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Effects of Traditional Acupuncture on Colonic Motility in the Rat with Colitis
Hee-YoungKim,In-SopShim,Hye-JungLee,SeongMokJeong,SunYoungKim,Tchi-ChouNam 한국임상수의학회 2003 한국임상수의학회지 Vol.20 No.1
The aims of this study were to investigate the efficacy of acupuncture on myoelectrical activity of inflamed or normal colon in the rat, and whether the effect of acupuncture on colonic motility was related to endogenous opioids. Twenty-two male Sprague-Dawley rats were divided into three groups. Experimental groups were normal group (n=8), colitis group (n=6) and naloxone group (n=8). Stainless steel bipolar electrodes were implanted on the serosal layer of the proximal colon of rats. Colitis was induced 7 days after electrode implantation using trinitrobenzene sulphonic acid (TNBS) and ethanol. Electromyograms (EMG) were recorded by using polygraph 11 days after implantation of electrodes. In normal group, normal colonic motility was recorded for 60min, and then traditional acupuncture at GV-1 was applied for 20min and EMG was recorded for further 60min in untreated rats. In colitis group, after recording of basal colonic motility for 60min, 20min of acupuncture treatment and further EMG recording was performed for 60min in TNBS/ethanol treated rats. In naloxone group, following subcutaneous administration of naloxone (3mg/kg), recording of EMG and acupuncture treatment were performed in TNBS/ethanol treated rats. In the normal group, acupuncture at GV-1 did not induce significant changes in colonic motility. TNBS/ethanol treatment had no significant effect on the frequency of colonic motility. And in colitis group, GV-1 acupuncture significantly decreased colonic motility (p<0.01). In naloxone group, after injection of naloxone, acupuncture at GV-1 did not change colonic motility in TNBS/ethanol treated rats. On the inflamed colon, naloxone blocked the effect of acupuncture. The present results suggested that endogenous opioids released by acupuncture at GV-1 decrease the motility of inflamed colon in rats, but not normal colon.
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Krill-Derived Phosphatidylserine Improves TMT-Induced Memory Impairment in the Rat
( Hyun Soo Shim ),( Hyun Jung Park ),( Yong Ho Ahn ),( Song Her ),( Jeong Jun Han ),( Dae Hyun Hahm ),( Hye Junglee ),( In Sop Shim ) 한국응용약물학회 2012 Biomolecules & Therapeutics(구 응용약물학회지) Vol.20 No.2
The present study examined the effects of krill-derived phosphatidylserine (Krill-PS) on the learning and memory function and the neural activity in rats with trimethyltin (TMT)-induced memory defl cits. The rats were administered vehicle (medium-chain triglyceride: MCT) or Krill-PS (50, 100 mg/kg, p.o.) daily for 21 days. The cognitive improving effl cacy of Krill-PS in TMT-induced amnesic rats was investigated by assessing the Morris water maze test and by performing choline acetyltransferase (ChAT), acetylcholinesterase (AChE) and cAMP responsive element binding protein (CREB) immunohistochemistry. The rats with TMT injection showed impaired learning and memory of the tasks and treatment with Krill-PS produced a signifl cant improvement of the escape latency to fl nd the platform in the Morris water maze at the 2nd and 4th day compared to that of the MCT group (p<0.05). In the retention test, the Krill-PS+MCT groups showed increased time spent around the platform compared to that of the MCT group. Consistent with the behavioral data, Krill-PS 50+MCT group signifl cantly alleviated the loss of acetylcholinergic neurons in the hippocampus and medial septum compared to that of the MCT group. Treatment with Krill-PS signifl cantly increased the CREB positive neurons in the hippocampal CA1 area as compared to that of the MCT group. These results suggest that Krill-PS may be useful for improving the cognitive function via regulation of cholinergic marker enzyme activity and neural activity.
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김상국,이상철,정형진,Bong-HoLee,In-JungLee 한국작물학회 2003 Korean journal of crop science Vol.48 No.4
For storage periods of tubers in Chinese yam, the levels of GA/sub 44/ and GA/sub 20/ was constant, meanwhile both GA/sub 53/ and GA/sub l9/ level were always higher than that of GA/sub 44/ and GA/sub 20/ㆍ GA/sub 9/ content as precursor of GA₄ was not changed during storage. GA/sub 24/ content was low to below 0.2 ng for 90 days after storage, GA/sub 36/ content as precursor of GA₄ like GA/sub 9/ was about 6-8 fold higher than that of GA/sub 9/ during storage. GA contents of the two gibberellin biosynthetic pathways were gradually increased when storage periods were progressed. Bioactive GA₁ content as the GA members of an early C-13 hydroxylation was always constant, and its content was very low as below 0.1ng per dry weight, meanwhile, bioactive GA₄ content as the GA members of non C-13 hydroxylation was drastically increased, also, its content was highest at 90 days after storage, and then decreased at 120 days after storage. Consequently, we suggest that GA₄ may be involved in controlling tuber sprouting in Chinese yam.
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