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      • SCISCIESCOPUS

        Effect of H/Ar treatment on ZnO:B transparent conducting oxide for flexible a-Si:H/μc-Si:H photovoltaic modules under damp heat stress

        Jeong, J.S.,Kim, Y.H.,Park, C.K.,Kim, H.D.,Choi, J. Pergamon Press 2016 Microelectronics and reliability Vol.64 No.-

        <P>A flexible amorphous/microcrystalline Si:H (a-Si:H/mu c-Si:H) tandem-junction photovoltaic (PV) module was produced in which a thin film of ZnO:B grown by metalorganic chemical vapor deposition (MOCVD) served as the transparent conducting oxide (TCO). The Hall mobility of ZnO:B is degraded by damp heat, simulated here using the conditions of 85 degrees C at 85% relative humidity; this affects the series resistance and efficiency of the PV module. In this study, ZnO:B was treated by H/Ar plasma to reduce the degradation experienced under damp heat. The degradation time of the Hall mobility of ZnO:B, defined as the time necessary for the cell to reach the efficiency loss of -20%, was improved by similar to 54% by H/Ar treatment (ZnO:B center dot H/Ar). The mechanism behind this improvement was investigated by assessing the reactions of the ZnO:B and ZnO:B center dot H/Ar thin films to moisture. Related changes in the physical and chemical properties of ZnO:B and ZnO:B center dot H/Ar were analyzed by Xray photoelectron spectroscopy, secondary-ion mass spectroscopy, and ultraviolet photoelectron spectroscopy. The analyses showed that the concentration of OH- was high while those of Zn2+ and B3+ were low in the grain boundaries of the ZnO:B surface after exposure to humidity. After H/Ar treatment, the increase in OH- concentration in ZnO:B was reduced, and the decrease in the Zn2+ and B3+ concentrations was much smaller. The H/Ar plasma treatment of ZnO:B affected the surface reaction forming Zn(OH)(2), between the OH- and Zn2+ ions at the grain boundaries under damp heat. (C) 2016 Elsevier Ltd. All rights reserved.</P>

      • SCISCIESCOPUS

        Surveillance of avian influenza virus in wild bird fecal samples from South Korea, 2003-2008.

        Kang, H M,Jeong, O M,Kim, M C,Kwon, J S,Paek, M R,Choi, J G,Lee, E K,Kim, Y J,Kwon, J H,Lee, Y J [Wildlife Disease Association] 2010 JOURNAL OF WILDLIFE DISEASES Vol.46 No.3

        <P>We analyzed the results from nationwide surveillance of avian influenza (AI) from birds in South Korea's major wild bird habitats and the demilitarized zone of South Korea, 2003-2008. Of 28,214 fecal samples analyzed, 225 yielded influenza viruses, for a prevalence of 0.8%. Hemagglutinin (HA) subtypes H1-H12 and all nine neuraminidase (NA) subtypes were detected. The dominant HA subtypes were H6, H1, and H4, and the most common NA subtypes were N2, N1, and N6. Among the 38 HA/NA subtype combinations, the most common were H4N6, H6N1, and H5N2. Thirty-seven low-pathogenic AI (LPAI) viruses of the H5 and H7 subtype were detected. Among them, we identified bird species for 16 H5- and H7-positive fecal samples using a DNA bar-coding system instituted in 2007; all birds were identified as Anseriformes. The HA gene of the H5 wild bird isolates belonged to the Eurasian avian lineage, and could be clearly distinguished from the sublineage H5N1 highly pathogenic AI (HPAI) of the Eurasian and American avian lineages. Whereas H7 LPAI viruses did not group as a separate sublineage with H7 HPAI viruses, H7 isolates were closely related with the Eurasian avian lineage.</P>

      • SCIESCOPUS

        Genetic and phylogenetic characterizations of a novel genotype of highly pathogenic avian influenza (HPAI) H5N8 viruses in 2016/2017 in South Korea

        Kim, Y.I.,Park, S.J.,Kwon, H.I.,Kim, E.H.,Si, Y.J.,Jeong, J.H.,Lee, I.W.,Nguyen, H.D.,Kwon, J.J.,Choi, W.S.,Song, M.S.,Kim, C.J.,Choi, Y.K. Elsevier Science 2017 INFECTION GENETICS AND EVOLUTION Vol.53 No.-

