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Hanseul Oh,Heechul Kim,Meejung Ahn,Chanwoo Jeong,Jinwoo Jeong,Changjong Moon,Taekyun Shin 대한해부학회 2011 Anatomy & Cell Biology Vol.44 No.2
This study evaluated the cellular localization of cyclic AMP-responsive element binding protein-binding protein (CBP) expression in pig retinas during postnatal development. Immunohistochemistry and Western blot analysis were performed on retinal tissue from 2-day-old, 5-week-old, and 6-month-old pigs. Western blot analysis detected the expression of CBP in the retinas of 2-day-old piglets and showed that it was significantly decreased in the retinas of 5-week-old and 6-month-old pigs. Immunohistochemically, CBP was intensely immunostained in protein kinase C alpha (PKCα)-positive-bipolar cells, glutamine synthetase-positive M?ller cells, and in ganglion cells in 2-day-old piglets. CBP was detected weakly in the inner plexiform, outer nuclear, and rod and cone layers. CBP immunoreactivity in the ganglion cell layer was decreased in the retinas of 5-week-old and 6-month-old pigs, while clear CBP expression detected in the neurite of PKCα-positive bipolar cells in the inner nuclear layer. In addition, CBP immunoreactivity in M?ller cells and glial fibrillary acidic protein-positive glial processes was particularly noteworthy in pig retinas, but not in rat retinas. The results indicate that CBP is expressed differentially in the retinal neurons and glial cells according to growth and animal species, and may play an important role in homeostasis in M?ller cells, neurite extention in bipolar cells, and signal transduction in photoreceptor cells in the porcine retina.
Humanized Mice for the Evaluation of Francisella tularensis Vaccine Candidates
( Hanseul Oh ),( C-yoon Kim ),( Chang-hwan Kim ),( Gyeung-haeng Hur ),( Ji Min Lee ),( Seo-na Chang ),( Jae-hak Park ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.1
Francisella tularensis (FT), a highly infectious pathogen, is considered to be a potential biological weapon owing to the current lack of a human vaccine against it. Tul4 and FopA, both outer membrane proteins of FT, play an important role in the bacterium’s immunogenicity. In the present study, we evaluated the immune response of mice―humanized with human CD34+ cells (hu-mice)―to a cocktail of recombinant Tul4 and FopA (rTul4 and rFopA), which were codon-optimized and expressed in Escherichia coli. Not only did the cocktail-immunized hu-mice produce a significant human immunoglobulin response, they also exhibited prolonged survival against an attenuated live vaccine strain as well as human T cells in the spleen. These results suggest that the cocktail of rTul4 and rFopA had successfully induced an immune response in the hu-mice, demonstrating the potential of this mouse model for use in the evaluation of FT vaccine candidates.
( Hanseul Oh ),( C Yoon Kim ),( Chang Hwan Kim ),( Gyeung Haeng Hur ),( Jae Hak Park ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.9
Francisella tularensis is a highly virulent pathogen of humans and other mammals. Moreover, F. tularensis has been designated a category A biothreat agent, and there is growing interest in the development of a protective vaccine. In the present study, we determine the in vitro and in vivo immune responses of a subunit vaccine composed of recombinant peptides Tul4 and FopA from epitopes of the F. tularensis outer membrane proteins. The recombinant peptides with adjuvant CpG induced robust immunophenotypic change of dendritic cell (DC) maturation and secretion of inflammatory cytokines (IL-6, IL-12). In addition, the matured DCs enabled ex vivo proliferation of naive splenocytes in a mixed lymphocyte reaction. Lastly, we determined the in vivo immune response by assessment of antibody production in C57BL/ 6 mice. Total IgG levels were produced after immunization and peaked in 6 weeks, and moreover, Tul4-specific IgG was confirmed in the mice receiving peptides with or without CpG. Based on these results, we concluded that the recombinant peptides Tul4 and FopA have immunogenicity and could be a safe subunit vaccine candidate approach against F. tularensis.
Oh, Hanseul,Ahn, Meejung,Matsumoto, Yoh,Shin, Taekyun The Korean Society of Veterinary Science 2013 大韓獸醫學會誌 Vol.53 No.4
To better understand the role of macrophages in early stages of experimental autoimmune myocarditis (EAM), we compared the expression of inducible nitric oxide synthase (iNOS) and arginase-1, markers for classically activated M1 and alternatively activated M2 macrophages, respectively, in the hearts of EAM-affected and control rats. Immunohistochemical evidence revealed that both iNOS-positive and arginase 1-positive macrophages were found in EAM lesions, while some cells were co-localized with both markers. This finding suggests that the increased level of arginase-1, which is partly from M2 macrophages, contributes to the modulation of EAM, possibly through the reduction of nitric oxide in the lesion.
