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- 검색키워드
- 저자 : Geon-Yeop Do (검색결과 16 건)
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Geon-Yeop Do,Jin-Woo Kim,Sung-Kyu Chae,Jae-Hyun Ahn,박효진,Jae-Young Park,Seul-Gi Yang,구덕본 사단법인 한국동물생명공학회 2015 한국동물생명공학회지 Vol.30 No.4
Edaravone (Eda) is a potent scavenger of inhibiting free radicals including hydroxyl radicals (H2O2). Reactive oxygen species (ROS) such as H2O2 can alter most kinds of cellular molecules such as lipids, proteins and nucleic acids, cellular apoptosis. In addition, oxidative stress from over-production of ROS is involved in the defective embryo development of porcine. Previous study reported that Eda has protective effects against oxidative stress-like cellular damage. However, the effect of Eda on the preimplantation porcine embryos development under oxidative stress is unclear. Therefore, in this study, the effects of Eda on blastocyst development, expression levels of ROS, and apoptotic index were first investigated in preimplantation porcine embryos. After in vitro fertilization, porcine embryos were cultured for 6 days in PZM medium with Eda (10 μM), H2O2 (200 μM), and Eda+H2O2 treated group, respectively. Rate of blastocyst development was significantly increased (P<0.05) in the Eda treated group compared with only H2O2 treated group. And, we measured intracellular levels of ROS by DCF-DA staining methods and investigated numbers of apoptotic nuclei by TUNEL assay analysis is in porcine blastocyst, respectively. Both intracellular ROS levels and the numbers of apoptotic nucleic were significantly decreased (P<0.05) in porcine blastocysts cultured with Eda (10 μM). More over, the total cell number of blastocysts were significantly increased (P<0.05) in the Eda-treated group compared with untreated group and the only H2O2 treated group. Based on the results, Eda was related to regulate as antioxidant-like function according to the reducing ROS levels during preimplantation periods. Also, Eda is beneficial for developmental competence and preimplantation quality of porcine embryos. Therefore, we concluded that Eda has protective effect to ROS derived apoptotic stress in preimplantation porcine embryos.
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Do, Geon-Yeop,Kim, Jin-Woo,Park, Hyo-Jin,Yoon, Seung-Bin,Park, Jae-Young,Yang, Seul-Gi,Jung, Bae Dong,Kwon, Yong-Soo,Kang, Man-Jong,Song, Bong-Seok,Kim, Sun-Uk,Chang, Kyu-Tae,Koo, Deog-Bon Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.9
Objective: Phellodendron amurense (P. amurense) and Humulus japonicus (H. japonicus) are closely involved in anti-oxidative response and increasing antioxidant enzymes activities. However, the effects of their extracts on development of preimplantation bovine embryos have not been investigated. Therefore, we investigated the effects of P. amurense and H. japonicus extracts on developmental competence and quality of preimplantation bovine embryos. Methods: After in vitro fertilization, bovine embryos were cultured for 7 days in Charles Rosenkrans amino acid medium supplemented with P. amurense ($0.01{\mu}g/mL$) and H. japonicus ($0.01{\mu}g/mL$). The effect of this supplementation during in vitro culture on development competence and antioxidant was investigated. Results: We observed that the blastocysts rate was significantly increased (p<0.05) in P. amurense ($28.9%{\pm}2.9%$), H. japonicus ($30.9%{\pm}1.5%$), and a mixture of P. amurense and H. japonicus ($34.8%{\pm}2.1%$) treated groups compared with the control group ($25.4%{\pm}1.6%$). We next confirmed that the intracellular levels of reactive oxygen species (ROS) were significantly decreased (p<0.01) in P. amurense and/or H. japonicus extract treated groups when compared with the control group. Our results also showed that expression of cleaved caspase-3 and apoptotic cells of blastocysts were significantly decreased (p<0.05) in bovine blastocysts derived from both P. amurense and H. japonicus extract treated embryos. Conclusion: These results suggest that proper treatment with P. amurense and H. japonicus extracts in the development of preimplantation bovine embryos improves the quality of blastocysts, which may be related to the reduction of ROS level and apoptosis.
