Isoliquiritigenin-mediated p62/SQSTM1 induction regulates apoptotic potential through attenuation of caspase-8 activation in colorectal cancer cells

Jin, Hao,Seo, Geom Seog,Lee, Sung Hee Elsevier 2018 european journal of pharmacology Vol.841 No.-

<P><B>Abstract</B></P> <P>Isoliquiritigenin (ISL) is a natural flavonoid that exhibits anticancer properties in various carcinoma cell types. However, the precise mechanism responsible for its anticancer activity has not been elucidated fully. In the present study, we examined ISL-mediated apoptotic mechanisms in colorectal cancer (CRC) cells. ISL induced apoptosis in human HCT-116 cells and caused marked induction of p62/SQSTM1 mRNA and protein expression. Similarly, ISL potently inhibited <I>in vivo</I> tumor growth and induced p62/SQSTM1 expression in xenograft tumor tissues. In a p62/SQSTM1 siRNA transfection study, ISL-induced p62/SQSTM1 expression attenuated ISL-mediated apoptosis by reducing caspase-8 activation. ISL potentiated the apoptotic effects of 5-fluorouracil (5-FU) on HCT-116 cells. However, ISL-induced p62/SQSTM1 expression also attenuated the potency of apoptosis induced by the combination of 5-FU and ISL. Our results demonstrate that ISL-induced p62/SQSTM1 upregulation affects ISL-mediated apoptotic potential through attenuation of caspase-8 activation in CRC cells. These findings broaden the understanding of the molecular basis of ISL-mediated apoptosis.</P>

Design and Implementation of a USN Middleware for Context-Aware and Sensor Stream Mining

Jin, Cheng-Hao,Lee, Yang-Koo,Lee, Seong-Ho,Yun, Un-il,Ryu, Keun-Ho Korea Spatial Information Society 2011 한국공간정보학회지 Vol.19 No.1

Recently, with the advances in sensor techniques and net work computing, Ubiquitous Sensor Network (USN) has been received a lot of attentions from various communities. The sensor nodes distributed in the sensor network tend to continuously generate a large amount of data, which is called stream data. Sensor stream data arrives in an online manner so that it is characterized as high-speed, real-time and unbounded and it requires fast data processing to get the up-to-date results. The data stream has many application domains such as traffic analysis, physical distribution, U-healthcare and so on. Therefore, there is an overwhelming need of a USN middleware for processing such online stream data to provide corresponding services to diverse applications. In this paper, we propose a novel USN middleware which can provide users both context-aware service and meaningful sequential patterns. Our proposed USN middleware is mainly focused on location based applications which use stream location data. We also show the implementation of our proposed USN middleware. By using the proposed USN middleware, we can save the developing cost of providing context aware services and stream sequential patterns mainly in location based applications.

Two Mechanisms of Packet Dropout Compensation for Linear Filter of Networked Control Systems

Hao Jin,Jing Ma 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.11

This paper studies the optimal linear filtering problem for networked control systems (NCSs) with multiple packet dropouts. When the current measurement is lost, the two popular compensation mechanisms (the hold-input and zero-input mechanism) are used for compensation, respectively. The hold-input strategy means that the latest measurements received is applied directly whereas the zero-input one adopts zero value. Based on the two popular compensators, the optimal linear filters in the linear minimum variance sense are given by innovation analysis approach, and their performances are compared in terms of two simulation examples. The conclusion is that neither of the two compensation mechanisms can be claimed to be superior to the other.

Comparative Study of DC Breakdown and Space Charge Characteristics of Insulation Paper Impregnated with Natural Ester and Mineral Oil

Hao, Jian,Zou, Run-Hao,Liao, Rui-Jin,Yang, Li-Jun,Liao, Qiang,Zhu, Meng-Zhao The Korean Institute of Electrical Engineers 2018 Journal of Electrical Engineering & Technology Vol.13 No.4

Natural ester is a suitable substitute for mineral oil and has been widely used in AC transformer in many countries. In order to further application of natural ester in direct current (DC) equipment, it is needed to investigate its long term insulation property under DC condition. In this paper, a thermal ageing experiment was conducted for both mineral oil-paper and natural ester-paper insulation. The DC breakdown and space charge characteristics of insulation paper impregnated with natural ester and mineral oil was compared. Results show that the resistivity of the paper immersed in natural ester and mineral oil both increase as the ageing goes on. While insulation paper impregnated with natural ester has higher resistivity and DC breakdown voltage than the paper impregnated with mineral oil. The DC breakdown voltage for the oil impregnated insulation paper being DC pre-stressing is higher than that without pre-stressing. The average DC breakdown field strength difference between the test with pre-stressing and without pre-stressing clearly shows that there is an apparent enhancement effect for the homo-charge injection on the DC breakdown.

