tumor-bearing mice but not in PBS-treated mice. Accordingly, sequential BLI with the Rluc reporter gene revealed marked retardation of tumor growth in the NK92-treatment group but not in the PBS-treatment group. These data suggest that noninvasive imaging of apoptosis with a caspase-3 sensor can be used as an effective tool for evaluation of therapeutic efficacy as well as for optimization of NK cell-based immunotherapy.
Won, Ok Jae,Lee, Jeung Joo,Eom, Min Yong,Suh, Su Jeoung,Park, Su Hyuk,Hwang, Ki Seon,Pyon, Jong Yeong,Park, Kee Woong The Korean Society of Weed ScienceThe Turfgrass So 2014 Weed & Turfgrass Science Vol.3 No.2
The continuous use of acetolactate synthase (ALS) and acetyl-CoA carboxylase (ACCase) inhibitors has led to the selection of herbicide resistant barnyardgrass populations in direct-seeded rice fields of Korea. This study was conducted to identify herbicide resistant barnyardgrass biotypes and to determine the cross- and multiple-resistance of them. 25% of the population collected from Taeahn was partially resistant to ACCase inhibitors and 22% collected from Kimjae were partially resistant to ALS inhibitors. However, 8.2% of the population from both sites was resistant to ALS and ACCase inhibitors. Resistance to sulfonylurea herbicide, flazasulfuron was identified from two barnyardgrass accessions collected from both Taeahn and Kimjae. One barnyardgrass accession from both sites was resistant to ACCase inhibitor, sethoxydim. The cross-resistance to ALS inhibitors was identified at one barnyardgrass accession from Taeahn and at two accessions from Kimjae. Further, crossresistance to ACCase inhibitors was also identified at barnyardgrass accessions from Taeahn and Kimjae. Multiple-resistance to flazasulfuron and sethoxydim was determined at four barnyardgrass accessions from Taeahn and at six accessions from Kimjae. Therefore, the herbicide mixture and sequences within a growing season or the herbicide rotation with different modes of actions across growing seasons are recommended to control herbicide-resistant barnyardgrass in infested fields.