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Do, Geon-Yeop,Kim, Jin-Woo,Chae, Sung-Kyu,Ahn, Jae-Hyun,Park, Hyo-Jin,Park, Jae-Young,Yang, Seul-Gi,Koo, Deog-Bon The Korean Society of Embryo Transfer 2015 한국동물생명공학회지 Vol.30 No.4
Edaravone (Eda) is a potent scavenger of inhibiting free radicals including hydroxyl radicals ($H_2O_2$). Reactive oxygen species (ROS) such as $H_2O_2$ can alter most kinds of cellular molecules such as lipids, proteins and nucleic acids, cellular apoptosis. In addition, oxidative stress from over-production of ROS is involved in the defective embryo development of porcine. Previous study reported that Eda has protective effects against oxidative stress-like cellular damage. However, the effect of Eda on the preimplantation porcine embryos development under oxidative stress is unclear. Therefore, in this study, the effects of Eda on blastocyst development, expression levels of ROS, and apoptotic index were first investigated in preimplantation porcine embryos. After in vitro fertilization, porcine embryos were cultured for 6 days in PZM medium with Eda ($10{\mu}M$), $H_2O_2$ ($200{\mu}M$), and Eda+$H_2O_2$ treated group, respectively. Rate of blastocyst development was significantly increased (P<0.05) in the Eda treated group compared with only $H_2O_2$ treated group. And, we measured intracellular levels of ROS by DCF-DA staining methods and investigated numbers of apoptotic nuclei by TUNEL assay analysis is in porcine blastocyst, respectively. Both intracellular ROS levels and the numbers of apoptotic nucleic were significantly decreased (P<0.05) in porcine blastocysts cultured with Eda ($10{\mu}M$). More over, the total cell number of blastocysts were significantly increased (P<0.05) in the Eda-treated group compared with untreated group and the only $H_2O_2$ treated group. Based on the results, Eda was related to regulate as antioxidant-like function according to the reducing ROS levels during preimplantation periods. Also, Eda is beneficial for developmental competence and preimplantation quality of porcine embryos. Therefore, we concluded that Eda has protective effect to ROS derived apoptotic stress in preimplantation porcine embryos.
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Geon-Yeop Do,Jin-Woo Kim,Sung-Kyu Chae,Jae-Hyun Ahn,Hyo-Jin Park,Jae-Young Park,Seul-Gi Yang,Deog-Bon Koo 한국수정란이식학회 2015 한국동물생명공학회지 Vol.30 No.4
Edaravone (Eda) is a potent scavenger of inhibiting free radicals including hydroxyl radicals (H2O2). Reactive oxygen species (ROS) such as H2O2 can alter most kinds of cellular molecules such as lipids, proteins and nucleic acids, cellular apoptosis. In addition, oxidative stress from over-production of ROS is involved in the defective embryo development of porcine. Previous study reported that Eda has protective effects against oxidative stress-like cellular damage. However, the effect of Eda on the preimplantation porcine embryos development under oxidative stress is unclear. Therefore, in this study, the effects of Eda on blastocyst development, expression levels of ROS, and apoptotic index were first investigated in preimplantation porcine embryos. After in vitro fertilization, porcine embryos were cultured for 6 days in PZM medium with Eda (10 μM), H2O2 (200 μM), and Eda+H2O2 treated group, respectively. Rate of blastocyst development was significantly increased (P<0.05) in the Eda treated group compared with only H2O2 treated group. And, we measured intracellular levels of ROS by DCF-DA staining methods and investigated numbers of apoptotic nuclei by TUNEL assay analysis is in porcine blastocyst, respectively. Both intracellular ROS levels and the numbers of apoptotic nucleic were significantly decreased (P<0.05) in porcine blastocysts cultured with Eda (10 μM). More over, the total cell number of blastocysts were significantly increased (P<0.05) in the Eda-treated group compared with untreated group and the only H2O2 treated group. Based on the results, Eda was related to regulate as antioxidant-like function according to the reducing ROS levels during preimplantation periods. Also, Eda is beneficial for developmental competence and preimplantation quality of porcine embryos. Therefore, we concluded that Eda has protective effect to ROS derived apoptotic stress in preimplantation porcine embryos.
