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Isolation and Characterization of Panax ginseng 14-3-3 Gene Family
( Hwa Jee Chung ),( Joo Young Park ),( In Sook Cho ),( Cheol Goo Hur ),( Dong Woog Choi ),( Jang Ryol Liu ) 한국식물학회 2004 Journal of Plant Biology Vol.47 No.3
14-3-3 proteins are a family of conserved proteins expressed in all eukaryotes. They function as regulators in signaling pathways through protein-protein interaction. These proteins have been implicated in plant metabolism, development, and responses to abiotic and biotic stresses. Here, we isolated six cDNAs encoding 14-3-3 proteins of Panax ginseng, designated as Pg14-3-3, by searching the ginseng EST database. Their degree of amino acid identity ranged from 53 to 84%. Phylogenetic analysis showed that the Pg14-3-3 isoforms can be divided into two groups by early duplication, with one group being further split into three subgroups because of subsequent duplication events. Pg14-3-3s are differentially expressed in various root tissues and leaves, and their transcript levels are up- or down-regulated by either cold.or drought stresses. These results suggest a possible role for Pg14-3-3 proteins in abiotic stress responses.
Chung, Hwa-Jee,Cho, In Sook,Kim, Jee-Hyub,In Dong Su,Hur, Cheol Goo,Song, Ji Sook,Woo, Sung Sick,Choi, Dong-Woong,Liu, Jang Ryol 한국식물학회 2003 Journal of Plant Biology Vol.46 No.3
Researchers have widely adopted the hairy mot culture system as a means for producing secondary metabolites, including ginsenosides from ginseng. Although bacterial genes are involved, the aspects of plant gene expression are unclear. Using a cDNA microarray approach, we identified genes that are differentially expressed in ginseng hairy roots after Agrobacterium rhizogenes infection. Our goal was to gain an initial understanding of the correlation between hairy root morphology and ginsenoside production. Among the 250 genes analyzed here, 63 (including 14 that are unclassified) were differentially expressed in a hairy root line containing a high level of ginsenosides. Of the genes that had been functionally categorized, 29% and 17% were active in metabolism and stress responses, respectively. Most were primarily associated with ribosomal proteins, thereby functioning in protein synthesis and destination. Their expression was down-regulated in hairy roots having less lateral branching. This phenotype may have resulted from the manipulation of metabolic activities by the translational machinery.
Chung, Jong-Kyun,Jee, Geon-Hwa,Lee, Chi-Na 한국우주과학회 2011 Journal of Astronomy and Space Sciences Vol.28 No.4
The total electron content (TEC) using global positioning system (GPS) is analyzed to see the characteristics of ionosphere over King Sejong station (KSJ, geographic latitude 62°13′ S, longitude 58° 47′ W, corrected geomagnetic latitude 48° S) in Antarctic. The GPS operational ratio during the observational period between 2005 and 2009 is 90.1%. The annual variation of the daily mean TEC decreases from January 2005 to February 2009, but increase from the June 2009. In summer (December-February), the seasonal mean TEC values have the maximum of 26.2 ± 2.4 TEC unit (TECU) in 2005 and the minimum of 16.5 ± 2.8 TECU in 2009, and the annual differences decrease from 3.0 TECU (2005-2006) to 1.4 TECU (2008-2009). However, on November 2010, it significantly increases to 22.3 ± 2.8 TECU which is up to 5.8 TECU compared with 2009 in summer. In winter (June-August), the seasonal mean TEC slightly decreases from 13.7 ± 4.5 TECU in 2005 to 8.9 ± 0.6 TECU in 2008, and the annual difference is constantly about 1.6 TECU, and increases to 10.3 ± 1.8 TECU in 2009. The annual variations of diurnal amplitude show the seasonal features that are scattered in summer and the enhancements near equinoxes are apparent in the whole years. In contrast, the semidiurnal amplitudes show the disturbed annual peaks in winter and its enhancements near equinoxes are unapparent. The diurnal phases are not constant in winter and show near 12 local time (LT). The semidiurnal phases have a seasonal pattern between 00 LT and 06 LT. Consequently, the KSJ GPS TEC variations show the significant semidiurnal variation in summer from December to February under the solar minimum between 2005 and 2009. The feature is considered as the Weddell Sea anomaly of larger nighttime electron density than a daytime electron density that has been observed around the Antarctica peninsula.