        <P>During the outbreaks of highly pathogenic avian influenza (HPAI) H5N6 viruses in 2016 in South Korea, novel H5N8 viruses were also isolated from migratory birds. Phylogenetic analysis revealed that the HA gene of these H5N8 viruses belonged to clade 2.3.4.4, similarly to recent H5Nx viruses, and originated from A/Brk/Korea/Gochang1/14(H5N8), a minor lineage of H5N8 that appeared in 2014 and then disappeared. At least four reassortment events occurred with different subtypes (H5N8, H7N7, H3N8 and H10N7) and a chicken challenge study revealed that they were classified as HPAI viruses according to OIE criteria. (C) 2017 Elsevier B.V. All rights reserved.</P>

      • 광음향효과를 이용한 MnSO₄ㆍ4H₂O,MnC₂ㆍ4H₂O,CuSO₄ㆍ5H₂O,Al₂O₃ : Cr의 전자상자성 공명 검출 Cr Crystals

        윤수인,김학수,최규만,정중현 부산대학교 물성연구소 1985 물성연구소연구논문집 Vol.4 No.-

        광음향효과를 이용하여 MnSO₄ㆍ4H₂O, MnCl₂ㆍ4H₂O, CuSO₄ㆍ5H₂O 및 Al₂O₃ : Cr 결정의 상자성 공명을 검출했다. X-밴드 마이크로파의 도파관 내에 광음향 cell을 장치하고 관내에 넣은 short의 위치를 조절하여 도파관 자체가 공명공동이 되도록 함으로써 장치의 감도를 개선했다. g값과 공명신호의 자기장반가폭으로 MnSO₄ㆍ4H₂O의 Mn²^(+)로 부터 1.99±0.03과 260 gauss를, CuSO₄ 5H₂O의 Cu²^(+)로부터 2.31±0.02와 200 gauss를, MnCl₂ 4H₂O로부터 2.06±0.04와 690 gauss를 그리고 Al₂O₃:Cr의 Cr³^(+)로부터 1.97±0.03과 480 gauss를 얻었다. The electron paramagnetic resonances were observed at room temperature in concentrated system, MnSO₄ㆍ4H₂O, MnCl₂ㆍ4H₂O and CuSO₄ㆍ5H₂O and in the dilute system, Al₂O₃: Cr, using photoacoustic technique. A commercial x-band microwave system was modified by introducing the photoacoustic (PA) cell into the microwave cavity to improve the sensitivity of the PA system. The observed g-values and half-widths were 1.99±0.03 and 260 gauss for Mn²^(+) in MnSO₄ㆍ4H₂O crystal, 2.31±0.02 and 200 gauss for Cu²^(+) in CuSO₄ 5H₂O crystal, 2.06±0.04 and 690 gauss for MnCl2 4H₂O crystal, and 1.97±0.03 and 480 gauss for CrCr³^(+) in Al₂O₃:Cr crystal, respectively.

      • SCISCIESCOPUS

        Validation of egg yolk antibody based C-ELISA for avian influenza surveillance in breeder duck

        Jeong, O.M.,Kim, M.C.,Kang, H.M.,Ha, G.W.,Oh, J.S.,Yoo, J.E.,Park, C.H.,Kwon, J.S.,Pack, M.R.,Kim, H.R.,Kim, Y.J.,Kwon, J.H.,Lee, Y.J. Elsevier Scientific Pub. Co 2010 Veterinary microbiology Vol.144 No.3