오가피 분말을 첨가한 요구르트의 품질 특성 및 항산화 활성
오한슬(HanSeul Oh),강성태(SungTae Kang) 한국식품과학회 2015 한국식품과학회지 Vol.47 No.6
본 연구에서는 오가피 분말의 첨가량을 0.5-2% 첨가한 요구르트를 제조한 후 이에 따른 품질을 평가하였다. 오가피 분말 요구르트의 pH를 측정한 결과, 발효 16시간까지는 오가피 분말의 첨가량이 증가할수록 pH가 낮아졌으나, 발효 20시간 후에는 첨가군의 pH가 대조군보다 높았다. 유산균 수 및 점도는 오가피 분말 2% 첨가군이 대조군보다 유의적으로 낮게 나타났다. 요구르트의 색도의 경우 L값은 오가피 분말 첨가량이 증가할수록 감소하였으나 a값과 b값은 오가피 분말의 첨가량이 증가할수록 증가하였다. DPPH radical 소거능에 대한 항산화활성과 가용성 고형분 함량은 오가피 분말의 첨가량이 많을수록 높아지는 경향을 보였다. 오가피 분말 첨가 요구르트의 관능적 품질평가 결과 0.5% 첨가군의 점수가 가장 높게 나타났다. 발효가 완료된 요구르트를 4℃에서 14일간 저장한 결과, 오가피 분말 0.5% 첨가군의 pH, 적정산도 및 유산균 수는 대조군과 유의적인 차이가 나타나지 않았다. 이러한 결과로 보아 요구르트 제조 시 0.5%의 오가피 분말 첨가는 요구르트의 발효와 품질 특성에 부정적인 영향을 미치지 않으며 건강기능성 및 기호도를 향상시키는 것으로 판단된다. This study was performed to examine the quality characteristics and antioxidant activity of the curd yogurt with different contents (0.5-2%, w/w) of acanthopanax powder (AP). The pH decreased with increasing AP content until 16 h, whereas the treated groups showed higher pH levels than the control group after 20 h. The viscosity and the viable cell counts of the yogurt with 2% AP were lower than those of the control group during fermentation, significantly. Color values of AP yogurt were lower in terms of brightness, whereas redness and yellowness values were higher compared to the control group. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and soluble content significantly increased with increasing AP content. Consumer acceptability score of yogurt with 0.5% AP was ranked higher than other yogurts. Yogurt added with 0.5% AP showed no differences in pH, titrable acidity, and viable cell counts compared to the control group after storage at 4℃ for 14 days.
오한슬 ( Hanseul Oh ),김시윤 ( C-yoon Kim ),한주희 ( Ju-hee Han ),김진 ( Jin Kim ),이은혜 ( Eunhye Lee ),전혜련 ( Hailian Quan ),석승혁 ( Seung Hyeok Seok ),정초록 ( Cho-rok Jung ),박재학 ( Jae-hak Park ) 한국동물실험대체법학회 2016 동물실험대체법학회지 Vol.10 No.1
The global cosmetic market in 2014 was estimated to be worth to about 250 billion USD and has continued to grow. In particular, stem-cell based cosmetics, which can be used to repair skin and protect it from aging, have become recently trendy. However, the questionable safety issue of commercial stem-cell cosmetics has become a hot issue. Cosmetics consumers may buy a product without any information about the safety of products whose ingredients may have less than 10 years of commercial history. For the safety verification of cosmetics and ingredients, the classification for eye irritation needs to be conducted essentially. The Bovine Corneal Opacity & Permeability test (BCOP) is recommended as a validated alternative method for eye irritation by the Organization for Economic Co-operation and Development (OECD). Thus, in this study, we evaluated the eye irritation potential of commercial stem-cell based cosmetics by the BCOP assay, Hens Egg Test on the Chorio-Allantoic Membrane (HET-CAM) assay, and Human Umbilical Vein Endothelial cell (HUVEC) test. Our results showed that all 8 test substances were not classified as eye irritants which could cause serious eye damage according to the BCOP assay associated with histopathology and the HET-CAM assay. But four test substances showed to be mild irritants in the HUVEC test. To the best of our knowledge, our results are the first reported data about the classification of the potential eye irritation of stem-cell based cosmetics, and demonstrate that the validated BCOP assay combined with other alternative tests such as the HET-CAM assay and the HUVEC test, may be able to indicate mild to serious eye irritants.