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Analysis of Phenolic compound and isoflavone in various medicinal soybeans
Jae-woo Kim,Eun-young Kang,Su-hyun Seo,Eun-hye Kim,Tae-sic Gwak,Hyun-seok Jeon,Joung-kuk Ahn,Woo-seok Jung,Ill-min Chung 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
Medicinal soybean, which has been proven to be effective in preventing cancer, heart disease, osteoporosis, and renal failure, is drawing people’s attention among other beans in recent days. This experiment was to examine the concentration of phenolic compounds and isoflavone in 53 kinds of soybeans cultivated and collected in Yeo-Ju, Gyeonggi-do region. The total average content of phenol is 29424.34㎍/g and the most and least phenol were detected in No. 35(814.98㎍/g) and No. 28(353.66㎍/g) respectively. Myricetin was detected the most resveratrol whereas detected the least amount which was less than 0.2% of the total amount among all medicinal soybean samples. Total average isoflavone content was 35910.56㎍/g and the most and least isoflavone was detected in No. 15 (996.66 ㎍/g ) and No.9 (476.18㎍/g )respectively . Malonylgenistin was detecteded the most genistein whereas detected the least amount which was less than 0.02 % of the total amount in all medicinal soybean samples. The result of this experiment is considered to be the basic data of the functional food manufacturing by utilizing domestic medicinal soybean.
Evaluation of isoflavones and phenolic compounds in Korea soybean seeds
Su-hyun Seo,Eun-hye Kim,Eun-young Kang,Jae-woo Kim,Tae-sic Gwak,Hyun-seok Jeon,Joung-kuk Ahn,Woo-seok Jung,Ill-min Chung 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
Soybean(Glycine max(L.) Merill) is a good source of vegetable oil and high-quality protein. Soy foods have became popular food worldly because of its ingredients. Recently, as the well-being trends, the demand for soybean has increased in the West as well as in Asian countries. It contains valuable source of dietary isoflavones and phenolic compounds which are important secondary metabolites. The 33 germplasm cultivated in Yeoju, Gyeonggi-do region. The obtained results showed that isoflavones and phenolic compounds were significantly various. The highest compounds is malonyl genistin (230.89㎍-1), the lowest is genistin(0.70㎍-1) and the highest germplasm is Dunggeunseolitaekong (2222.60㎍-1) in isoflavone. In phenolic compounds, the highest compounds is myricetin (155.59㎍-1) and the lowest is vanillin (1.89㎍-1). Total containment of phenolic compounds is the highest in Dunggeunseolitaekong (803.76㎍-1) germplasm.
P284 : Association between an HLA-DQB1 polymorphism and non-segmental vitiligo
( Eun Jae Shin ),( Min Jae Gwak ),( Sung Hyuk Moon ),( Ki Heon Jeong ),( Su Kang Kim ),( Joo Ho Chung ),( Min Kyung Shin ),( Mu Hyoung Lee ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: Vitiligo is pigmentary skin disorders characterised by depigmented maculopatches because of melanocyte destruction. Although the etiology of vitiligo is currently unknown, several theories have been proposed to explain the pathogenesis of this disease, including autoimmune, neural, self-destruction, oxidative stress, and genetic theories. Recent genome wide association studies highlighted significant relations with human leukocyte antigen (HLA) region and generalized vitiligo. Objectives: The purpose of this study was to determine whether variation in the HLA-DQB1 gene contributes to the risk of developing non-segmental vitiligo (NSV) in the Korean population. Methods: We conducted a case-control association study of 130 NSV patients and 445 matched, unaffected controls. A total of four single nucleotide polymorphisms (SNPs) in HLA-DQB1 gene were selected for study. Results: Of the selected SNPs, one SNP (rs9274552) showed significant genotypic differences between the NSV patient group and the control group. In haplotype analysis, CG and AG, consisting of rs9274552, and rs9274579, demonstrated a significant association with NSV. Conclusion: These results suggest that promoter polymorphisms of HLA-DQB1 are associated with the development of NSV in the Korean population.
삼나무 건조 중 발생하는 휘발성 유기화합물 Terpenoids의 분석
이수연 ( Su Yeon Lee ),곽기섭 ( Ki Seob Gwak ),김선홍 ( Seon Hong Kim ),이전제 ( Jun Jae Lee ),여환명 ( Hwan Myeong Yeo ),최인규 ( In Gyu Choi ) 한국목재공학회 2010 목재공학 Vol.38 No.3
본 연구는 삼나무의 건조 중 발생하는 Volatile Organic Compounds (VOCs) 중 terpenoids 성분을 분석하기 위해 thermal extractor를 이용하였다. 기존의 건조 조건을 고려하여 27°C, 60°C, 80°C, 100°C, 120°C에서 실험을 실시한 결과 온도 조건이 높아질수록 VOCs와 terpenoids의 방산량이 증가하였다. 방산된 VOCs 중 terpenoids 화합물이 차지하는 비율은 각각의 온도에 대해 87.5%, 81.6%, 83.6%, 90.1%, 97.3%로 특히 100°C와 120°C에서는 90% 이상의 높은 비율을 차지하였다. 상대적으로 가장 높은 비율을 차지하고 있는 성분은 δ-cadinene이었으며 이 외에도 총 32종류의 다양한 terpenoids 성분이 검출되었다. 특히 방산된 성분들은 모두 탄소 15개로 이루어진 sesquiterpene 류에 속하는 성분으로서 삼나무를 이루는 주요 성분임을 확인하였다. 방산된 sesquiterpenes 화합물의 이용을 모색하고자 피부사상균에 대한 항진균 활성을 한천희석법을 이용하여 평가해 보았다. 그 결과 공시 균주 Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum gypseum에 대해 모두 활성을 나타냈다. 시료의 농도가 5000 ppm일 때에는 3 균주에 대해 모두 100%의 활성을 나타냈으며 1000 ppm일 때에는 95.2%, 98.7%, 97.3%의 활성을, 100 ppm일 때에는 90.5%, 43.6%, 85.9%의 활성을 각각 보였다. The aim of this study was to investigate the terpenoids of Total Volatile Organic Compounds (VOCs) released during drying of Cryptomeria japonica using the thermal extractor (TE). Considering the drying process of C. japonica, temperatures of TE were set at 27°C, 60°C, 80°C, 100°C, and 120°C, respectively. As the result, the emission factors of VOCs and terpenoids were increased as temperature increased. The amount of terpenoids included in VOCs emission factors were 87.5%, 81.6%, 83.6%, 90.1%, and 97.3% depending on above temperatures, respectively. Especially at 100°C and 120°C, the amount of terpenoids were measured more than 90%. δ-cadinene was the highest yield at each temperature and 32 types of terpenoids were collected. Emitted terpenoids were classified into the sesquiterpene group which consists of 15 carbon sources. These 32 sesquiterpenes were used for determining the useful bioactivity such as antifungal activity by the agar dilution. As the result, they showed the antifungal activity against Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum gypseum. The 5,000 ppm concentration of terpenoids showed a strong activity with 100% against the 3 fungi. At the 1,000 ppm concentration of terpenoids, the antifungal activities against three fungi were 95.2%, 98.7%, and 97.3%, and their activities were a little inhibited at 100 ppm concentration.