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Isolation of a Calmodulin-binding Transcription Factor from Rice (Oryza sativa L.)
Choi, Man-Soo,Kim, Min-Chul,Yoo, Jae-Hyuk,Moon, Byeong-Cheol,Koo, Sung-Cheo,Park, Byung-Ouk,Lee, Ju-Huck,Koo, Yoon-Duck,Han, Hay-Ju,Lee, Sang-Yeol,Chung, Woo-Sik,Lim, Chae-Oh,,Cho, Moo-Je Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.
Calmodulin (CaM) regulates diverse cellular functions by modulating the activities of a variety of enzymes and proteins. However, direct modulation of transcription factors by CaM has been poorly understood. In this study, we isolated a putative transcription factor by screening a rice cDNA expression library by using CaM:horseradish peroxidase as a probe. This factor, which we have designated OsCBT (Oryza sativa CaM-binding transcription factor), has structural features similar to Arabidopsis AtSRs/AtCAMTAs and encodes a 103-kDa protein because it contains a CG-1 homology DNA-binding domain, three ankyrin repeats, a putative transcriptional activation domain, and five putative CaM-binding motifs. By using a gel overlay assay, gel mobility shift assays, and site-directed mutagenesis, we showed that OsCBT has two different types of functional CaM-binding domains, an IQ motif, and a Ca^(2+)-dependent motif. To determine the DNA binding specificity of OsCBT, we employed a random binding site selection method. This analysis showed that OsCBT preferentially binds to the sequence 5'-TWCG(C/T)GTKKKKTKCG-3' (W and K represent A or C and T or G, respectively). OsCBT was able to bind this sequence and activate β-glucuronidase reporter gene expression driven by a minimal promoter containing tandem repeats of these sequences in Arabidopsis leaf protoplasts. Green fluorescent protein fusions of two putative nuclear localization signals of OsCBT, a bipartite and a SV40 type, were predominantly localized in the nucleus. Most interestingly, the transcriptional activation mediated by OsCBT was inhibited by co-transfection with a CaM gene. Taken together, our results suggest that OsCBT is a transcription activator modulated by CaM.
남성 공황장애 환자에서의 monoamine oxidase A 프로모터 유전자 다형성의 연합 연구
최영희,우종민,박헌구,조우연,윤혜영,윤경식,조대연,홍경수 白中央醫療院 2004 仁濟醫學 Vol.25 No.1
Objectives: Genetic variation of the monoamine oxidase (MAO) A promoter gene polymorphism has been associated with its functional capacity. The authors tried to find the association between MAO A promoter polymorphism and panic disorder. Methods: 317 patients with panic disorder and 92 normal controls were enrolled in this study. 308 patients and 84 normal controls were completed study. Results: There was significant difference in allele frequencies of MAO A promoter polymorphism between male panic patients and normal controls (s allele: 60.7% vs. 44.3%, 1 allele: 39.3% vs 55.7%, x^(2)=10.008, df=1, p=0.002). The frequencies of MAO A promoter alleles between panic disorder and normal controls were 2 repeat (1.1% vs. 1.2%), 3 repeat (58.3% vs. 45.8%), 4 repeat (40.6% vs. 53.0%)(x2=8.368, df=2, p=0.015). Conclusion: Our results suggest that MAO A promoter gene may be related to panic disorder.
Synthesis of catechol‐conjugated chitosan and its application as an additive for cement mortar
Choi Hoe Young,Bae Sung‐Ho,Choi Se‐Jin,Ko Haye Min 대한화학회 2022 Bulletin of the Korean Chemical Society Vol.43 No.1
We synthesized catechol-conjugated chitosan (CCC) to study its usefulness as a construction material additive in cement mortar. The degree of catechol conjugation (DOCcat) of the synthesized CCC was determined to be approximately 14% by UV–vis and 1H NMR spectroscopy. Furthermore, the hydroxyl and amine groups in CCC could play a crucial role in hydrogen bonding, metal coordination, and cross-linking processes via interaction with adducts from cement mortar. In this study, we observed an improvement in the compressive strength and absorption rate, suggesting that CCC is a promising candidate for high-performance cement mortar.