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Anti-Apoptotic Effects of Catalpol on Preimplantaion Porcine Embryos
Lee, Yong-Hee,Kim, Jin-Woo,Chae, Sung-Kyu,Ahn, Jae-Hyun,Do, Geon-Yeop,Koo, Deog-Bon The Korean Society of Embryo Transfer 2015 한국동물생명공학회지 Vol.30 No.1
Catalpol, an iridoid glucoside, isolated from the root of Rehmannia glutinosa Libosch. It possesses a broad range of biological and pharmacological activity including anti-tumor, anti-inflammation and anti-oxidant by acting as a free radical scavenger. Therefore, in this study, the effects of catalpol on blastocyst development, expression levels of reactive oxygen species (ROS) and apoptotic index were investigated in porcine embryos. After in vitro maturation and fertilization, porcine embryos were cultured for 6 days in porcine zygote medium 3 (PZM-3) supplemented with catalpol (0, 100, 200 and $400{\mu}M$, respectively). Blastocyst development not significantly improved in the catalpol treated group when compared with control group. Otherwise, the intracelluar levels of ROS were decreased and the numbers of apoptotic nuclei were reduced in the catalpol ($100{\mu}M$) treated porcine blastocysts (P<0.05). On the other hand, blastocyst development was significantly improved in the catalpol ($100{\mu}M$) treated group when compared with the untreated catalpol group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress (P<0.05). Otherwise, the intracellular levels of ROS in catalpol ($100{\mu}M$) treated group were significantly decreased in the untreated catalpol group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress (P<0.05). Furthermore, the total cell numbers of blastocysts were significantly increased (P<0.05) in the catalpol ($100{\mu}M$) treated group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress, whereas numbers of apoptoic nuclei were significantly reduced (P<0.05). In conclusion, our results indicate that treatment of catalpol may have important implications for improving developmental competence and preimplantation quality of porcine embryos through its anti-oxidant and anti-apoptotic effect.
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KIM, Jin-Woo,PARK, Hyo-Jin,CHAE, Sung-Kyu,AHN, Jae-Hyun,DO, Geon-Yeop,CHOO, Young-Kug,PARK, Joung Jun,JUNG, Bae Dong,KIM, Sun-Uk,CHANG, Kyu-Tae,KOO, Deog-Bon 家畜繁殖硏究所 2016 Journal of Reproduction and Development Vol.62 No.3
<P> Gangliosides are key lipid molecules required for the regulation of cellular processes such as proliferation, differentiation, and cell signaling, including signaling of epidermal growth factor receptor (EGFR). Epidermal growth factor (EGF) has long been considered a potential regulator of meiotic and cytoplasmic maturation in mammalian oocytes. However, there is no report on the direct effect of ganglioside GD1a in porcine oocyte maturation. In this study, we first investigated a functional link between GD1a and meiotic maturation during <I>in vitro</I> maturation (IVM) of porcine embryos. Moreover, we confirmed the effect of exogenous GD1a treatment on blastocyst development, quality, and fertilization rate in early embryonic development. First, we observed that the protein level of ST3GAL2, a GD1a synthesizing enzyme, significantly increased (P < 0.01) in cumulus-oocyte-complexes (COCs) during IVM progress. The proportion of arrested germinal vesicles (GV) increased in oocytes treated with EGF+GD1a (41.6 ± 1.5%) at the IVM I stage. Upon completion of meiotic maturation, the proportion of metaphase II (M II) was significantly higher (P < 0.05) in the EGF+GD1a (89.9 ± 3.6%) treated group. After IVF, the percentage of penetrated oocytes was significantly higher (P < 0.05) in the EGF+GD1a (89.1 ± 2.3%) treated group than in the control group. Furthermore, exogenous GD1a treatment improved the developmental competence and quality of blastocysts during preimplantation embryo development stage. These results suggest that ganglioside GD1a may play an important role in IVM mechanisms of porcine maturation capacity. Furthermore, our findings will be helpful for better promoting the embryo development and blastocyst quality in pigs.</P>