        Active surveillance for avian influenza virus (AIV) has expanded from chicken to various poultry species including duck. To further effective antibody screening in laying breeder ducks, we validated the egg yolk antibody as alternative source to serum for AIV antibody. Sera and eggs were collected at weekly intervals after two types of AIV vaccination, H5N3 and H9N2. The antibody levels were determined by an agar gel immunodiffusion (AGID) test, haemagglutination inhibition (HI) test and the competitive enzyme-linked immunosorbent assay (C-ELISA). AGID test did not detect antibodies in egg yolk, and the agreement between AGID test and either HI test or C-ELISA in serum was slight and fair based on kappa statistics (kappa value (κ)@?0.19 in H5N3 group and κ@?0.37 in H9N2 groups). However, there was almost perfect agreement between HI test and C-ELISA (κ>0.9 in all group). The C-ELISA was as sensitive and specific as the HI test, and could be used as a pre-screening test for the detection of type A avian influenza virus antibody. Comparison was made between egg yolk and serum antibody titers by a regression analysis. A high correlation was observed between serum and yolk antibody titers (r=0.8762 for H5N3 and 0.8914 for H9N2 in HI test; r=1 for H5N3 and 0.9686 for H9N2 in ELISA test), although egg yolk antibodies were detected later and remained lower levels than serum antibodies. In field trials involving 54 duck flocks, the positive rate of egg yolk and serum samples showed agreement for the detection of AIV antibody. We concluded that as an alternative to serum, antibody monitoring of laying breeder duck using egg yolk with C-ELISA is feasible and is recommended.

      • Ex situ catalytic upgrading of lignocellulosic biomass components over vanadium contained H-MCM-41 catalysts

        Kim, B.S.,Jeong, C.S.,Kim, J.M.,Park, S.B.,Park, S.H.,Jeon, J.K.,Jung, S.C.,Kim, S.C.,Park, Y.K. Elsevier Science Publishers 2016 CATALYSIS TODAY - Vol.265 No.-

        <P>H-V-MCM-41 catalysts containing 5, 10, and 30 wt% of vanadium were synthesized and applied to the ex situ catalytic pyrolysis (CP) of three polymeric components of lignocellulosic biomass for the first time. Characterization of the catalysts was performed using N-2 adsorption-desorption, XRD, FT-IR, and NH3-TPD. The results of XRD analysis showed that 5 wt% and 10 wt% H-V-MCM-41 catalysts maintained the mesoporous structure, whereas the mesoporous structure was destroyed in 30 wt% H-V-MCM-41 with considerable amount of small V2O5 crystalline outside the framework. NH3-TPD showed that H-V-MCM-41 has mostly weak acid sites and that 10 wt% H-V-MCM-41 had the largest quantity of acid sites due to framework vanadium. In the case of CP of cellulose using Py-GC/MS, 10 wt% H-V-MCM-41 showed the highest catalytic activity for the production of valuable furanic compounds such as furfural because of the enhanced deoxygenation over the acid sites formed on framework vanadium. In the case of CP of xylan as well, 10 wt% H-V-MCM-41 led to the largest yield of mono-aromatics. The production of acetic acid was also promoted by H-V-MCM-41 catalysts. The CP of lignin over H-V-MCM-41 catalysts promoted substantially the production of important feedstock chemicals for the petrochemical industry: phenolics and mono-aromatics. (C) 2015 Elsevier B.V. All rights reserved.</P>

      • KCI등재

        Simultaneous subtyping and pathotyping of the novel reassortant influenza A (H5N8) virus from clinical samples using a diagnostic microarray

        Kwon, J. H.,Kim, J. H.,Lee, D. h.,Cho, H.,Hwang, S. Y.,Yuk, S. S.,Erdene-Ochir, T. O.,Noh, J. Y.,Hong, W. T.,Jeong, J. H. Springer Science + Business Media 2016 BioChip Journal Vol.10 No.3

        <P>Highly pathogenic avian influenza (HPAI) viruses cause economic losses in the poultry industry and pose a severe threat to human health. Rapid and accurate diagnostic methods are important because they can help prevent further spread of the virus and reduce the time required for eradication of the virus. We developed a low-density microarray for the rapid detection and identification of avian influenza virus subtypes H5, H7, and H9 and their pathotypes in a previous study. In the present study, we report the development of updated probe sets and evaluation of the diagnostic microarray using H5N8 clade 2.3.4.4 HPAI viruses including clinical samples, without the need for egg propagation. Cy3-labeled DNA targets were obtained by reverse transcription polymerase chain reaction using Cy3-labeled universal primers, and labeled amplicons were hybridized to the microarray. All positive samples from RT-PCR showed H5-specific and highly pathogenic pattern in the microarray, without purification of PCR products. Furthermore, it allowed for specific detection of the subtype and pathotype from low DNA concentration samples that did not allow direct sequence analysis. Therefore, this diagnostic microarray has enormous potential for the rapid subtyping and pathotyping from clinical samples without the need for culture.</P>