Plasmaspheric contribution to the GPS TEC
Jee, Geon-Hwa,Lee, Han-Byul,Kim, Yong-Ha,Chung, Jong-Kyun,Cho, Jung-Ho 한국우주과학회 2010 한국우주과학회보 Vol.19 No.1
We performed a comprehensive comparison between GPS Global Ionosphere Map (GIM) and TOPEX/Jason (T-J) TEC data for the periods of 1998~2009 in order to assess the performance of GIM over the global ocean where the GPS ground stations are very sparse. Using the GIM model constructed by CODE at University of Bern, the GIM TEC values were obtained along the T-J satellite orbit at the locations and times of the measurements and then binned into various geophysical conditions for direct comparison with the T-J TECs. On the whole, the GIM model was able to reproduce the spatial and temporal variations of the global ionosphere as well as the seasonal variations. However, the GIM model was not accurate enough to represent the well-known ionospheric structures such as the equatorial anomaly, the Weddell Sea Anomaly, and the longitudinal wave structure. Furthermore, there seems to be a fundamental limitation of the model showing the unexpected negative differences (i.e., GPS < T-J) in the northern high latitude and the southern middle and high latitude regions. The positive relative differences (i.e., GIM > T-J) at night represent the plasmaspheric contribution to GPS TEC, which is maximized, reaching up to 100% of the corresponding T-J TEC values in the early morning sector. In particular, the relative differences decreased with increasing solar activity and this may indicate that the plasmaspheric contribution to the maintenance of the nighttime ionosphere does not increase with solar activity, which is different from what we normally anticipate. Among these results, the plasmaspheric contribution to the ionospheric GPS TEC will be presented in this talk and the rest of it will presented in the companion paper (poster presentation).
( Jee Eun Yang ),( Eun Ji Park ),( Hyo Jeong Lee ),( Hyo Jeong Kang ),( Kang Mo Kim ),( Eun Sil Yu ),( Dan Bi Lee ),( Ju Hyun Shim ),( Young Suk Lim ),( Han Chu Lee ),( Young Hwa Chung ),( Yung Sang L 대한간학회 2014 Clinical and Molecular Hepatology(대한간학회지) Vol.20 No.1
Background/Aims: The role of prostaglandin E2 (PGE2) in the modulation of cell growth is well established in colorectal cancer. The aim of this study was to elucidate the significance of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) down-regulation on the prognosis of hepatocellular carcinoma (HCC) patients. Methods: The expression of 15-PGDH in HCC cell lines and resected HCC tissues was investigated, and the correlation between 15-PGDH expression and HCC cell-line proliferation and patient survival was explored. Results: The interleukin-1-β-induced suppression of 15-PGDH did not change the proliferation of PLC and Huh-7 cells in the MTS [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The induction of 15-PGDH by transfection in HepG2 cells without baseline 15-PGDH expression was suppressed at day 2 of proliferation compared with empty-vector transfection, but there was no difference at day 3. Among the 153 patients who received curative HCC resection between 2003 and 2004 at our institution, 15-PGDH expression was observed in resected HCC tissues in 56 (36.6%), but the 5-year survival rate did not differ from that of the remaining 97 non-15-PGDH-expressing patients (57.1% vs 59.8%; P=0.93). Among 50 patients who exhibited baseline 15-PGDH expression in adjacent nontumor liver tissues, 28 (56%) exhibited a reduction in 15-PGDH expression score in HCC tissues, and there was a trend toward fewer long-term survivors compared with the remaining 22 with the same or increment in their 15-PGDH expression score in HCC tissues. Conclusions: The prognostic significance of 15-PGDH down-regulation in HCC was not established in this study. However, maintenance of 15-PGDH expression could be a potential therapeutic target for a subgroup of HCC patients with baseline 15-PGDH expression in adjacent nontumor liver tissue. (Clin Mol Hepatol 2014;20:28-37)
증례 : 소화기 ; 시스플라틴을 이용한 경동맥 화학색전술 후 발생한 용혈성 요독증후군 1예
양지은 ( Jee Eun Yang ),김강모 ( Kang Mo Kim ),심주현 ( Ju Hyun Shim ),임영석 ( Young Suk Lim ),이한주 ( Han Chu Lee ),정영화 ( Young Hwa Chung ),이영상 ( Yung Sang Leef ) 대한내과학회 2013 대한내과학회지 Vol.85 No.1
Hemolytic uremic syndrome (HUS) is a rare disorder characterized by microangiopathic hemolytic anemia, thrombocytopenia, and acute renal failure. These characteristics are related to the formation of platelet-rich microthrombi in the microvasculature. HUS is associated with a variety of etiologies, including cisplatin. Previously reported HUS cases after cisplatin administration were almost always related to systemic combination chemotherapy that included cisplatin. Transarterial chemoembolization (TACE) is commonly used in treatment of hepatocellular carcinoma, and most centers in Korea use cisplatin. A 70-year-old female with hepatocellular carcinoma was treated with TACE including cisplatin and subsequently developed clinical and laboratory findings compatible with HUS. (Korean J Med 2013;85:67-72)