Ko, Haye Min,Choi, Hyunbong,Paek, Sanghyun,Kim, Kyungjun,Song, Kihyung,Lee, Jae Kwan,Ko, Jaejung Royal Society of Chemistry 2011 Journal of materials chemistry Vol.21 No.20
<P>The synthesis and characterization of new push-pull chromophores, bisDMFA-diTh-CA, bisDMFA-diTh-MMN, and bisDMFA-diTh-MIMN from 5′-(4-(bis(9,9-dimethyl-9<I>H</I>-fluoren-2-yl)aniline (bisDMFA) electron donating, dithiophene bridging, and various electron withdrawing moieties, cyanoacrylic acid (CA), methylene malononitrile (MMN), and methylene indenylidene malononitrile (MIMN), were demonstrated for efficient solution processed BHJ solar cells. The photophysical properties, the hole mobilities from the space charge limitation of current (SCLC) <I>J</I>-<I>V</I> characteristics, and surface morphologies of these materials/PCBM bulk-heterojunction films were also investigated. The best power conversion efficiency of 3.66% (±0.12) with <I>J</I><SUB>sc</SUB> = 11.82 mA/cm<SUP>2</SUP>, <I>FF</I> = 0.35, and <I>V</I><SUB>oc</SUB> = 0.90 V was observed in the BHJ film fabricated with the bisDMFA-diTh-MMN/C<SUB>71</SUB>-PCBM composite with 3% 1-chloronaphthalene (CN), which is an efficiency ∼82% and ∼49% higher than before and after post-annealing without treatment of CN, respectively.</P> <P>Graphic Abstract</P><P>New push-pull chromophores, bisDMFA-diTh-CA, bisDMFA-diTh-MMN, bisDMFA-diTh-MIMN were synthesized and a power conversion efficiency of 3.66% was observed in the BHJ film fabricated using 3% 1-chloronaphthalene. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1jm10667h'> </P>
H₂O-LiBr를 작동유체로 한 건물 냉난방용 열배터리 시뮬레이션 연구
민하예(Haye Min),최형원(Hyung Won Choi),강용태(Yong Tae Kang) 대한설비공학회 2021 대한설비공학회 학술발표대회논문집 Vol.2021 No.6
In this study, a simulation study of thermal battery for cooling and heating in buildings using H2O-LiBr was conducted. With the assumption of well-conditioned external fluids, a mathematical model is established. The characteristics such as pressure, temperature, concentration, and power change of the thermal battery system during charging and discharging were investigated, and the performance of the thermal battery system was analyzed. As a result, it was found that the COPs of cooling and heating were 0.594 and 0.960, respectively. This demonstrates that the H₂O-LiBr thermal battery system can be an effective means for the storage of thermal energy in building.
카테콜-키토산 하이드로겔의 대용량 합성과 자가 치유 특성 분석
최회영 ( Choi Hoe Young ),고혜민 ( Ko Haye Min ) 한국건축시공학회 2022 한국건축시공학회 학술발표대회 논문집 Vol.22 No.2
Chemical crosslinking is the most widely used method for hydrogel preparations. We prepared a hydrogel using chitosan catechol/polyvinyl alcohol and sodium tetraborate decahydrate (Na<sub>2</sub>B<sub>4</sub>O<sub>7</sub>·10H<sub>2</sub>O). The formation of hydrogels often presents inconsistent results and issues according to the reaction scale. Therefore, we measured and analyzed the self-healing property and viscoelasticity of hydrogels attributed to scale-up synthesis using a rheometer.
식이의 불포화 지방산/포화지방산 ( P/S ) 의 비가 2-Acetylaminofluorene ( 2-AAF )를 투여한 쥐 간의 지질과산화도 및 과산화물 대사효소에 미치는 영향
김경민,최혜미,임경숙 한국지질학회 1992 韓國脂質學會誌 Vol.2 No.1
The effect of dietary polyunsaturated fatty acid/saturated fatty acid (P/S) ratios on hepatic lipid peroxidation and peroxide metabolizing enzyme activities in 2-acetylaminofluoredne (2-AAF) treated rats have been compared in an effort to understand the possible mechanisms of dietary fats to promote tumorigenic process. Male Sprague-Dawley rats were fed the diet of P/S 0.08, 1.0 and 4.0 at the level of 15% fat (w/w). At 23th and 27th weeks, 2-AAF (50 ㎎ of 2-AAF/Kg body wt.) was injected twice by intraperitoneally and liver microsomal and cytosolic fractions were used to determine the lipid peroxide, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione S-transferase (GST) activities. While hepatic microsomal lipid peroxide levels and cytosolic GSH-Px elevated as dietary P/S ratio increases. cytosolic SOD and GST activities were not changed by dietary P/S ratios. By 2-AAF treatment SOD activity was significantly increased in P/S 0.08 group compared with higher P/S groups. These results indicate that higher P/S diet itself may serve as a promoter of hepatic lipid peroxidation and an inducer of GSH-Px, while lower P/S diet may enhance detoxification pathway through elevated SOD activity after treatment with 2-AAF. Therefore, the selective inductions of GSH-Px and SOD provide new insight into the role of those enzymes in the promotional effects of polyunsaturated fats in tumorigenesis.
