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고병섭,주혜정,마진열,박갑주,안상우 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1
In order to improve the qualities of boiling extract the availability of standard compounds was investigated in extract of Scutellariae Radix. The standard compounds used baicalin(1), baicalein(2), and wogonin(3). The availabilities were analyzed by thin layer chromatography(TLC) and high performance liquid chromatography(HPLC) on C18 column. The extract of RS-4(W/V=10g/100ml) showed the highest availabilities as 7.95% of baicalin(1), 1.04% of baicalein(2), and 0.31% of wogonin(3).
정갑수,고신관 서울보건대학 1998 서울보건대학 부설 한국보건과학연구소 논문집 Vol.5 No.1
Image-based three dimensional radiation treatment planning(3D RTP) has a potential of generating superior treatment plans. Advances in computer technology and software developments quickly make 3D RTP a feasible choice for routine clinical use. However, it has become clear that evaluation of a 3D plan is more difficult than a 2D plan. A number of tools has been developed to facilitate evaluation of 3D RTP both qualitatively and quantitatively. For example, beam's eye view(BEV) is one of the most powerful and time-saving method as a qualitative tool. Dose-volume histogram(DVH) has been proven to be one of the most valuable method for a quantitative tool. But it has a limitation to evaluate several different plans for biological effects of the tissue and critical organ. Therefore. there is a strong interest in developing quantitative models which would predict the likely biological response of irradiated organs and tissues. such as tumor control probability(TCP) and normal tissue complication probability(NTCP). DVH and NTCP of hepatoma were evaluated for three dimensional conformal radiotherapy(3D CRT). Also, 3D RTP was analysed as a dose optimization based on beam arrangement and beam modulation. The value of NTCP in patients with hepatitis was higher than that in patients without hepatitis. Also the value of n =0.32(volume factor) was highly correlated to prediction of radiation hepatitis. Therefore, we concluded that the possibility of radiation hepatitis was highly suggested if NTCP was over 40%.
한약 탕제분획의 항 Herpes simplex virus 활성에 관한 연구
강봉주,고병섭,양기상,박갑주 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1
Herpes simplex viruses(HSV) are one of the most common infectious virus of man. Though chemotherapies and antiviotics against HSV have been developed in many countries, but anti-HSV agents were not satisfactory to mankind by their toxic reaction and side effects. In order to search for anti-HSV agents from Korean traditional prescriptions, we extended the number of specimens. Both methanol extract and boiling water extract of the Korean traditional prescriptions were screened to detect anti-HSV activities by MTT assay. Korean traditional prescriptions showing anti-HSV activities as methanol extracts were Paekyo˘psan, Chesu˘pwilyu˘ngtang, Yongdamsangantang, and prescription 11. Four methanol extracts showing anti- HSV activities were fractionated by hexane and their efficacies were tested. Hexane fractionations of Paekyo˘psan, Chesu˘pwilyu˘ngtang, and prescription 11 showed in anti-HSV in tissue culture and their selectivity indexes (SI) were 19.50, 3.32 and 42.90, respectively. Methanol fractionation of Paekyo˘psan showed anti-HSV activities and its SI was 1.40. Especially Paekyo˘psan showed anti-HSV activities both haxane and methanol fractionation.
In situ tissue regeneration through host stem cell recruitment
In Kap Ko,Sang Jin Lee,Anthony Atala,James J. Yoo 생화학분자생물학회 2013 Experimental and molecular medicine Vol.45 No.s
The field of tissue engineering has made steady progress in translating various tissue applications. Although the classical tissue engineering strategy, which involves the use of culture-expanded cells and scaffolds to produce a tissue construct for implantation, has been validated, this approach involves extensive cell expansion steps, requiring a lot of time and laborious effort before implantation. To bypass this ex vivo process, a new approach has been introduced. In situ tissue regeneration utilizes the body’s own regenerating capacity by mobilizing host endogenous stem cells or tissue-specific progenitor cells to the site of injury. This approach relies on development of a target-specific biomaterial scaffolding system that can effectively control the host microenvironment and mobilize host stem/progenitor cells to target tissues. An appropriate microenvironment provided by implanted scaffolds would facilitate recruitment of host cells that can be guided to regenerating structural and functional tissues.