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Anti-Apoptotic Effects of Catalpol on Preimplantaion Porcine Embryos
Yong-Hee Lee,Jin-Woo Kim,Sung-Kyu Chae,Jae-Hyun Ahn,Geon-Yeop Do,Deog-Bon Koo 한국수정란이식학회 2015 한국동물생명공학회지 Vol.30 No.1
Catalpol, an iridoid glucoside, isolated from the root of Rehmannia glutinosa Libosch. It possesses a broad range of biological and pharmacological activity including anti-tumor, anti-inflammation and anti-oxidant by acting as a free radical scavenger. Therefore, in this study, the effects of catalpol on blastocyst development, expression levels of reactive oxygen species (ROS) and apoptotic index were investigated in porcine embryos. After in vitro maturation and fertilization, porcine embryos were cultured for 6 days in porcine zygote medium 3 (PZM-3) supplemented with catalpol (0, 100, 200 and 400 μM, respectively). Blastocyst development not significantly improved in the catalpol treated group when compared with control group. Otherwise, the intracelluar levels of ROS were decreased and the numbers of apoptotic nuclei were reduced in the catalpol (100 μM) treated porcine blastocysts (P<0.05). On the other hand, blastocyst development was significantly improved in the catalpol (100 μM) treated group when compared with the untreated catalpol group under H2O2 (200 μM) induced oxidative stress (P<0.05). Otherwise, the intracellular levels of ROS in catalpol (100 μM) treated group were significantly decreased in the untreated catalpol group under H2O2 (200 μM) induced oxidative stress (P<0.05). Furthermore, the total cell numbers of blastocysts were significantly increased (P<0.05) in the catalpol (100 μM) treated group under H2O2 (200 μM) induced oxidative stress, whereas numbers of apoptoic nuclei were significantly reduced (P<0.05). In conclusion, our results indicate that treatment of catalpol may have important implications for improving developmental competence and preimplantation quality of porcine embryos through its anti-oxidant and anti-apoptotic effect
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Anti-Apoptotic Effects of Catalpol on Preimplantaion Porcine Embryos
Yong-Hee Lee,Jin-Woo Kim,Sung-Kyu Chae,Jae-Hyun Ahn,,Geon-Yeop Do,Deog-Bon Koo 사단법인 한국동물생명공학회 2015 한국동물생명공학회지 Vol.30 No.1
Catalpol, an iridoid glucoside, isolated from the root of Rehmannia glutinosa Libosch. It possesses a broad rangeof biological and pharmacological activity including anti-tumor, anti-inflammation and anti-oxidant by acting as a freeradical scavenger. Therefore, in this study, the effects of catalpol on blastocyst development, expression levels ofreactive oxygen species (ROS) and apoptotic index were investigated in porcine embryos. After in vitro maturationand fertilization, porcine embryos were cultured for 6 days in porcine zygote medium 3 (PZM-3) supplemented withcatalpol (0, 100, 200 and 400 μM, respectively). Blastocyst development not significantly improved in the catalpoltreated group when compared with control group. Otherwise, the intracelluar levels of ROS were decreased and thenumbers of apoptotic nuclei were reduced in the catalpol (100 μM) treated porcine blastocysts (P<0.05). On the otherhand, blastocyst development was significantly improved in the catalpol (100 μM) treated group when compared withthe untreated catalpol group under H2O2 (200 μM) induced oxidative stress (P<0.05). Otherwise, the intracellular levelsof ROS in catalpol (100 μM) treated group were significantly decreased in the untreated catalpol group under H2O2(200 μM) induced oxidative stress (P<0.05). Furthermore, the total cell numbers of blastocysts were significantlyincreased (P<0.05) in the catalpol (100 μM) treated group under H2O2 (200 μM) induced oxidative stress, whereasnumbers of apoptoic nuclei were significantly reduced (P<0.05). In conclusion, our results indicate that treatment ofcatalpol may have important implications for improving developmental competence and preimplantation quality ofporcine embryos through its anti-oxidant and anti-apoptotic effect.
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