Kim, Kyung-Min,Yim, Kyeong-Sook,Choi, Hay-Mie Korean Society of ToxicologyKorea Environmental Mu 1995 Toxicological Research Vol.11 No.2
This paper examines the effects of dietary polyunsaturated fatty acid/saturated fatty acid (p/s) ratios and butylated hydroxytoluene (BHT) on the hepatic microsomaI mixed-function oxidase sy. stem in 2~acetylaminofiuorene (2-AAF) treated rats. Sprague-Dawley male rats were fed the diet of beef tallow (p/s 0.08), beef tallow plus soybean oil (p/s 1.0), and soybean oil (p/s 4.0) at the level of 15%fat and with or without 0.3% BHT. After 2-AAF was injected twice at the ages of 23 and 27 weeks, cholesterol/phospholipid molar ratio, thiobarbituric acid reactive substances (TBARS) level, cytochrome P450, cytochrome $b_5$, NADPH-cytochrome $b_5$, and NADPH-cytochrome c reductase activity were measured from isolated hepatic microsomal fractions. In the beef tallow (p/s 0.08) and beef tallow plus soybean oil (p/s 1.0) groups, cholesterol/phospholipid molar ratio showed decreasing tendency by 2-AAF and BHT. Cytochrome P-450 content was decreased in the group of soybean oil (p/s 4.0) and NADPH-cytochrome c reductase activity was increased by 2-AAF and BHT in all the dietary groups. While TBARS levels were increased by 2-AAF in all the dietary groups, they were reduced by BHT in the soybean oil (p/s 4.0) group. These results suggest that long term intake of soybean oil (p/s 4.0) diet induced changes in the nature of microsomal membrane and induced less cytochrome P-450, low level feeding of BHT increased cytochrome c reductase activity and lowered microsomal lipid peroxidation levels, which were increased by 2-AAF treatment.
Seo, Jeong-Min,Park, Kyung-Ae,Yeo, Eui-Zu,Choi, Hay-Mie Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.3
This study was designed to examine the anticarcinogenic effect of dietary supplementation with garlic powder on rat hepatocarcinogenesis. All rats were initiated by a single dose (200 mg/body weight) intraperitoneal injection of diethylnitrosamine (DEN), and three weeks later, subjected to two-thirds partial hepatectomy. Two weeks after initiation, four groups of rats were given experimental diets supplemented with 0 (control group), 0.5, 2.0, or 5.0% garlic powder for 6 weeks. Rats were sacrificed at eight weeks after initiation. The induction of placental glutathione S-transferase (GST-P) positive foci was significantly inhibited almost equally in all three groups fed garlic diets. Glucose 6-phosphatase (G6Pase) activity was increased in rats fed 0.5% and 2.0% garlic powder, and was negatively correlated with the number and area of GST-P positive foci. Thiobarbituric acid reactive substance (TBARS) contents were decreased in rats fed 2.0% and 5.0% garlic powder. Only 5.0% garlic powder supplementation significantly increased the glutathione content and the glutathione S-transferase activity, compared to the control group. Therefore, all levels of garlic powder, 0.5% to 5.0%, exerted an anti promotional effect during hepatocarcinogenesis. Dietary supplementation with garlic powder seemed to maintain microsomal membrane integrity by increasing G6Pase activities. Glutathione-dependent detoxifying enzymes did not seem to contribute to this protective effect directly. The present study suggests that garlic powder is effective in inhibiting the induction of GST-P positive foci, possibly by stabilizing the hepatic microsomal membrane.