Effects of selenium source and level on the physiological response, reproductive performance, serum Se level and milk composition in gestating sows

Jin, Xing Hao,Kim, Cheon Soo,Gim, Min Jin,Kim, Yoo Yong Asian Australasian Association of Animal Productio 2022 Animal Bioscience Vol.35 No.12

Objective: This study was conducted to evaluate the effects of selenium (Se) source and level on the physiological response, reproductive performance, serum Se level, and milk composition in gestating sows. Methods: A total of 54 multiparous sows (Yorkshire×Landrace) with average body weight (BW), backfat thickness (BF), and parity were assigned to one of five treatments with 10 or 11 sows per treatment using a 2×2 factorial arrangement with one additional treatment in a completely randomized design. Inorganic or organic Se (IS or OS) sources were added to the diet at 0.30 ppm and 0.50 ppm Se. A non-Se-fortified corn-soybean meal basal diet served as a negative control. Treatments were as follows: i) Control: corn-soybean based diet, ii) IS30: control+inorganic Se 0.30 ppm, iii) IS50: control+inorganic Se 0.50 ppm, iv) OS30: control+ organic Se ppm, and v) OS50: control+organic Se 0.50 ppm. Results: At day 21 of lactation, piglet weight and weight gain in the OS treatments were higher than those in the IS treatments (p<0.05). Meanwhile, adding 0.5 ppm Se also resulted in the same significant differences in piglet BW and weight gain (p<0.05). Colostrum and milk Se concentrations increased (p<0.05) with Se level for both Se sources but were greater when sows were fed organic Se (p<0.05). Except for 24 hours postpartum, the Se concentrations were higher when sows were fed organic Se (p<0.05). Sow serum Se content was greater as Se levels increased from 0.3 ppm to 0.5 ppm at day 110 of gestation, 24 hours postpartum and day 21 of lactation (p<0.05). The pig serum Se concentration increased as the dietary Se level increased (p<0.05) and was higher when the sow dietary Se source was organic (p<0.05). Organic Se 0.5 ppm also had the highest serum Se level at two measured points (p<0.05). Conclusion: Consequently, supplementation with organic Se or 0.5 ppm Se in a gestating diet could improve piglet performance, the Se status of sows and piglets and milk composition, but organic Se at 0.5 ppm is optimal.

Synthesis and characterization of cholic acid-containing biodegradable hydrogels by photoinduced copolymerization

Hao, Jin-Qing,Li, Hong,Woo, Hee-Gweon Wiley Subscription Services, Inc., A Wiley Company 2009 Journal of applied polymer science Vol.112 No.5

<P>A cholic acid (CA)-containing biodegradable hydrogel (PLA-PEG-PLA-co-MACAH) was synthesized from the photoinduced copolymerization of a CA-modified methacrylate monomer (MACAH), bearing a spacer of hexane-1,6-diol spacer between the methacryloyl and the cholanoate moieties, and a macromonomer (PLA-PEG-PLA-DA), bearing two acryloyl end groups derived from a poly(lactic acid)-b-poly(ethylene glycol)-b-poly(lactic acid) triblock copolymer. The structure of MACAH was confirmed by FTIR, <SUP>1</SUP>H-NMR, and MS. The hydrogel PLA-PEG-PLA-co-MACAH was characterized by scanning electron microscopy and X-ray diffraction. The experiment results showed that the swelling ratios of the hydrogels decreased with the increase of the CA fraction. The investigation on the in vitro degradation of the hydrogel showed that the CA-containing hydrogels degraded much slower than the hydrogels without CA component. The bioactivity of the synthesized hydrogels was assessed by the simulated body fluid method. The observed formation of hydroxyapatite on the scaffold of the hydrogels indicated that the hydrogels possess good bioactivity. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009</P>

Gallic acid attenuates hypertension, cardiac remodeling, and fibrosis in mice with NG-nitro-L-arginine methyl ester-induced hypertension via regulation of histone deacetylase 1 or histone deacetylase 2

Jin, Li,Lin, Ming Quan,Piao, Zhe Hao,Cho, Jae Yeong,Kim, Gwi Ran,Choi, Sin Young,Ryu, Yuhee,Sun, Simei,Kee, Hae Jin,Jeong, Myung Ho Lippincott Williams & Wilkins 2017 Journal of Hypertension Vol.35 No.7