      • SCISCIESCOPUS

        Effect of pre-annealing on the phase formation and efficiency of CZTS solar cell prepared by sulfurization of Zn/(Cu,Sn) precursor with H<sub>2</sub>S gas

        Lee, J.H.,Choi, H.J.,Kim, W.M.,Jeong, J.H.,Park, J.K. Association for Applied Solar Energy ; Elsevier Sc 2016 SOLAR ENERGY -PHOENIX ARIZONA THEN NEW YORK- Vol.136 No.-

        The effect of pre-annealing on the phase formation behavior and efficiency of CZTS thin film solar cell prepared by sulfurization of sputtered Zn/(Cu,Sn) metal precursor with H<SUB>2</SUB>S gas was investigated. Precursor with stacking structure of Zn/(Cu,Sn) was deposited by sputtering of Cu, Zn, and Sn metal targets. The depth profile of metal elements and cell efficiency of the sulfurized CZTS films with H<SUB>2</SUB>S were observed to be critically dependent on the pre-annealing conditions. For the CZTS film prepared by sulfurization in N<SUB>2</SUB>-5vol.% H<SUB>2</SUB>S at 550<SUP>o</SUP>C after pre-annealing at 350<SUP>o</SUP>C in Ar, segregation of SnS phase at the surface region was observed to be pronounced. When the pre-annealing was performed at 350<SUP>o</SUP>C in N<SUB>2</SUB>-5vol.% H<SUB>2</SUB>S, however, uniform depth profile of metal elements with a small amount of CuS phase was observed. The CuS phase was disappeared with increase in the pre-annealing temperature in N<SUB>2</SUB>-5vol.% H<SUB>2</SUB>S. The phase formation behavior influenced by pre-annealing condition was observed to affect solar cell performance of the CZTS thin film synthesized at 550<SUP>o</SUP>C in N<SUB>2</SUB>-5vol.% H<SUB>2</SUB>S. In contrast to the CZTS thin film prepared with pre-annealing at 350<SUP>o</SUP>C in Ar showing bad efficiency (~0.93%), the CZTS solar cells fabricated with pre-annealing at 450<SUP>o</SUP>C in H<SUB>2</SUB>S shows higher efficiency of 3.04%. By the optimization of Zn layer thickness, solar cell efficiency of 4.40% was obtained in the CZTS thin film prepared with pre-annealing at 450<SUP>o</SUP>C in N<SUB>2</SUB>-5vol.% H<SUB>2</SUB>S. This phenomenon was due to the change in the secondary phase formation behavior during sulfurization of the Zn/(Cu,Sn) metal precursor with various pre-annealing conditions.

      • Display of membrane proteins on the heterologous caveolae carved by caveolin-1 in the Escherichia coli cytoplasm

        Shin, J.,Jung, Y.H.,Cho, D.H.,Park, M.,Lee, K.E.,Yang, Y.,Jeong, C.,Sung, B.H.,Sohn, J.H.,Park, J.B.,Kweon, D.H. IPC Science and Technology Press ; Elsevier Scienc 2015 Enzyme and microbial technology Vol.79 No.-

        Caveolae are membrane-budding structures that exist in many vertebrate cells. One of the important functions of caveolae is to form membrane curvature and endocytic vesicles. Recently, it was shown that caveolae-like structures were formed in Escherichia coli through the expression of caveolin-1. This interesting structure seems to be versatile for a variety of biotechnological applications. Targeting of heterologous proteins in the caveolae-like structure should be the first question to be addressed for this purpose. Here we show that membrane proteins co-expressed with caveolin-1 are embedded into the heterologous caveolae (h-caveolae), the cavaolae-like structures formed inside the cell. Two transmembrane SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins, Syntaxin 1a and vesicle-associated membrane protein 2 (VAMP2), were displayed on the h-caveolae surface. The size of the h-caveolae harboring the transmembrane proteins was ~100nm in diameter. The proteins were functional and faced outward on the h-caveolae. Multi-spanning transmembrane proteins FtsH and FeoB could be included in the h-caveolae, too. Furthermore, the recombinant E. coli cells were shown to endocytose substrate supplemented in the medium. These results provide a basis for exploiting the h-caveolae formed inside E. coli cells for future biotechnological applications.

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