OBJECTIVE:: Gallic acid, a natural chemical found in plants, has been reported to show antioxidant, anticancer, and anti-inflammatory effects. We investigated the efficacy of a short-term or long-term treatment with gallic acid in N-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive mice and the underlying regulatory mechanism. METHODS:: Hypertension was sufficiently induced after 2 weeks of L-NAME administration. Cardiac remodeling was assessed by echocardiography. Hypertrophic markers, transcription factors, and fibrosis-related gene expression were evaluated by quantitative real-time polymerase chain reaction and western blotting. RESULTS:: Gallic acid effectively lowered SBP, regardless of the administration route (intraperitoneal or oral). L-NAME increased the left ventricular (LV) thickness without an increase in the total heart weight. Weekly echocardiography demonstrated that gallic acid significantly reduced LV posterior wall and septum thickness in chronic L-NAME mice from 3 to 7 weeks. The administration of gallic acid to mice showed a dual preventive and therapeutic effect on the L-NAME-induced LV remodeling. The effect was associated with the suppression of the gene expression of hypertrophy markers and the GATA-binding factor 6 (GATA6) transcription factor. Short-term or long-term treatment with gallic acid attenuated cardiac fibrosis and reduced the expression of histone deacetylase 1 and 2 in H9c2 cells and in rat primary cardiac fibroblasts, as well as in vivo. Small interfering RNA knockdown confirmed the association of these enzymes with L-NAME-induced cardiac remodeling and fibrosis. CONCLUSION:: These results suggested that gallic acid may be a potential therapeutic agent for the treatment of cardiovascular diseases with hypertension and cardiac fibrosis.

Nondestructive Assessment of Glycosaminoglycans in Engineered Cartilages Using Hexabrix-Enhanced Micro-Computed Tomography

Jin, Long Hao,Choi, Byung Hyune,Kim, Young Jick,Oh, Hyun Ju,Kim, Byoung Ju,Yin, Xiang Yun,Min, Byoung-Hyun 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.11 No.4

Relationship between Invert-Filling Disengaging and Deformation of Shield Tunnel Using Staggered Assembled Segment

Hao Jin,Jie Su,Chen Zhao 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.4

The disengaging between invert filling and segment affects the safety of train operation. A 3D numerical model of a three-ring staggered assembled shield tunnel is established, which is verified by a full-scale test. On this basis, cohesive element is inserted between the invert filling and tunnel segment to simulate the fracture process of invert filling-segment interface, and explore the relationship between the deformation of staggered assembled shield tunnel and the disengaging of invert filling. The results show that: 1) The development of invert filling disengaging affects the internal forces of shield tunnel segments. 2) The relationship between the development of invert filling disengaging and the deformation of shield tunnel can be four stages. Different segment assembly methods do not affect the variability of the disengaging ratio. Tensile failure occurs in 89% of the interface layer, which is the main form of invert filling disengaging. 3) The relationship between the development of disengaging distance and the deformation of shield tunnel also can be four stages. The faster the horizontal convergent deformation develops in the segment assembly method, the slower the growth rate of the disengaging distance. 4) The faster the horizontal convergent deformation develops in the segment assembly method, the slower the growth rate of the disengaging volume.

Probiotic Properties of Pediococcus acidilactici M76 with Functional Exopolysaccharides and Its Lactic Acid Fermentation of Black Raspberry Extract

Hao Jin,Young-Ran Song,Chan-Mi Lee,Seon-Hye Lee,Sang-Ho Baik 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Pediococcus acidilactici M76 (PA-M76) showed outstanding probiotic properties with high tolerance in acidic GIT environments, broad antimicrobial activity, and high adhesion capability in the intestinal tract of Caenorhabditis elegans. PA-M76 treatment resulted in significant increases of pro-inflammatory cytokine mRNA expression in macrophages. PA-M76 produced 1.62 g/L EPS under optimal lactic acid fermentation conditions. Lactic acid fermentation of BRE by PA-M76 did not significantly affect the total anthocyanin and flavonoid content, except for a significant increase in total polyphenol content compared to non-fermented BRE (NfBRE). However, fBRE exhibited increased DPPH radical scavenging activity, linoleic acid peroxidation inhibition rate, and ABTS scavenging activity of fBRE compared to NfBRE. Among the 28 compounds identified in the GC-MS analysis, esters were present as the major groups. The total concentration of volatile compounds was higher in fBRE than that in NfBRE. However, the undesirable flavor of terpenes decreased. PA-M76 might be useful for preparing functionally enhanced fermented beverages with a higher antioxidant activity of EPS and enhanced flavors.